Click here to switch to the map view.

The map label for this gene is hupB [H]

Identifier: 89256252

GI number: 89256252

Start: 874083

End: 874355

Strand: Direct

Name: hupB [H]

Synonym: FTL_0895

Alternate gene names: 89256252

Gene position: 874083-874355 (Clockwise)

Preceding gene: 89256251

Following gene: 89256253

Centisome position: 46.1

GC content: 36.63

Gene sequence:

>273_bases
ATGAACAAGAGTGAATTAGTAAGTGCTATAGCTAAAGAAGCAGATGTTACTAAAGAAGTTGCTAGCAACACTCTAGATGC
TACTATTGCGGCAGTAACTAAAGCTTTAAAAAATGGTGATAGCGTAACTCTAGTAGGTTTTGGTACTTTTCAGGTAAAAG
AAAGAAGTCCTAGAGAGGGTAGAAACCCAAAGACTGGTGAAACTATTAAGATCCCTGCTTCTAAAGTTCCTAGCTTTAAA
GCTAGTAAGGGTCTTAAAGACGCTGTAAAATAA

Upstream 100 bases:

>100_bases
AGAGAGTTTTTTGATATTTAACTGTTGAAACACTGCATTTTTAGTGTTATAAAGTTAACTGACACTTGTCAATTGTATAA
AAATAAAGGAGTTTAAAAAC

Downstream 100 bases:

>100_bases
TAAAAAGTTACAAAAAAGTAATTTTTTTAAAGGCATGCTTAACAGTATGCCTTTTTTTTATCTATAATATTTACAAGTTA
ATTAAATTACTCATTAATTA

Product: histone-like protein HU form B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 90; Mature: 90

Protein sequence:

>90_residues
MNKSELVSAIAKEADVTKEVASNTLDATIAAVTKALKNGDSVTLVGFGTFQVKERSPREGRNPKTGETIKIPASKVPSFK
ASKGLKDAVK

Sequences:

>Translated_90_residues
MNKSELVSAIAKEADVTKEVASNTLDATIAAVTKALKNGDSVTLVGFGTFQVKERSPREGRNPKTGETIKIPASKVPSFK
ASKGLKDAVK
>Mature_90_residues
MNKSELVSAIAKEADVTKEVASNTLDATIAAVTKALKNGDSVTLVGFGTFQVKERSPREGRNPKTGETIKIPASKVPSFK
ASKGLKDAVK

Specific function: Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions [H]

COG id: COG0776

COG function: function code L; Bacterial nucleoid DNA-binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial histone-like protein family [H]

Homologues:

Organism=Escherichia coli, GI1786644, Length=89, Percent_Identity=62.9213483146067, Blast_Score=114, Evalue=1e-27,
Organism=Escherichia coli, GI1790433, Length=90, Percent_Identity=54.4444444444444, Blast_Score=104, Evalue=9e-25,
Organism=Escherichia coli, GI1787141, Length=88, Percent_Identity=32.9545454545455, Blast_Score=67, Evalue=3e-13,
Organism=Escherichia coli, GI1788005, Length=90, Percent_Identity=34.4444444444444, Blast_Score=65, Evalue=8e-13,

Paralogues:

None

Copy number: 860 Molecules/Cell In: Growth-Phase, Minimal Media (Based on E. coli). 2040 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000119
- InterPro:   IPR020816
- InterPro:   IPR010992 [H]

Pfam domain/function: PF00216 Bac_DNA_binding [H]

EC number: NA

Molecular weight: Translated: 9474; Mature: 9474

Theoretical pI: Translated: 10.59; Mature: 10.59

Prosite motif: PS00045 HISTONE_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKSELVSAIAKEADVTKEVASNTLDATIAAVTKALKNGDSVTLVGFGTFQVKERSPREG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCEEEECCCCCCCC
RNPKTGETIKIPASKVPSFKASKGLKDAVK
CCCCCCCEEECCCCCCCCCCCCCCCHHHCC
>Mature Secondary Structure
MNKSELVSAIAKEADVTKEVASNTLDATIAAVTKALKNGDSVTLVGFGTFQVKERSPREG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCEEEECCCCCCCC
RNPKTGETIKIPASKVPSFKASKGLKDAVK
CCCCCCCEEECCCCCCCCCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]