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The map label for this gene is hyfB [H]

Identifier: 91786818

GI number: 91786818

Start: 932026

End: 934050

Strand: Direct

Name: hyfB [H]

Synonym: Bpro_0916

Alternate gene names: 91786818

Gene position: 932026-934050 (Clockwise)

Preceding gene: 91786817

Following gene: 91786819

Centisome position: 17.92

GC content: 65.53

Gene sequence:

>2025_bases
ATGAGCAGCCTGCCCCCCATCGCGCAATGGTTCATCCTCGACTGGATGCTCGCGGTTGTGCTGGGCTGGCTGATCGTGGG
GGTCGCAGGGGTCATGGCGCTGCATCGACTCGCCCTGGTGTCCCGCGTGCTTTTCCCGGTCGGCGGCTTGCTCGGGCTGG
CGCTGTTCGGCCTGGCCTTCGGCGCGGTGCCCGGCACGCCTGAAGTCGCCGTGCTCCCCATCGGCCTGCCGGACTTGCCG
TTCCACCTGCGCCTGGATAGCCTCTCGGCCTACTTCCTGATGGTGATAGGCGCGGCGTCGGCCGGTATCTCCGCCTTCGC
GGCCGGCTACTTCCGCAAGGGCGAAGGCACGTCGCCGGGCCTGCTGTGCCTGGAGTACCACGTCTTCCTGGCCAGCATGG
TGGGGGTGGTCCTGGCCGACGATGCCTATTCGTTCATGGTGATGTGGGAGACGATGGCGCTGTCGTCGTTCTTCCTGGTG
ACCGCGAACCACCGCATCCCGGAAGTGCGTAGCGCCGGCTACCTCTACATCACGATGGCGCGCATCGGTGCGATCGCCAT
CTTGCTGTGCTTCGGCGTGCTGCAGGCCAACACCGGCGACTACACCTTCGCCAACATGCGGGCGCAGGCCCCGACACCTT
TCTGGGCCTCGCTCGGCTTCCTGCTCGCCGTGTTTGGTTTCGGCGCCAAGGCCGGCATCCTGCCCTTGCATGTATGGCTG
CCAGAGGCCCACCCGGCCGCACCCTCGCCGGTGTCGGCCCTGATGAGCGGTGTCATGCTCAATACGGCCATCTACGGTCT
GATGCGCGTGTCTTTCGACTTGCTGCACATGCGTATGTGGTGGTGGGGCGGGCTGCTGCTGGCCGTTGGCCTGGCTACCG
CCCTGTTCGGCGTGGTCTTCGCGGCGGTGCAGACCGACATGAAGCGGTTGCTGGCCTATTCGTCGATCGAGAACATGGGC
CTGCTGTTCGTCGGCATTGGCCTGACGCTGATCTTTTCGGCCTACGACATGAATCCGATGGCAGCACTGGCCATGACGGC
GACGCTCTATCACGTCGCAAGCCATGCCTGCTTCAAGAGTCTGCTCTTCCTCGGCACCGGCAGCGTCCTGCACGCCACGT
CCGAGAGAAACCTCGGCAAGCTGGGAGGGTTGATCCGCACCATGCCCTGGGTCGGCTGGCTGACACTGATCGGCGTGCTG
GCCAGCGCCGGTCTGCCGCCACTCGGCGGGTTCGTGTCGGAGTGGTTGTTGCTGCAGAGCTTTTTATTCACGCCGAGCCT
GCCGTTGCCGCTGTTGACCATGCTGATCCCTGTGGTCGCCGCGCTGATCGCCCTGGTGGCAGCACTGGCTGGCTACACGA
TGGTCAAGTTCTTTGGAGTGATTTTCCTCGGCCAGCCGCGCGAGGGCAAGCTCGCCGGTGCCCACGACGCTGGACGCTGG
GAGATAACGGGCATGGTGTGGCTGGCGCTCGGCTGCGTCGCGCTCGGCCTGCTGCCGACACAGTTCATTCAACTGATAGA
CCCCGTGACGCAGCAGCTCGTCGCGTCGGGTCTGGGTGCCAAGGTGGCGGCTGGCGGCTGGCTGCTGGCGCCGAACAGTC
TCGAGCAGGCGAGCTACGGGCCGGTGATTTTCCTGCTCGGCATCCTGGGCAGCTTCGCGCTGGCTTACCTGCTGGTGCGC
TGGCTCTACCACGGCCGGGTGCGTCGCGGCCTGCCTTGGGCCTGTGGCTTCCCGTGGGGCAACGCCCGCATGCAGGATAC
GGCAGAGGGCTTCGGCCAGCCGATCCGCCAGATCTTCGAGCCCTTCTTCCTGATGCAGCGCGAGCTGCCGTCGCCCTTTG
ACGAACAGCCGCGGTATCGCGTCAGGGTCGAGGATCACTTCTGGCGCTGGCTCTATGTACCCATTGCCGACCTGGCCGCG
TACCTGGCGCGACTGATCGGCCTGATGCAGCAGGGGCGCATCGCGGTGTACCTGCTGTACAGTTTTCTAACCCTGATCGC
CACGCTGCTGGCGGTGATGCGATGA

