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The map label for this gene is xthA [H]

Identifier: 91787699

GI number: 91787699

Start: 1885339

End: 1886142

Strand: Reverse

Name: xthA [H]

Synonym: Bpro_1820

Alternate gene names: 91787699

Gene position: 1886142-1885339 (Counterclockwise)

Preceding gene: 91787700

Following gene: 91787694

Centisome position: 36.27

GC content: 59.7

Gene sequence:

>804_bases
TTGCCCTGGAACACCAAAACCATGAAAATCGCCACCTGGAATGTCAACTCGCTGGCCGTGCGGCTGCCGCAGGTCCTTGA
CTGGCTGGCGGCAAATCCGGTGGATGTGCTGTGCCTGCAGGAACTCAAACTTTCCGACGACAAGTTTCCACTGCAGGCAC
TGGCCGACGCGGGCTACCAAAGTGCGGTGTTCGGCCAGAAAACCTACAACGGCGTGGCCATTTTGAGTCGCACGCCGGCG
CGCAACATTGTGAAGAACATAGCCGGTTTCACGGACGAGCAGTCACGCGTGATCGCGGCGACGCTGGACACGCCGAGCGG
CGAGGTGCGCGTTGTCAATGGCTATTTTGTCAACGGCCAGGCACCGGGCACCGAGAAGTTTGAATACAAGATGCGCTGGC
TCGACGGCCTGCGCGCGTGGCTGCGCGAAGAACTCGCCGAACACTCCAGGCTTGTGCTGGTGGGTGACTTCAACATCACC
GCCGACGACCGCGACACGTATGACGCGCAAGGGCTGCACGAAACGATTCACCATACCGCCGACGAACGGCAACACTTGCA
GGCACTGTTGGGGCTGGGTCTGCACGATGCGTTCCGCCTGTTCGAACAACCGGAAAAAACCTACTCATGGTGGGATTACC
GGGAGTTTGCGTTTCGCAGAAACCGCGGCCTGCGCATTGATTACATCCTGGTGAGCAATGCACTCAAGCCCGCCGTCAAA
AGCTGCCTCATCGACAAGCTGCCACGCAAAAACGAGCGCCCGAGCGACCACGCGCCGGTCGTGGCGGAACTCGATTGGCT
ATAA

Upstream 100 bases:

>100_bases
CGTTGGCTGGAATATGGCATGTTCGCGCTGGCCGGCGTGGGCATGGCGGTCGGCGCGCTCGCGTTGCTGCATATTTGATA
GCATCTCCTCCTTATCTTGC

Downstream 100 bases:

>100_bases
AAACAATAGCAATACTGTCTCATTCCACGATAGGTAGAGCCACTATTTACTAAAATCCAGGCCCTGTCCCCTGCACGGGG
TCGGCGCGCGTCCGGCTCCG

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MPWNTKTMKIATWNVNSLAVRLPQVLDWLAANPVDVLCLQELKLSDDKFPLQALADAGYQSAVFGQKTYNGVAILSRTPA
RNIVKNIAGFTDEQSRVIAATLDTPSGEVRVVNGYFVNGQAPGTEKFEYKMRWLDGLRAWLREELAEHSRLVLVGDFNIT
ADDRDTYDAQGLHETIHHTADERQHLQALLGLGLHDAFRLFEQPEKTYSWWDYREFAFRRNRGLRIDYILVSNALKPAVK
SCLIDKLPRKNERPSDHAPVVAELDWL

Sequences:

>Translated_267_residues
MPWNTKTMKIATWNVNSLAVRLPQVLDWLAANPVDVLCLQELKLSDDKFPLQALADAGYQSAVFGQKTYNGVAILSRTPA
RNIVKNIAGFTDEQSRVIAATLDTPSGEVRVVNGYFVNGQAPGTEKFEYKMRWLDGLRAWLREELAEHSRLVLVGDFNIT
ADDRDTYDAQGLHETIHHTADERQHLQALLGLGLHDAFRLFEQPEKTYSWWDYREFAFRRNRGLRIDYILVSNALKPAVK
SCLIDKLPRKNERPSDHAPVVAELDWL
>Mature_266_residues
PWNTKTMKIATWNVNSLAVRLPQVLDWLAANPVDVLCLQELKLSDDKFPLQALADAGYQSAVFGQKTYNGVAILSRTPAR
NIVKNIAGFTDEQSRVIAATLDTPSGEVRVVNGYFVNGQAPGTEKFEYKMRWLDGLRAWLREELAEHSRLVLVGDFNITA
DDRDTYDAQGLHETIHHTADERQHLQALLGLGLHDAFRLFEQPEKTYSWWDYREFAFRRNRGLRIDYILVSNALKPAVKS
CLIDKLPRKNERPSDHAPVVAELDWL

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=263, Percent_Identity=34.6007604562738, Blast_Score=125, Evalue=3e-29,
Organism=Homo sapiens, GI18375503, Length=263, Percent_Identity=34.6007604562738, Blast_Score=125, Evalue=3e-29,
Organism=Homo sapiens, GI18375501, Length=263, Percent_Identity=34.6007604562738, Blast_Score=125, Evalue=3e-29,
Organism=Escherichia coli, GI1788046, Length=264, Percent_Identity=35.2272727272727, Blast_Score=141, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI71989536, Length=274, Percent_Identity=27.007299270073, Blast_Score=77, Evalue=7e-15,
Organism=Drosophila melanogaster, GI221330655, Length=264, Percent_Identity=29.1666666666667, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI17136678, Length=270, Percent_Identity=28.5185185185185, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 30432; Mature: 30301

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPWNTKTMKIATWNVNSLAVRLPQVLDWLAANPVDVLCLQELKLSDDKFPLQALADAGYQ
CCCCCCEEEEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHCCCH
SAVFGQKTYNGVAILSRTPARNIVKNIAGFTDEQSRVIAATLDTPSGEVRVVNGYFVNGQ
HHHCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCCEEEEEEECCCCCCEEEEEEEEECCC
APGTEKFEYKMRWLDGLRAWLREELAEHSRLVLVGDFNITADDRDTYDAQGLHETIHHTA
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEECCCCCCCCHHHHHHHHHHHH
DERQHLQALLGLGLHDAFRLFEQPEKTYSWWDYREFAFRRNRGLRIDYILVSNALKPAVK
HHHHHHHHHHCCCHHHHHHHHHCCCHHCCHHHHHHHHHHCCCCCEEEEEEECHHHHHHHH
SCLIDKLPRKNERPSDHAPVVAELDWL
HHHHHHCCCCCCCCCCCCCEEEEECCC
>Mature Secondary Structure 
PWNTKTMKIATWNVNSLAVRLPQVLDWLAANPVDVLCLQELKLSDDKFPLQALADAGYQ
CCCCCEEEEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHCCCH
SAVFGQKTYNGVAILSRTPARNIVKNIAGFTDEQSRVIAATLDTPSGEVRVVNGYFVNGQ
HHHCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCCEEEEEEECCCCCCEEEEEEEEECCC
APGTEKFEYKMRWLDGLRAWLREELAEHSRLVLVGDFNITADDRDTYDAQGLHETIHHTA
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEECCCCCCCCHHHHHHHHHHHH
DERQHLQALLGLGLHDAFRLFEQPEKTYSWWDYREFAFRRNRGLRIDYILVSNALKPAVK
HHHHHHHHHHCCCHHHHHHHHHCCCHHCCHHHHHHHHHHCCCCCEEEEEEECHHHHHHHH
SCLIDKLPRKNERPSDHAPVVAELDWL
HHHHHHCCCCCCCCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]