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The map label for this gene is ygfZ [C]

Identifier: 91788384

GI number: 91788384

Start: 2635262

End: 2636215

Strand: Direct

Name: ygfZ [C]

Synonym: Bpro_2521

Alternate gene names: 91788384

Gene position: 2635262-2636215 (Clockwise)

Preceding gene: 91788375

Following gene: 91788385

Centisome position: 50.68

GC content: 64.05

Gene sequence:

>954_bases
ATGACCCTGTCCACAACTGACGCCGAAATTTCCGCCCCTGTCTCGGGCGTGGCCGAATTGGCTCACCTGGGCGTGATTCG
TGTGGCGGGCGAAGATACCGTGAAGTTCCTGCAGGGCCAGCTGACGCAGGACGTGGCCCTGCTGAGCCTCTCAGAGGCCC
GACTGGGCGCCTTTTGCAATGTGAAAGGCCGCATGCAGGCCAGCTTTGTGATTTTCAAGCGCAGCCCCGAGGAGGTTCTG
CTGGTGTGCAGCCGCGACATTTTGCCGGCCACACTCAAGCGCCTGTCGATGTTCGTGCTGCGCGCCAAGGCCATGCTCAG
CGATGCCAGCGCCGAGTTCGCCTTGTACGGCGTGGCGGGAAATGCCATTGAATTGATCGTTGGCGGCAACCGCCCTGTCT
GGACAAAGTCCGACATTGGCGATGCAAGCCTGATGTTTTTACACCCCGGTGCCGGCCAGCCGCGCGCGCTCTGGTGCGCA
CCCGCCGGCTCGCCCCGCCCCGAGGGCCCGCTCCTGGACATTGCACGCTGGAACTGGCTGGAGGTGCGGTCCGGCATTGC
CATGATCACCCAGCCGATCTTTGAGGCCTTTGTGCCGCAAATGCTCAATTACGAATCCGTGGGCGGCGTGAACTTCAAGA
AGGGCTGCTACCCCGGGCAGGAGATCGTGGCACGCAGCCAGTTTCGCGGCACGCTGAAACGGCGGGCCTACCTGGCACAT
ACCGCGGGCGTGCCGGCAGTGGGACAAGAGGTGTTTCATGCCTCCGACGCCGAGCAACCTTGCGGCCTGGTGGCCGCCGC
CGCAGCCACCCCTTCGGGCGGTTTTGATGCCATTGTGTCGATGCAAACGTCGGCCGCCGCCGATGCGGGCGAGGGACGCC
TCACCTTGGGAAGCGCCACGGGTACGCCGCTGGCATTGCTGCCACTGCCCTATCCCCTGCTTGAAGATATCTGA

Upstream 100 bases:

>100_bases
AAAGACACGACCCACAACCCGGCTACCTTTTCGACACTGGAACCCAAGAACACAGGGCTATGATAATTCAGGCCATCCTT
ACAACTACTGCGAACCGACC

Downstream 100 bases:

>100_bases
GCGAACGCCCAAAATCTGCAAGAAAGTCCGAAAGCTTTACCGTGTGCCTGATCGCATTTGCCATCAATGCCTCTGAACGT
TGGCCGCTGGTGATTGCGGC

Product: glycine cleavage T protein (aminomethyl transferase)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MTLSTTDAEISAPVSGVAELAHLGVIRVAGEDTVKFLQGQLTQDVALLSLSEARLGAFCNVKGRMQASFVIFKRSPEEVL
LVCSRDILPATLKRLSMFVLRAKAMLSDASAEFALYGVAGNAIELIVGGNRPVWTKSDIGDASLMFLHPGAGQPRALWCA
PAGSPRPEGPLLDIARWNWLEVRSGIAMITQPIFEAFVPQMLNYESVGGVNFKKGCYPGQEIVARSQFRGTLKRRAYLAH
TAGVPAVGQEVFHASDAEQPCGLVAAAAATPSGGFDAIVSMQTSAAADAGEGRLTLGSATGTPLALLPLPYPLLEDI

Sequences:

