Click here to switch to the map view.

The map label for this gene is dsbA [H]

Identifier: 91790320

GI number: 91790320

Start: 4742611

End: 4743273

Strand: Reverse

Name: dsbA [H]

Synonym: Bpro_4488

Alternate gene names: 91790320

Gene position: 4743273-4742611 (Counterclockwise)

Preceding gene: 91790321

Following gene: 91790319

Centisome position: 91.21

GC content: 63.2

Gene sequence:

>663_bases
ATGCAACGACGTGAGTTTTCGCTTTCAGCCGCTACCGTGGCAGCCGCCGCCACCTGGGGGGGCGTTGCGCTGCCCACGGC
GGCGCAGGCCCAGGCCCGCGCCTTCAAGGAGGGATCGGATTATTTGACGCTGGGCAAGCCCGCCCCCACCGACGTGCCTG
CCGGCCAGGTCGAAGTGGTGGAATTCTTCTGGTACAGCTGCCCGCATTGCAATGCGTTCGAGCCAGCGCTGGAGGCCTGG
ATCCAGAAAGCCCCCAAAGACGTGACCGTGCGGCGCGTGCCGGTGGCTTTCCGTCCCGACTTCGAGCCGCAGCAGCGTCT
TTTTTATGTGCTCGAAGGCATGGGCAAGCTCAATGACCTGCACAAAAAGGTGTTCAACGCAATCCATGTGGAGCGTCAGG
CGCTGAACACCGGGGATCAGGTCGCGGCCTGGGCGGAAAAGCAGGGCCTGAACAAGGCCAGGTTTGTCGAGATGTACAAC
TCGTTTTCCGTCAGTACCAAGGCCCGCAAGGCCACCCAGTTGCAGGATACCTATGCGGTGGACGGCGTGCCGGCCCTGGG
CATCGGCGGGCGTTTCTACACGTCTGGCCAGCTGGCCCAGAACATGGACCGCGCGCTCCAGGTCACCGACTACCTGATCG
GACAATCGCGCAAGGCCAAGTGA

Upstream 100 bases:

>100_bases
GCGGCGGTGTACCAACCCCTGCACGACATCGCCGGCCGGTGGCCATCGCATGGAAAGACCGGCTGCCTTGCAACCCCCAG
ACATATCAGGAGAAAAATTG

Downstream 100 bases:

>100_bases
CCCCCCGGCAAGGGTGAGGCCAGGCGCCGGCGCCCGCCACCAGCCGAAAAACCCGCCCAGCCGTGGCGGGTTTTTTTATG
GGCCCATTTGCCGGCGCCGG

Product: DSBA oxidoreductase

Products: 3-oxo-D 4-steroid; protein with oxidized disulfide bonds [C]

Alternate protein names: NA

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MQRREFSLSAATVAAAATWGGVALPTAAQAQARAFKEGSDYLTLGKPAPTDVPAGQVEVVEFFWYSCPHCNAFEPALEAW
IQKAPKDVTVRRVPVAFRPDFEPQQRLFYVLEGMGKLNDLHKKVFNAIHVERQALNTGDQVAAWAEKQGLNKARFVEMYN
SFSVSTKARKATQLQDTYAVDGVPALGIGGRFYTSGQLAQNMDRALQVTDYLIGQSRKAK

Sequences:

>Translated_220_residues
MQRREFSLSAATVAAAATWGGVALPTAAQAQARAFKEGSDYLTLGKPAPTDVPAGQVEVVEFFWYSCPHCNAFEPALEAW
IQKAPKDVTVRRVPVAFRPDFEPQQRLFYVLEGMGKLNDLHKKVFNAIHVERQALNTGDQVAAWAEKQGLNKARFVEMYN
SFSVSTKARKATQLQDTYAVDGVPALGIGGRFYTSGQLAQNMDRALQVTDYLIGQSRKAK
>Mature_220_residues
MQRREFSLSAATVAAAATWGGVALPTAAQAQARAFKEGSDYLTLGKPAPTDVPAGQVEVVEFFWYSCPHCNAFEPALEAW
IQKAPKDVTVRRVPVAFRPDFEPQQRLFYVLEGMGKLNDLHKKVFNAIHVERQALNTGDQVAAWAEKQGLNKARFVEMYN
SFSVSTKARKATQLQDTYAVDGVPALGIGGRFYTSGQLAQNMDRALQVTDYLIGQSRKAK

Specific function: Involved in disulfide-bond formation. Acts by transferring its disulfide bond to other proteins [H]

COG id: COG0526

COG function: function code OC; Thiol-disulfide isomerase and thioredoxins

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

None

Paralogues:

None

Copy number: 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1348 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001853
- InterPro:   IPR023205
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR013766
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01323 DSBA; PF00085 Thioredoxin [H]

EC number: NA

Molecular weight: Translated: 24273; Mature: 24273

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRREFSLSAATVAAAATWGGVALPTAAQAQARAFKEGSDYLTLGKPAPTDVPAGQVEVV
CCCCHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHH
EFFWYSCPHCNAFEPALEAWIQKAPKDVTVRRVPVAFRPDFEPQQRLFYVLEGMGKLNDL
HHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEEECEEECCCCCHHHHHHHHHHCCHHHHHH
HKKVFNAIHVERQALNTGDQVAAWAEKQGLNKARFVEMYNSFSVSTKARKATQLQDTYAV
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHC
DGVPALGIGGRFYTSGQLAQNMDRALQVTDYLIGQSRKAK
CCCCEECCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MQRREFSLSAATVAAAATWGGVALPTAAQAQARAFKEGSDYLTLGKPAPTDVPAGQVEVV
CCCCHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHH
EFFWYSCPHCNAFEPALEAWIQKAPKDVTVRRVPVAFRPDFEPQQRLFYVLEGMGKLNDL
HHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEEECEEECCCCCHHHHHHHHHHCCHHHHHH
HKKVFNAIHVERQALNTGDQVAAWAEKQGLNKARFVEMYNSFSVSTKARKATQLQDTYAV
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHC
DGVPALGIGGRFYTSGQLAQNMDRALQVTDYLIGQSRKAK
CCCCEECCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxo-D 5-steroid; protein with reduced SH groups [C]

Specific reaction: 3-oxo-D 5-steroid = 3-oxo-D 4-steroid protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: Isomerases; Intramolecular Oxidoreductases; Transposing S-S Bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12910271 [H]