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The map label for this gene is cobA [H]

Identifier: 91789141

GI number: 91789141

Start: 3477358

End: 3478182

Strand: Direct

Name: cobA [H]

Synonym: Bpro_3281

Alternate gene names: 91789141

Gene position: 3477358-3478182 (Clockwise)

Preceding gene: 91789140

Following gene: 91789142

Centisome position: 66.87

GC content: 67.27

Gene sequence:

>825_bases
ATGAAAAACAGCAAGGTCCGCCCCACCCCCGATGGTGAACACGTCCTGGTAGCCGGCGGCAGATGCCTGCTTGTGGGCGC
TGGCCCGGGCGACCCGGAGCTGCTCACCCTCAAGGCCCTCAAGGCCATCCAGAATGCCACCGTGCTGCTGGTCGACGACC
TCGTCAGCGACGAGATCGTCGCGTTTGCGCGGCCCGACGCACGCATCGTCCATGTGGGCAAGCGCGGCGGCTGCAAATCC
ACCCCGCAGGCCTTCATCGAAAAGCTCATGATCATGGCGGCCCGCGAAGGCGAAAACGTCGTGCGCCTCAAGGGCGGTGA
TCCCTTCATCTTCGGCCGCGGTGGCGAGGAAGTCGAGCACCTGAAGGCTGCCGGCGTGGAGGTGGAGGTCATCAACGGCA
TCACTGCCGGGCTGGCGGCGGTGACCTCGCTGGGCGTGCCGCTCACGCACCGCCAGCATGCACATGGCGTGGTGTTTGTG
ACCGGCCACGCCAAGCCCGGCGATGCCGGCACCGACTGGGCCGCACTGGCGGCAACGGCCGCGCAGGCCAGGCTCACCCT
GGTGATCTACATGGGCGTGACGGGTTCACAGCACATCCAGGACCAGCTGTTGCAGGGCCTGCCCGCGGACACCCCAGTGG
CCATCATCCAGAACGCCAGCCTGCCGGGCCAGCGCCATGCAATCACCACCCTGGCCACCATGCGCACCACGATAGCTCGC
GAGCACCTGCAGAGCCCCAGCGTGATCGTGGTGGGCGATGTACTGCGCGGCGTGGCCCTGCTGGCCGAGCATCCGGAAGA
GCAGAAGCTGCGGGAGTTTGGCTGA

Upstream 100 bases:

>100_bases
GTACCGCCGCGTATATCCGCGCGGTCCTCTCGGGTACACATGCCGTGCCCGCATCGATTGCCCGGCAGGTGGAACATATC
TTGCAACTGGTCTCGCAATT

Downstream 100 bases:

>100_bases
GGCTCTGAGACACTAAGGCGCTGCGGCTCTGAGCCCTGCAGTCCACGGTGTCCGTTGAGACATGCTGAGGCATGCGGTGG
CGCGCAACGGCCTGCGGTGC

Product: uroporphyrinogen-III C-methyltransferase

Products: NA

Alternate protein names: Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM [H]

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MKNSKVRPTPDGEHVLVAGGRCLLVGAGPGDPELLTLKALKAIQNATVLLVDDLVSDEIVAFARPDARIVHVGKRGGCKS
TPQAFIEKLMIMAAREGENVVRLKGGDPFIFGRGGEEVEHLKAAGVEVEVINGITAGLAAVTSLGVPLTHRQHAHGVVFV
TGHAKPGDAGTDWAALAATAAQARLTLVIYMGVTGSQHIQDQLLQGLPADTPVAIIQNASLPGQRHAITTLATMRTTIAR
EHLQSPSVIVVGDVLRGVALLAEHPEEQKLREFG

Sequences:

>Translated_274_residues
MKNSKVRPTPDGEHVLVAGGRCLLVGAGPGDPELLTLKALKAIQNATVLLVDDLVSDEIVAFARPDARIVHVGKRGGCKS
TPQAFIEKLMIMAAREGENVVRLKGGDPFIFGRGGEEVEHLKAAGVEVEVINGITAGLAAVTSLGVPLTHRQHAHGVVFV
TGHAKPGDAGTDWAALAATAAQARLTLVIYMGVTGSQHIQDQLLQGLPADTPVAIIQNASLPGQRHAITTLATMRTTIAR
EHLQSPSVIVVGDVLRGVALLAEHPEEQKLREFG
>Mature_274_residues
MKNSKVRPTPDGEHVLVAGGRCLLVGAGPGDPELLTLKALKAIQNATVLLVDDLVSDEIVAFARPDARIVHVGKRGGCKS
TPQAFIEKLMIMAAREGENVVRLKGGDPFIFGRGGEEVEHLKAAGVEVEVINGITAGLAAVTSLGVPLTHRQHAHGVVFV
TGHAKPGDAGTDWAALAATAAQARLTLVIYMGVTGSQHIQDQLLQGLPADTPVAIIQNASLPGQRHAITTLATMRTTIAR
EHLQSPSVIVVGDVLRGVALLAEHPEEQKLREFG

Specific function: Catalyzes both methylations at C-2 and C-7 of uroporphyrinogen III leading to precorrin-1 and precorrin-2; their oxidative esterification gives respectively factor I octamethyl ester and sirohydrochlorin [H]

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=237, Percent_Identity=46.4135021097046, Blast_Score=193, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6322922, Length=233, Percent_Identity=31.7596566523605, Blast_Score=101, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.107 [H]

Molecular weight: Translated: 28788; Mature: 28788

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNSKVRPTPDGEHVLVAGGRCLLVGAGPGDPELLTLKALKAIQNATVLLVDDLVSDEIV
CCCCCCCCCCCCCEEEEECCEEEEEECCCCCCCEEHHHHHHHHCCCEEEEEECCCCCCEE
AFARPDARIVHVGKRGGCKSTPQAFIEKLMIMAAREGENVVRLKGGDPFIFGRGGEEVEH
EEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCEEEECCCCCHHHH
LKAAGVEVEVINGITAGLAAVTSLGVPLTHRQHAHGVVFVTGHAKPGDAGTDWAALAATA
HHHCCCEEEEECCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHH
AQARLTLVIYMGVTGSQHIQDQLLQGLPADTPVAIIQNASLPGQRHAITTLATMRTTIAR
HHCEEEEEEEECCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHH
EHLQSPSVIVVGDVLRGVALLAEHPEEQKLREFG
HHHCCCCEEEEHHHHHHHHHHHCCCCHHHHHHCC
>Mature Secondary Structure
MKNSKVRPTPDGEHVLVAGGRCLLVGAGPGDPELLTLKALKAIQNATVLLVDDLVSDEIV
CCCCCCCCCCCCCEEEEECCEEEEEECCCCCCCEEHHHHHHHHCCCEEEEEECCCCCCEE
AFARPDARIVHVGKRGGCKSTPQAFIEKLMIMAAREGENVVRLKGGDPFIFGRGGEEVEH
EEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCEEEECCCCCHHHH
LKAAGVEVEVINGITAGLAAVTSLGVPLTHRQHAHGVVFVTGHAKPGDAGTDWAALAATA
HHHCCCEEEEECCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHH
AQARLTLVIYMGVTGSQHIQDQLLQGLPADTPVAIIQNASLPGQRHAITTLATMRTTIAR
HHCEEEEEEEECCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHH
EHLQSPSVIVVGDVLRGVALLAEHPEEQKLREFG
HHHCCCCEEEEHHHHHHHHHHHCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]