Click here to switch to the map view.

The map label for this gene is pulE [H]

Identifier: 91787226

GI number: 91787226

Start: 1369461

End: 1370885

Strand: Direct

Name: pulE [H]

Synonym: Bpro_1330

Alternate gene names: 91787226

Gene position: 1369461-1370885 (Clockwise)

Preceding gene: 91787225

Following gene: 91787227

Centisome position: 26.33

GC content: 65.4

Gene sequence:

>1425_bases
ATGCGCTACCCATTGCCTTACGCCTTTGCCCGCAGCAATACCCTGCTGCTGGAAGACGATGGCCATGCGCTGACGCTGTG
GCATCCGGCCCGGCCCGACCCTGCGGCCCTGGGTGAAGTGATGCGCAAGTTCGCGGCGCACAGCCCCGCGCTGGTCCTTC
AGTGCGCCGACGCGGGAACCTTGACCCAGCGCATCAGCGCCGCCTACGCGGCAGGGGAGTCGAGTGCGGCCGCCGTGGTG
AGCGAGGTGGAGTCCGACGCCGACCTGTCGCGCATCATGCAGGAGTTGCCGGCGGTCGAGGATCTGCTGGAAACCTCCGA
TGACGCGCCCATCATCCGCATGCTCAATGCGCTGTTGACACAGGCCGCGCGCGACGGCGCGAGCGACATCCACATCGAGC
CTTACGAGCGGCATTCGAGCGTGCGTTTTCGCGTGGACGGCACCCTGCGCGAAGTCGTGCAGCCCAACCGGGCGCTGCAC
GCCGCGCTGATCTCGCGCCTGAAGATCATGGCGGACCTGGATATTTCGGAAAAACGCCTTCCGCAGGATGGCCGCATCAG
CCTGCGCATTGGCACGCGAGCGGTGGACGTGCGTGTGAGCACCTTGCCCAGCGCCCACGGTGAGCGCGCCGTGTTGCGCC
TGCTGGACAAGAGCGAGAGCAAGCTGAGCCTGGAGTCGGTCGGCATGCAGGGTGATGTGCTTCACCGTTTCGAACACCTG
ATCGCCCAGCCGCACGGCATCATCCTGGTCACCGGCCCCACGGGTTCGGGTAAAACCACCACCCTTTATGCTGCCTTGCA
GCGGCTCGATGCCAGCGCCAGCAACATCATGACGGTCGAAGATCCCATCGAGTACGAGCTTGCGGGCATCGGGCAGACGC
AGGTCAATTCAAAGATAGACCTGAGCTTTGCCAAGGCGCTGCGCGCCATCCTGCGCCAGGACCCGGACGTGATCATGATC
GGTGAGATCCGGGATTTTGAGACCGCGCAGATCGCCATACAGGCCTCGCTGACCGGCCACCTGGTGCTCGCCACGCTGCA
CACCAATGATGCGTCCAGTGCCGTGACGCGCCTGACCGATATCGGTGTGGAGCCCTTTTTGCTGAGTTCGTCCCTGCTCG
GCGTGCTGGCACAGCGGCTGGTGCGCAAGCGGTGCACCTACTGCCATGCTTCTTCGGCAGGTCCGGGCCTGGCCGCCGGC
TGCGAGCATTGCGGCTATACCGGTTACGCGGGCCGTACGGGCGTCTTTGAGTTGCTGGTGACCGATGACGCTGTTCGCGC
CCAGATTCACAACCGCGCGTCTGAGGCAGAAATTCGCGCAGCCGCGCTGCAGGCGGGCATGATGCTGATGCGCGACGACG
GTGAGCGGCTGGTCACCGCCGGCGTCACCTCGCGCGAGGAGTTGCTGCGCGTGACCCCCGACTGA

Upstream 100 bases:

>100_bases
CACCAGAAGCACCGCCGGCAGTGCCGACTGTCACCCCGCCGCCCGTCGCAACCCCGCTCGTGCCCGCCCCGGGCCAGTAA
ACCCGAACCAGTAAACCCCT

Downstream 100 bases:

>100_bases
ACCCGCACCGGCCCGAATACTGCGCCAAGCCATCATGCCCGCCTATTCTTTTGAGGCCATGCAGAACGACGGCAAGCTCC
GCCGGGGGGTGATCGAGGCT

Product: type II secretion system protein E

Products: NA

Alternate protein names: Pullulanase secretion protein pulE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MRYPLPYAFARSNTLLLEDDGHALTLWHPARPDPAALGEVMRKFAAHSPALVLQCADAGTLTQRISAAYAAGESSAAAVV
SEVESDADLSRIMQELPAVEDLLETSDDAPIIRMLNALLTQAARDGASDIHIEPYERHSSVRFRVDGTLREVVQPNRALH
AALISRLKIMADLDISEKRLPQDGRISLRIGTRAVDVRVSTLPSAHGERAVLRLLDKSESKLSLESVGMQGDVLHRFEHL
IAQPHGIILVTGPTGSGKTTTLYAALQRLDASASNIMTVEDPIEYELAGIGQTQVNSKIDLSFAKALRAILRQDPDVIMI
GEIRDFETAQIAIQASLTGHLVLATLHTNDASSAVTRLTDIGVEPFLLSSSLLGVLAQRLVRKRCTYCHASSAGPGLAAG
CEHCGYTGYAGRTGVFELLVTDDAVRAQIHNRASEAEIRAAALQAGMMLMRDDGERLVTAGVTSREELLRVTPD

Sequences:

>Translated_474_residues
MRYPLPYAFARSNTLLLEDDGHALTLWHPARPDPAALGEVMRKFAAHSPALVLQCADAGTLTQRISAAYAAGESSAAAVV
SEVESDADLSRIMQELPAVEDLLETSDDAPIIRMLNALLTQAARDGASDIHIEPYERHSSVRFRVDGTLREVVQPNRALH
AALISRLKIMADLDISEKRLPQDGRISLRIGTRAVDVRVSTLPSAHGERAVLRLLDKSESKLSLESVGMQGDVLHRFEHL
IAQPHGIILVTGPTGSGKTTTLYAALQRLDASASNIMTVEDPIEYELAGIGQTQVNSKIDLSFAKALRAILRQDPDVIMI
GEIRDFETAQIAIQASLTGHLVLATLHTNDASSAVTRLTDIGVEPFLLSSSLLGVLAQRLVRKRCTYCHASSAGPGLAAG
CEHCGYTGYAGRTGVFELLVTDDAVRAQIHNRASEAEIRAAALQAGMMLMRDDGERLVTAGVTSREELLRVTPD
>Mature_474_residues
MRYPLPYAFARSNTLLLEDDGHALTLWHPARPDPAALGEVMRKFAAHSPALVLQCADAGTLTQRISAAYAAGESSAAAVV
SEVESDADLSRIMQELPAVEDLLETSDDAPIIRMLNALLTQAARDGASDIHIEPYERHSSVRFRVDGTLREVVQPNRALH
AALISRLKIMADLDISEKRLPQDGRISLRIGTRAVDVRVSTLPSAHGERAVLRLLDKSESKLSLESVGMQGDVLHRFEHL
IAQPHGIILVTGPTGSGKTTTLYAALQRLDASASNIMTVEDPIEYELAGIGQTQVNSKIDLSFAKALRAILRQDPDVIMI
GEIRDFETAQIAIQASLTGHLVLATLHTNDASSAVTRLTDIGVEPFLLSSSLLGVLAQRLVRKRCTYCHASSAGPGLAAG
CEHCGYTGYAGRTGVFELLVTDDAVRAQIHNRASEAEIRAAALQAGMMLMRDDGERLVTAGVTSREELLRVTPD