>Translated_317_residues
MTLSTTDAEISAPVSGVAELAHLGVIRVAGEDTVKFLQGQLTQDVALLSLSEARLGAFCNVKGRMQASFVIFKRSPEEVL
LVCSRDILPATLKRLSMFVLRAKAMLSDASAEFALYGVAGNAIELIVGGNRPVWTKSDIGDASLMFLHPGAGQPRALWCA
PAGSPRPEGPLLDIARWNWLEVRSGIAMITQPIFEAFVPQMLNYESVGGVNFKKGCYPGQEIVARSQFRGTLKRRAYLAH
TAGVPAVGQEVFHASDAEQPCGLVAAAAATPSGGFDAIVSMQTSAAADAGEGRLTLGSATGTPLALLPLPYPLLEDI
>Mature_316_residues
TLSTTDAEISAPVSGVAELAHLGVIRVAGEDTVKFLQGQLTQDVALLSLSEARLGAFCNVKGRMQASFVIFKRSPEEVLL
VCSRDILPATLKRLSMFVLRAKAMLSDASAEFALYGVAGNAIELIVGGNRPVWTKSDIGDASLMFLHPGAGQPRALWCAP
AGSPRPEGPLLDIARWNWLEVRSGIAMITQPIFEAFVPQMLNYESVGGVNFKKGCYPGQEIVARSQFRGTLKRRAYLAHT
AGVPAVGQEVFHASDAEQPCGLVAAAAATPSGGFDAIVSMQTSAAADAGEGRLTLGSATGTPLALLPLPYPLLEDI

Specific function: Folate-binding protein involved in regulating the level of ATP-dnaA and in the modification of some tRNAs. It is probably a key factor in regulatory networks that act via tRNA modification, such as initiation of chromosomal replication [H]

COG id: COG0354

COG function: function code R; Predicted aminomethyltransferase related to GcvT

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA-modifying ygfZ family [H]

Homologues:

Organism=Escherichia coli, GI1789265, Length=304, Percent_Identity=27.3026315789474, Blast_Score=99, Evalue=3e-22,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017703
- InterPro:   IPR006222 [H]

Pfam domain/function: PF01571 GCV_T [H]

EC number: NA

Molecular weight: Translated: 33464; Mature: 33333

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLSTTDAEISAPVSGVAELAHLGVIRVAGEDTVKFLQGQLTQDVALLSLSEARLGAFCN
CCCCCCCCCCCCCHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHEEECHHHHCEEEC
VKGRMQASFVIFKRSPEEVLLVCSRDILPATLKRLSMFVLRAKAMLSDASAEFALYGVAG
CCCCEEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECC
NAIELIVGGNRPVWTKSDIGDASLMFLHPGAGQPRALWCAPAGSPRPEGPLLDIARWNWL
CEEEEEECCCCCEECCCCCCCEEEEEEECCCCCCCEEEEECCCCCCCCCCEEEECCCCHH
EVRSGIAMITQPIFEAFVPQMLNYESVGGVNFKKGCYPGQEIVARSQFRGTLKRRAYLAH
HHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
TAGVPAVGQEVFHASDAEQPCGLVAAAAATPSGGFDAIVSMQTSAAADAGEGRLTLGSAT
CCCCCHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCEEEEECHHHCCCCCCCEEEEECCC
GTPLALLPLPYPLLEDI
CCCEEEECCCCHHHHCC
>Mature Secondary Structure 
TLSTTDAEISAPVSGVAELAHLGVIRVAGEDTVKFLQGQLTQDVALLSLSEARLGAFCN
CCCCCCCCCCCCHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHEEECHHHHCEEEC
VKGRMQASFVIFKRSPEEVLLVCSRDILPATLKRLSMFVLRAKAMLSDASAEFALYGVAG
CCCCEEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECC
NAIELIVGGNRPVWTKSDIGDASLMFLHPGAGQPRALWCAPAGSPRPEGPLLDIARWNWL
CEEEEEECCCCCEECCCCCCCEEEEEEECCCCCCCEEEEECCCCCCCCCCEEEECCCCHH
EVRSGIAMITQPIFEAFVPQMLNYESVGGVNFKKGCYPGQEIVARSQFRGTLKRRAYLAH
HHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
TAGVPAVGQEVFHASDAEQPCGLVAAAAATPSGGFDAIVSMQTSAAADAGEGRLTLGSAT
CCCCCHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCEEEEECHHHCCCCCCCEEEEECCC
GTPLALLPLPYPLLEDI
CCCEEEECCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA