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The map label for this gene is bphA1 [H]

Identifier: 91786883

GI number: 91786883

Start: 1001130

End: 1002407

Strand: Direct

Name: bphA1 [H]

Synonym: Bpro_0983

Alternate gene names: 91786883

Gene position: 1001130-1002407 (Clockwise)

Preceding gene: 91786882

Following gene: 91786884

Centisome position: 19.25

GC content: 61.66

Gene sequence:

>1278_bases
ATGACTGCATCGACCACCGCATCCACCGGAACCTTTCCCATCAAGATCCATTGGGAATCCGAAGGCACCAGCCGGATTCC
CTTCATGGCGTACACCGACCCGGACCAGCACAAGAAAGAGCTCGAACGCTTCTTCTACAACAAACACTGGAACTACGTCG
GCCTCGATGCCGAGATCCCCAACGCCGGCGACTTCAAGCGCACGGTGGTCGGAGAGCGCTCGGTGATCATGGTGCGCGAC
GCCGACGGCGGCGTCAGTGTGGTCGAGAACGTCTGTGCCCACCGCGGCATGCAGTTTTGCCGCGAACGCCATGGCAACAA
AAAAGAGTTTGTCTGCCCGTACCACCAGTGGAGCTATACGCTCAAGGGCGATTTGCAGGGTGTGCCGTTTCGCCGCGGCG
TCAAGCAGGACGGCAAGGTCAACGGCGGCATGCCGGCGGACTTCAAGACCAGCGACCATGGCCTCAACAAGCTCAAGGTC
GCGACGCGCGGCGGCGTGGTGTTTGCCAGCTTTGACCACGAGATTGAATCGCTCGAAGACTTCCTGGGACCGGTCATCCT
CGGTTATTTCGATCGCCTATTCGACGGCCGCGAGCTGAAAATCCTGGGCTACAACCGCCAGCGCATTCCGGGCAACTGGA
AGCTGATGCAGGAGAACATCAAGGACCCGTACCACCCCGGCCTGCTGCACACCTGGTTTGTCACCTTCGGCCTCTGGCGC
GCTGACAACAAGTCAGAACTGAAAATGGATGCACGCCACCGCCATGCGGCCATGATTTCCACGCGCGGACAGGCGGGCAA
GGCCGATCAGGTCACCCAGGTGTCCAGCTTCAAGGAGAGCATGGAGCTCAACGACCCGCGCTTCCTCGACATCGTGCCCG
AGCCATGGTGGGGCGGCCCGACCGCGGTGATGATGACGCTGTTCCCCAGCGTCATCCTGCAGCAGCAGGTCAACAGCGTA
TCGACGCGCCACATCCAGCCCGACGGCCACGGCTCCTTCGATTTCGTGTGGACTCATTTCGGCTTCGCGGACGACACGCA
AGAGATGACGCAGCGCCGGCTGCGCCAGGCCAACCTGTTCGGTCCGGCCGGCTTCGTGTCGGCCGACGACGGCGAGGTGA
TCGAGTTCTCGCAGCAAGGCTTCGAGCAGAAGCCTTACCACCGCACCCTGGCCGAACTGGGCGGGCGCACTGTCGAGGAC
ACCGACCACATGGTCACCGAAACACTGATACGCGGCATGTACCAATACTGGCGCGAAGTGATGGAGGCTGAGGCATGA

Upstream 100 bases:

>100_bases
ATCGCGGGCCTGGCCGGCATCACGGTCACGGTGAAGGCCTGACACGCGGGCGCGGAAAACGGAAAACGGACAACGGACAA
CGGAAACCAAGGAGACAGCG

Downstream 100 bases:

>100_bases
GCGCCGCCCGGCCGCCCGAAGGCGTTCGCACCGCAGTGCACAGCACGGAGGTTATCTAATGAGCACCGCTGTCGACATCA
ATTTTGAAGACTACCTGCAG

Product: Rieske (2Fe-2S) domain-containing protein

Products: NA

Alternate protein names: Biphenyl 2,3-dioxygenase [H]

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MTASTTASTGTFPIKIHWESEGTSRIPFMAYTDPDQHKKELERFFYNKHWNYVGLDAEIPNAGDFKRTVVGERSVIMVRD
ADGGVSVVENVCAHRGMQFCRERHGNKKEFVCPYHQWSYTLKGDLQGVPFRRGVKQDGKVNGGMPADFKTSDHGLNKLKV
ATRGGVVFASFDHEIESLEDFLGPVILGYFDRLFDGRELKILGYNRQRIPGNWKLMQENIKDPYHPGLLHTWFVTFGLWR
ADNKSELKMDARHRHAAMISTRGQAGKADQVTQVSSFKESMELNDPRFLDIVPEPWWGGPTAVMMTLFPSVILQQQVNSV
STRHIQPDGHGSFDFVWTHFGFADDTQEMTQRRLRQANLFGPAGFVSADDGEVIEFSQQGFEQKPYHRTLAELGGRTVED
TDHMVTETLIRGMYQYWREVMEAEA

Sequences:

>Translated_425_residues
MTASTTASTGTFPIKIHWESEGTSRIPFMAYTDPDQHKKELERFFYNKHWNYVGLDAEIPNAGDFKRTVVGERSVIMVRD
ADGGVSVVENVCAHRGMQFCRERHGNKKEFVCPYHQWSYTLKGDLQGVPFRRGVKQDGKVNGGMPADFKTSDHGLNKLKV
ATRGGVVFASFDHEIESLEDFLGPVILGYFDRLFDGRELKILGYNRQRIPGNWKLMQENIKDPYHPGLLHTWFVTFGLWR
ADNKSELKMDARHRHAAMISTRGQAGKADQVTQVSSFKESMELNDPRFLDIVPEPWWGGPTAVMMTLFPSVILQQQVNSV
STRHIQPDGHGSFDFVWTHFGFADDTQEMTQRRLRQANLFGPAGFVSADDGEVIEFSQQGFEQKPYHRTLAELGGRTVED
TDHMVTETLIRGMYQYWREVMEAEA
>Mature_424_residues
TASTTASTGTFPIKIHWESEGTSRIPFMAYTDPDQHKKELERFFYNKHWNYVGLDAEIPNAGDFKRTVVGERSVIMVRDA
DGGVSVVENVCAHRGMQFCRERHGNKKEFVCPYHQWSYTLKGDLQGVPFRRGVKQDGKVNGGMPADFKTSDHGLNKLKVA
TRGGVVFASFDHEIESLEDFLGPVILGYFDRLFDGRELKILGYNRQRIPGNWKLMQENIKDPYHPGLLHTWFVTFGLWRA
DNKSELKMDARHRHAAMISTRGQAGKADQVTQVSSFKESMELNDPRFLDIVPEPWWGGPTAVMMTLFPSVILQQQVNSVS
TRHIQPDGHGSFDFVWTHFGFADDTQEMTQRRLRQANLFGPAGFVSADDGEVIEFSQQGFEQKPYHRTLAELGGRTVEDT
DHMVTETLIRGMYQYWREVMEAEA

Specific function: Part Of The Multicomponent 3-Phenylpropionate Dioxygenase. Converts 3-Phenylpropionic Acid (Pp) Into Cis-3-(3- Carboxyethyl)-3,5-Cyclohexadiene-1,2-Diol (Pp-Dihydrodiol). [C]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Escherichia coli, GI1788888, Length=432, Percent_Identity=27.5462962962963, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI1788103, Length=209, Percent_Identity=30.1435406698565, Blast_Score=92, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017941
- InterPro:   IPR015881
- InterPro:   IPR015879
- InterPro:   IPR001663 [H]

Pfam domain/function: PF00355 Rieske; PF00848 Ring_hydroxyl_A [H]

EC number: =1.14.12.18 [H]

Molecular weight: Translated: 48508; Mature: 48377

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS00570 RING_HYDROXYL_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASTTASTGTFPIKIHWESEGTSRIPFMAYTDPDQHKKELERFFYNKHWNYVGLDAEIP
CCCCCCCCCCEEEEEEEECCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCEEECCCCCC
NAGDFKRTVVGERSVIMVRDADGGVSVVENVCAHRGMQFCRERHGNKKEFVCPYHQWSYT
CCCCCHHHEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECHHHCEEE
LKGDLQGVPFRRGVKQDGKVNGGMPADFKTSDHGLNKLKVATRGGVVFASFDHEIESLED
EECCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEECCHHHHHHHH
FLGPVILGYFDRLFDGRELKILGYNRQRIPGNWKLMQENIKDPYHPGLLHTWFVTFGLWR
HHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHC
ADNKSELKMDARHRHAAMISTRGQAGKADQVTQVSSFKESMELNDPRFLDIVPEPWWGGP
CCCHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCH
TAVMMTLFPSVILQQQVNSVSTRHIQPDGHGSFDFVWTHFGFADDTQEMTQRRLRQANLF
HHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCC
GPAGFVSADDGEVIEFSQQGFEQKPYHRTLAELGGRTVEDTDHMVTETLIRGMYQYWREV
CCCCCCCCCCCCEEEEHHCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
MEAEA
HHCCC
>Mature Secondary Structure 
TASTTASTGTFPIKIHWESEGTSRIPFMAYTDPDQHKKELERFFYNKHWNYVGLDAEIP
CCCCCCCCCEEEEEEEECCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCEEECCCCCC
NAGDFKRTVVGERSVIMVRDADGGVSVVENVCAHRGMQFCRERHGNKKEFVCPYHQWSYT
CCCCCHHHEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECHHHCEEE
LKGDLQGVPFRRGVKQDGKVNGGMPADFKTSDHGLNKLKVATRGGVVFASFDHEIESLED
EECCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEECCHHHHHHHH
FLGPVILGYFDRLFDGRELKILGYNRQRIPGNWKLMQENIKDPYHPGLLHTWFVTFGLWR
HHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHC
ADNKSELKMDARHRHAAMISTRGQAGKADQVTQVSSFKESMELNDPRFLDIVPEPWWGGP
CCCHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCH
TAVMMTLFPSVILQQQVNSVSTRHIQPDGHGSFDFVWTHFGFADDTQEMTQRRLRQANLF
HHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCC
GPAGFVSADDGEVIEFSQQGFEQKPYHRTLAELGGRTVEDTDHMVTETLIRGMYQYWREV
CCCCCCCCCCCCEEEEHHCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
MEAEA
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8048958 [H]