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The map label for this gene is garL [H]

Identifier: 91788963

GI number: 91788963

Start: 3275506

End: 3276288

Strand: Reverse

Name: garL [H]

Synonym: Bpro_3103

Alternate gene names: 91788963

Gene position: 3276288-3275506 (Counterclockwise)

Preceding gene: 91788964

Following gene: 91788962

Centisome position: 63.0

GC content: 64.5

Gene sequence:

>783_bases
ATGACCCAAACCACGCCTTACCAATCCTTTCCCAACAGTTTCAAACGCGACCTGCTGGCCGGGAAGCAACTGATCGGCTG
CTGGTCGTCGCTGTCCAACGCCATAACCACCGAGGTCCTCGGCGTGGCCGGCTTTGACTGGGTGCTGCTGGACGGTGAGC
ATTCGCCCAACGACATGGCCACCTTCATTCCGCAGCTGATGGCGCTCAAGGACAGCGTGACTGCGCCCGTGGTGCGGCCT
TCGTCCAACAACGTGGTTGAAATCAAGCGGCTGCTGGACGCCGGTTTTTACAACTTCCTCATCCCCTTCGTCGAGAGCGC
CGATGAAGCGCGGCGCGCTGTCTCTGCCACGCGTTACCCGCCGCAGGGCATTCGGGGCGTGTCGGTGTCCCAGCGCAGCA
ACCGGTATGGCACGGTGGTCGATTATTTGAAGCTGATCAACGAGCAGATCTGCGTGATGGTGCAGATCGAGAGCCTTGCC
GGTATCGCCGCCACGCGCGAAATTGCAGCGCTGGACGGGGTGGATTGCATCTTCGTCGGCCCCTCGGACCTGGCGGCCGG
CCTGGGCCACCTGGGCAATGCGGGCCACCCCGAAGTGCAGGCCGCCATTGCCGCCGTTTTTGCCGATGCCAAGGCGGCCG
GCAAGCCCATCGGCATCCTGGCACCCGTCGAAGCCGATGCGCGCCGCTACATGGAGATGGGCGCCACCTTTGTGGGCGTC
GGCAGCGACCTGGGCACCTTCCGCTCGGCCACGCAGGCCCTGCGCGACCGTTACCGCGCATGA

Upstream 100 bases:

>100_bases
TGGGCGGAGCACTGGAAGCTGCACAACGCGCTGGTGCTTTTCAATCCGGCACCCATTACCTGATCCGAACCTGAGCCTCA
AGTTTCAAGGAAGAATTCAC

Downstream 100 bases:

>100_bases
CGGATATTTCTTCAATAACTTGGACTGGAGTCACAAAATGAGCAAGCTTGGTTTTATCGGCCTGGGCATCATGGGATCGC
CGATGGCGTCCCACCTGATC

Product: alpha-dehydro-beta-deoxy-D-glucarate aldolase

Products: NA

Alternate protein names: KDGluc aldolase; KDGlucA; 2-dehydro-3-deoxy-D-glucarate aldolase; 2-keto-3-deoxy-D-glucarate aldolase; 5-dehydro-4-deoxy-D-glucarate aldolase; Alpha-keto-beta-deoxy-D-glucarate aldolase [H]

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MTQTTPYQSFPNSFKRDLLAGKQLIGCWSSLSNAITTEVLGVAGFDWVLLDGEHSPNDMATFIPQLMALKDSVTAPVVRP
SSNNVVEIKRLLDAGFYNFLIPFVESADEARRAVSATRYPPQGIRGVSVSQRSNRYGTVVDYLKLINEQICVMVQIESLA
GIAATREIAALDGVDCIFVGPSDLAAGLGHLGNAGHPEVQAAIAAVFADAKAAGKPIGILAPVEADARRYMEMGATFVGV
GSDLGTFRSATQALRDRYRA

Sequences:

>Translated_260_residues
MTQTTPYQSFPNSFKRDLLAGKQLIGCWSSLSNAITTEVLGVAGFDWVLLDGEHSPNDMATFIPQLMALKDSVTAPVVRP
SSNNVVEIKRLLDAGFYNFLIPFVESADEARRAVSATRYPPQGIRGVSVSQRSNRYGTVVDYLKLINEQICVMVQIESLA
GIAATREIAALDGVDCIFVGPSDLAAGLGHLGNAGHPEVQAAIAAVFADAKAAGKPIGILAPVEADARRYMEMGATFVGV
GSDLGTFRSATQALRDRYRA
>Mature_259_residues
TQTTPYQSFPNSFKRDLLAGKQLIGCWSSLSNAITTEVLGVAGFDWVLLDGEHSPNDMATFIPQLMALKDSVTAPVVRPS
SNNVVEIKRLLDAGFYNFLIPFVESADEARRAVSATRYPPQGIRGVSVSQRSNRYGTVVDYLKLINEQICVMVQIESLAG
IAATREIAALDGVDCIFVGPSDLAAGLGHLGNAGHPEVQAAIAAVFADAKAAGKPIGILAPVEADARRYMEMGATFVGVG
SDLGTFRSATQALRDRYRA

Specific function: Catalyzes the reversible retro-aldol cleavage of both 5- keto-4-deoxy-D-glucarate and 2-keto-3-deoxy-D-glucarate to pyruvate and tartronic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. KDGluc aldolase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789514, Length=250, Percent_Identity=67.6, Blast_Score=349, Evalue=1e-97,
Organism=Escherichia coli, GI1788578, Length=240, Percent_Identity=41.6666666666667, Blast_Score=196, Evalue=1e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR017648
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: =4.1.2.20 [H]

Molecular weight: Translated: 27794; Mature: 27663

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS00012 PHOSPHOPANTETHEINE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQTTPYQSFPNSFKRDLLAGKQLIGCWSSLSNAITTEVLGVAGFDWVLLDGEHSPNDMA
CCCCCCCHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHH
TFIPQLMALKDSVTAPVVRPSSNNVVEIKRLLDAGFYNFLIPFVESADEARRAVSATRYP
HHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCC
PQGIRGVSVSQRSNRYGTVVDYLKLINEQICVMVQIESLAGIAATREIAALDGVDCIFVG
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEEEEEHHHHHHHHHHHHHHHCCCCEEEEC
PSDLAAGLGHLGNAGHPEVQAAIAAVFADAKAAGKPIGILAPVEADARRYMEMGATFVGV
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHCCEEEEC
GSDLGTFRSATQALRDRYRA
CCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TQTTPYQSFPNSFKRDLLAGKQLIGCWSSLSNAITTEVLGVAGFDWVLLDGEHSPNDMA
CCCCCCHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHH
TFIPQLMALKDSVTAPVVRPSSNNVVEIKRLLDAGFYNFLIPFVESADEARRAVSATRYP
HHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCC
PQGIRGVSVSQRSNRYGTVVDYLKLINEQICVMVQIESLAGIAATREIAALDGVDCIFVG
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEEEEEHHHHHHHHHHHHHHHCCCCEEEEC
PSDLAAGLGHLGNAGHPEVQAAIAAVFADAKAAGKPIGILAPVEADARRYMEMGATFVGV
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHCCEEEEC
GSDLGTFRSATQALRDRYRA
CCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA