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The map label for this gene is sir1 [H]

Identifier: 92114014

GI number: 92114014

Start: 2150853

End: 2152505

Strand: Direct

Name: sir1 [H]

Synonym: Csal_1891

Alternate gene names: 92114014

Gene position: 2150853-2152505 (Clockwise)

Preceding gene: 92114013

Following gene: 92114015

Centisome position: 58.18

GC content: 62.25

Gene sequence:

>1653_bases
ATGTATCGGTACGACTCGTACGACCAAACACTCGTCGATGAACGTGTCGCGCAGTTCCGCGACCAGATGCGCCGCTATCT
CGAGGGCAAGATCAGCGATGAGGAATTCCTGCCGCTGCGCGTGCAGAACGGCCTCTACGTGCAGCGTTATGCCCCCATGC
TGCGCATTGCCATTCCCTACGGCATGCTGGCGTCCTACCAGTTGCGCAAGCTGGGCGAGATCGCTGCACGCTACGATCGC
GGCTATGGCCATTTCACCACGCGCACCAACCTGCAGCTGAACTGGCCCAAGCTCGAGGACGTACCCGACATCCTGGCGGA
TCTGGCCAGCGTGCAGATGCATGCCATTCAGACCAGCGGCAACGATATCCGCAACACCACGACCGATCAGTTCTCGGGCA
TCGCCGCCGACGAGGATGTCGATCCGCGTCCCTGGTGCGAACTGATCCGTCAGTGGTCGACCTTCCACCCGGAATTCGCC
TATCTGCCGCGCAAGTTCAAGATCGCCGTGACCGGTGCCGAGGAAGACCGCGCCGCCATCCAGGTGCACGACGTGGGTCT
GCGCTTCTGGCGCAACGACGACGGCGATGTCCGCGTCAAGGTGCTGGCCGGCGGTGGCCTCGGACGCACCCCGATGATCG
GTGCCGTGATGTGCGACGATCTGCCGTGGCAGCACCTGCTCACGTATCTCGAAGCCATCCTGCGTGTCTACAACCAGTAC
GGCCGCCGCGACAACAAGTTCAAGGCGCGTATCAAGATTCTGGTCAAGGCGCTGGGCATCGATGAGTTCCGGCGCCGTGT
CGAGGCCGAATGGGCACATCTCAAGGACGGCCCGCAGACGTTGACCGAGGAAGCGATCTCCGCCGTGGCCGGGCACTTCG
TCGAACCGCCGCGTCGCGACGTCTCCGAGGACGCCATCGCGGACTTCGAACGCCTGCGCAGTGAAAATCGTGCCTTCGCG
CGTTTCGTCACCAACAACGTCACCGATCACAAGGTGCGCGGATACAAGGCCGTGACCCTGTCGCTCAAGCGTCACGATCA
TTCGCCCGGCGACGTGACCTCCGCAGACATGGCCGCCGTGGCCGACCTGGCCGACGAATTCGGCTATGGCGAGGTGCGCG
TTACCCACGAGCAGAATCTGGTGCTGACCGACGTTCCGGTGGACCGCATGGAGGAGCTCTGGCAGAAGCTCGAGGCACTC
GGCATGGCCAACCCGACCGTCGGCACCCTGGCCGATTTGATCTGCTGCCCGGGGGGCGATTACTGCTCGCTGGCCAACGC
CAAGTCGATTCCCGTCGCCCAGTCGATCCAGGAACGTTTCGAGGATCTCGATTTCCTCTATGACCTGGGACCGCTGGAGC
TCAACATCTCGGGCTGCATGAATGCCTGCGGTCACCACCACGTGGGCCACATCGGCATCCTCGGGGTCGACAAGAAAGGC
GAAGAGTACTATCAGATTTCGCTGGGCGGCAGCCAGGGCAACGAATCGTCTCTGGGCAAGATTCTGGGCCCCTCCTTCTA
TCGCGAGGATGTCCCGGATGTCATCGACAAGCTGTTGAAGGTGTACGTCGGGGAACGCACGGCCGACGAGACCTTCCTCG
ACACGTATCGTCGCATCGGCCTGAAACCTTTCAAGGAGCATGTCTATGCCTGA

Upstream 100 bases:

