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The map label for this gene is spoT [H]

Identifier: 89256706

GI number: 89256706

Start: 1340914

End: 1343028

Strand: Reverse

Name: spoT [H]

Synonym: FTL_1413

Alternate gene names: 89256706

Gene position: 1343028-1340914 (Counterclockwise)

Preceding gene: 89256707

Following gene: 89256702

Centisome position: 70.84

GC content: 32.58

Gene sequence:

>2115_bases
ATGTTTTGTTTCTATGACCTTAATCAATTAATTTCTCAATACCTTCCTCATCGAGAAAGGCTAAAGATAGCTCAAGCATT
TATATTTGGTGCTGATGCTCACGAAACTCAAGTTAGAAGCTCAGGGGAGCCTTATTTTACCCATCCTGTTGCTGTAGCAT
GTATACTTGCTGAGCTTAGGATGGATGTTGATACAATAATTGCAGCTTTATTGCATGATGTCGTAGAGGATACTGAGTAT
ACAGTTGACGATATTAGTAATATTTTTGGTAAAAAAGTTGCTCAACTTGTCGAAGGAGTAACTAAACTTACTCAAATTAG
GCATAAAAACCGAGCGGAACAACAAGCAGAAAACTTCCGCAAAATGTTACTATCTGTGACAAAAGATGTTCGTGTAATAT
TTATTAAGCTAGCTGATAGGCTTCATAATATGCGTACATTAGCACCACTAAAACCTGAGAAAAAGCGTCGAATTTCTAAA
GAAACTTTAGATGTGTTTGCACCTCTTGCTCATCGACTAGGTATCAATACTCTTAAAGAACAGTTGGAAACCCTTGCGTT
TGAGGGTATATATCCTTATCGTTATCATATTTTAGAAGAAAAAGTAAAAAAAGTAGAAAAAAATAAAGAAAAAGTCTTTT
ACGAGGTAAAAGAAGCATTAGCAGAAAAGCTTAATGACTTAGTCTCATTAGAAGATATCAAAGCACGTAAGAAAACTTTG
TACAGTATATATAACAAAATGCGTAAAAAGGGTATATCTTTTGATGAAATTATGGACATGTACGCTTACAAAATTATTGT
TCCTAATAGAATAGATTGTTATGTTGCTCTTGGTAAGGTTCATGAGTTATATAAGCCAATACCACAAAAATTCAAAGATT
ATATTGCAACACCAAAAGCAAATGGTTATCGTTCATTACATACGGTTGTCTTAGGGCCGTACAATATTCCATTAGAGATT
CAAATAAAAACAGAACAAATGGATCGTCAAGCCGAATATGGTATTGCAGCTCATTGGAGTTATAAGATCGGTGAGAAAAC
TGACAAAGCATTACAAAGATGGCTTAAGAAAATTTCTGATATTAATGTGTATACTGCGAGCTCTGTAGAGTTTTTAGAAA
ATGTCAAAACAGATATTTTTAATAATGATGTGTTTGTATTTACTCCTCAAGGTGAGATTGTTGAATTACCTATGAATTCA
ACTTGTATAGATTTTGCCTACTATATACATACTGATATTGGTAATAAATGTATTTCAGCTAAAGTAAACCGTAAGTCTGT
TCCACTTAACTACAGATTAAAGCAGGGTGATAATGTTGAAATTATTACTTCAGCAATTGCTGATCCTAATCCGGCATGGT
TAAAATTTGTTGAAACTAATAGAGCTAAATCAGCTATCAAGGACTTCTTGAAACAGCAATATCGTAATATTGATTATATT
CGTGGCAAAGATATAGTCGAAGGTGAACTTAGACTTTTAGGTGTTGAACTAAGGGATGTTCCAAGTGAAATTATAGATGG
AGCATTATTTAATTATGAAGGAATAGATACAATAAATCGCTTCTATCTAGATACTGGCTTAGGTCTTATTGATCCAAATA
ACTTTATTGATTTCGTTGTTAAGCATTTTGAGGATATGCGTAACGCTTATAAAAAATCATCTTTATCAAAATTACAAATT
AGATATGGTGATGATCCACGGATTGCTGATTGTTGTTTACCTCTGCCAAATGATGAGATAGTTGGTATTGTTAACGAGCA
TGGCAAAGTTGAGGTCCATCGAAAAAGCTGTAATGAGTTGTATACAAAAATTAAGGATAATCAAACTAAAGAGATACAGG
CAGATTGGTTTACAAATAGTGATGATGATCCTAGCTTTAAAGCTAGACTTGCAGTTACACTCAAAAATATTCCAGGTTCG
ATAGCTAAAATTACAGCAACTTTAGCTCGAGAAGGTGTTGATATTAGAAGTTTTGATATGATATCTGTAGATAACAAACA
AGCTAAACTGTCATGTGTTGTAGTAGTTAGAAATAGAAGAGAACTATATTTATTGGTTCGTTTAGTAAGAAAGTTAGATG
TTTGTTTAAATATCGAAAGAATATTAAATAAGTAA

