Click here to switch to the map view.

The map label for this gene is nth [H]

Identifier: 89256272

GI number: 89256272

Start: 895587

End: 896225

Strand: Reverse

Name: nth [H]

Synonym: FTL_0921

Alternate gene names: 89256272

Gene position: 896225-895587 (Counterclockwise)

Preceding gene: 89256273

Following gene: 89256271

Centisome position: 47.27

GC content: 33.33

Gene sequence:

>639_bases
ATGAATAAGCAAAAAAGAATCCAGATTTTTGAAACATGGAAACAAAATGACCCAAAACCAACAACAGAACTTGAGTATAC
ATCTAATTTTGAACTTCTAATTGCAGTAATTTTATCAGCTCAAGCAACTGACGTAGGTGTCAATAAAGCTACTAAAGTAT
TATTTAAAGTTGCGAATACTCCAGAACAAATATACGCTCTAGGTGAGCAAAAATTAGCTGAATATATAAAATCTATAGGT
CTATACAAAACCAAAGCTAAAAATGTCATAGCTACATGTAAAGATTTGATTGAAAAGTTTGGTAGCATAGTACCAGATAA
TTTTGATGATCTGATATCGCTAGCTGGTGTCGGCAGAAAAACTGCAAATGTAGTGCTTAATACTGCATTTAGTAAACCTA
CCATGGCTGTTGATACCCATATTTTTAGGCTTGCAAATCGTATCCCATTAGCTAAGGGTAAAAACGTCAATGAAGTTGAG
AAAAAGCTTTTACGTGTTATCCCTAAAGAGTATTTACATGATGCACACCACTGGATAATATTACATGGTCGCTATATCTG
TACTGCACAAAGACCAAGGTGTCGTAACTGTATAATTTATGACTACTGTGAATTTAAAGATAAGGAAAAATATCGATGA

Upstream 100 bases:

>100_bases
GTTTACAAAAACATCGCAACTGCTCAAGAAATTGATAAAAAAGCCTATATAGCTGCTTCATTAGCTAAGTTTAGAAATAA
AAAGAAATCTTCACCAACCA

Downstream 100 bases:

>100_bases
TCCTCTCTCAAGATAGATATCAGTTACGCAAACTTTTTATCGATAGCTGGAATAAGTTTGTCAACAACACACCTTTAACT
GCTCTAGAAGAGCAAATAGT

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MNKQKRIQIFETWKQNDPKPTTELEYTSNFELLIAVILSAQATDVGVNKATKVLFKVANTPEQIYALGEQKLAEYIKSIG
LYKTKAKNVIATCKDLIEKFGSIVPDNFDDLISLAGVGRKTANVVLNTAFSKPTMAVDTHIFRLANRIPLAKGKNVNEVE
KKLLRVIPKEYLHDAHHWIILHGRYICTAQRPRCRNCIIYDYCEFKDKEKYR

Sequences:

>Translated_212_residues
MNKQKRIQIFETWKQNDPKPTTELEYTSNFELLIAVILSAQATDVGVNKATKVLFKVANTPEQIYALGEQKLAEYIKSIG
LYKTKAKNVIATCKDLIEKFGSIVPDNFDDLISLAGVGRKTANVVLNTAFSKPTMAVDTHIFRLANRIPLAKGKNVNEVE
KKLLRVIPKEYLHDAHHWIILHGRYICTAQRPRCRNCIIYDYCEFKDKEKYR
>Mature_212_residues
MNKQKRIQIFETWKQNDPKPTTELEYTSNFELLIAVILSAQATDVGVNKATKVLFKVANTPEQIYALGEQKLAEYIKSIG
LYKTKAKNVIATCKDLIEKFGSIVPDNFDDLISLAGVGRKTANVVLNTAFSKPTMAVDTHIFRLANRIPLAKGKNVNEVE
KKLLRVIPKEYLHDAHHWIILHGRYICTAQRPRCRNCIIYDYCEFKDKEKYR

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=27.683615819209, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI1787920, Length=208, Percent_Identity=59.6153846153846, Blast_Score=262, Evalue=1e-71,
Organism=Caenorhabditis elegans, GI17554540, Length=175, Percent_Identity=31.4285714285714, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324530, Length=196, Percent_Identity=27.0408163265306, Blast_Score=70, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6319304, Length=174, Percent_Identity=28.1609195402299, Blast_Score=69, Evalue=5e-13,
Organism=Drosophila melanogaster, GI45550361, Length=192, Percent_Identity=31.7708333333333, Blast_Score=99, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24303; Mature: 24303

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: PS00764 ENDONUCLEASE_III_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKQKRIQIFETWKQNDPKPTTELEYTSNFELLIAVILSAQATDVGVNKATKVLFKVANT
CCCCHHHHHHHHHCCCCCCCCCCEEECCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
PEQIYALGEQKLAEYIKSIGLYKTKAKNVIATCKDLIEKFGSIVPDNFDDLISLAGVGRK
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCH
TANVVLNTAFSKPTMAVDTHIFRLANRIPLAKGKNVNEVEKKLLRVIPKEYLHDAHHWII
HHHHHEECCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHCCCCEEEE
LHGRYICTAQRPRCRNCIIYDYCEFKDKEKYR
EECEEEEECCCCCCCCCEEEECCCCCCCCCCC
>Mature Secondary Structure
MNKQKRIQIFETWKQNDPKPTTELEYTSNFELLIAVILSAQATDVGVNKATKVLFKVANT
CCCCHHHHHHHHHCCCCCCCCCCEEECCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
PEQIYALGEQKLAEYIKSIGLYKTKAKNVIATCKDLIEKFGSIVPDNFDDLISLAGVGRK
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCH
TANVVLNTAFSKPTMAVDTHIFRLANRIPLAKGKNVNEVEKKLLRVIPKEYLHDAHHWII
HHHHHEECCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHCCCCEEEE
LHGRYICTAQRPRCRNCIIYDYCEFKDKEKYR
EECEEEEECCCCCCCCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]