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The map label for this gene is zraS [H]

Identifier: 89885899

GI number: 89885899

Start: 179481

End: 181178

Strand: Reverse

Name: zraS [H]

Synonym: Rfer_4413

Alternate gene names: 89885899

Gene position: 181178-179481 (Counterclockwise)

Preceding gene: 89885900

Following gene: 89885898

Centisome position: 70.37

GC content: 50.41

Gene sequence:

>1698_bases
ATGAATAAGCAAGATAAAAAATGGAGAGTCCTTGTCGTTGACGACGATGCGGATGTTCTGACAAACACCGTCTACACCCT
GATGGATCTCAAGGTTTTAGGGCGTGAGTTGGATATAACCACGCTGGGCAGTGCTACGGCAGCGATTGAGTTGCTTGAAA
GGGAGCGTGATTTCGCTGTAATTTTGTTGGATGTTGTCATGGAGAAACCCCACGCGGGATTGAGCCTGGTGACCATGATT
CGTCATGATTTCAATATGCAAATGTCTCGGATCATTTTGAGGACGGGTAACCCCGGATACGCCCCAGAACGAGATATAAC
CCTGAAGCTGGAAATCGACGACTACATCCTTAAGCAAACAGCCGTTCGTGATCGGATAATCACGGCAGTGATTGTTGCCA
TCAGAGCCTATGATCAACTACAGCGATTGGCGCGATTGAAGGACGCCATGCAGCAAATAATTTGTTGTTCGAATCAGCTT
TTGACTACAGACGAAATCGACCAGTTTGCAAACGTCTGTATGCAGTACGTTGCGCCTATTTTTGGAAAGAATGCCGGTGG
GGGACTGGCCTACGCCGGTATGACAGTAAATCAAGGGATAGTGCCCCTGACCGTGATGTCGGCAACCAGCGAGCATGAGT
CTCTCATTGAACCGTTGAATGGAATCGGCGTTTTGCCTCCCAATATTTCAGTGACCAAGAAGAAGATGACCAAGGCGTTG
GAGCAGACGAATAGCATTAAGACCGACAAAGATTGGTACATCTACATCAAAGTTTCACTCGGGCGCAAGGTGGTGCTTTG
GTTGAACAAGCCTGACGGGTTTGATGAAATCGACGAGACACTCGGGAAGTCCTTTGCCAACACCATCAAAGCATGCTTAG
ATCGGCTGTGCTTGCTCCGGGAACGGATGGCTGAGGCGATGGTATCGATTGGAATTTTCGCTCATGAATTCAGGACCCCT
ATCGCATCCATGCGAATGAGCAATGAATTCATGCTCGAATCGCTTGAAGCCGGCGAATGCGACAAAGAGCGGTTCAAAAT
ATTACTCAACAATACCGACCGGATTCTGAACGGCATGAACAGGCACATCGATTCGTCAATGATAAATGTTGGCGTCGTGC
TGAGAGAGCGGCTGGAGTTGCCTGTGGCCAGGGTAAACATTGGGCCGATCGTCAGGGAGTCGTTGGAGGTCAACAAGAAT
TGGTTTTCAAAAGCAGGGCAAATGCGAGTCCACATTGAAGACGATTGTTGGGCCCGAGCAGATGGCACCATGTTCGAATA
TGCATTCGTCAATCTGGTGGGCAACGCCATCAAGGCGCTATCTAACAGCACAACGCATTTCGATGGGCCGAAAATTGAAA
TTGAATTGACTAAACAAGACGGTCAAATCCGGTTGGTCATTGCGGACCATGGCTCCGGGATTCGCGCGGACCAACTCGAC
AAAATATTCGATCCGTTTTATTCCACCAGTGGGACACCGGCGCATGGTCTTGGGTTGACAATGGTGAAAAGGGCGGTGCA
GGCCATGGGTGGAACCATCGAATGCAGTTCCGAGGTTGGGGTTGGGACAACCTTTGTCATTCAATTGAACGCCTTTGACG
TGCCACCACCGGAACCGTTGAACCATGTCGGCCGGCTGGAGAGGCGATTGTCAGAGCGCCCAGTTGTCGCCCGGCACGCG
CCTGCTCAGCCCGGCTGA

Upstream 100 bases:

