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The map label for this gene is ptsN [H]

Identifier: 89256501

GI number: 89256501

Start: 1130860

End: 1131315

Strand: Direct

Name: ptsN [H]

Synonym: FTL_1180

Alternate gene names: 89256501

Gene position: 1130860-1131315 (Clockwise)

Preceding gene: 89256500

Following gene: 89256502

Centisome position: 59.64

GC content: 27.63

Gene sequence:

>456_bases
ATGAATTTAAAAGCTCTAATTGATAAAAAAAATATTATCTTAAATTTGAATATTGAGTCTAAAAAACGCTTAATCGAGTT
TTTTGCAAATAGAATAGCAGACACTTACCCTGATGTGAGTGAAGATCTTGCGCTTAAAAATATTTATAAACGCGAAAGAA
TAGGTAATACTTATATTGGTAAAAATATTTACATTCCTCATTGTCGCGTTGAGAATCTTATGACTACTAGGTTGATTATC
GTGACATTAAAAAATAGTTACTATGATGATTCTGTTAACGATGATATAAAGATAGCAGTTGGTGTTTTTTTTCCTGACAA
TATATCTACAATCCATATGGAACTGCTAAAGCAATTAGCTTTATATTTGAAACAAGATAAAACGCAGCAGTATTTTCAGC
AAGCAGAAAATTCCGAAGATTTGTATAATTTAATTATTAATACTAGTAATGAATAA

Upstream 100 bases:

>100_bases
AGATAAGTTAGCACGTCAGTTAAAAAAGCACAAAGATAAAATGAGATGTAATCACGGCGAATAGTAGTCTATCTTATTCA
AATCTATTCAATTTTTACCT

Downstream 100 bases:

>100_bases
TCTATTTAATAATATACGTATAGTTCTAGTAGAGCCATCACACAGTGGTAATGTTGGTTCTACTGCTAGAGCAATGCTTA
ATATGGGGTTGACAAACTTA

Product: PEP-dependent sugar phosphotransferase system (PTS) family protein

Products: protein histidine; sugarphosphate [C]

Alternate protein names: Enzyme IIA-NTR; Phosphotransferase enzyme IIA component; PTS system EIIA component [H]

Number of amino acids: Translated: 151; Mature: 151

Protein sequence:

>151_residues
MNLKALIDKKNIILNLNIESKKRLIEFFANRIADTYPDVSEDLALKNIYKRERIGNTYIGKNIYIPHCRVENLMTTRLII
VTLKNSYYDDSVNDDIKIAVGVFFPDNISTIHMELLKQLALYLKQDKTQQYFQQAENSEDLYNLIINTSNE

Sequences:

>Translated_151_residues
MNLKALIDKKNIILNLNIESKKRLIEFFANRIADTYPDVSEDLALKNIYKRERIGNTYIGKNIYIPHCRVENLMTTRLII
VTLKNSYYDDSVNDDIKIAVGVFFPDNISTIHMELLKQLALYLKQDKTQQYFQQAENSEDLYNLIINTSNE
>Mature_151_residues
MNLKALIDKKNIILNLNIESKKRLIEFFANRIADTYPDVSEDLALKNIYKRERIGNTYIGKNIYIPHCRVENLMTTRLII
VTLKNSYYDDSVNDDIKIAVGVFFPDNISTIHMELLKQLALYLKQDKTQQYFQQAENSEDLYNLIINTSNE

Specific function: Seems to have a role in regulating nitrogen assimilation [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69 [C]

Molecular weight: Translated: 17667; Mature: 17667

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLKALIDKKNIILNLNIESKKRLIEFFANRIADTYPDVSEDLALKNIYKRERIGNTYIG
CCCHHEECCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEECC
KNIYIPHCRVENLMTTRLIIVTLKNSYYDDSVNDDIKIAVGVFFPDNISTIHMELLKQLA
CCEEECCCCHHHHHHEEEEEEEECCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHH
LYLKQDKTQQYFQQAENSEDLYNLIINTSNE
HHHHCCHHHHHHHHCCCCCCCEEEEEECCCC
>Mature Secondary Structure
MNLKALIDKKNIILNLNIESKKRLIEFFANRIADTYPDVSEDLALKNIYKRERIGNTYIG
CCCHHEECCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEECC
KNIYIPHCRVENLMTTRLIIVTLKNSYYDDSVNDDIKIAVGVFFPDNISTIHMELLKQLA
CCEEECCCCHHHHHHEEEEEEEECCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHH
LYLKQDKTQQYFQQAENSEDLYNLIINTSNE
HHHHCCHHHHHHHHCCCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Protein Npi-phosphohistidine; sugar [C]

Specific reaction: Protein Npi-phosphohistidine + sugar = protein histidine + sugarphosphate [C]

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]