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The map label for this gene is hisF [H]

Identifier: 91786711

GI number: 91786711

Start: 834848

End: 835627

Strand: Direct

Name: hisF [H]

Synonym: Bpro_0809

Alternate gene names: 91786711

Gene position: 834848-835627 (Clockwise)

Preceding gene: 91786710

Following gene: 91786712

Centisome position: 16.05

GC content: 65.51

Gene sequence:

>780_bases
ATGTTAGCCAAACGAATCATCCCTTGCCTGGACGTCACCGGCGGCCGTGTGGTCAAGGGCGTCAACTTTGTCGAGTTGCG
TGATGCCGGCGACCCGGTGGAAATTGCCGCGCGCTACAACGAGCAGGGTGCAGACGAGCTGACTTTTCTGGACATCACGG
CCACCAGCGATGGGCGCGACCTGATTTTGCACATCATCGAGGCGGTGGCCTCACAGGTGTTCATTCCGCTCACCGTGGGC
GGCGGTGTGCGCACGGTGGAAGACGTGCGGCGCCTGCTCAATGCCGGGGCTGACAAGACCAGCTTCAACTCGGCCGCGCT
GGCCAATCCGCAGGTGATTGAAGACGCCTCGGCCAAATACGGCGCGCAGTGCATCGTGGTGGCGATTGACGCCAAGCGAC
GCAGCGAGGAAGACGCCAGAACGCGCGGCGCCGGCTGGGACGTCTACAGCCACGGCGGGCGCAAGAACACCGGGCTGGAC
GCCGTGGCCTGGGCCACCGAGATGGCGCGGCGCGGTGCCGGCGAGATCCTGCTGACCAGCATGGACCGCGACGGCACCAA
GTCGGGGTTTGACCTGGCCCTGACCCGTGCGGTCAGCGACGCCGTCGATGTGCCGGTGATTGCCTCGGGCGGCGTTGGCA
ACCTGGACCATCTGGCCGATGGCATCCAGCTGGGTGGCGCTGACGCGGTGCTGGCGGCCAGCATTTTCCACTATGGTGAA
TACACGGTGCAGCAGGCCAAGCAGCACATGGCCAGGCGCGGCATCCCGGTCAGAATGTGA

Upstream 100 bases:

>100_bases
TATTCCAGCCAGGGCCGCGGAACCGGCTTCGCCGGGCCGCAGGCGCAGCGGCCCCCTCGGGGGGCAGGGCGCCACACGAA
GTGGGCAACCGTGGGGGCTT

Downstream 100 bases:

>100_bases
CGCAGCCCCGCAGGCTTCGCGCACTGCGTGTCGCTACACCTTCCCCCTGCCGGGGCGACATCTGCGGTCCGGCCAAGCCG
GCCCCGCGATGTCCTGGCCT

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MLAKRIIPCLDVTGGRVVKGVNFVELRDAGDPVEIAARYNEQGADELTFLDITATSDGRDLILHIIEAVASQVFIPLTVG
GGVRTVEDVRRLLNAGADKTSFNSAALANPQVIEDASAKYGAQCIVVAIDAKRRSEEDARTRGAGWDVYSHGGRKNTGLD
AVAWATEMARRGAGEILLTSMDRDGTKSGFDLALTRAVSDAVDVPVIASGGVGNLDHLADGIQLGGADAVLAASIFHYGE
YTVQQAKQHMARRGIPVRM

Sequences:

>Translated_259_residues
MLAKRIIPCLDVTGGRVVKGVNFVELRDAGDPVEIAARYNEQGADELTFLDITATSDGRDLILHIIEAVASQVFIPLTVG
GGVRTVEDVRRLLNAGADKTSFNSAALANPQVIEDASAKYGAQCIVVAIDAKRRSEEDARTRGAGWDVYSHGGRKNTGLD
AVAWATEMARRGAGEILLTSMDRDGTKSGFDLALTRAVSDAVDVPVIASGGVGNLDHLADGIQLGGADAVLAASIFHYGE
YTVQQAKQHMARRGIPVRM
>Mature_259_residues
MLAKRIIPCLDVTGGRVVKGVNFVELRDAGDPVEIAARYNEQGADELTFLDITATSDGRDLILHIIEAVASQVFIPLTVG
GGVRTVEDVRRLLNAGADKTSFNSAALANPQVIEDASAKYGAQCIVVAIDAKRRSEEDARTRGAGWDVYSHGGRKNTGLD
AVAWATEMARRGAGEILLTSMDRDGTKSGFDLALTRAVSDAVDVPVIASGGVGNLDHLADGIQLGGADAVLAASIFHYGE
YTVQQAKQHMARRGIPVRM

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=270, Percent_Identity=41.8518518518518, Blast_Score=208, Evalue=3e-55,
Organism=Escherichia coli, GI87082028, Length=245, Percent_Identity=28.9795918367347, Blast_Score=95, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=35.6687898089172, Blast_Score=160, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 27443; Mature: 27443

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDVTGGRVVKGVNFVELRDAGDPVEIAARYNEQGADELTFLDITATSDGRD
CCHHHCCCHHCCCCCEEEECCCEEEECCCCCCEEEHEECCCCCCCCEEEEEEEECCCCHH
LILHIIEAVASQVFIPLTVGGGVRTVEDVRRLLNAGADKTSFNSAALANPQVIEDASAKY
HHHHHHHHHHHCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCCC
GAQCIVVAIDAKRRSEEDARTRGAGWDVYSHGGRKNTGLDAVAWATEMARRGAGEILLTS
CCEEEEEEEECCCCCCHHHHHCCCCCCHHCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEE
MDRDGTKSGFDLALTRAVSDAVDVPVIASGGVGNLDHLADGIQLGGADAVLAASIFHYGE
CCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHH
YTVQQAKQHMARRGIPVRM
HHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLAKRIIPCLDVTGGRVVKGVNFVELRDAGDPVEIAARYNEQGADELTFLDITATSDGRD
CCHHHCCCHHCCCCCEEEECCCEEEECCCCCCEEEHEECCCCCCCCEEEEEEEECCCCHH
LILHIIEAVASQVFIPLTVGGGVRTVEDVRRLLNAGADKTSFNSAALANPQVIEDASAKY
HHHHHHHHHHHCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCCC
GAQCIVVAIDAKRRSEEDARTRGAGWDVYSHGGRKNTGLDAVAWATEMARRGAGEILLTS
CCEEEEEEEECCCCCCHHHHHCCCCCCHHCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEE
MDRDGTKSGFDLALTRAVSDAVDVPVIASGGVGNLDHLADGIQLGGADAVLAASIFHYGE
CCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHH
YTVQQAKQHMARRGIPVRM
HHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA