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The map label for this gene is cysL [H]

Identifier: 92113933

GI number: 92113933

Start: 2057298

End: 2058221

Strand: Direct

Name: cysL [H]

Synonym: Csal_1810

Alternate gene names: 92113933

Gene position: 2057298-2058221 (Clockwise)

Preceding gene: 92113930

Following gene: 92113937

Centisome position: 55.65

GC content: 60.5

Gene sequence:

>924_bases
ATGTTCAACGCGCAATACTTTCGCACCTTCATCACGCTTGTCGAGACGGGTAGCTTCACGCACACCGCACGCAAGCTGGA
AATGACCCAGCCTGGCGTCAGCCAGCACATCCGCAAGCTCGAACGCTACCTTGACAAGTCATTGCTGTATCGTCATGGCC
GTCGCTTCACGCTCACGGAAACCGGACGCCGTGCCTATGACTATGCGGTCAAGCTTTTTGCCGAGCATGAGCAGTTTCGG
CATTCCCTGGACAACGATTCGCCGGACACGGGCGAATGCCGCGTTGCGTCGCCGAGCAGCGTGGGACTGATGTTCTATCC
GTTCATCCTGGGCTACCAGCAGATGCACCCGGGATTGACGGTGACCTACGATTTCGCCTTCAACGCGGAGATCGTCGACG
CCGTATTGGCCGGACGCTTCGATGTCGGCATCGTCACGGACGTGACCAAGCATCCCGACCTGGATTTCGAACCCTGGCAT
CAGGAGCCGCTGTGCCTGCTGGTGCCCGCCGACTTCACGGGCACCACGATCAGCGAACTCATGGCGCTCGGTTTCATGAA
CTATTCGGACGGCATCAATAACGCCAGTTCGTTGCTCAGGCGCAATTTCGCGGGCGAGTTTCGCTCGATGAGCCACTTTC
CCCAGCAGGGCTTCTCCAACGAAGTCAGCATGGTGCTGGATGCAGTGGCACGAGGGCTGGGCTTTACCGTGGTACCTCGG
AGCGTGATGGAAACCTCGCCCTGGCAGCGTCAGGTCAAGGAGCTGCCCCTGGCGGAGTCGGTCTACGAGACGCTGTATGT
CGTCACCAAGCGCGGCGTCGAGATGCCGCGTCGCTACCAGCAGCTGCTGGGCGAGTTTCGCCTGCAGCGTCGCAACGTCC
TGTACGGCTATGCCGAAGCGCCGCCGCTCGAAGACGAGGCCTGA

Upstream 100 bases:

>100_bases
CCACGGTTATGGCCGTGCTAACCGTTACGACGGTCGTGCGTCAGCAGGCTTCGGCATAGATGCAGCCGCCCCGATACGCC
AGCCGGTGGACCCTCAGTTC

Downstream 100 bases:

>100_bases
CCGGAACGATCATGCCTAAAGCGTAGTCTTCGTCTTCGCTGAACCCCGGGCCTTGCCGCCCGGGGTTTTTCGTCGGTCCT
GTCGAAACGTTCAGACGTCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: CysJI operon transcriptional activator [H]

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MFNAQYFRTFITLVETGSFTHTARKLEMTQPGVSQHIRKLERYLDKSLLYRHGRRFTLTETGRRAYDYAVKLFAEHEQFR
HSLDNDSPDTGECRVASPSSVGLMFYPFILGYQQMHPGLTVTYDFAFNAEIVDAVLAGRFDVGIVTDVTKHPDLDFEPWH
QEPLCLLVPADFTGTTISELMALGFMNYSDGINNASSLLRRNFAGEFRSMSHFPQQGFSNEVSMVLDAVARGLGFTVVPR
SVMETSPWQRQVKELPLAESVYETLYVVTKRGVEMPRRYQQLLGEFRLQRRNVLYGYAEAPPLEDEA

