Definition Escherichia coli UTI89 plasmid pUTI89, complete sequence.
Accession NC_007941
Length 114,230

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The map label for this gene is traF [H]

Identifier: 91206370

GI number: 91206370

Start: 93687

End: 94460

Strand: Direct

Name: traF [H]

Synonym: UTI89_P125

Alternate gene names: 91206370

Gene position: 93687-94460 (Clockwise)

Preceding gene: 91206369

Following gene: 91206371

Centisome position: 82.02

GC content: 51.29

Gene sequence:

>774_bases
ATGAAAGAAAAAGTGATGAAAGGAATGAAGATGAATAAAGCATTACTGCCACTGTTACTCTGCTGCTTTATTTTTCCGGC
GTCAGGAAAAGATGCAGGCTGGCAGTGGTATAACGAGAAAATAAATCCGAAGGAAAAAGAAAATAAACCAGTACCTGCAG
CCCCCCGTCAGGAGCCGGACATTATGCAGAAACTGACCGCGCTGCAGACGGCAACAAAGCGGGCGCTGTACGAAGCTATT
CTGTATCCCGGCGTGGATAATTTTGTGAAATATTTCCGGCTGCAGAATTACTGGACTCAGCAGGCCGGGCTTTTCACCAT
GAGCGCCAAAAAAGCCATGCTGGCGCATCCCGAACTGGACTATAACCTGCAGTACAGCCATTACAACGGCACGGTCCGGA
ACCAGCTGGCGGCAGACCAGGCGCAGCAGCGACAGGCCATTGCGAAACTGGCTGAACACTACGGCATCATGTTCTTTTAC
CGGGGGCAGGACCCCATCGACGGGCAGCTGGCGCAGGTCATTAATGGCTTCAGGGATACCTATGGTCTGAGTGTTATTCC
CGTTTCCGTGGATGGCGTGATTAATCCGCTGTTGCCGGATTCCCGGACTGACCAGGGGCAGGCGCAGCGCCTCGGTGTGA
AATATTTCCCGGCCATGATGCTGGTTGACCCGAAACAGGGCAGTGTTCGCCCGTTATCATACGGCTTTATTTCACAGGAC
GACCTGGCAAAACAGTTCCTGAACGTTTCTGAAGATTTTAAACCGAATTTTTAA

Upstream 100 bases:

>100_bases
GACGGGATACTTGCTGCTGCTTTCGTTATCCTTTACTCCGGTGTGCTTCTGTTTATTCTGTATTTTTGTTTTTCTGCCCT
GACTGACCTGCAGAAACCTG

Downstream 100 bases:

>100_bases
CCAAAGGTTATATATGCATACAGGGGATTTGTGTTTTTTTACAGGTGCCAGCCTGTTTATCGTGGGGGTTGTTGGTTATT
TCTTTACAGGACCAAATAAT

Product: conjugal pilus assembly protein TraF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MKEKVMKGMKMNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAAPRQEPDIMQKLTALQTATKRALYEAI
LYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKAMLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFY
RGQDPIDGQLAQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGSVRPLSYGFISQD
DLAKQFLNVSEDFKPNF

Sequences:

>Translated_257_residues
MKEKVMKGMKMNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAAPRQEPDIMQKLTALQTATKRALYEAI
LYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKAMLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFY
RGQDPIDGQLAQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGSVRPLSYGFISQD
DLAKQFLNVSEDFKPNF
>Mature_257_residues
MKEKVMKGMKMNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAAPRQEPDIMQKLTALQTATKRALYEAI
LYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKAMLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFY
RGQDPIDGQLAQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGSVRPLSYGFISQD
DLAKQFLNVSEDFKPNF

Specific function: Involved in F pilus assembly [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014110 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 29197; Mature: 29197

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEKVMKGMKMNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAAPRQEPD
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCHHCCCCCCCCCCCCHH
IMQKLTALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKAMLAHPELD
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHCCCCCC
YNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQLAQVINGFRDT
CEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHH
YGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGSVRPLSYGFISQD
CCCEEEEEECCCHHCCCCCCCCCCCCHHHHCCHHHCCEEEEECCCCCCCCCEECCCCCHH
DLAKQFLNVSEDFKPNF
HHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKEKVMKGMKMNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAAPRQEPD
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCHHCCCCCCCCCCCCHH
IMQKLTALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKAMLAHPELD
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHCCCCCC
YNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQLAQVINGFRDT
CEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHH
YGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGSVRPLSYGFISQD
CCCEEEEEECCCHHCCCCCCCCCCCCHHHHCCHHHCCEEEEECCCCCCCCCEECCCCCHH
DLAKQFLNVSEDFKPNF
HHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3042757; 7915817; 1593622 [H]