>100_bases
CGGGACCCGACGGATCCAAATTCATCGTCACGATTCCATTGAGTTCGCCCACTGTCGAAAACGATAGTTCCCCGCCAGAC
ACAGTTCCGGGTTTTCTTCA

Downstream 100 bases:

>100_bases
TTTAGTGCAAAAAACAGTGTTACCCACTCGAAATTGTGTTAAAGTGATATTTACTCAGCGCTAAGCTGTTAGTCGTTCGT
CAGGAACGGCATGTCAAACC

Product: response regulator receiver sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 565; Mature: 565

Protein sequence:

>565_residues
MNKQDKKWRVLVVDDDADVLTNTVYTLMDLKVLGRELDITTLGSATAAIELLERERDFAVILLDVVMEKPHAGLSLVTMI
RHDFNMQMSRIILRTGNPGYAPERDITLKLEIDDYILKQTAVRDRIITAVIVAIRAYDQLQRLARLKDAMQQIICCSNQL
LTTDEIDQFANVCMQYVAPIFGKNAGGGLAYAGMTVNQGIVPLTVMSATSEHESLIEPLNGIGVLPPNISVTKKKMTKAL
EQTNSIKTDKDWYIYIKVSLGRKVVLWLNKPDGFDEIDETLGKSFANTIKACLDRLCLLRERMAEAMVSIGIFAHEFRTP
IASMRMSNEFMLESLEAGECDKERFKILLNNTDRILNGMNRHIDSSMINVGVVLRERLELPVARVNIGPIVRESLEVNKN
WFSKAGQMRVHIEDDCWARADGTMFEYAFVNLVGNAIKALSNSTTHFDGPKIEIELTKQDGQIRLVIADHGSGIRADQLD
KIFDPFYSTSGTPAHGLGLTMVKRAVQAMGGTIECSSEVGVGTTFVIQLNAFDVPPPEPLNHVGRLERRLSERPVVARHA
PAQPG

Sequences:

>Translated_565_residues
MNKQDKKWRVLVVDDDADVLTNTVYTLMDLKVLGRELDITTLGSATAAIELLERERDFAVILLDVVMEKPHAGLSLVTMI
RHDFNMQMSRIILRTGNPGYAPERDITLKLEIDDYILKQTAVRDRIITAVIVAIRAYDQLQRLARLKDAMQQIICCSNQL
LTTDEIDQFANVCMQYVAPIFGKNAGGGLAYAGMTVNQGIVPLTVMSATSEHESLIEPLNGIGVLPPNISVTKKKMTKAL
EQTNSIKTDKDWYIYIKVSLGRKVVLWLNKPDGFDEIDETLGKSFANTIKACLDRLCLLRERMAEAMVSIGIFAHEFRTP
IASMRMSNEFMLESLEAGECDKERFKILLNNTDRILNGMNRHIDSSMINVGVVLRERLELPVARVNIGPIVRESLEVNKN
WFSKAGQMRVHIEDDCWARADGTMFEYAFVNLVGNAIKALSNSTTHFDGPKIEIELTKQDGQIRLVIADHGSGIRADQLD
KIFDPFYSTSGTPAHGLGLTMVKRAVQAMGGTIECSSEVGVGTTFVIQLNAFDVPPPEPLNHVGRLERRLSERPVVARHA
PAQPG
>Mature_565_residues
MNKQDKKWRVLVVDDDADVLTNTVYTLMDLKVLGRELDITTLGSATAAIELLERERDFAVILLDVVMEKPHAGLSLVTMI
RHDFNMQMSRIILRTGNPGYAPERDITLKLEIDDYILKQTAVRDRIITAVIVAIRAYDQLQRLARLKDAMQQIICCSNQL
LTTDEIDQFANVCMQYVAPIFGKNAGGGLAYAGMTVNQGIVPLTVMSATSEHESLIEPLNGIGVLPPNISVTKKKMTKAL
EQTNSIKTDKDWYIYIKVSLGRKVVLWLNKPDGFDEIDETLGKSFANTIKACLDRLCLLRERMAEAMVSIGIFAHEFRTP
IASMRMSNEFMLESLEAGECDKERFKILLNNTDRILNGMNRHIDSSMINVGVVLRERLELPVARVNIGPIVRESLEVNKN
WFSKAGQMRVHIEDDCWARADGTMFEYAFVNLVGNAIKALSNSTTHFDGPKIEIELTKQDGQIRLVIADHGSGIRADQLD
KIFDPFYSTSGTPAHGLGLTMVKRAVQAMGGTIECSSEVGVGTTFVIQLNAFDVPPPEPLNHVGRLERRLSERPVVARHA
PAQPG

