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The map label for this gene is oprF [H]

Identifier: 89256632

GI number: 89256632

Start: 1264352

End: 1265530

Strand: Reverse

Name: oprF [H]

Synonym: FTL_1328

Alternate gene names: 89256632

Gene position: 1265530-1264352 (Counterclockwise)

Preceding gene: 89256633

Following gene: 89256631

Centisome position: 66.75

GC content: 38.0

Gene sequence:

>1179_bases
ATGAGATTAAAAAGTATTGTTATAGCTACAACTGTATTATTAGGTTCAGCTACAGCATCTATCGCTGCAGGTTCAGATAA
TATCGATACGTCAGCAAACACTAATTCAGCTACTACACAAAGCAGTGGTTTTGCAGCTAATAATTTCATTGCTCCTTTTG
CAAATACTTATAGCGCTTTGACTAACAAGGACAATACTTGGGGTCCTCAAGATAGAACTGGCCAGTGGTACTTAGGTGTA
GATGCTAACGGTCTAGCTGGAACTCCTAACTCTCCATCAGGTGCTGGTGCTAACTTCACAATCGGTTATAACATCAATAA
ATACTTCGCTGTACAGTACAACCAATTAGTTGGTAGAGTATTTGCTGGTTTAGGTGAAGGTGTTGTAAACTTTAGTAATA
ATACTATGTTTACTCCATATGCTGCAGGTGGTGCTGGTTGGGCAAATCTAGCAGGTCAAGCAACAGGTGCTTGGGATGTG
GGTGGTGGTCTTAAGTTTGAACTATCTAGAAATGTTCAAGCAAGTGTTGACTACAGATATATCCAAACAATGGCACCTAG
TAATATTTCTGGTGCTAATGGCAGAGCGGGTACTAACATGATTGGTGCTGGTTTAACATGGTTCTTTGGTGGCAAAGATA
CTACTAATAATGACACTGGTAATATTCAGGATAATGGTGCGACTACAGCTGCTCAAACTGTTGCTATGCCAACTATTGAT
GAGTCTAAGTATGTTTTACCTGCTGGTATTAAGCAATGTGAAGGCAACTTTAATCTAACTGAAGATGGTGTCGCGTGCTA
TACAGTAAATGGTGATGAGGTAACAGTTTACCTAGATACTAAGTTTGCTTATGATAAAGCTACTTTAAATGCTAAAGGTA
AAAAAGCTATTGCATCTTTTGTTAATTTTATCAAGGATAGTAACATTAGCTCTGTAACAGTTAAAGGTTATGCTTCTCAA
GGTCAAACTGGTAGTGAGTTTGATATATATAACCAAAAACTTTCTGAGAAGAGAGCACAAGCTGTTGCTGATTACATGAA
GCAATTAGGTTTAGATAGTGAGAAAATAATTACTAAAGGTTTTGGCTATAATGATACTTTAGGTGGTATTCATAAGTCTG
ATCCGCGTAACCAGCGTGTAGAAGCTAGCGTATCAGCTCCACTTAAAGAAGCTAACTAA

Upstream 100 bases:

>100_bases
GCTATAAAAAACTCTTTGATTTTTATTTTAAATCATGTAGAATACTTGAATCTTATGTTTCGGCATGTGAATAGTTAAAA
AGTATTTAAAGGAGTTTTTG

Downstream 100 bases:

>100_bases
TATCAATTTTTTTTAAAATCTCTTAATTTCTTTACGTCATTAATAAATGTTCTAAGCAATAAATTAAAGTATTGTATCTC
TAATTATTCAAATACTTACT

Product: outer membrane associated protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MRLKSIVIATTVLLGSATASIAAGSDNIDTSANTNSATTQSSGFAANNFIAPFANTYSALTNKDNTWGPQDRTGQWYLGV
DANGLAGTPNSPSGAGANFTIGYNINKYFAVQYNQLVGRVFAGLGEGVVNFSNNTMFTPYAAGGAGWANLAGQATGAWDV
GGGLKFELSRNVQASVDYRYIQTMAPSNISGANGRAGTNMIGAGLTWFFGGKDTTNNDTGNIQDNGATTAAQTVAMPTID
ESKYVLPAGIKQCEGNFNLTEDGVACYTVNGDEVTVYLDTKFAYDKATLNAKGKKAIASFVNFIKDSNISSVTVKGYASQ
GQTGSEFDIYNQKLSEKRAQAVADYMKQLGLDSEKIITKGFGYNDTLGGIHKSDPRNQRVEASVSAPLKEAN

Sequences:

