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The map label for this gene is iscA [H]

Identifier: 89256636

GI number: 89256636

Start: 1266955

End: 1267311

Strand: Reverse

Name: iscA [H]

Synonym: FTL_1332

Alternate gene names: 89256636

Gene position: 1267311-1266955 (Counterclockwise)

Preceding gene: 89256637

Following gene: 89256635

Centisome position: 66.84

GC content: 33.33

Gene sequence:

>357_bases
ATGGTAGAAGTTTTTGATCCAAATGCTAGTAGCATTTTAGAAGTTACAGATGCTGCAGCTAAACATTTCAAGAAACATCT
AGACAAGCATGATGGTTGTATAGGTATTTATGTCGGAACTAAGGTTATGGGTTGTTCTGGCTTAGCTTATGATGTTGATT
TTGTTAAACAGCAACCACAAGATACACAGAAAGTACAGCAGCATGGCATTAATTTCTTTGTATCTAATAAGTCTATGGAT
TTTCTTAATGGGCTAAAAATTGATTATGTTAAGCATGATTTTGGATTATATAAGTTAGAATATACAAATCCAAACGAATC
AGCACGTTGTGGTTGTGGTGAAAGTTTTACAGTTTAG

Upstream 100 bases:

>100_bases
CTTTTGGGATGTATAATACTTTTGAGGAAATAGATTTGTTCATAATAGCATTGAAAAAAGTAATATCTCAATTAAAATAA
TTTAGCAAAAAGAGGCAAGT

Downstream 100 bases:

>100_bases
GAATATATAAATAATGAAACCAACAGATGTAACAATAATAAGAAGACAGGTAAAAGCAATTGCTGTACCATTTGGTAATG
AGATTGAGTTAATGCCTGGT

Product: hesB family protein

Products: NA

Alternate protein names: Iron-sulfur cluster assembly protein [H]

Number of amino acids: Translated: 118; Mature: 118

Protein sequence:

>118_residues
MVEVFDPNASSILEVTDAAAKHFKKHLDKHDGCIGIYVGTKVMGCSGLAYDVDFVKQQPQDTQKVQQHGINFFVSNKSMD
FLNGLKIDYVKHDFGLYKLEYTNPNESARCGCGESFTV

Sequences:

>Translated_118_residues
MVEVFDPNASSILEVTDAAAKHFKKHLDKHDGCIGIYVGTKVMGCSGLAYDVDFVKQQPQDTQKVQQHGINFFVSNKSMD
FLNGLKIDYVKHDFGLYKLEYTNPNESARCGCGESFTV
>Mature_118_residues
MVEVFDPNASSILEVTDAAAKHFKKHLDKHDGCIGIYVGTKVMGCSGLAYDVDFVKQQPQDTQKVQQHGINFFVSNKSMD
FLNGLKIDYVKHDFGLYKLEYTNPNESARCGCGESFTV

Specific function: Is able to transfer iron-sulfur clusters to apo- ferredoxin. Multiple cycles of [2Fe2S] cluster formation and transfer are observed, suggesting that iscA acts catalytically. Recruits intracellular free iron so as to provide iron for the assembly of transi

COG id: COG0316

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesB/iscA family [H]

Homologues:

Organism=Homo sapiens, GI52426768, Length=108, Percent_Identity=31.4814814814815, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1787974, Length=118, Percent_Identity=34.7457627118644, Blast_Score=76, Evalue=5e-16,
Organism=Escherichia coli, GI1788877, Length=107, Percent_Identity=35.5140186915888, Blast_Score=74, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI193209025, Length=117, Percent_Identity=27.3504273504274, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI25146411, Length=108, Percent_Identity=28.7037037037037, Blast_Score=64, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323001, Length=108, Percent_Identity=28.7037037037037, Blast_Score=64, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24642263, Length=108, Percent_Identity=32.4074074074074, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000361
- InterPro:   IPR016092
- InterPro:   IPR017870
- InterPro:   IPR011302 [H]

Pfam domain/function: PF01521 Fe-S_biosyn [H]

EC number: NA

Molecular weight: Translated: 13171; Mature: 13171

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEVFDPNASSILEVTDAAAKHFKKHLDKHDGCIGIYVGTKVMGCSGLAYDVDFVKQQPQ
CCEEECCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEECCCCCEECHHHHHCCCC
DTQKVQQHGINFFVSNKSMDFLNGLKIDYVKHDFGLYKLEYTNPNESARCGCGESFTV
HHHHHHHCCCEEEECCCCCHHHCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MVEVFDPNASSILEVTDAAAKHFKKHLDKHDGCIGIYVGTKVMGCSGLAYDVDFVKQQPQ
CCEEECCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEECCCCCEECHHHHHCCCC
DTQKVQQHGINFFVSNKSMDFLNGLKIDYVKHDFGLYKLEYTNPNESARCGCGESFTV
HHHHHHHCCCEEEECCCCCHHHCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]