Click here to switch to the map view.

The map label for this gene is braD [H]

Identifier: 91786887

GI number: 91786887

Start: 1004524

End: 1005441

Strand: Direct

Name: braD [H]

Synonym: Bpro_0987

Alternate gene names: 91786887

Gene position: 1004524-1005441 (Clockwise)

Preceding gene: 91786886

Following gene: 91786888

Centisome position: 19.32

GC content: 66.56

Gene sequence:

>918_bases
ATGAACAGCACGCTTGTTTTCGCGCAGCTGCTCAACGGCCTGCAATACGGCGTCCTGCTGTTTCTGCTGGCTGCAGGCCT
GACGCTGGTCTTCGGCATCATGAGCTTCGTCAACCTGGCGCACGGCTCGCTCTACATGATGGGCGCGTACTTCGCCGCCA
CGGCCTTCCAGTACACCGGCTCCTTCGGGCTGGCCGTGCTGGCGGCCATGCTGGGCTCGCTGCTGCTGGGCCTGGCGCTG
GAGTTCGTCGCCGTGGCGCGCCTGTACCGCCGCGACCACCTCGACCATGTGCTGGCCACCTTCGGCCTGGTGCTGTTCTT
CAATGAACTGGTGCGCATCATCTGGGGAGCGCAGCCGGTATTTCTGCAGGTTCCGGAATTCCTCGGCGGCGCCATCGACA
TCGGCGGCTTCAGCTACCCGGCCTACCGGTTCGCCATCATCGTCGTCGGTCTGGTCGTGGCGATCGGTTCCTGGCTGCTG
ATCAACAAGACCCGCGTGGGCATGCTGATCCGCGCCGGCGCCGTCAACCCCGCGATGGTGGCGGCCCTGGGCGTCAACAT
TCGCCTGCTCAACGCCATGCTGTTTGCGGTCGGCGCCATGCTCGCGGGTCTGGCGGGCGCGATGGCCGGACCCATCCTGT
CGGTGCAGTCCGGCATGGGCGAGTCGGTGCTGATCACCACGCTGGTCGTCATCGTGATCGGCGGCATCGGCTCGGTCACC
GGCGCGTTTTACGCGGCGCTCATCGTCGGCATCGTCGACACCATGGGCCGCGTATTTCTGCCCTTCCTGCTGCGCCAGGT
CACCGAGCGGTCGTTCGCCGACGCGGCCGGCCCGGCGCTGGCCTCCATGCTGGTGTACCTGCTGATGGCGGTGGTGCTGG
CGTGGCGCCCGCAAGGTCTTTTTCCGGCGAACAGGTGA

Upstream 100 bases:

>100_bases
ACCATCAAGAGCGGCAGCAGCGTCGTCGGAAAGCTCGTCGCAACGCCGCTGACCCGGCAGACCGATGCCTACCACGCACA
ATGCGCGCTGAAGTGACACC

Downstream 100 bases:

>100_bases
AGGTGATGGCGATGACTCCCCTTATTTCACGGCCCAGCGCCTGGGTTCCCCTGCTGGCGCTGCTGCTGCTGGCGACCGTT
CCCTTTGTGGCGCAGGCCAC

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MNSTLVFAQLLNGLQYGVLLFLLAAGLTLVFGIMSFVNLAHGSLYMMGAYFAATAFQYTGSFGLAVLAAMLGSLLLGLAL
EFVAVARLYRRDHLDHVLATFGLVLFFNELVRIIWGAQPVFLQVPEFLGGAIDIGGFSYPAYRFAIIVVGLVVAIGSWLL
INKTRVGMLIRAGAVNPAMVAALGVNIRLLNAMLFAVGAMLAGLAGAMAGPILSVQSGMGESVLITTLVVIVIGGIGSVT
GAFYAALIVGIVDTMGRVFLPFLLRQVTERSFADAAGPALASMLVYLLMAVVLAWRPQGLFPANR

Sequences:

>Translated_305_residues
MNSTLVFAQLLNGLQYGVLLFLLAAGLTLVFGIMSFVNLAHGSLYMMGAYFAATAFQYTGSFGLAVLAAMLGSLLLGLAL
EFVAVARLYRRDHLDHVLATFGLVLFFNELVRIIWGAQPVFLQVPEFLGGAIDIGGFSYPAYRFAIIVVGLVVAIGSWLL
INKTRVGMLIRAGAVNPAMVAALGVNIRLLNAMLFAVGAMLAGLAGAMAGPILSVQSGMGESVLITTLVVIVIGGIGSVT
GAFYAALIVGIVDTMGRVFLPFLLRQVTERSFADAAGPALASMLVYLLMAVVLAWRPQGLFPANR
>Mature_305_residues
MNSTLVFAQLLNGLQYGVLLFLLAAGLTLVFGIMSFVNLAHGSLYMMGAYFAATAFQYTGSFGLAVLAAMLGSLLLGLAL
EFVAVARLYRRDHLDHVLATFGLVLFFNELVRIIWGAQPVFLQVPEFLGGAIDIGGFSYPAYRFAIIVVGLVVAIGSWLL
INKTRVGMLIRAGAVNPAMVAALGVNIRLLNAMLFAVGAMLAGLAGAMAGPILSVQSGMGESVLITTLVVIVIGGIGSVT
GAFYAALIVGIVDTMGRVFLPFLLRQVTERSFADAAGPALASMLVYLLMAVVLAWRPQGLFPANR

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0559

COG function: function code E; Branched-chain amino acid ABC-type transport system, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789866, Length=311, Percent_Identity=27.9742765273312, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851 [H]

Pfam domain/function: PF02653 BPD_transp_2 [H]

EC number: NA

Molecular weight: Translated: 32166; Mature: 32166

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSTLVFAQLLNGLQYGVLLFLLAAGLTLVFGIMSFVNLAHGSLYMMGAYFAATAFQYTG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCC
SFGLAVLAAMLGSLLLGLALEFVAVARLYRRDHLDHVLATFGLVLFFNELVRIIWGAQPV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
FLQVPEFLGGAIDIGGFSYPAYRFAIIVVGLVVAIGSWLLINKTRVGMLIRAGAVNPAMV
HEECHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHCCHHHEEHHHHHHHHCCCCCCHHHH
AALGVNIRLLNAMLFAVGAMLAGLAGAMAGPILSVQSGMGESVLITTLVVIVIGGIGSVT
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHCCCHHHH
GAFYAALIVGIVDTMGRVFLPFLLRQVTERSFADAAGPALASMLVYLLMAVVLAWRPQGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCC
FPANR
CCCCC
>Mature Secondary Structure
MNSTLVFAQLLNGLQYGVLLFLLAAGLTLVFGIMSFVNLAHGSLYMMGAYFAATAFQYTG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCC
SFGLAVLAAMLGSLLLGLALEFVAVARLYRRDHLDHVLATFGLVLFFNELVRIIWGAQPV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
FLQVPEFLGGAIDIGGFSYPAYRFAIIVVGLVVAIGSWLLINKTRVGMLIRAGAVNPAMV
HEECHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHCCHHHEEHHHHHHHHCCCCCCHHHH
AALGVNIRLLNAMLFAVGAMLAGLAGAMAGPILSVQSGMGESVLITTLVVIVIGGIGSVT
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHCCCHHHH
GAFYAALIVGIVDTMGRVFLPFLLRQVTERSFADAAGPALASMLVYLLMAVVLAWRPQGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCC
FPANR
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043; 2509433 [H]