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of pullulanase [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=440, Percent_Identity=53.4090909090909, Blast_Score=461, Evalue=1e-131,
Organism=Escherichia coli, GI1786296, Length=399, Percent_Identity=45.1127819548872, Blast_Score=320, Evalue=2e-88,
Organism=Escherichia coli, GI87082188, Length=241, Percent_Identity=39.0041493775934, Blast_Score=115, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 51137; Mature: 51137

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYPLPYAFARSNTLLLEDDGHALTLWHPARPDPAALGEVMRKFAAHSPALVLQCADAGT
CCCCCCCCCCCCCEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCH
LTQRISAAYAAGESSAAAVVSEVESDADLSRIMQELPAVEDLLETSDDAPIIRMLNALLT
HHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHH
QAARDGASDIHIEPYERHSSVRFRVDGTLREVVQPNRALHAALISRLKIMADLDISEKRL
HHHCCCCCCCCCCCCCCCCCEEEEECCHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHC
PQDGRISLRIGTRAVDVRVSTLPSAHGERAVLRLLDKSESKLSLESVGMQGDVLHRFEHL
CCCCEEEEEECCEEEEEEEECCCCCCCHHHHHHHHHCCCCCEEHHHCCCCHHHHHHHHHH
IAQPHGIILVTGPTGSGKTTTLYAALQRLDASASNIMTVEDPIEYELAGIGQTQVNSKID
HCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCEEEECCCCHHCCCEEH
LSFAKALRAILRQDPDVIMIGEIRDFETAQIAIQASLTGHLVLATLHTNDASSAVTRLTD
HHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEEEECCCEEEEEEEECCCHHHHHHHHHH
IGVEPFLLSSSLLGVLAQRLVRKRCTYCHASSAGPGLAAGCEHCGYTGYAGRTGVFELLV
CCCCHHHHHHHHHHHHHHHHHHHHHCEEECCCCCCCHHHHHHHCCCCCCCCCCCEEEEEE
TDDAVRAQIHNRASEAEIRAAALQAGMMLMRDDGERLVTAGVTSREELLRVTPD
ECHHHHHHHHHCCHHHHHHHHHHHHCEEEEECCCCEEEEECCCCHHHHEECCCC
>Mature Secondary Structure
MRYPLPYAFARSNTLLLEDDGHALTLWHPARPDPAALGEVMRKFAAHSPALVLQCADAGT
CCCCCCCCCCCCCEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCH
LTQRISAAYAAGESSAAAVVSEVESDADLSRIMQELPAVEDLLETSDDAPIIRMLNALLT
HHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHH
QAARDGASDIHIEPYERHSSVRFRVDGTLREVVQPNRALHAALISRLKIMADLDISEKRL
HHHCCCCCCCCCCCCCCCCCEEEEECCHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHC
PQDGRISLRIGTRAVDVRVSTLPSAHGERAVLRLLDKSESKLSLESVGMQGDVLHRFEHL
CCCCEEEEEECCEEEEEEEECCCCCCCHHHHHHHHHCCCCCEEHHHCCCCHHHHHHHHHH
IAQPHGIILVTGPTGSGKTTTLYAALQRLDASASNIMTVEDPIEYELAGIGQTQVNSKID
HCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCEEEECCCCHHCCCEEH
LSFAKALRAILRQDPDVIMIGEIRDFETAQIAIQASLTGHLVLATLHTNDASSAVTRLTD
HHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEEEECCCEEEEEEEECCCHHHHHHHHHH
IGVEPFLLSSSLLGVLAQRLVRKRCTYCHASSAGPGLAAGCEHCGYTGYAGRTGVFELLV
CCCCHHHHHHHHHHHHHHHHHHHHHCEEECCCCCCCHHHHHHHCCCCCCCCCCCEEEEEE
TDDAVRAQIHNRASEAEIRAAALQAGMMLMRDDGERLVTAGVTSREELLRVTPD
ECHHHHHHHHHCCHHHHHHHHHHHHCEEEEECCCCEEEEECCCCHHHHEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1738317; 2677007 [H]