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The map label for this gene is hisH [H]

Identifier: 91786709

GI number: 91786709

Start: 833192

End: 833863

Strand: Direct

Name: hisH [H]

Synonym: Bpro_0807

Alternate gene names: 91786709

Gene position: 833192-833863 (Clockwise)

Preceding gene: 91786708

Following gene: 91786710

Centisome position: 16.02

GC content: 63.54

Gene sequence:

>672_bases
ATGTCTAATGTGAAAAGAGTGGCGGTCGTCGACTACGGCTCGGGCAACCTGCGTTCGGTGTCACAAGCTGTTCAGCATGT
GTCTCAGGGCAGCGGCTATGAGGTGTTGGTCACCTCGCGCGCGCAGGACGTGCTGGACGCCGAGCGTGTGGTGCTGCCCG
GCCAGGGGGCCATGCCTGACTGCATGCGGGCGCTGTCTGAATCCGGCCTGAAGGATGCGGTATTGCATGCCGCCGCGAAC
AAACCCCTGTTCGGCGTGTGTGTGGGCATGCAGATGCTGCTGGACCGCAGTCACGAGGGGGTCGCCGGCATCGCAACGCC
CGGCCTGGGCCTGATTCCCGGCGAGGTCGTCAAGTTCGACCTGGCCGGGCAGATCCAGCCCGATGGCAGCCGCTACAAGG
TGCCGCAAATGGGCTGGAACCAGGTGTGGCGGCCTGCCTCGATCCAGCACCCGATCTGGGCTGGCGTGCCGGACGGTGCC
TATTTTTACTTCGTGCACAGCTTTTATGCCCGTCCGTCGGATGCGCGCCACATCGCCGGAGAGGCGGACTATGGCGGGCG
CTTTACGGCGGCAATTGCACGCGATAATATTTTTGCCACCCAGTTCCACCCCGAGAAAAGCGCCGACCACGGACTGGCCC
TGTACCGCAATTTCCTGCACTGGAAGCCCTGA

Upstream 100 bases:

>100_bases
AGGTGCCGGTGCGCCATGGATGGACCGTTCCCCGTTTTGGGTTTTCTGCCCCGCTAGAATGGACGTCAATAGCTCCTGAG
TCCGTAGCAAATATCGATGA

Downstream 100 bases:

>100_bases
AGGCCGCCGGGCAGCAGGGCGACTCATCATAATTTCCAGCTCTTTGTTAACCCCGCCACCTGTTTTCCTCTTACCGCAAC
ATGCTACTTATTCCCGCGAT

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 223; Mature: 222

Protein sequence:

>223_residues
MSNVKRVAVVDYGSGNLRSVSQAVQHVSQGSGYEVLVTSRAQDVLDAERVVLPGQGAMPDCMRALSESGLKDAVLHAAAN
KPLFGVCVGMQMLLDRSHEGVAGIATPGLGLIPGEVVKFDLAGQIQPDGSRYKVPQMGWNQVWRPASIQHPIWAGVPDGA
YFYFVHSFYARPSDARHIAGEADYGGRFTAAIARDNIFATQFHPEKSADHGLALYRNFLHWKP

Sequences:

>Translated_223_residues
MSNVKRVAVVDYGSGNLRSVSQAVQHVSQGSGYEVLVTSRAQDVLDAERVVLPGQGAMPDCMRALSESGLKDAVLHAAAN
KPLFGVCVGMQMLLDRSHEGVAGIATPGLGLIPGEVVKFDLAGQIQPDGSRYKVPQMGWNQVWRPASIQHPIWAGVPDGA
YFYFVHSFYARPSDARHIAGEADYGGRFTAAIARDNIFATQFHPEKSADHGLALYRNFLHWKP
>Mature_222_residues
SNVKRVAVVDYGSGNLRSVSQAVQHVSQGSGYEVLVTSRAQDVLDAERVVLPGQGAMPDCMRALSESGLKDAVLHAAANK
PLFGVCVGMQMLLDRSHEGVAGIATPGLGLIPGEVVKFDLAGQIQPDGSRYKVPQMGWNQVWRPASIQHPIWAGVPDGAY
FYFVHSFYARPSDARHIAGEADYGGRFTAAIARDNIFATQFHPEKSADHGLALYRNFLHWKP

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=215, Percent_Identity=38.6046511627907, Blast_Score=125, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6319725, Length=225, Percent_Identity=33.3333333333333, Blast_Score=105, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 24201; Mature: 24070

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNVKRVAVVDYGSGNLRSVSQAVQHVSQGSGYEVLVTSRAQDVLDAERVVLPGQGAMPD
CCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHCCCEEEECCCCCCHH
CMRALSESGLKDAVLHAAANKPLFGVCVGMQMLLDRSHEGVAGIATPGLGLIPGEVVKFD
HHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCEEEEE
LAGQIQPDGSRYKVPQMGWNQVWRPASIQHPIWAGVPDGAYFYFVHSFYARPSDARHIAG
ECCEECCCCCEEECCCCCHHHCCCCCCCCCCCCCCCCCCHHHEEHHHHHCCCCCCCCCCC
EADYGGRFTAAIARDNIFATQFHPEKSADHGLALYRNFLHWKP
CCCCCCEEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
SNVKRVAVVDYGSGNLRSVSQAVQHVSQGSGYEVLVTSRAQDVLDAERVVLPGQGAMPD
CCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHCCCEEEECCCCCCHH
CMRALSESGLKDAVLHAAANKPLFGVCVGMQMLLDRSHEGVAGIATPGLGLIPGEVVKFD
HHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCEEEEE
LAGQIQPDGSRYKVPQMGWNQVWRPASIQHPIWAGVPDGAYFYFVHSFYARPSDARHIAG
ECCEECCCCCEEECCCCCHHHCCCCCCCCCCCCCCCCCCHHHEEHHHHHCCCCCCCCCCC
EADYGGRFTAAIARDNIFATQFHPEKSADHGLALYRNFLHWKP
CCCCCCEEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]