Upstream 100 bases:

>100_bases
GAAAATATCTAATTTCTTTCAAAACAAAGTAGTTATAAATTGCTTTGATTTATGATTCAATTTATCATTTAGTTAAGTGT
TATAAAATATTTTGCTAAAT

Downstream 100 bases:

>100_bases
ATTATTATTAAATATTAGTTCTTGTATTAAAAGCATGTAGTAAAGTTTGTTGATCCAAGTATTCTAATTCACCACCAAAT
GGTACTCCAAAACCGATGCG

Product: guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase/(p)ppGpp synthase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 704; Mature: 704

Protein sequence:

>704_residues
MFCFYDLNQLISQYLPHRERLKIAQAFIFGADAHETQVRSSGEPYFTHPVAVACILAELRMDVDTIIAALLHDVVEDTEY
TVDDISNIFGKKVAQLVEGVTKLTQIRHKNRAEQQAENFRKMLLSVTKDVRVIFIKLADRLHNMRTLAPLKPEKKRRISK
ETLDVFAPLAHRLGINTLKEQLETLAFEGIYPYRYHILEEKVKKVEKNKEKVFYEVKEALAEKLNDLVSLEDIKARKKTL
YSIYNKMRKKGISFDEIMDMYAYKIIVPNRIDCYVALGKVHELYKPIPQKFKDYIATPKANGYRSLHTVVLGPYNIPLEI
QIKTEQMDRQAEYGIAAHWSYKIGEKTDKALQRWLKKISDINVYTASSVEFLENVKTDIFNNDVFVFTPQGEIVELPMNS
TCIDFAYYIHTDIGNKCISAKVNRKSVPLNYRLKQGDNVEIITSAIADPNPAWLKFVETNRAKSAIKDFLKQQYRNIDYI
RGKDIVEGELRLLGVELRDVPSEIIDGALFNYEGIDTINRFYLDTGLGLIDPNNFIDFVVKHFEDMRNAYKKSSLSKLQI
RYGDDPRIADCCLPLPNDEIVGIVNEHGKVEVHRKSCNELYTKIKDNQTKEIQADWFTNSDDDPSFKARLAVTLKNIPGS
IAKITATLAREGVDIRSFDMISVDNKQAKLSCVVVVRNRRELYLLVRLVRKLDVCLNIERILNK

Sequences:

