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The map label for this gene is dmlA [H]

Identifier: 91788926

GI number: 91788926

Start: 3231826

End: 3232944

Strand: Reverse

Name: dmlA [H]

Synonym: Bpro_3066

Alternate gene names: 91788926

Gene position: 3232944-3231826 (Counterclockwise)

Preceding gene: 91788927

Following gene: 91788925

Centisome position: 62.17

GC content: 60.14

Gene sequence:

>1119_bases
ATGAGCATCACCACGACAAACCAGGCCAGCAAGAAAAGAATCGCAGTCATCGCCGGTGACGGCATCGGCAAGGAAACCAT
GCCCGAGGGCGTTCGCGTGCTGGATGCGGCGGCGCGCAAATTCGGCATCGACCTGCACTTTGACCACTTCGATTTTTCCA
GCTGGGACTATTACGAAAAGCACGGCCAGATGATGCCGGACAACTGGAAAGACCAGATTGGCGGGCATGACGCGATTTAC
TTTGGTGCGGTGGGCTGGCCCGAGAAAATTGCCGACCATGTATCGTTGTGGGGTTCGTTGCTCCTGTTCAGGCGCGAGTT
TGACCAATACATCAACCTGCGGCCGGCGCGGCTGATGCCCGGCATCATCGCACCGGTGGTACGGCGTGATGGCAGCCCGC
GGCAACCCGGCGAGATCGACTTTTACATCGTTCGTGAAAATACCGAGGGCGAGTACTCCAGCATCGGCGGCAGGGCGTTT
CCCGGCACCGATCGCGAGTTTGTATTGCAGGAGTCGGTTTTTACGCGGGTCGGCGTGGATCGCGTTCTGAAGTTTGCGTT
TGAGCTGGCCCAGTCGCGCCCCAAGAAACACCTGACCAGCGCGACCAAGTCCAACGGCATTTCAATCTCCATGCCTTACT
GGGATGAACGGGTGGCCGAGATGGCCAAGAGCTATCCGGCGATTCGGCTGGACAAGTTTCACATTGACATCCTGACCGCG
CATTTTGTGCAGCGCCCGGACTTTTTCGATGTGGTGGTGGCCAGCAATCTGTTCGGCGACATCCTGAGCGACCTCGGACC
TGCCTGCACCGGCACGATCGGTATTGCACCGAGTGCCAACCTGAATCCGGAGCGCAAGTTCCCTTCGCTGTTCGAGCCGG
TGCATGGCTCGGCGCCGGACATCGCAGGGCGCAACCTTGCCAATCCCATTGGCCAGATCTGGTGCGGCGCCATGATGATG
GAGTTCCTGGGGCACAAGGAGGCGCATGATGCCATTCTTGCCGCGATTGAGAAAGTGCTGCGCCCCGACAGCGGTGCACC
GCGCACCCCGGACCTGGGTGGCAAGGCCGGTACGGCCGATGTTGGCAAGGCTGTGGCGGCTGCGCTGGCAGAGGTGTAA

Upstream 100 bases:

>100_bases
ATAAGCCTGCCGTGCGGGCACTATATTGCGGAAGAAGCGCCAGCATTGTTGCTGGAACAAGCCCTGAATTTTTTCGAAAC
TTAAACCAGAGGACCACATC

Downstream 100 bases:

>100_bases
CGGTATTTTGTCAAGCGCGAAAATTACGCGTTGCACTTATGATTAACCGATTAAAGCGGGAAAAAACCGACTAGAATCCG
CCTCCGCACGTTGTAAAAAC

Product: tartrate dehydrogenase

Products: NA

Alternate protein names: D-malate degradation protein A; D-malate oxidase [H]

Number of amino acids: Translated: 372; Mature: 371

Protein sequence:

