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The map label for this gene is trmD

Identifier: 91787574

GI number: 91787574

Start: 1742546

End: 1743325

Strand: Direct

Name: trmD

Synonym: Bpro_1692

Alternate gene names: 91787574

Gene position: 1742546-1743325 (Clockwise)

Preceding gene: 91787573

Following gene: 91787575

Centisome position: 33.51

GC content: 64.1

Gene sequence:

>780_bases
ATGCGTTTTGACGTCATCACCCTTTTCCCTGAGCTTTTTGGGCCATTTCTGGTCAGTGGCGTCACCCGCCGTGCCTACGA
AACAGGCCTGGTCGAGGTGAAGCTCTGGAATCCGCGCGATTTTGCCGAGGGCAGCTACCGCCGTGTGGACGACCGGCCGT
TTGGCGGCGGACCCGGCATGGTGATGATGGCTGAGCCGCTCACACTCTGCCTCGAACAAATACGTGCCGAGCGTACCGCT
GGCGGCGTGGCAGATGTGCCCACCGTGCTGTTCTCCCCCATAGGCGAGGCGCTGAACCATGCGGGCGTCGAAGCCTGGTC
AGCCAGCAGCGGTGCCGTGCTGGTTTGCGGCCGCTATGAGGGCCTGGACCAGCGTTTCATCGAAACCCATATCGATCGCC
AGATCAGCCTGGGCGATTTCGTGTTGTCCGGCGGCGAGATCGCCGCCATGGCCCTGCTGGACGCCGTCGCCCGTTTGCAG
CCCGGCGTGCTGAACGACGAAGGCAGCCACCAGCTGGACAGCTTCAATCCGGCGCTGGATGGCTTGCTGGATTGCCCGCA
CTACACCCGTCCGGAATCCTGGCGCGGCCAGCCTGTGCCGCCCATCCTGTTGTCCGGCAACCATGCCCAGATCGAGCGCT
GGCGGCGCGAAGAGCGCCTGGCGCTGACCCAGCGCCAGCGTCCCGAACTGGTGCAAAAGGCGCGGGCGGCCGGCCATTTG
ACTGCGCGCGACGAAGCTTTCCTGGCAGATTTGTTCGAAAATCCTGAACAAGATGCCTAG

Upstream 100 bases:

>100_bases
AATGCATCACGGTCGACTGGCAGCCCGACTATTGACGCCGGTGCAGGCTCTCGGGGGTGGATTGCCCTGAGCCGGCCGGT
GCGCGCAAAATAGCCTTTCC

Downstream 100 bases:

>100_bases
CTGCTATAATCAAAGGCTTTTCGATCCTCTGGCGGCCACTGCAACCATCTGAATCATCTGCGGATGAATCCATGGATTTG
CGGTCTTTAGCGTAGCGCGT

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MRFDVITLFPELFGPFLVSGVTRRAYETGLVEVKLWNPRDFAEGSYRRVDDRPFGGGPGMVMMAEPLTLCLEQIRAERTA
GGVADVPTVLFSPIGEALNHAGVEAWSASSGAVLVCGRYEGLDQRFIETHIDRQISLGDFVLSGGEIAAMALLDAVARLQ
PGVLNDEGSHQLDSFNPALDGLLDCPHYTRPESWRGQPVPPILLSGNHAQIERWRREERLALTQRQRPELVQKARAAGHL
TARDEAFLADLFENPEQDA

Sequences:

>Translated_259_residues
MRFDVITLFPELFGPFLVSGVTRRAYETGLVEVKLWNPRDFAEGSYRRVDDRPFGGGPGMVMMAEPLTLCLEQIRAERTA
GGVADVPTVLFSPIGEALNHAGVEAWSASSGAVLVCGRYEGLDQRFIETHIDRQISLGDFVLSGGEIAAMALLDAVARLQ
PGVLNDEGSHQLDSFNPALDGLLDCPHYTRPESWRGQPVPPILLSGNHAQIERWRREERLALTQRQRPELVQKARAAGHL
TARDEAFLADLFENPEQDA
>Mature_259_residues
MRFDVITLFPELFGPFLVSGVTRRAYETGLVEVKLWNPRDFAEGSYRRVDDRPFGGGPGMVMMAEPLTLCLEQIRAERTA
GGVADVPTVLFSPIGEALNHAGVEAWSASSGAVLVCGRYEGLDQRFIETHIDRQISLGDFVLSGGEIAAMALLDAVARLQ
PGVLNDEGSHQLDSFNPALDGLLDCPHYTRPESWRGQPVPPILLSGNHAQIERWRREERLALTQRQRPELVQKARAAGHL
TARDEAFLADLFENPEQDA

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=233, Percent_Identity=48.9270386266094, Blast_Score=226, Evalue=1e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_POLSJ (Q12CW4)

Other databases:

- EMBL:   CP000316
- RefSeq:   YP_548526.1
- ProteinModelPortal:   Q12CW4
- SMR:   Q12CW4
- STRING:   Q12CW4
- GeneID:   4015672
- GenomeReviews:   CP000316_GR
- KEGG:   pol:Bpro_1692
- NMPDR:   fig|296591.1.peg.2388
- eggNOG:   COG0336
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- PhylomeDB:   Q12CW4
- ProtClustDB:   PRK00026
- BioCyc:   PSP296591:BPRO_1692-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 28588; Mature: 28588

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00211 ABC_TRANSPORTER_1

Important sites: BINDING 117-117

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFDVITLFPELFGPFLVSGVTRRAYETGLVEVKLWNPRDFAEGSYRRVDDRPFGGGPGM
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCE
VMMAEPLTLCLEQIRAERTAGGVADVPTVLFSPIGEALNHAGVEAWSASSGAVLVCGRYE
EEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCC
GLDQRFIETHIDRQISLGDFVLSGGEIAAMALLDAVARLQPGVLNDEGSHQLDSFNPALD
CHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHH
GLLDCPHYTRPESWRGQPVPPILLSGNHAQIERWRREERLALTQRQRPELVQKARAAGHL
HHHCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCC
TARDEAFLADLFENPEQDA
CCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRFDVITLFPELFGPFLVSGVTRRAYETGLVEVKLWNPRDFAEGSYRRVDDRPFGGGPGM
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCE
VMMAEPLTLCLEQIRAERTAGGVADVPTVLFSPIGEALNHAGVEAWSASSGAVLVCGRYE
EEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCC
GLDQRFIETHIDRQISLGDFVLSGGEIAAMALLDAVARLQPGVLNDEGSHQLDSFNPALD
CHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHH
GLLDCPHYTRPESWRGQPVPPILLSGNHAQIERWRREERLALTQRQRPELVQKARAAGHL
HHHCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCC
TARDEAFLADLFENPEQDA
CCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA