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The map label for this gene is pfkB [H]

Identifier: 91788618

GI number: 91788618

Start: 2899017

End: 2899955

Strand: Direct

Name: pfkB [H]

Synonym: Bpro_2756

Alternate gene names: 91788618

Gene position: 2899017-2899955 (Clockwise)

Preceding gene: 91788617

Following gene: 91788619

Centisome position: 55.75

GC content: 68.26

Gene sequence:

>939_bases
ATGACCGACATCCTGACCATCACCATGAACCCGGCCCTGGACGTCTCCACCTCGACCGACAAGGTCATGGACACGCACAA
GCTGCGGTGCGCTGCCGCGCAATTGCATCCTGGCGGCGGCGGGATCAACGTGGCTCGCGTCGTGCAGCGGCTCGGCAGCG
ACTGCATTGCGCTCTTTCTGGCTGGGGGCATAAACGGGCAACTGCTGCGGCAACTGCTGGACCGGGAGCAGGTGCGCAGC
CACTGGATCCCCATTGCCGGGGAAACCCGTGAGAGCTTTTCCGTCCACGAAACATCCAGCGGGAAGGACTTCCGCTTTGT
ACTGCCCGGCCCGACCCTGACGGCGCCGGAGTGGCAGGCCTGCCTGGACTTTGTCAGCAAGCTCGGCGTGCCTGCCGCTT
ATGTGGTGGCGAGCGGCGGCCTGCCCCCGGGGGTACCTGGCGACTTTTATGCTCAACTCACGCGGCTGACCCGCTCGCGC
GGCAGCCGGGTGGTGGTGGACACGTGGGGGCCGGCGCTGGCGGCGGCCCTGGCAGAAGGGGTGTACCTGATCAAGCCCAG
CCTTCAGGAACTGCGCGAGTTGACCGGCTTGCCGCTGGCTGACGAACCGCAATGGCGCGAGGCGGCAAAGCGGATCATCC
TGGCGGGGCACGCGCAGGTCGTGGCGCTGTCGCTCGGCGAGGACGGCGCCCTGCTCGTCACCGCAGACCGGACTCTGCGG
GCCCGGGCAGTACCGGTCAAGGTGGCCAGCACCATCGGGGCGGGTGACAGCTTTGTCGGCGGCCTGGTCTGGGCGCTGAA
CCGCGGCGACGACCTGGAGCAGGCGTTTCGTTACGGCATGGCGTCCGGCGCGGCGGCCCTGCTCACTGCGGGAACGGCCC
TGGGCCAGGCGGCCGATGTTGAGCGCCTTAGCAAGGACGTGATCGTCGAACCAGCCTGA

Upstream 100 bases:

>100_bases
GATCAAGGATGGTGCGCAGCGCGAGGCGATTCATGTCGCCGTGCTGCTGGCGCGGCAGCTGCGGGTCTGAGGCAGGGAAA
CACGGGAGCAAGTGAAGGCC

Downstream 100 bases:

>100_bases
GCCTGGTCTTGCTGAGCCTGGTCTTGCTGAGCCTGGTCTTGCGTCCCTGGAAATAACAGCATCAAAAGTAGCGAACCCAG
GGGCCCGTCTTTGATCTGGC

Product: 6-phosphofructokinase

Products: ADP; D-Fructose 1,6-bisphosphate; H+ [C]

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MTDILTITMNPALDVSTSTDKVMDTHKLRCAAAQLHPGGGGINVARVVQRLGSDCIALFLAGGINGQLLRQLLDREQVRS
HWIPIAGETRESFSVHETSSGKDFRFVLPGPTLTAPEWQACLDFVSKLGVPAAYVVASGGLPPGVPGDFYAQLTRLTRSR
GSRVVVDTWGPALAAALAEGVYLIKPSLQELRELTGLPLADEPQWREAAKRIILAGHAQVVALSLGEDGALLVTADRTLR
ARAVPVKVASTIGAGDSFVGGLVWALNRGDDLEQAFRYGMASGAAALLTAGTALGQAADVERLSKDVIVEPA

Sequences:

>Translated_312_residues
MTDILTITMNPALDVSTSTDKVMDTHKLRCAAAQLHPGGGGINVARVVQRLGSDCIALFLAGGINGQLLRQLLDREQVRS
HWIPIAGETRESFSVHETSSGKDFRFVLPGPTLTAPEWQACLDFVSKLGVPAAYVVASGGLPPGVPGDFYAQLTRLTRSR
GSRVVVDTWGPALAAALAEGVYLIKPSLQELRELTGLPLADEPQWREAAKRIILAGHAQVVALSLGEDGALLVTADRTLR
ARAVPVKVASTIGAGDSFVGGLVWALNRGDDLEQAFRYGMASGAAALLTAGTALGQAADVERLSKDVIVEPA
>Mature_311_residues
TDILTITMNPALDVSTSTDKVMDTHKLRCAAAQLHPGGGGINVARVVQRLGSDCIALFLAGGINGQLLRQLLDREQVRSH
WIPIAGETRESFSVHETSSGKDFRFVLPGPTLTAPEWQACLDFVSKLGVPAAYVVASGGLPPGVPGDFYAQLTRLTRSRG
SRVVVDTWGPALAAALAEGVYLIKPSLQELRELTGLPLADEPQWREAAKRIILAGHAQVVALSLGEDGALLVTADRTLRA
RAVPVKVASTIGAGDSFVGGLVWALNRGDDLEQAFRYGMASGAAALLTAGTALGQAADVERLSKDVIVEPA

Specific function: Key control step of glycolysis. [C]

COG id: COG1105

COG function: function code G; Fructose-1-phosphate kinase and related fructose-6-phosphate kinase (PfkB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Escherichia coli, GI1788017, Length=304, Percent_Identity=39.8026315789474, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1788493, Length=276, Percent_Identity=34.0579710144928, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: 496 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR017583 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: 2.7.1.11 [C]

Molecular weight: Translated: 32878; Mature: 32747

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00583 PFKB_KINASES_1 ; PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDILTITMNPALDVSTSTDKVMDTHKLRCAAAQLHPGGGGINVARVVQRLGSDCIALFL
CCEEEEEEECCCEECCCCCHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHCHHHHHHHH
AGGINGQLLRQLLDREQVRSHWIPIAGETRESFSVHETSSGKDFRFVLPGPTLTAPEWQA
HCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCEEEEECCCCCCCCCHHH
CLDFVSKLGVPAAYVVASGGLPPGVPGDFYAQLTRLTRSRGSRVVVDTWGPALAAALAEG
HHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHCC
VYLIKPSLQELRELTGLPLADEPQWREAAKRIILAGHAQVVALSLGEDGALLVTADRTLR
EEEECCCHHHHHHHHCCCCCCCCHHHHHHHHEEEECCCEEEEEEECCCCEEEEEECCCEE
ARAVPVKVASTIGAGDSFVGGLVWALNRGDDLEQAFRYGMASGAAALLTAGTALGQAADV
EEECEEEEHHHCCCCCHHHHHHHEEECCCCCHHHHHHHCCCCCHHHHHHHHHHHCCHHHH
ERLSKDVIVEPA
HHHHHCCEECCC
>Mature Secondary Structure 
TDILTITMNPALDVSTSTDKVMDTHKLRCAAAQLHPGGGGINVARVVQRLGSDCIALFL
CEEEEEEECCCEECCCCCHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHCHHHHHHHH
AGGINGQLLRQLLDREQVRSHWIPIAGETRESFSVHETSSGKDFRFVLPGPTLTAPEWQA
HCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCEEEEECCCCCCCCCHHH
CLDFVSKLGVPAAYVVASGGLPPGVPGDFYAQLTRLTRSRGSRVVVDTWGPALAAALAEG
HHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHCC
VYLIKPSLQELRELTGLPLADEPQWREAAKRIILAGHAQVVALSLGEDGALLVTADRTLR
EEEECCCHHHHHHHHCCCCCCCCHHHHHHHHEEEECCCEEEEEEECCCCEEEEEECCCEE
ARAVPVKVASTIGAGDSFVGGLVWALNRGDDLEQAFRYGMASGAAALLTAGTALGQAADV
EEECEEEEHHHCCCCCHHHHHHHEEECCCCCHHHHHHHCCCCCHHHHHHHHHHHCCHHHH
ERLSKDVIVEPA
HHHHHCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Co2+; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; D-Fructose 6-phosphate [C]

Specific reaction: ATP + D-Fructose 6-phosphate --> ADP + D-Fructose 1,6-bisphosphate + H+ [C]

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]