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The map label for this gene is tig [H]

Identifier: 89256248

GI number: 89256248

Start: 868423

End: 869739

Strand: Direct

Name: tig [H]

Synonym: FTL_0891

Alternate gene names: 89256248

Gene position: 868423-869739 (Clockwise)

Preceding gene: 89256247

Following gene: 89256249

Centisome position: 45.8

GC content: 34.24

Gene sequence:

>1317_bases
ATGCAAGTTACTTTAGAGAAAAAAGAAGGAATTCACTGTTCACTATTAATCGAAGTACCAGCTAACGAAATTGATAGTGT
AGTTTCAAAAGAAATCAATAGAACTGCAAAAACTATCAAAATGGATGGTTTTAGACCTGGTAAAGTGCCTGCTGGTATGG
TTAAGAAGAAGTATGGTGAGCAAATCCGTATGGAAGTGATTTCTGATCTTATTCCTCAAAAATACTCTAAAGCTATTCAA
GATGAGAAGCTAGCAGTTGCTGGTATTGAAGTTGAATTAAAAGAAAATAAAGAAGGTCAGCCTCTTAAGTTTGTTGCTAA
TCTTGAGCTTTTTCCTGAGTTTGAGGTAACTGGTTTTGAAAAAATCGAAGTTCAAAAGCCAGTTGTTGAATTAACTGATA
AAGAAGTTAAGCAAATGATCGACAATCTGAGAAAGCAGTTTGCAACTTTCTCAGAAGTTTATAAAGTTGTAGAAAAAGAT
GACAAAGTAACGATAGATTTCGTTGGTAAGAAAGATGGAGAAGCCTTCGAAGGTGGTACAGCAAATGATATAGATGTTAT
CATCGGTTCTGGTCAAATGATTCCAGGCTTTGAAGATGGTATCATCGGTATGAAAAAAGGTGAGCAAAAGACTATTACTG
TAACTTTTCCACAAGACTACCAAAACAAAGATCTTGCCGGGGCTGAGACTACTTTTGATATTACTGTTAAGAAAATTCAA
CAAGCTGAGCTACCAGAGGTTAATGATGAGTTTGTTAAGAAATTTGGTGTAAAAGGCGGTGTCGATACCTTTGAAAACGA
AATCAAAGAAAATATGCAGCGTGAGCTGAAATTTATTCTACAAAGAAAAGTGAAAGACCAAGTTTTCAAAGGTCTAAGGG
AAATAGCTAAGTTTGAAACTCCTAAATCTTTGATTAAGAGAGAGATTGATGCAGCTAAACAAAACCTACTTAAGCAAATG
GGAGGCGCTAAAGGTTTTGATGTTAATCAATTACCTGATAACTTATTTGAAGCTAATGCTAAACAAAAAGTAGAAACTAG
CCTAATACTAGATAGTATTATGAATTCGCAAGAGTTTAAGGCAGAAGAGGCAGAAGTTGAGAGTTTATTAGATGAACTAG
TACAAGCTTATGAAGAGCCAGAAAAAACTAAAGAGCAAATCAAGAAAAATGATAAAGAAATAGCTAACTTAAAAGCGTTA
GTGATAGAAAATAAGCTAACAGATTGGGTATTAGAGCAAGCTAAAGTAACAGAAAAAACTGAAGATTTCTTTGAGGTTAT
TAAAGAAAATATGCAAGCTCAACAGGCTGGCTTCTAA

Upstream 100 bases:

>100_bases
TTGTCATATATTTAATAATAAACATATAGACAATCTAATATATGATAGTTAAAATTTTCTATTAGAAAATTGTTAAATAA
ATAAAAAGGAAAACTTAATA

Downstream 100 bases:

>100_bases
GCTGGCTTTAAAAGAGAGGTTATAAATGATAACTAATAATCTAGTTCCTACCGTAATTGAAAAAACAGCTGGCGGTGAGA
GAGCCTTTGATATTTATTCA

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF [H]

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MQVTLEKKEGIHCSLLIEVPANEIDSVVSKEINRTAKTIKMDGFRPGKVPAGMVKKKYGEQIRMEVISDLIPQKYSKAIQ
DEKLAVAGIEVELKENKEGQPLKFVANLELFPEFEVTGFEKIEVQKPVVELTDKEVKQMIDNLRKQFATFSEVYKVVEKD
DKVTIDFVGKKDGEAFEGGTANDIDVIIGSGQMIPGFEDGIIGMKKGEQKTITVTFPQDYQNKDLAGAETTFDITVKKIQ
QAELPEVNDEFVKKFGVKGGVDTFENEIKENMQRELKFILQRKVKDQVFKGLREIAKFETPKSLIKREIDAAKQNLLKQM
GGAKGFDVNQLPDNLFEANAKQKVETSLILDSIMNSQEFKAEEAEVESLLDELVQAYEEPEKTKEQIKKNDKEIANLKAL
VIENKLTDWVLEQAKVTEKTEDFFEVIKENMQAQQAGF

