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The map label for this gene is greB [H]

Identifier: 91787228

GI number: 91787228

Start: 1372470

End: 1373042

Strand: Direct

Name: greB [H]

Synonym: Bpro_1332

Alternate gene names: 91787228

Gene position: 1372470-1373042 (Clockwise)

Preceding gene: 91787227

Following gene: 91787233

Centisome position: 26.39

GC content: 58.99

Gene sequence:

>573_bases
ATGAGCAAAGCTTTCACCCGGGAAACCGAACAGGACGACGAAGACTTGGCGCTTGCGCCACTGCCTGCGGGCGGCAAGAA
TTACATCACGCCGGTGGGTTACGAGCGCCTGCGCGCCGAATTGCTCGACCTGATCGACAACGAACGTCCCAAGGTCGTGG
AGATTGTTCACTGGGCTGCCAGCAATGGTGACCGGTCTGAAAACGGGGACTATCACTACGGTAAAAAACGCCTGCGCGAG
ATTGACCGGCGCATCCGGTTTTTGACGCAAAGGCTGGAAATCGCCGAGATCAGCAACCCCTCCATCCACCATGGCAGCGA
CCAGGTGTTTTTTGGCGCCACCGTGACTTATGTCGATGACAGGGGCGAGGAGCGTACCGTCACCATTCTGGGGATTGATG
AAGCCAACAGCGCCTTGGGGCAAATCAGCTGGATTTCTCCGGCGGCCAGGGCGCTGCTCAAATCACGGGTGGGTGATGAA
GTGAACCTGGTCACGCCCCAGGGCCTGCAGGTGCTCGAAGTGCTGAAGGTGGACTATCCAGCGCCCGATGCCCGCGAGGA
CCTCAAGCAGTAA

Upstream 100 bases:

>100_bases
GAAAGCCTGCAACGAAAGTTGCAGGCTTTTTCGTTTTTTCGCCGGTCATTTTTGCTGTGGATCAGCAGCCTCAGCGCCTC
AGTGCTATTCTGGTCAGGCC

Downstream 100 bases:

>100_bases
AACGCAGAAACGTTACCCCTGCAATGTCACATCGAGGCCACAGAGCTACAGGTTAACGAACGTGGGGGCCAAGTGGCAGC
TCTCCAGCCCAGCAGGGGCC

Product: GreA/GreB family elongation factor

Products: NA

Alternate protein names: Transcript cleavage factor greB [H]

Number of amino acids: Translated: 190; Mature: 189

Protein sequence:

>190_residues
MSKAFTRETEQDDEDLALAPLPAGGKNYITPVGYERLRAELLDLIDNERPKVVEIVHWAASNGDRSENGDYHYGKKRLRE
IDRRIRFLTQRLEIAEISNPSIHHGSDQVFFGATVTYVDDRGEERTVTILGIDEANSALGQISWISPAARALLKSRVGDE
VNLVTPQGLQVLEVLKVDYPAPDAREDLKQ

Sequences:

>Translated_190_residues
MSKAFTRETEQDDEDLALAPLPAGGKNYITPVGYERLRAELLDLIDNERPKVVEIVHWAASNGDRSENGDYHYGKKRLRE
IDRRIRFLTQRLEIAEISNPSIHHGSDQVFFGATVTYVDDRGEERTVTILGIDEANSALGQISWISPAARALLKSRVGDE
VNLVTPQGLQVLEVLKVDYPAPDAREDLKQ
>Mature_189_residues
SKAFTRETEQDDEDLALAPLPAGGKNYITPVGYERLRAELLDLIDNERPKVVEIVHWAASNGDRSENGDYHYGKKRLREI
DRRIRFLTQRLEIAEISNPSIHHGSDQVFFGATVTYVDDRGEERTVTILGIDEANSALGQISWISPAARALLKSRVGDEV
NLVTPQGLQVLEVLKVDYPAPDAREDLKQ

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082261, Length=154, Percent_Identity=48.0519480519481, Blast_Score=120, Evalue=5e-29,
Organism=Escherichia coli, GI87082225, Length=152, Percent_Identity=35.5263157894737, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR001437
- InterPro:   IPR022691
- InterPro:   IPR006358 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 21324; Mature: 21193

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00829 GREAB_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKAFTRETEQDDEDLALAPLPAGGKNYITPVGYERLRAELLDLIDNERPKVVEIVHWAA
CCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECC
SNGDRSENGDYHYGKKRLREIDRRIRFLTQRLEIAEISNPSIHHGSDQVFFGATVTYVDD
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEECC
RGEERTVTILGIDEANSALGQISWISPAARALLKSRVGDEVNLVTPQGLQVLEVLKVDYP
CCCCEEEEEEEECCCCHHHHEEHHCCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHCCCC
APDAREDLKQ
CCCHHHHHCC
>Mature Secondary Structure 
SKAFTRETEQDDEDLALAPLPAGGKNYITPVGYERLRAELLDLIDNERPKVVEIVHWAA
CCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECC
SNGDRSENGDYHYGKKRLREIDRRIRFLTQRLEIAEISNPSIHHGSDQVFFGATVTYVDD
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEECC
RGEERTVTILGIDEANSALGQISWISPAARALLKSRVGDEVNLVTPQGLQVLEVLKVDYP
CCCCEEEEEEEECCCCHHHHEEHHCCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHCCCC
APDAREDLKQ
CCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]