>100_bases
AAAGCAATAACCTATTATTATTTATTCTTTCAAAGAATATAGAAGGCAGGGTAAGGTGCCCTGCTACGGCAATCCATGTG
ACTAGCAGGACACCGCCTGT

Downstream 100 bases:

>100_bases
GCAGCGCACCCTGATCGTCGGTCGGCACGTCGTGGAAGACGACTGGACACTGCTTCTCGAAGACGAAGCGCCGCATTCCG
TGAACGGGCGTGCCATCGTC

Product: nitrite and sulfite reductase 4Fe-4S region

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 550; Mature: 550

Protein sequence:

>550_residues
MYRYDSYDQTLVDERVAQFRDQMRRYLEGKISDEEFLPLRVQNGLYVQRYAPMLRIAIPYGMLASYQLRKLGEIAARYDR
GYGHFTTRTNLQLNWPKLEDVPDILADLASVQMHAIQTSGNDIRNTTTDQFSGIAADEDVDPRPWCELIRQWSTFHPEFA
YLPRKFKIAVTGAEEDRAAIQVHDVGLRFWRNDDGDVRVKVLAGGGLGRTPMIGAVMCDDLPWQHLLTYLEAILRVYNQY
GRRDNKFKARIKILVKALGIDEFRRRVEAEWAHLKDGPQTLTEEAISAVAGHFVEPPRRDVSEDAIADFERLRSENRAFA
RFVTNNVTDHKVRGYKAVTLSLKRHDHSPGDVTSADMAAVADLADEFGYGEVRVTHEQNLVLTDVPVDRMEELWQKLEAL
GMANPTVGTLADLICCPGGDYCSLANAKSIPVAQSIQERFEDLDFLYDLGPLELNISGCMNACGHHHVGHIGILGVDKKG
EEYYQISLGGSQGNESSLGKILGPSFYREDVPDVIDKLLKVYVGERTADETFLDTYRRIGLKPFKEHVYA

Sequences:

>Translated_550_residues
MYRYDSYDQTLVDERVAQFRDQMRRYLEGKISDEEFLPLRVQNGLYVQRYAPMLRIAIPYGMLASYQLRKLGEIAARYDR
GYGHFTTRTNLQLNWPKLEDVPDILADLASVQMHAIQTSGNDIRNTTTDQFSGIAADEDVDPRPWCELIRQWSTFHPEFA
YLPRKFKIAVTGAEEDRAAIQVHDVGLRFWRNDDGDVRVKVLAGGGLGRTPMIGAVMCDDLPWQHLLTYLEAILRVYNQY
GRRDNKFKARIKILVKALGIDEFRRRVEAEWAHLKDGPQTLTEEAISAVAGHFVEPPRRDVSEDAIADFERLRSENRAFA
RFVTNNVTDHKVRGYKAVTLSLKRHDHSPGDVTSADMAAVADLADEFGYGEVRVTHEQNLVLTDVPVDRMEELWQKLEAL
GMANPTVGTLADLICCPGGDYCSLANAKSIPVAQSIQERFEDLDFLYDLGPLELNISGCMNACGHHHVGHIGILGVDKKG
EEYYQISLGGSQGNESSLGKILGPSFYREDVPDVIDKLLKVYVGERTADETFLDTYRRIGLKPFKEHVYA
>Mature_550_residues
MYRYDSYDQTLVDERVAQFRDQMRRYLEGKISDEEFLPLRVQNGLYVQRYAPMLRIAIPYGMLASYQLRKLGEIAARYDR
GYGHFTTRTNLQLNWPKLEDVPDILADLASVQMHAIQTSGNDIRNTTTDQFSGIAADEDVDPRPWCELIRQWSTFHPEFA
YLPRKFKIAVTGAEEDRAAIQVHDVGLRFWRNDDGDVRVKVLAGGGLGRTPMIGAVMCDDLPWQHLLTYLEAILRVYNQY
GRRDNKFKARIKILVKALGIDEFRRRVEAEWAHLKDGPQTLTEEAISAVAGHFVEPPRRDVSEDAIADFERLRSENRAFA
RFVTNNVTDHKVRGYKAVTLSLKRHDHSPGDVTSADMAAVADLADEFGYGEVRVTHEQNLVLTDVPVDRMEELWQKLEAL
GMANPTVGTLADLICCPGGDYCSLANAKSIPVAQSIQERFEDLDFLYDLGPLELNISGCMNACGHHHVGHIGILGVDKKG
EEYYQISLGGSQGNESSLGKILGPSFYREDVPDVIDKLLKVYVGERTADETFLDTYRRIGLKPFKEHVYA