>Translated_392_residues
MRLKSIVIATTVLLGSATASIAAGSDNIDTSANTNSATTQSSGFAANNFIAPFANTYSALTNKDNTWGPQDRTGQWYLGV
DANGLAGTPNSPSGAGANFTIGYNINKYFAVQYNQLVGRVFAGLGEGVVNFSNNTMFTPYAAGGAGWANLAGQATGAWDV
GGGLKFELSRNVQASVDYRYIQTMAPSNISGANGRAGTNMIGAGLTWFFGGKDTTNNDTGNIQDNGATTAAQTVAMPTID
ESKYVLPAGIKQCEGNFNLTEDGVACYTVNGDEVTVYLDTKFAYDKATLNAKGKKAIASFVNFIKDSNISSVTVKGYASQ
GQTGSEFDIYNQKLSEKRAQAVADYMKQLGLDSEKIITKGFGYNDTLGGIHKSDPRNQRVEASVSAPLKEAN
>Mature_392_residues
MRLKSIVIATTVLLGSATASIAAGSDNIDTSANTNSATTQSSGFAANNFIAPFANTYSALTNKDNTWGPQDRTGQWYLGV
DANGLAGTPNSPSGAGANFTIGYNINKYFAVQYNQLVGRVFAGLGEGVVNFSNNTMFTPYAAGGAGWANLAGQATGAWDV
GGGLKFELSRNVQASVDYRYIQTMAPSNISGANGRAGTNMIGAGLTWFFGGKDTTNNDTGNIQDNGATTAAQTVAMPTID
ESKYVLPAGIKQCEGNFNLTEDGVACYTVNGDEVTVYLDTKFAYDKATLNAKGKKAIASFVNFIKDSNISSVTVKGYASQ
GQTGSEFDIYNQKLSEKRAQAVADYMKQLGLDSEKIITKGFGYNDTLGGIHKSDPRNQRVEASVSAPLKEAN

Specific function: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium [H]

COG id: COG2885

COG function: function code M; Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

None

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1480 Molecules/Cell In: Growth Phase, Mi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 41260; Mature: 41260

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLKSIVIATTVLLGSATASIAAGSDNIDTSANTNSATTQSSGFAANNFIAPFANTYSAL
CCCHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCCCCCCCCCCCCCCCEECCHHHHHHHH
TNKDNTWGPQDRTGQWYLGVDANGLAGTPNSPSGAGANFTIGYNINKYFAVQYNQLVGRV
CCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEECCCEEEEEEHHHHHHHH
FAGLGEGVVNFSNNTMFTPYAAGGAGWANLAGQATGAWDVGGGLKFELSRNVQASVDYRY
HHHCCCCEEEECCCEEEEEECCCCCCHHHCCCCCCCCEECCCCEEEEECCCCEEEECEEE
IQTMAPSNISGANGRAGTNMIGAGLTWFFGGKDTTNNDTGNIQDNGATTAAQTVAMPTID
EEEECCCCCCCCCCCCCCCEECCCEEEEECCCCCCCCCCCCCCCCCCCHHHHEEECCCCC
ESKYVLPAGIKQCEGNFNLTEDGVACYTVNGDEVTVYLDTKFAYDKATLNAKGKKAIASF
CCCEECCCCHHHCCCCCCCCCCCEEEEEECCCEEEEEEECEEEECCEECCCCCHHHHHHH
VNFIKDSNISSVTVKGYASQGQTGSEFDIYNQKLSEKRAQAVADYMKQLGLDSEKIITKG
HHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEEC
FGYNDTLGGIHKSDPRNQRVEASVSAPLKEAN
CCCCCCCCCCCCCCCCCCEEEEHHCCCCCCCC
>Mature Secondary Structure
MRLKSIVIATTVLLGSATASIAAGSDNIDTSANTNSATTQSSGFAANNFIAPFANTYSAL
CCCHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCCCCCCCCCCCCCCCEECCHHHHHHHH
TNKDNTWGPQDRTGQWYLGVDANGLAGTPNSPSGAGANFTIGYNINKYFAVQYNQLVGRV
CCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEECCCEEEEEEHHHHHHHH
FAGLGEGVVNFSNNTMFTPYAAGGAGWANLAGQATGAWDVGGGLKFELSRNVQASVDYRY
HHHCCCCEEEECCCEEEEEECCCCCCHHHCCCCCCCCEECCCCEEEEECCCCEEEECEEE
IQTMAPSNISGANGRAGTNMIGAGLTWFFGGKDTTNNDTGNIQDNGATTAAQTVAMPTID
EEEECCCCCCCCCCCCCCCEECCCEEEEECCCCCCCCCCCCCCCCCCCHHHHEEECCCCC
ESKYVLPAGIKQCEGNFNLTEDGVACYTVNGDEVTVYLDTKFAYDKATLNAKGKKAIASF
CCCEECCCCHHHCCCCCCCCCCCEEEEEECCCEEEEEEECEEEECCEECCCCCHHHHHHH
VNFIKDSNISSVTVKGYASQGQTGSEFDIYNQKLSEKRAQAVADYMKQLGLDSEKIITKG
HHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEEC
FGYNDTLGGIHKSDPRNQRVEASVSAPLKEAN
CCCCCCCCCCCCCCCCCCEEEEHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2447060; 10984043 [H]