>Translated_704_residues
MFCFYDLNQLISQYLPHRERLKIAQAFIFGADAHETQVRSSGEPYFTHPVAVACILAELRMDVDTIIAALLHDVVEDTEY
TVDDISNIFGKKVAQLVEGVTKLTQIRHKNRAEQQAENFRKMLLSVTKDVRVIFIKLADRLHNMRTLAPLKPEKKRRISK
ETLDVFAPLAHRLGINTLKEQLETLAFEGIYPYRYHILEEKVKKVEKNKEKVFYEVKEALAEKLNDLVSLEDIKARKKTL
YSIYNKMRKKGISFDEIMDMYAYKIIVPNRIDCYVALGKVHELYKPIPQKFKDYIATPKANGYRSLHTVVLGPYNIPLEI
QIKTEQMDRQAEYGIAAHWSYKIGEKTDKALQRWLKKISDINVYTASSVEFLENVKTDIFNNDVFVFTPQGEIVELPMNS
TCIDFAYYIHTDIGNKCISAKVNRKSVPLNYRLKQGDNVEIITSAIADPNPAWLKFVETNRAKSAIKDFLKQQYRNIDYI
RGKDIVEGELRLLGVELRDVPSEIIDGALFNYEGIDTINRFYLDTGLGLIDPNNFIDFVVKHFEDMRNAYKKSSLSKLQI
RYGDDPRIADCCLPLPNDEIVGIVNEHGKVEVHRKSCNELYTKIKDNQTKEIQADWFTNSDDDPSFKARLAVTLKNIPGS
IAKITATLAREGVDIRSFDMISVDNKQAKLSCVVVVRNRRELYLLVRLVRKLDVCLNIERILNK
>Mature_704_residues
MFCFYDLNQLISQYLPHRERLKIAQAFIFGADAHETQVRSSGEPYFTHPVAVACILAELRMDVDTIIAALLHDVVEDTEY
TVDDISNIFGKKVAQLVEGVTKLTQIRHKNRAEQQAENFRKMLLSVTKDVRVIFIKLADRLHNMRTLAPLKPEKKRRISK
ETLDVFAPLAHRLGINTLKEQLETLAFEGIYPYRYHILEEKVKKVEKNKEKVFYEVKEALAEKLNDLVSLEDIKARKKTL
YSIYNKMRKKGISFDEIMDMYAYKIIVPNRIDCYVALGKVHELYKPIPQKFKDYIATPKANGYRSLHTVVLGPYNIPLEI
QIKTEQMDRQAEYGIAAHWSYKIGEKTDKALQRWLKKISDINVYTASSVEFLENVKTDIFNNDVFVFTPQGEIVELPMNS
TCIDFAYYIHTDIGNKCISAKVNRKSVPLNYRLKQGDNVEIITSAIADPNPAWLKFVETNRAKSAIKDFLKQQYRNIDYI
RGKDIVEGELRLLGVELRDVPSEIIDGALFNYEGIDTINRFYLDTGLGLIDPNNFIDFVVKHFEDMRNAYKKSSLSKLQI
RYGDDPRIADCCLPLPNDEIVGIVNEHGKVEVHRKSCNELYTKIKDNQTKEIQADWFTNSDDDPSFKARLAVTLKNIPGS
IAKITATLAREGVDIRSFDMISVDNKQAKLSCVVVVRNRRELYLLVRLVRKLDVCLNIERILNK

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Homo sapiens, GI38348360, Length=141, Percent_Identity=36.1702127659575, Blast_Score=71, Evalue=4e-12,
Organism=Escherichia coli, GI1790082, Length=711, Percent_Identity=41.2095639943741, Blast_Score=563, Evalue=1e-161,
Organism=Escherichia coli, GI1789147, Length=697, Percent_Identity=32.5681492109039, Blast_Score=370, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI25143118, Length=140, Percent_Identity=36.4285714285714, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24650996, Length=135, Percent_Identity=34.8148148148148, Blast_Score=69, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 81140; Mature: 81140

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFCFYDLNQLISQYLPHRERLKIAQAFIFGADAHETQVRSSGEPYFTHPVAVACILAELR
CEEEECHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHCCCCCCEEHHHHHHHHHHHHH
MDVDTIIAALLHDVVEDTEYTVDDISNIFGKKVAQLVEGVTKLTQIRHKNRAEQQAENFR
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KMLLSVTKDVRVIFIKLADRLHNMRTLAPLKPEKKRRISKETLDVFAPLAHRLGINTLKE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHH
QLETLAFEGIYPYRYHILEEKVKKVEKNKEKVFYEVKEALAEKLNDLVSLEDIKARKKTL
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YSIYNKMRKKGISFDEIMDMYAYKIIVPNRIDCYVALGKVHELYKPIPQKFKDYIATPKA
HHHHHHHHHCCCCHHHHHHHHHHEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCC
NGYRSLHTVVLGPYNIPLEIQIKTEQMDRQAEYGIAAHWSYKIGEKTDKALQRWLKKISD
CCCCEEEEEEECCCCCCEEEEEEHHHHHHHHHCCEEEEEEEECCCHHHHHHHHHHHHHHC
INVYTASSVEFLENVKTDIFNNDVFVFTPQGEIVELPMNSTCIDFAYYIHTDIGNKCISA
CCEEECCHHHHHHHHHHHHCCCCEEEECCCCCEEECCCCCCHHHHHHEEECCCCCHHHCC
KVNRKSVPLNYRLKQGDNVEIITSAIADPNPAWLKFVETNRAKSAIKDFLKQQYRNIDYI
CCCCCCCCEEEEECCCCCEEEEEHHHCCCCHHHEEEEHHHHHHHHHHHHHHHHHCCCCCC
RGKDIVEGELRLLGVELRDVPSEIIDGALFNYEGIDTINRFYLDTGLGLIDPNNFIDFVV
CCCCCCCCCCEEEEEHHHHCCHHHHCCHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHH
KHFEDMRNAYKKSSLSKLQIRYGDDPRIADCCLPLPNDEIVGIVNEHGKVEVHRKSCNEL
HHHHHHHHHHHHCCCCEEEEEECCCCCHHHHCCCCCCCCEEEEECCCCEEEEHHHHHHHH
YTKIKDNQTKEIQADWFTNSDDDPSFKARLAVTLKNIPGSIAKITATLAREGVDIRSFDM
HHHHCCCCCCEEEECCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHCCCCCCCEEE
ISVDNKQAKLSCVVVVRNRRELYLLVRLVRKLDVCLNIERILNK
EEECCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFCFYDLNQLISQYLPHRERLKIAQAFIFGADAHETQVRSSGEPYFTHPVAVACILAELR
CEEEECHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHCCCCCCEEHHHHHHHHHHHHH
MDVDTIIAALLHDVVEDTEYTVDDISNIFGKKVAQLVEGVTKLTQIRHKNRAEQQAENFR
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KMLLSVTKDVRVIFIKLADRLHNMRTLAPLKPEKKRRISKETLDVFAPLAHRLGINTLKE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHH
QLETLAFEGIYPYRYHILEEKVKKVEKNKEKVFYEVKEALAEKLNDLVSLEDIKARKKTL
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YSIYNKMRKKGISFDEIMDMYAYKIIVPNRIDCYVALGKVHELYKPIPQKFKDYIATPKA
HHHHHHHHHCCCCHHHHHHHHHHEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCC
NGYRSLHTVVLGPYNIPLEIQIKTEQMDRQAEYGIAAHWSYKIGEKTDKALQRWLKKISD
CCCCEEEEEEECCCCCCEEEEEEHHHHHHHHHCCEEEEEEEECCCHHHHHHHHHHHHHHC
INVYTASSVEFLENVKTDIFNNDVFVFTPQGEIVELPMNSTCIDFAYYIHTDIGNKCISA
CCEEECCHHHHHHHHHHHHCCCCEEEECCCCCEEECCCCCCHHHHHHEEECCCCCHHHCC
KVNRKSVPLNYRLKQGDNVEIITSAIADPNPAWLKFVETNRAKSAIKDFLKQQYRNIDYI
CCCCCCCCEEEEECCCCCEEEEEHHHCCCCHHHEEEEHHHHHHHHHHHHHHHHHCCCCCC
RGKDIVEGELRLLGVELRDVPSEIIDGALFNYEGIDTINRFYLDTGLGLIDPNNFIDFVV
CCCCCCCCCCEEEEEHHHHCCHHHHCCHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHH
KHFEDMRNAYKKSSLSKLQIRYGDDPRIADCCLPLPNDEIVGIVNEHGKVEVHRKSCNEL
HHHHHHHHHHHHCCCCEEEEEECCCCCHHHHCCCCCCCCEEEEECCCCEEEEHHHHHHHH
YTKIKDNQTKEIQADWFTNSDDDPSFKARLAVTLKNIPGSIAKITATLAREGVDIRSFDM
HHHHCCCCCCEEEECCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHCCCCCCCEEE
ISVDNKQAKLSCVVVVRNRRELYLLVRLVRKLDVCLNIERILNK
EEECCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]