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6322597, Length=405, Percent_Identity=24.6913580246914, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.8.7.1 [H]

Molecular weight: Translated: 62310; Mature: 62310

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRYDSYDQTLVDERVAQFRDQMRRYLEGKISDEEFLPLRVQNGLYVQRYAPMLRIAIPY
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCEEEHHHCCHHHHHCCH
GMLASYQLRKLGEIAARYDRGYGHFTTRTNLQLNWPKLEDVPDILADLASVQMHAIQTSG
HHHHHHHHHHHHHHHHHHHCCCCCEEEECCEEECCCCCCCHHHHHHHHHHHHHHHEECCC
NDIRNTTTDQFSGIAADEDVDPRPWCELIRQWSTFHPEFAYLPRKFKIAVTGAEEDRAAI
CCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHCCCEEEEEEECCCCCCCEE
QVHDVGLRFWRNDDGDVRVKVLAGGGLGRTPMIGAVMCDDLPWQHLLTYLEAILRVYNQY
EEEECCEEEEECCCCCEEEEEEECCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHH
GRRDNKFKARIKILVKALGIDEFRRRVEAEWAHLKDGPQTLTEEAISAVAGHFVEPPRRD
CCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCC
VSEDAIADFERLRSENRAFARFVTNNVTDHKVRGYKAVTLSLKRHDHSPGDVTSADMAAV
CCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHH
ADLADEFGYGEVRVTHEQNLVLTDVPVDRMEELWQKLEALGMANPTVGTLADLICCPGGD
HHHHHHCCCCCEEEEECCCEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCC
YCSLANAKSIPVAQSIQERFEDLDFLYDLGPLELNISGCMNACGHHHVGHIGILGVDKKG
EECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHCCCCCCCEEEEEEECCCC
EEYYQISLGGSQGNESSLGKILGPSFYREDVPDVIDKLLKVYVGERTADETFLDTYRRIG
CEEEEEEECCCCCCHHHHHHHHCCHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
LKPFKEHVYA
CCHHHHHCCC
>Mature Secondary Structure
MYRYDSYDQTLVDERVAQFRDQMRRYLEGKISDEEFLPLRVQNGLYVQRYAPMLRIAIPY
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCEEEHHHCCHHHHHCCH
GMLASYQLRKLGEIAARYDRGYGHFTTRTNLQLNWPKLEDVPDILADLASVQMHAIQTSG
HHHHHHHHHHHHHHHHHHHCCCCCEEEECCEEECCCCCCCHHHHHHHHHHHHHHHEECCC
NDIRNTTTDQFSGIAADEDVDPRPWCELIRQWSTFHPEFAYLPRKFKIAVTGAEEDRAAI
CCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHCCCEEEEEEECCCCCCCEE
QVHDVGLRFWRNDDGDVRVKVLAGGGLGRTPMIGAVMCDDLPWQHLLTYLEAILRVYNQY
EEEECCEEEEECCCCCEEEEEEECCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHH
GRRDNKFKARIKILVKALGIDEFRRRVEAEWAHLKDGPQTLTEEAISAVAGHFVEPPRRD
CCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCC
VSEDAIADFERLRSENRAFARFVTNNVTDHKVRGYKAVTLSLKRHDHSPGDVTSADMAAV
CCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHH
ADLADEFGYGEVRVTHEQNLVLTDVPVDRMEELWQKLEALGMANPTVGTLADLICCPGGD
HHHHHHCCCCCEEEEECCCEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCC
YCSLANAKSIPVAQSIQERFEDLDFLYDLGPLELNISGCMNACGHHHVGHIGILGVDKKG
EECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHCCCCCCCEEEEEEECCCC
EEYYQISLGGSQGNESSLGKILGPSFYREDVPDVIDKLLKVYVGERTADETFLDTYRRIG
CEEEEEEECCCCCCHHHHHHHHCCHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
LKPFKEHVYA
CCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA