Click here to switch to the map view.

The map label for this gene is tolB

Identifier: 92113976

GI number: 92113976

Start: 2107692

End: 2108978

Strand: Direct

Name: tolB

Synonym: Csal_1853

Alternate gene names: 92113976

Gene position: 2107692-2108978 (Clockwise)

Preceding gene: 92113975

Following gene: 92113977

Centisome position: 57.02

GC content: 61.62

Gene sequence:

>1287_bases
ATGAAACGACTCAATCTGTGGGCAGCTTCGTTGCTGTTAATGCTGTGTGCCGGCATGGCGCATGCCGAACTCACCATCGA
GATCACCAAGGGAAGCGATCAGGCGATTCCCATCGCCATCGTTCCCATGGCCAATGGCGACGATCTTCCCGAAAACGTCT
CGCGCATCGTCAGCAACGACCTCGAGCGTAGCGGCCGTTTCGAGCCGCTGCCGCGGGAGTCGCTGATTTCCCGTCCGAGC
AGTAGTGAAGAGGTTCACTATCAGGATTGGCAGCAGCTGGACAGCGACTATCTGGTCGTCGGCAAGATCGACAACCAGGG
CGGTGAATACCGGATCCAGTACGAACTGCTCAACGTGAATGGCCAAAACCGCATGCTCGGCGAGGTCGTGACCTCGAGTC
GCGACCAGCTGCGCGACAGCGCGCATTACATCAGCGATCAGATTTTCGAGGAAATCACCGGCATTCGCGGGGCATTTTCC
ACCAGCATCGCGTACGTCACCGCCAGCGGCGTCGGCGATGACATCGATTATGCCCTCAGCGTCGCCGATGCCGATGGCCA
CAATAGCCAGCAAATCCTGTCCTCGGACGAACCCATCCTGTCACCGGCCTGGTCACCGGATGGCGGCAAGCTGGCGTACG
TGTCTTTCGAAACCGGGCGCCCCGGCATCTATGTGCAGAATCTCGCCACCGGCCAGCGTGTCCGTCTGACCTCGTTCGAG
GGCATCAACGGTGCGCCGGCCTGGTCGCCAGACGGTCGCAAACTGGCGATGTCGCTGTCCAAGGACGGCCAGCCGGAAAT
CTATGTCATGGACATCGCGTCCCGCGATCTGCGGCGCCTGACGCAAAGCGACGCCATCGACACCGAGCCCGAATGGGCCC
CGAACGGCCGCAGCCTGATCTTCACGTCCGACCGCAGCGGCAACCCGCAGATCTATCGCTACGACCTGGGCAGCGGGCGG
GCCGAACGCCTCACCTTCACCGGCAGCTACAATTCACGTGGTCGTTTCAGCCCGGATGGAGACGCGATCTTCATGGTGAC
GCGTGACGACTCGGGCTATCACGTGGCGCGTCAGGATCTGGATACCGATCGCATGACGATTCTCACCGATTCCGATTGGG
ACGAGTCGCCCAGTGTTGCGCCGAACGGCACCATGGTAATCTTCGCCACCCAGGAAGGAACGCGCGGCGTGCTGGGGGCG
GTATCGGCCGATGGCAGCGCATCTTTCCGTCTGCCATCGCCGCAGGGCGACGTGCGGGAACCCGCATGGTCTCCCTTTTT
GGACTAA

Upstream 100 bases:

>100_bases
AAAGAGCTGCACCGTTTCGTGAAATGAGTGAACTCGACGCCTCGGTACAACGTCAGTTCCGTGAATTCAATCTGGACTTC
AACCCAGAGGACATCCGCTG

Downstream 100 bases:

>100_bases
CCCGCGTAATGTGTCATTCGATTTCCAAGTAAGGAGAAAACAATGCAATTCAAACCCTATGCCAAAGGATTCGCCGTCGC
CCTGTCGTTGGCGGTTCTCG

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MKRLNLWAASLLLMLCAGMAHAELTIEITKGSDQAIPIAIVPMANGDDLPENVSRIVSNDLERSGRFEPLPRESLISRPS
SSEEVHYQDWQQLDSDYLVVGKIDNQGGEYRIQYELLNVNGQNRMLGEVVTSSRDQLRDSAHYISDQIFEEITGIRGAFS
TSIAYVTASGVGDDIDYALSVADADGHNSQQILSSDEPILSPAWSPDGGKLAYVSFETGRPGIYVQNLATGQRVRLTSFE
GINGAPAWSPDGRKLAMSLSKDGQPEIYVMDIASRDLRRLTQSDAIDTEPEWAPNGRSLIFTSDRSGNPQIYRYDLGSGR
AERLTFTGSYNSRGRFSPDGDAIFMVTRDDSGYHVARQDLDTDRMTILTDSDWDESPSVAPNGTMVIFATQEGTRGVLGA
VSADGSASFRLPSPQGDVREPAWSPFLD

Sequences:

>Translated_428_residues
MKRLNLWAASLLLMLCAGMAHAELTIEITKGSDQAIPIAIVPMANGDDLPENVSRIVSNDLERSGRFEPLPRESLISRPS
SSEEVHYQDWQQLDSDYLVVGKIDNQGGEYRIQYELLNVNGQNRMLGEVVTSSRDQLRDSAHYISDQIFEEITGIRGAFS
TSIAYVTASGVGDDIDYALSVADADGHNSQQILSSDEPILSPAWSPDGGKLAYVSFETGRPGIYVQNLATGQRVRLTSFE
GINGAPAWSPDGRKLAMSLSKDGQPEIYVMDIASRDLRRLTQSDAIDTEPEWAPNGRSLIFTSDRSGNPQIYRYDLGSGR
AERLTFTGSYNSRGRFSPDGDAIFMVTRDDSGYHVARQDLDTDRMTILTDSDWDESPSVAPNGTMVIFATQEGTRGVLGA
VSADGSASFRLPSPQGDVREPAWSPFLD
>Mature_428_residues
MKRLNLWAASLLLMLCAGMAHAELTIEITKGSDQAIPIAIVPMANGDDLPENVSRIVSNDLERSGRFEPLPRESLISRPS
SSEEVHYQDWQQLDSDYLVVGKIDNQGGEYRIQYELLNVNGQNRMLGEVVTSSRDQLRDSAHYISDQIFEEITGIRGAFS
TSIAYVTASGVGDDIDYALSVADADGHNSQQILSSDEPILSPAWSPDGGKLAYVSFETGRPGIYVQNLATGQRVRLTSFE
GINGAPAWSPDGRKLAMSLSKDGQPEIYVMDIASRDLRRLTQSDAIDTEPEWAPNGRSLIFTSDRSGNPQIYRYDLGSGR
AERLTFTGSYNSRGRFSPDGDAIFMVTRDDSGYHVARQDLDTDRMTILTDSDWDESPSVAPNGTMVIFATQEGTRGVLGA
VSADGSASFRLPSPQGDVREPAWSPFLD

Specific function: Involved in the tonB-independent uptake of proteins

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family

Homologues:

Organism=Escherichia coli, GI1786961, Length=421, Percent_Identity=42.9928741092637, Blast_Score=343, Evalue=1e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOLB_CHRSD (Q1QWF3)

Other databases:

- EMBL:   CP000285
- RefSeq:   YP_573904.1
- ProteinModelPortal:   Q1QWF3
- SMR:   Q1QWF3
- STRING:   Q1QWF3
- GeneID:   4028373
- GenomeReviews:   CP000285_GR
- KEGG:   csa:Csal_1853
- NMPDR:   fig|290398.4.peg.1752
- eggNOG:   COG0823
- HOGENOM:   HBG644919
- OMA:   RVADYDG
- ProtClustDB:   PRK00178
- BioCyc:   CSAL290398:CSAL_1853-MONOMER
- HAMAP:   MF_00671
- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943
- Gene3D:   G3DSA:3.40.50.10070
- Gene3D:   G3DSA:2.130.10.10
- TIGRFAMs:   TIGR02800

Pfam domain/function: PF07676 PD40; PF04052 TolB_N; SSF52964 TolB_N

EC number: NA

Molecular weight: Translated: 46890; Mature: 46890

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLNLWAASLLLMLCAGMAHAELTIEITKGSDQAIPIAIVPMANGDDLPENVSRIVSND
CCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCCCEEEEEEEECCCCCCCHHHHHHHHHHH
LERSGRFEPLPRESLISRPSSSEEVHYQDWQQLDSDYLVVGKIDNQGGEYRIQYELLNVN
HHHCCCCCCCCHHHHHCCCCCCCCCCHHHHHHCCCCEEEEEEEECCCCCEEEEEEEEECC
GQNRMLGEVVTSSRDQLRDSAHYISDQIFEEITGIRGAFSTSIAYVTASGVGDDIDYALS
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCEEEEE
VADADGHNSQQILSSDEPILSPAWSPDGGKLAYVSFETGRPGIYVQNLATGQRVRLTSFE
EECCCCCCCHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCEEEEECCCCCEEEEEECC
GINGAPAWSPDGRKLAMSLSKDGQPEIYVMDIASRDLRRLTQSDAIDTEPEWAPNGRSLI
CCCCCCCCCCCCCEEEEEECCCCCCCEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCEEE
FTSDRSGNPQIYRYDLGSGRAERLTFTGSYNSRGRFSPDGDAIFMVTRDDSGYHVARQDL
EECCCCCCCCEEEEECCCCCEEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCEEECCCC
DTDRMTILTDSDWDESPSVAPNGTMVIFATQEGTRGVLGAVSADGSASFRLPSPQGDVRE
CCCCEEEEECCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEECCCCCEEECCCCCCCCCC
PAWSPFLD
CCCCCCCC
>Mature Secondary Structure
MKRLNLWAASLLLMLCAGMAHAELTIEITKGSDQAIPIAIVPMANGDDLPENVSRIVSND
CCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCCCEEEEEEEECCCCCCCHHHHHHHHHHH
LERSGRFEPLPRESLISRPSSSEEVHYQDWQQLDSDYLVVGKIDNQGGEYRIQYELLNVN
HHHCCCCCCCCHHHHHCCCCCCCCCCHHHHHHCCCCEEEEEEEECCCCCEEEEEEEEECC
GQNRMLGEVVTSSRDQLRDSAHYISDQIFEEITGIRGAFSTSIAYVTASGVGDDIDYALS
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCEEEEE
VADADGHNSQQILSSDEPILSPAWSPDGGKLAYVSFETGRPGIYVQNLATGQRVRLTSFE
EECCCCCCCHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCEEEEECCCCCEEEEEECC
GINGAPAWSPDGRKLAMSLSKDGQPEIYVMDIASRDLRRLTQSDAIDTEPEWAPNGRSLI
CCCCCCCCCCCCCEEEEEECCCCCCCEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCEEE
FTSDRSGNPQIYRYDLGSGRAERLTFTGSYNSRGRFSPDGDAIFMVTRDDSGYHVARQDL
EECCCCCCCCEEEEECCCCCEEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCEEECCCC
DTDRMTILTDSDWDESPSVAPNGTMVIFATQEGTRGVLGAVSADGSASFRLPSPQGDVRE
CCCCEEEEECCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEECCCCCEEECCCCCCCCCC
PAWSPFLD
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA