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The map label for this gene is pdhC [H]

Identifier: 91786666

GI number: 91786666

Start: 784312

End: 785574

Strand: Direct

Name: pdhC [H]

Synonym: Bpro_0764

Alternate gene names: 91786666

Gene position: 784312-785574 (Clockwise)

Preceding gene: 91786665

Following gene: 91786667

Centisome position: 15.08

GC content: 68.17

Gene sequence:

>1263_bases
ATGATCGAATTCAAGTTGCCCGCGCTGGGCGCTGACATGGACGAGGGCACACTGCTCAAGTGGCATGTGCAACCGGGAGA
CGCCGTCAAGCGTGGCCAGGTGGTGGCTGTGGTGGACACCTCCAAGGCCGCGGTGGATGTTGAAATCTGGCAGGACGGGG
TGATGACCGAGTTGCGGGTGCAGCCCGGCGAGAAGGTGCCGGTGGGTACGGTGCTGGCCAGGCTGCTGGCGCCGGGAGAG
GTGGCGGCGCCTGCGGCCACTTCTCTCACTGCTTCCACTGTTCCCACCGTTCCGGTCGCCGTACCGCAGTCCGTGCCCGC
AGCCCCGCCGCAGCCTGCGTTACCACGTGTGCCGGAGATCGAAAAAGCACTTCCTGCGGCACGGCATCCGGTGTCGCCTT
CGGCACGCCGGCACGCCAGGGAACATGGCATTGACCCCGACAGTGTGCATGGCACCGGTCCGCAAGGCTCGGTCACGCTG
GCCGATATCGAAGCCGCCGTGGCTGCGCGCAGGCCACCTTTGCCGGTGGCGGCGGCGCCAGACCGGCAGACGGAAATGCG
CAAGGCCATTGCCGCGACCATGAGCCGCTCCAAGCGCGAGATTCCGCACTATTACCTCAGCGAGCCCATTCCCATGGCCA
GGGCGCTGGCCTGGCTGCTGCAGCGCAACGAAGGCCTGCCGATCACCGAACGTATCCTGCCTGCGGTGTTGCAGCTCAAG
GCTGTGGCTGCGGCCCTGCAGCGCGTGCCTCAGCTCAACGGGTTGTATCGCGACGGCGCGTTCCAGCTGTCCACGGCCGT
GCACGCCGGTGTGGCGATCTCCCTGCGCGGCGGCGGCCTGGTGGCGCCGGCGATCCATGATGTTGGCGCCAAACCGCTGG
AGCTGCTCATGCGCGAGCTGGCCGATCTGGTCAAACGCGCGCGAGCCGGTTCCTTGCGCAGCTCCGAGATGAGCGACCCG
ACCATCACCATCACCAACCTGGGCGACCAGGGCGTGGAAGCCGTCTTTGGCGTGATCTATCCGCCGCAGGTGGCGCTGGT
GGGTTTTGGCCGCGTGGCAGATGGCCCCTGGGTGGAGGAGGGGGAAGTTCGCGCGCTGCCGCTGGTCACGGCCACGCTGG
CCGCCGATCACCGGGTGTCCGACGGGCACCAGGGCGCGCTGTTTCTGGCCGAGCTGCGCGATCTTCTGCAGCAGCCTCAG
GCGCTGGCTGAACGAGTCACGCCAGCCCCCGGCCAGGGCGGCCAGGCAGGAGGCCCGACATGA

Upstream 100 bases:

>100_bases
AGGAGGTTCCGATTCCGTATGCCCGCCACATGGAAGAGGCCGCGTTGCCGCAAGTCCCGAAAATTGTCGCCGCCGCGCGA
GCGCTGTTGGGGAGCGCACC

Downstream 100 bases:

>100_bases
GCTATGACCGTCCGGCGTTGAGCCGCATCGTCCTGGATATCCTGTGCTCGATTGCACCAGAGGTCGTTCCCGCCACCATT
GAACCCGACCGCCCGCTGCG

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MIEFKLPALGADMDEGTLLKWHVQPGDAVKRGQVVAVVDTSKAAVDVEIWQDGVMTELRVQPGEKVPVGTVLARLLAPGE
VAAPAATSLTASTVPTVPVAVPQSVPAAPPQPALPRVPEIEKALPAARHPVSPSARRHAREHGIDPDSVHGTGPQGSVTL
ADIEAAVAARRPPLPVAAAPDRQTEMRKAIAATMSRSKREIPHYYLSEPIPMARALAWLLQRNEGLPITERILPAVLQLK
AVAAALQRVPQLNGLYRDGAFQLSTAVHAGVAISLRGGGLVAPAIHDVGAKPLELLMRELADLVKRARAGSLRSSEMSDP
TITITNLGDQGVEAVFGVIYPPQVALVGFGRVADGPWVEEGEVRALPLVTATLAADHRVSDGHQGALFLAELRDLLQQPQ
ALAERVTPAPGQGGQAGGPT

Sequences:

>Translated_420_residues
MIEFKLPALGADMDEGTLLKWHVQPGDAVKRGQVVAVVDTSKAAVDVEIWQDGVMTELRVQPGEKVPVGTVLARLLAPGE
VAAPAATSLTASTVPTVPVAVPQSVPAAPPQPALPRVPEIEKALPAARHPVSPSARRHAREHGIDPDSVHGTGPQGSVTL
ADIEAAVAARRPPLPVAAAPDRQTEMRKAIAATMSRSKREIPHYYLSEPIPMARALAWLLQRNEGLPITERILPAVLQLK
AVAAALQRVPQLNGLYRDGAFQLSTAVHAGVAISLRGGGLVAPAIHDVGAKPLELLMRELADLVKRARAGSLRSSEMSDP
TITITNLGDQGVEAVFGVIYPPQVALVGFGRVADGPWVEEGEVRALPLVTATLAADHRVSDGHQGALFLAELRDLLQQPQ
ALAERVTPAPGQGGQAGGPT
>Mature_420_residues
MIEFKLPALGADMDEGTLLKWHVQPGDAVKRGQVVAVVDTSKAAVDVEIWQDGVMTELRVQPGEKVPVGTVLARLLAPGE
VAAPAATSLTASTVPTVPVAVPQSVPAAPPQPALPRVPEIEKALPAARHPVSPSARRHAREHGIDPDSVHGTGPQGSVTL
ADIEAAVAARRPPLPVAAAPDRQTEMRKAIAATMSRSKREIPHYYLSEPIPMARALAWLLQRNEGLPITERILPAVLQLK
AVAAALQRVPQLNGLYRDGAFQLSTAVHAGVAISLRGGGLVAPAIHDVGAKPLELLMRELADLVKRARAGSLRSSEMSDP
TITITNLGDQGVEAVFGVIYPPQVALVGFGRVADGPWVEEGEVRALPLVTATLAADHRVSDGHQGALFLAELRDLLQQPQ
ALAERVTPAPGQGGQAGGPT

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=433, Percent_Identity=31.1778290993072, Blast_Score=169, Evalue=7e-42,
Organism=Homo sapiens, GI203098816, Length=467, Percent_Identity=24.4111349036403, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI203098753, Length=467, Percent_Identity=24.4111349036403, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI110671329, Length=439, Percent_Identity=23.9179954441913, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI19923748, Length=227, Percent_Identity=25.5506607929515, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1786305, Length=429, Percent_Identity=27.972027972028, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1786946, Length=427, Percent_Identity=24.5901639344262, Blast_Score=118, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI17560088, Length=430, Percent_Identity=30.9302325581395, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI25146366, Length=412, Percent_Identity=28.6407766990291, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324258, Length=441, Percent_Identity=27.891156462585, Blast_Score=140, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6320352, Length=414, Percent_Identity=25.1207729468599, Blast_Score=97, Evalue=3e-21,
Organism=Drosophila melanogaster, GI18859875, Length=435, Percent_Identity=26.4367816091954, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24645909, Length=227, Percent_Identity=27.3127753303965, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 44087; Mature: 44087

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS50968 BIOTINYL_LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEFKLPALGADMDEGTLLKWHVQPGDAVKRGQVVAVVDTSKAAVDVEIWQDGVMTELRV
CCEEECCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCEEEEEECCCCCEEEEEE
QPGEKVPVGTVLARLLAPGEVAAPAATSLTASTVPTVPVAVPQSVPAAPPQPALPRVPEI
CCCCCCCHHHHHHHHHCCCCCCCCCHHCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCH
EKALPAARHPVSPSARRHAREHGIDPDSVHGTGPQGSVTLADIEAAVAARRPPLPVAAAP
HHHCCHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEHHHHHHHHHHCCCCCCCCCCC
DRQTEMRKAIAATMSRSKREIPHYYLSEPIPMARALAWLLQRNEGLPITERILPAVLQLK
CHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
AVAAALQRVPQLNGLYRDGAFQLSTAVHAGVAISLRGGGLVAPAIHDVGAKPLELLMREL
HHHHHHHHCHHHCCEECCCCHHHHHHHHCCEEEEECCCCEECCHHHHCCCCHHHHHHHHH
ADLVKRARAGSLRSSEMSDPTITITNLGDQGVEAVFGVIYPPQVALVGFGRVADGPWVEE
HHHHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCC
GEVRALPLVTATLAADHRVSDGHQGALFLAELRDLLQQPQALAERVTPAPGQGGQAGGPT
CCEEEEHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIEFKLPALGADMDEGTLLKWHVQPGDAVKRGQVVAVVDTSKAAVDVEIWQDGVMTELRV
CCEEECCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCEEEEEECCCCCEEEEEE
QPGEKVPVGTVLARLLAPGEVAAPAATSLTASTVPTVPVAVPQSVPAAPPQPALPRVPEI
CCCCCCCHHHHHHHHHCCCCCCCCCHHCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCH
EKALPAARHPVSPSARRHAREHGIDPDSVHGTGPQGSVTLADIEAAVAARRPPLPVAAAP
HHHCCHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEHHHHHHHHHHCCCCCCCCCCC
DRQTEMRKAIAATMSRSKREIPHYYLSEPIPMARALAWLLQRNEGLPITERILPAVLQLK
CHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
AVAAALQRVPQLNGLYRDGAFQLSTAVHAGVAISLRGGGLVAPAIHDVGAKPLELLMREL
HHHHHHHHCHHHCCEECCCCHHHHHHHHCCEEEEECCCCEECCHHHHCCCCHHHHHHHHH
ADLVKRARAGSLRSSEMSDPTITITNLGDQGVEAVFGVIYPPQVALVGFGRVADGPWVEE
HHHHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCC
GEVRALPLVTATLAADHRVSDGHQGALFLAELRDLLQQPQALAERVTPAPGQGGQAGGPT
CCEEEEHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]