Upstream 100 bases:

>100_bases
CCGGGCCGGTTGGTCATGTTGCGCCAGCCTTGGCGAAACCAGCCTCAATACGCGATAAACCACCTCCTGTGAAGCGACGT
GTAGCGCGCCGGCGTGGGGC

Downstream 100 bases:

>100_bases
GGCGGTCATGAGCACGGCGCCGGACCGGTTCCATGCAGTGCTGCGCCCCCCGGGGGCCGGGCGCGCGGCGGCCTGGGGGC
AGACATGACCTTCTCCGGCT

Product: hydrogenase 4 subunit B

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 674; Mature: 673

Protein sequence:

>674_residues
MSSLPPIAQWFILDWMLAVVLGWLIVGVAGVMALHRLALVSRVLFPVGGLLGLALFGLAFGAVPGTPEVAVLPIGLPDLP
FHLRLDSLSAYFLMVIGAASAGISAFAAGYFRKGEGTSPGLLCLEYHVFLASMVGVVLADDAYSFMVMWETMALSSFFLV
TANHRIPEVRSAGYLYITMARIGAIAILLCFGVLQANTGDYTFANMRAQAPTPFWASLGFLLAVFGFGAKAGILPLHVWL
PEAHPAAPSPVSALMSGVMLNTAIYGLMRVSFDLLHMRMWWWGGLLLAVGLATALFGVVFAAVQTDMKRLLAYSSIENMG
LLFVGIGLTLIFSAYDMNPMAALAMTATLYHVASHACFKSLLFLGTGSVLHATSERNLGKLGGLIRTMPWVGWLTLIGVL
ASAGLPPLGGFVSEWLLLQSFLFTPSLPLPLLTMLIPVVAALIALVAALAGYTMVKFFGVIFLGQPREGKLAGAHDAGRW
EITGMVWLALGCVALGLLPTQFIQLIDPVTQQLVASGLGAKVAAGGWLLAPNSLEQASYGPVIFLLGILGSFALAYLLVR
WLYHGRVRRGLPWACGFPWGNARMQDTAEGFGQPIRQIFEPFFLMQRELPSPFDEQPRYRVRVEDHFWRWLYVPIADLAA
YLARLIGLMQQGRIAVYLLYSFLTLIATLLAVMR

Sequences:

>Translated_674_residues
MSSLPPIAQWFILDWMLAVVLGWLIVGVAGVMALHRLALVSRVLFPVGGLLGLALFGLAFGAVPGTPEVAVLPIGLPDLP
FHLRLDSLSAYFLMVIGAASAGISAFAAGYFRKGEGTSPGLLCLEYHVFLASMVGVVLADDAYSFMVMWETMALSSFFLV
TANHRIPEVRSAGYLYITMARIGAIAILLCFGVLQANTGDYTFANMRAQAPTPFWASLGFLLAVFGFGAKAGILPLHVWL
PEAHPAAPSPVSALMSGVMLNTAIYGLMRVSFDLLHMRMWWWGGLLLAVGLATALFGVVFAAVQTDMKRLLAYSSIENMG
LLFVGIGLTLIFSAYDMNPMAALAMTATLYHVASHACFKSLLFLGTGSVLHATSERNLGKLGGLIRTMPWVGWLTLIGVL
ASAGLPPLGGFVSEWLLLQSFLFTPSLPLPLLTMLIPVVAALIALVAALAGYTMVKFFGVIFLGQPREGKLAGAHDAGRW
EITGMVWLALGCVALGLLPTQFIQLIDPVTQQLVASGLGAKVAAGGWLLAPNSLEQASYGPVIFLLGILGSFALAYLLVR
WLYHGRVRRGLPWACGFPWGNARMQDTAEGFGQPIRQIFEPFFLMQRELPSPFDEQPRYRVRVEDHFWRWLYVPIADLAA
YLARLIGLMQQGRIAVYLLYSFLTLIATLLAVMR
>Mature_673_residues
SSLPPIAQWFILDWMLAVVLGWLIVGVAGVMALHRLALVSRVLFPVGGLLGLALFGLAFGAVPGTPEVAVLPIGLPDLPF
HLRLDSLSAYFLMVIGAASAGISAFAAGYFRKGEGTSPGLLCLEYHVFLASMVGVVLADDAYSFMVMWETMALSSFFLVT
ANHRIPEVRSAGYLYITMARIGAIAILLCFGVLQANTGDYTFANMRAQAPTPFWASLGFLLAVFGFGAKAGILPLHVWLP
EAHPAAPSPVSALMSGVMLNTAIYGLMRVSFDLLHMRMWWWGGLLLAVGLATALFGVVFAAVQTDMKRLLAYSSIENMGL
LFVGIGLTLIFSAYDMNPMAALAMTATLYHVASHACFKSLLFLGTGSVLHATSERNLGKLGGLIRTMPWVGWLTLIGVLA
SAGLPPLGGFVSEWLLLQSFLFTPSLPLPLLTMLIPVVAALIALVAALAGYTMVKFFGVIFLGQPREGKLAGAHDAGRWE
ITGMVWLALGCVALGLLPTQFIQLIDPVTQQLVASGLGAKVAAGGWLLAPNSLEQASYGPVIFLLGILGSFALAYLLVRW
LYHGRVRRGLPWACGFPWGNARMQDTAEGFGQPIRQIFEPFFLMQRELPSPFDEQPRYRVRVEDHFWRWLYVPIADLAAY
LARLIGLMQQGRIAVYLLYSFLTLIATLLAVMR

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=437, Percent_Identity=27.6887871853547, Blast_Score=116, Evalue=6e-26,
Organism=Escherichia coli, GI1788827, Length=611, Percent_Identity=34.3698854337152, Blast_Score=320, Evalue=3e-88,
Organism=Escherichia coli, GI2367154, Length=557, Percent_Identity=30.7001795332136, Blast_Score=206, Evalue=5e-54,
Organism=Escherichia coli, GI1788831, Length=364, Percent_Identity=34.0659340659341, Blast_Score=154, Evalue=2e-38,
Organism=Escherichia coli, GI1788614, Length=360, Percent_Identity=32.7777777777778, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1788613, Length=348, Percent_Identity=29.5977011494253, Blast_Score=121, Evalue=2e-28,
Organism=Escherichia coli, GI1788829, Length=398, Percent_Identity=28.1407035175879, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI145693160, Length=265, Percent_Identity=26.0377358490566, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 72992; Mature: 72861

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLPPIAQWFILDWMLAVVLGWLIVGVAGVMALHRLALVSRVLFPVGGLLGLALFGLAF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAVPGTPEVAVLPIGLPDLPFHLRLDSLSAYFLMVIGAASAGISAFAAGYFRKGEGTSPG
CCCCCCCCEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
LLCLEYHVFLASMVGVVLADDAYSFMVMWETMALSSFFLVTANHRIPEVRSAGYLYITMA
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEECCCCCCCHHCCCEEEEHHH
RIGAIAILLCFGVLQANTGDYTFANMRAQAPTPFWASLGFLLAVFGFGAKAGILPLHVWL
HHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEC
PEAHPAAPSPVSALMSGVMLNTAIYGLMRVSFDLLHMRMWWWGGLLLAVGLATALFGVVF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAVQTDMKRLLAYSSIENMGLLFVGIGLTLIFSAYDMNPMAALAMTATLYHVASHACFKS
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
LLFLGTGSVLHATSERNLGKLGGLIRTMPWVGWLTLIGVLASAGLPPLGGFVSEWLLLQS
HHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
FLFTPSLPLPLLTMLIPVVAALIALVAALAGYTMVKFFGVIFLGQPREGKLAGAHDAGRW
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCE
EITGMVWLALGCVALGLLPTQFIQLIDPVTQQLVASGLGAKVAAGGWLLAPNSLEQASYG
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCEEECCCCCCCCCCC
PVIFLLGILGSFALAYLLVRWLYHGRVRRGLPWACGFPWGNARMQDTAEGFGQPIRQIFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
PFFLMQRELPSPFDEQPRYRVRVEDHFWRWLYVPIADLAAYLARLIGLMQQGRIAVYLLY
HHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
SFLTLIATLLAVMR
HHHHHHHHHHHHHC
>Mature Secondary Structure 
SSLPPIAQWFILDWMLAVVLGWLIVGVAGVMALHRLALVSRVLFPVGGLLGLALFGLAF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAVPGTPEVAVLPIGLPDLPFHLRLDSLSAYFLMVIGAASAGISAFAAGYFRKGEGTSPG
CCCCCCCCEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
LLCLEYHVFLASMVGVVLADDAYSFMVMWETMALSSFFLVTANHRIPEVRSAGYLYITMA
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEECCCCCCCHHCCCEEEEHHH
RIGAIAILLCFGVLQANTGDYTFANMRAQAPTPFWASLGFLLAVFGFGAKAGILPLHVWL
HHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEC
PEAHPAAPSPVSALMSGVMLNTAIYGLMRVSFDLLHMRMWWWGGLLLAVGLATALFGVVF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAVQTDMKRLLAYSSIENMGLLFVGIGLTLIFSAYDMNPMAALAMTATLYHVASHACFKS
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
LLFLGTGSVLHATSERNLGKLGGLIRTMPWVGWLTLIGVLASAGLPPLGGFVSEWLLLQS
HHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
FLFTPSLPLPLLTMLIPVVAALIALVAALAGYTMVKFFGVIFLGQPREGKLAGAHDAGRW
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCE
EITGMVWLALGCVALGLLPTQFIQLIDPVTQQLVASGLGAKVAAGGWLLAPNSLEQASYG
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCEEECCCCCCCCCCC
PVIFLLGILGSFALAYLLVRWLYHGRVRRGLPWACGFPWGNARMQDTAEGFGQPIRQIFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
PFFLMQRELPSPFDEQPRYRVRVEDHFWRWLYVPIADLAAYLARLIGLMQQGRIAVYLLY
HHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
SFLTLIATLLAVMR
HHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 2016588 [H]