Sequences:

>Translated_307_residues
MFNAQYFRTFITLVETGSFTHTARKLEMTQPGVSQHIRKLERYLDKSLLYRHGRRFTLTETGRRAYDYAVKLFAEHEQFR
HSLDNDSPDTGECRVASPSSVGLMFYPFILGYQQMHPGLTVTYDFAFNAEIVDAVLAGRFDVGIVTDVTKHPDLDFEPWH
QEPLCLLVPADFTGTTISELMALGFMNYSDGINNASSLLRRNFAGEFRSMSHFPQQGFSNEVSMVLDAVARGLGFTVVPR
SVMETSPWQRQVKELPLAESVYETLYVVTKRGVEMPRRYQQLLGEFRLQRRNVLYGYAEAPPLEDEA
>Mature_307_residues
MFNAQYFRTFITLVETGSFTHTARKLEMTQPGVSQHIRKLERYLDKSLLYRHGRRFTLTETGRRAYDYAVKLFAEHEQFR
HSLDNDSPDTGECRVASPSSVGLMFYPFILGYQQMHPGLTVTYDFAFNAEIVDAVLAGRFDVGIVTDVTKHPDLDFEPWH
QEPLCLLVPADFTGTTISELMALGFMNYSDGINNASSLLRRNFAGEFRSMSHFPQQGFSNEVSMVLDAVARGLGFTVVPR
SVMETSPWQRQVKELPLAESVYETLYVVTKRGVEMPRRYQQLLGEFRLQRRNVLYGYAEAPPLEDEA

Specific function: Transcriptional activator of the cysJI operon which is involved in sulfur assimilation. Also negatively regulates its own transcription [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787806, Length=274, Percent_Identity=24.4525547445255, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI145693105, Length=272, Percent_Identity=23.8970588235294, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI157672245, Length=277, Percent_Identity=25.2707581227437, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1788481, Length=251, Percent_Identity=23.9043824701195, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI87082132, Length=259, Percent_Identity=25.4826254826255, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788887, Length=246, Percent_Identity=22.3577235772358, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1790208, Length=252, Percent_Identity=25, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35125; Mature: 35125

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNAQYFRTFITLVETGSFTHTARKLEMTQPGVSQHIRKLERYLDKSLLYRHGRRFTLTE
CCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEC
TGRRAYDYAVKLFAEHEQFRHSLDNDSPDTGECRVASPSSVGLMFYPFILGYQQMHPGLT
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCE
VTYDFAFNAEIVDAVLAGRFDVGIVTDVTKHPDLDFEPWHQEPLCLLVPADFTGTTISEL
EEEEEEECHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHH
MALGFMNYSDGINNASSLLRRNFAGEFRSMSHFPQQGFSNEVSMVLDAVARGLGFTVVPR
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCEECCH
SVMETSPWQRQVKELPLAESVYETLYVVTKRGVEMPRRYQQLLGEFRLQRRNVLYGYAEA
HHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEEEEECCC
PPLEDEA
CCCCCCC
>Mature Secondary Structure
MFNAQYFRTFITLVETGSFTHTARKLEMTQPGVSQHIRKLERYLDKSLLYRHGRRFTLTE
CCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEC
TGRRAYDYAVKLFAEHEQFRHSLDNDSPDTGECRVASPSSVGLMFYPFILGYQQMHPGLT
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCE
VTYDFAFNAEIVDAVLAGRFDVGIVTDVTKHPDLDFEPWHQEPLCLLVPADFTGTTISEL
EEEEEEECHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHH
MALGFMNYSDGINNASSLLRRNFAGEFRSMSHFPQQGFSNEVSMVLDAVARGLGFTVVPR
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCEECCH
SVMETSPWQRQVKELPLAESVYETLYVVTKRGVEMPRRYQQLLGEFRLQRRNVLYGYAEA
HHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEEEEECCC
PPLEDEA
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]