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=240, Percent_Identity=28.3333333333333, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI87081816, Length=258, Percent_Identity=25.5813953488372, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1790567, Length=99, Percent_Identity=37.3737373737374, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 62967; Mature: 62967

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKQDKKWRVLVVDDDADVLTNTVYTLMDLKVLGRELDITTLGSATAAIELLERERDFAV
CCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHCCCCCEE
ILLDVVMEKPHAGLSLVTMIRHDFNMQMSRIILRTGNPGYAPERDITLKLEIDDYILKQT
EEEHHHHCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCEEEEEEEHHHHHHHH
AVRDRIITAVIVAIRAYDQLQRLARLKDAMQQIICCSNQLLTTDEIDQFANVCMQYVAPI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
FGKNAGGGLAYAGMTVNQGIVPLTVMSATSEHESLIEPLNGIGVLPPNISVTKKKMTKAL
HCCCCCCCEEEECEEECCCCEEEEEEECCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
EQTNSIKTDKDWYIYIKVSLGRKVVLWLNKPDGFDEIDETLGKSFANTIKACLDRLCLLR
HHHCCCCCCCCEEEEEEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERMAEAMVSIGIFAHEFRTPIASMRMSNEFMLESLEAGECDKERFKILLNNTDRILNGMN
HHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHCCCCCCCHHHHEEEECCHHHHHHHHH
RHIDSSMINVGVVLRERLELPVARVNIGPIVRESLEVNKNWFSKAGQMRVHIEDDCWARA
HHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHCHHHHHCCCCEEEEECCCCEECC
DGTMFEYAFVNLVGNAIKALSNSTTHFDGPKIEIELTKQDGQIRLVIADHGSGIRADQLD
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCEEEEEEEECCCCCCHHHHH
KIFDPFYSTSGTPAHGLGLTMVKRAVQAMGGTIECSSEVGVGTTFVIQLNAFDVPPPEPL
HHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCCEEEEEEEECCCCCCCCHH
NHVGRLERRLSERPVVARHAPAQPG
HHHHHHHHHHHCCCCEEECCCCCCC
>Mature Secondary Structure
MNKQDKKWRVLVVDDDADVLTNTVYTLMDLKVLGRELDITTLGSATAAIELLERERDFAV
CCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHCCCCCEE
ILLDVVMEKPHAGLSLVTMIRHDFNMQMSRIILRTGNPGYAPERDITLKLEIDDYILKQT
EEEHHHHCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCEEEEEEEHHHHHHHH
AVRDRIITAVIVAIRAYDQLQRLARLKDAMQQIICCSNQLLTTDEIDQFANVCMQYVAPI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
FGKNAGGGLAYAGMTVNQGIVPLTVMSATSEHESLIEPLNGIGVLPPNISVTKKKMTKAL
HCCCCCCCEEEECEEECCCCEEEEEEECCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
EQTNSIKTDKDWYIYIKVSLGRKVVLWLNKPDGFDEIDETLGKSFANTIKACLDRLCLLR
HHHCCCCCCCCEEEEEEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERMAEAMVSIGIFAHEFRTPIASMRMSNEFMLESLEAGECDKERFKILLNNTDRILNGMN
HHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHCCCCCCCHHHHEEEECCHHHHHHHHH
RHIDSSMINVGVVLRERLELPVARVNIGPIVRESLEVNKNWFSKAGQMRVHIEDDCWARA
HHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHCHHHHHCCCCEEEEECCCCEECC
DGTMFEYAFVNLVGNAIKALSNSTTHFDGPKIEIELTKQDGQIRLVIADHGSGIRADQLD
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCEEEEEEEECCCCCCHHHHH
KIFDPFYSTSGTPAHGLGLTMVKRAVQAMGGTIECSSEVGVGTTFVIQLNAFDVPPPEPL
HHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCCEEEEEEEECCCCCCCCHH
NHVGRLERRLSERPVVARHAPAQPG
HHHHHHHHHHHCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 2666400; 11243806 [H]