>372_residues
MSITTTNQASKKRIAVIAGDGIGKETMPEGVRVLDAAARKFGIDLHFDHFDFSSWDYYEKHGQMMPDNWKDQIGGHDAIY
FGAVGWPEKIADHVSLWGSLLLFRREFDQYINLRPARLMPGIIAPVVRRDGSPRQPGEIDFYIVRENTEGEYSSIGGRAF
PGTDREFVLQESVFTRVGVDRVLKFAFELAQSRPKKHLTSATKSNGISISMPYWDERVAEMAKSYPAIRLDKFHIDILTA
HFVQRPDFFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERKFPSLFEPVHGSAPDIAGRNLANPIGQIWCGAMMM
EFLGHKEAHDAILAAIEKVLRPDSGAPRTPDLGGKAGTADVGKAVAAALAEV

Sequences:

>Translated_372_residues
MSITTTNQASKKRIAVIAGDGIGKETMPEGVRVLDAAARKFGIDLHFDHFDFSSWDYYEKHGQMMPDNWKDQIGGHDAIY
FGAVGWPEKIADHVSLWGSLLLFRREFDQYINLRPARLMPGIIAPVVRRDGSPRQPGEIDFYIVRENTEGEYSSIGGRAF
PGTDREFVLQESVFTRVGVDRVLKFAFELAQSRPKKHLTSATKSNGISISMPYWDERVAEMAKSYPAIRLDKFHIDILTA
HFVQRPDFFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERKFPSLFEPVHGSAPDIAGRNLANPIGQIWCGAMMM
EFLGHKEAHDAILAAIEKVLRPDSGAPRTPDLGGKAGTADVGKAVAAALAEV
>Mature_371_residues
SITTTNQASKKRIAVIAGDGIGKETMPEGVRVLDAAARKFGIDLHFDHFDFSSWDYYEKHGQMMPDNWKDQIGGHDAIYF
GAVGWPEKIADHVSLWGSLLLFRREFDQYINLRPARLMPGIIAPVVRRDGSPRQPGEIDFYIVRENTEGEYSSIGGRAFP
GTDREFVLQESVFTRVGVDRVLKFAFELAQSRPKKHLTSATKSNGISISMPYWDERVAEMAKSYPAIRLDKFHIDILTAH
FVQRPDFFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERKFPSLFEPVHGSAPDIAGRNLANPIGQIWCGAMMME
FLGHKEAHDAILAAIEKVLRPDSGAPRTPDLGGKAGTADVGKAVAAALAEV

Specific function: Catalyzes the NAD(+)-dependent oxidative decarboxylation of D-malate into pyruvate. Is essential for aerobic growth on D- malate as the sole carbon source. But is not required for anaerobic D-malate utilization, although DmlA is expressed and active in th

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=362, Percent_Identity=29.5580110497238, Blast_Score=151, Evalue=9e-37,
Organism=Homo sapiens, GI4758582, Length=260, Percent_Identity=28.4615384615385, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI28178816, Length=359, Percent_Identity=26.7409470752089, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI28178821, Length=359, Percent_Identity=26.1838440111421, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI28178838, Length=223, Percent_Identity=29.1479820627803, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI28178819, Length=199, Percent_Identity=30.1507537688442, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI1788101, Length=359, Percent_Identity=66.5738161559889, Blast_Score=499, Evalue=1e-142,
Organism=Escherichia coli, GI87081683, Length=365, Percent_Identity=37.5342465753425, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI1787381, Length=372, Percent_Identity=28.2258064516129, Blast_Score=98, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17550882, Length=364, Percent_Identity=29.9450549450549, Blast_Score=148, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI71986051, Length=374, Percent_Identity=29.4117647058824, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI25144293, Length=360, Percent_Identity=26.1111111111111, Blast_Score=111, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17505779, Length=262, Percent_Identity=27.8625954198473, Blast_Score=108, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6322097, Length=378, Percent_Identity=35.978835978836, Blast_Score=193, Evalue=3e-50,
Organism=Saccharomyces cerevisiae, GI6319830, Length=379, Percent_Identity=32.4538258575198, Blast_Score=137, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6324709, Length=364, Percent_Identity=31.043956043956, Blast_Score=134, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6324291, Length=361, Percent_Identity=27.9778393351801, Blast_Score=123, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24643268, Length=360, Percent_Identity=30.8333333333333, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24643270, Length=360, Percent_Identity=30.8333333333333, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24661184, Length=362, Percent_Identity=30.6629834254144, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI161078639, Length=269, Percent_Identity=30.4832713754647, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI161078637, Length=269, Percent_Identity=30.4832713754647, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI161078633, Length=269, Percent_Identity=30.4832713754647, Blast_Score=128, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24650122, Length=269, Percent_Identity=30.4832713754647, Blast_Score=128, Evalue=5e-30,
Organism=Drosophila melanogaster, GI161078635, Length=269, Percent_Identity=30.4832713754647, Blast_Score=128, Evalue=6e-30,
Organism=Drosophila melanogaster, GI281362242, Length=363, Percent_Identity=27.2727272727273, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24648872, Length=363, Percent_Identity=27.2727272727273, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI20130355, Length=371, Percent_Identity=25.0673854447439, Blast_Score=107, Evalue=9e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011829 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.83 [H]

Molecular weight: Translated: 40883; Mature: 40752

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSITTTNQASKKRIAVIAGDGIGKETMPEGVRVLDAAARKFGIDLHFDHFDFSSWDYYEK
CCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHH
HGQMMPDNWKDQIGGHDAIYFGAVGWPEKIADHVSLWGSLLLFRREFDQYINLRPARLMP
CCCCCCCCHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCC
GIIAPVVRRDGSPRQPGEIDFYIVRENTEGEYSSIGGRAFPGTDREFVLQESVFTRVGVD
HHHHHHHHCCCCCCCCCCEEEEEEEECCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
RVLKFAFELAQSRPKKHLTSATKSNGISISMPYWDERVAEMAKSYPAIRLDKFHIDILTA
HHHHHHHHHHHCCCHHHHHHHHCCCCEEEECCCHHHHHHHHHHCCCCEEEEHHHHHHHHH
HFVQRPDFFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERKFPSLFEPVHGSAPD
HHHCCCCHHHHHHHHHHHHHHHHHCCCHHCCCEECCCCCCCCCCCCCCHHHHCCCCCCCC
IAGRNLANPIGQIWCGAMMMEFLGHKEAHDAILAAIEKVLRPDSGAPRTPDLGGKAGTAD
CCCCHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHH
VGKAVAAALAEV
HHHHHHHHHHCC
>Mature Secondary Structure 
SITTTNQASKKRIAVIAGDGIGKETMPEGVRVLDAAARKFGIDLHFDHFDFSSWDYYEK
CCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHH
HGQMMPDNWKDQIGGHDAIYFGAVGWPEKIADHVSLWGSLLLFRREFDQYINLRPARLMP
CCCCCCCCHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCC
GIIAPVVRRDGSPRQPGEIDFYIVRENTEGEYSSIGGRAFPGTDREFVLQESVFTRVGVD
HHHHHHHHCCCCCCCCCCEEEEEEEECCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
RVLKFAFELAQSRPKKHLTSATKSNGISISMPYWDERVAEMAKSYPAIRLDKFHIDILTA
HHHHHHHHHHHCCCHHHHHHHHCCCCEEEECCCHHHHHHHHHHCCCCEEEEHHHHHHHHH
HFVQRPDFFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERKFPSLFEPVHGSAPD
HHHCCCCHHHHHHHHHHHHHHHHHCCCHHCCCEECCCCCCCCCCCCCCHHHHCCCCCCCC
IAGRNLANPIGQIWCGAMMMEFLGHKEAHDAILAAIEKVLRPDSGAPRTPDLGGKAGTAD
CCCCHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHH
VGKAVAAALAEV
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]