Sequences:

>Translated_438_residues
MQVTLEKKEGIHCSLLIEVPANEIDSVVSKEINRTAKTIKMDGFRPGKVPAGMVKKKYGEQIRMEVISDLIPQKYSKAIQ
DEKLAVAGIEVELKENKEGQPLKFVANLELFPEFEVTGFEKIEVQKPVVELTDKEVKQMIDNLRKQFATFSEVYKVVEKD
DKVTIDFVGKKDGEAFEGGTANDIDVIIGSGQMIPGFEDGIIGMKKGEQKTITVTFPQDYQNKDLAGAETTFDITVKKIQ
QAELPEVNDEFVKKFGVKGGVDTFENEIKENMQRELKFILQRKVKDQVFKGLREIAKFETPKSLIKREIDAAKQNLLKQM
GGAKGFDVNQLPDNLFEANAKQKVETSLILDSIMNSQEFKAEEAEVESLLDELVQAYEEPEKTKEQIKKNDKEIANLKAL
VIENKLTDWVLEQAKVTEKTEDFFEVIKENMQAQQAGF
>Mature_438_residues
MQVTLEKKEGIHCSLLIEVPANEIDSVVSKEINRTAKTIKMDGFRPGKVPAGMVKKKYGEQIRMEVISDLIPQKYSKAIQ
DEKLAVAGIEVELKENKEGQPLKFVANLELFPEFEVTGFEKIEVQKPVVELTDKEVKQMIDNLRKQFATFSEVYKVVEKD
DKVTIDFVGKKDGEAFEGGTANDIDVIIGSGQMIPGFEDGIIGMKKGEQKTITVTFPQDYQNKDLAGAETTFDITVKKIQ
QAELPEVNDEFVKKFGVKGGVDTFENEIKENMQRELKFILQRKVKDQVFKGLREIAKFETPKSLIKREIDAAKQNLLKQM
GGAKGFDVNQLPDNLFEANAKQKVETSLILDSIMNSQEFKAEEAEVESLLDELVQAYEEPEKTKEQIKKNDKEIANLKAL
VIENKLTDWVLEQAKVTEKTEDFFEVIKENMQAQQAGF

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation [H]

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain [H]

Homologues:

Organism=Escherichia coli, GI1786640, Length=435, Percent_Identity=40.9195402298851, Blast_Score=328, Evalue=3e-91,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881 [H]

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N [H]

EC number: NA

Molecular weight: Translated: 49571; Mature: 49571

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVTLEKKEGIHCSLLIEVPANEIDSVVSKEINRTAKTIKMDGFRPGKVPAGMVKKKYGE
CCEEEECCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHH
QIRMEVISDLIPQKYSKAIQDEKLAVAGIEVELKENKEGQPLKFVANLELFPEFEVTGFE
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCEEEEECCEECCCCCCCCCH
KIEVQKPVVELTDKEVKQMIDNLRKQFATFSEVYKVVEKDDKVTIDFVGKKDGEAFEGGT
HEEHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCC
ANDIDVIIGSGQMIPGFEDGIIGMKKGEQKTITVTFPQDYQNKDLAGAETTFDITVKKIQ
CCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHH
QAELPEVNDEFVKKFGVKGGVDTFENEIKENMQRELKFILQRKVKDQVFKGLREIAKFET
HCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PKSLIKREIDAAKQNLLKQMGGAKGFDVNQLPDNLFEANAKQKVETSLILDSIMNSQEFK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCHHHHHCCHHHHHHHHHHHHHHHCCHHHH
AEEAEVESLLDELVQAYEEPEKTKEQIKKNDKEIANLKALVIENKLTDWVLEQAKVTEKT
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EDFFEVIKENMQAQQAGF
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQVTLEKKEGIHCSLLIEVPANEIDSVVSKEINRTAKTIKMDGFRPGKVPAGMVKKKYGE
CCEEEECCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHH
QIRMEVISDLIPQKYSKAIQDEKLAVAGIEVELKENKEGQPLKFVANLELFPEFEVTGFE
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCEEEEECCEECCCCCCCCCH
KIEVQKPVVELTDKEVKQMIDNLRKQFATFSEVYKVVEKDDKVTIDFVGKKDGEAFEGGT
HEEHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCC
ANDIDVIIGSGQMIPGFEDGIIGMKKGEQKTITVTFPQDYQNKDLAGAETTFDITVKKIQ
CCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHH
QAELPEVNDEFVKKFGVKGGVDTFENEIKENMQRELKFILQRKVKDQVFKGLREIAKFET
HCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PKSLIKREIDAAKQNLLKQMGGAKGFDVNQLPDNLFEANAKQKVETSLILDSIMNSQEFK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCHHHHHCCHHHHHHHHHHHHHHHCCHHHH
AEEAEVESLLDELVQAYEEPEKTKEQIKKNDKEIANLKALVIENKLTDWVLEQAKVTEKT
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EDFFEVIKENMQAQQAGF
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA