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The map label for this gene is bioD [H]

Identifier: 89256584

GI number: 89256584

Start: 1215645

End: 1216304

Strand: Direct

Name: bioD [H]

Synonym: FTL_1275

Alternate gene names: 89256584

Gene position: 1215645-1216304 (Clockwise)

Preceding gene: 89256583

Following gene: 89256588

Centisome position: 64.12

GC content: 30.3

Gene sequence:

>660_bases
ATGAAAAAGTTTTTTATCATTGGTACGAATACCGAAGTAGGTAAAACATATATATCAACCAAGCTTATTGAAGTTTGTGA
GCATCAGAATATTAAATCACTTTGTTTGAAACCTGTAGCGTCTGGACAAAGTCAATTTTCAGAGCTGTGTGAAGATGTAG
AAAGTATTCTAAATGCTTATAAGCATAAATTTACAGCAGCTGAAATAAATTTAATTTCATTCAATCAAGCAGTTGCTCCT
CATATAATTGCAGCAAAAACAAAAGTAGATATTTCTATTGAGAATCTTAAGCAATTTATCGAAGATAAGTACAATCAAGA
TTTAGATATTCTCTTTATTGAGGGTGCTGGAGGATTATTAACTCCCTACTCAGATCACACTACCCAGCTTGATCTAATAA
AGGCTCTGCAAATACCAGTACTACTAGTATCAGCTATAAAAGTTGGTTGCATAAACCATACCTTACTAACTATAAATGAG
TTAAATCGTCATAATATTAAATTAGCTGGTTGGATTGCTAATTGTAATGACTCAAATATCAAATACATTGATGAACAAAT
AAATACTATAGAAGAGCTATCAGGATATAAGTGTAGTGCAAAAATTTCTCGAAATGCTGATTATTTAGATTTTATAGATT
TATCCAAGATACTAATATAG

Upstream 100 bases:

>100_bases
CAAACAAAATTTCTCGAGTATTAGAAAGCTTTGTTTAAGCTCTAATCATATAAACCTCAGTTACCATGTTGGTCTATTTA
TTTGTTTTAAGGAATAAGTT

Downstream 100 bases:

>100_bases
CCAGAAGAAAATGAATAATTCCCTTCGCTATTCTTAATAAGAATTTCGCCATTATTAGCTAAGTCAACATAGCTACAGTT
ATTATAAGTTTTATCTGCTA

Product: dethiobiotin synthetase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase [H]

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MKKFFIIGTNTEVGKTYISTKLIEVCEHQNIKSLCLKPVASGQSQFSELCEDVESILNAYKHKFTAAEINLISFNQAVAP
HIIAAKTKVDISIENLKQFIEDKYNQDLDILFIEGAGGLLTPYSDHTTQLDLIKALQIPVLLVSAIKVGCINHTLLTINE
LNRHNIKLAGWIANCNDSNIKYIDEQINTIEELSGYKCSAKISRNADYLDFIDLSKILI

Sequences:

>Translated_219_residues
MKKFFIIGTNTEVGKTYISTKLIEVCEHQNIKSLCLKPVASGQSQFSELCEDVESILNAYKHKFTAAEINLISFNQAVAP
HIIAAKTKVDISIENLKQFIEDKYNQDLDILFIEGAGGLLTPYSDHTTQLDLIKALQIPVLLVSAIKVGCINHTLLTINE
LNRHNIKLAGWIANCNDSNIKYIDEQINTIEELSGYKCSAKISRNADYLDFIDLSKILI
>Mature_219_residues
MKKFFIIGTNTEVGKTYISTKLIEVCEHQNIKSLCLKPVASGQSQFSELCEDVESILNAYKHKFTAAEINLISFNQAVAP
HIIAAKTKVDISIENLKQFIEDKYNQDLDILFIEGAGGLLTPYSDHTTQLDLIKALQIPVLLVSAIKVGCINHTLLTINE
LNRHNIKLAGWIANCNDSNIKYIDEQINTIEELSGYKCSAKISRNADYLDFIDLSKILI

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1786995, Length=194, Percent_Identity=32.9896907216495, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI87081944, Length=215, Percent_Identity=27.4418604651163, Blast_Score=81, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004472
- InterPro:   IPR002586 [H]

Pfam domain/function: PF01656 CbiA [H]

EC number: =6.3.3.3 [H]

Molecular weight: Translated: 24503; Mature: 24503

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFFIIGTNTEVGKTYISTKLIEVCEHQNIKSLCLKPVASGQSQFSELCEDVESILNAY
CCEEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
KHKFTAAEINLISFNQAVAPHIIAAKTKVDISIENLKQFIEDKYNQDLDILFIEGAGGLL
HHCCCCEEEEEEECCCCCCCCEEEECEEEEEEHHHHHHHHHHHCCCCEEEEEEECCCCCC
TPYSDHTTQLDLIKALQIPVLLVSAIKVGCINHTLLTINELNRHNIKLAGWIANCNDSNI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEEEEEEECCCCCCC
KYIDEQINTIEELSGYKCSAKISRNADYLDFIDLSKILI
CHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MKKFFIIGTNTEVGKTYISTKLIEVCEHQNIKSLCLKPVASGQSQFSELCEDVESILNAY
CCEEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
KHKFTAAEINLISFNQAVAPHIIAAKTKVDISIENLKQFIEDKYNQDLDILFIEGAGGLL
HHCCCCEEEEEEECCCCCCCCEEEECEEEEEEHHHHHHHHHHHCCCCEEEEEEECCCCCC
TPYSDHTTQLDLIKALQIPVLLVSAIKVGCINHTLLTINELNRHNIKLAGWIANCNDSNI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEEEEEEECCCCCCC
KYIDEQINTIEELSGYKCSAKISRNADYLDFIDLSKILI
CHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA