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The map label for this gene is ispF [H]

Identifier: 89256193

GI number: 89256193

Start: 816566

End: 817045

Strand: Reverse

Name: ispF [H]

Synonym: FTL_0833

Alternate gene names: 89256193

Gene position: 817045-816566 (Counterclockwise)

Preceding gene: 89256194

Following gene: 89256190

Centisome position: 43.09

GC content: 31.67

Gene sequence:

>480_bases
ATGTCATTTCGTATAGGTCATGGTTATGATGTGCATAAGTTCACTTCAGCAAAGCAAAATATCATTATTGGTGGAGTCGA
AATTGCTTATCATCTAGGACTTGAAGCTCATTCTGATGGTGATGTACTAATACATGCTCTTTGTGATGCAATTCTTGGTG
CTTTAGGACTAGGTGATATAGGCAAACATTTCCTGGATACGGATAATCAATTTAAAAATATTGATAGTAAATTTTTCTTA
GCTGAAATAAAAAAAATGCTTGATGAAAAACAATATTCCATAAGCAATATTGATTGTACTATAATTGCTCAAGCTCCTAA
GATGCTTCCACATATTGAAAAAATGAGAGCTTGTCTAGCAAATATTCTTGAAATACAAATTAGCCAGATAAATATCAAAG
CTACTACAACTGAAAGATTAGGATTTATTGGTAGAGAAGAAGGTATTGCAACCCATGTGGTTTGTTTATTATATAGATAA

Upstream 100 bases:

>100_bases
ACAGCATATGGGTTATACAAACGTAGTTTCTATAGCTGGTGGAATCAAGGATTGGCTAGCTAATAACTATCCAGTTTCTC
AAAATTAAGGATAAAGAATA

Downstream 100 bases:

>100_bases
ATCGCTATTTTTTTGCACACTCTTCAAGATAGTTTGCTACTTCTTCAAGTTCAGCTTGACAACAGAATATTGCCATATCG
TTACTTTCTAGCTTTTCAAC

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 159; Mature: 158

Protein sequence:

>159_residues
MSFRIGHGYDVHKFTSAKQNIIIGGVEIAYHLGLEAHSDGDVLIHALCDAILGALGLGDIGKHFLDTDNQFKNIDSKFFL
AEIKKMLDEKQYSISNIDCTIIAQAPKMLPHIEKMRACLANILEIQISQINIKATTTERLGFIGREEGIATHVVCLLYR

Sequences:

>Translated_159_residues
MSFRIGHGYDVHKFTSAKQNIIIGGVEIAYHLGLEAHSDGDVLIHALCDAILGALGLGDIGKHFLDTDNQFKNIDSKFFL
AEIKKMLDEKQYSISNIDCTIIAQAPKMLPHIEKMRACLANILEIQISQINIKATTTERLGFIGREEGIATHVVCLLYR
>Mature_158_residues
SFRIGHGYDVHKFTSAKQNIIIGGVEIAYHLGLEAHSDGDVLIHALCDAILGALGLGDIGKHFLDTDNQFKNIDSKFFLA
EIKKMLDEKQYSISNIDCTIIAQAPKMLPHIEKMRACLANILEIQISQINIKATTTERLGFIGREEGIATHVVCLLYR

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP [H]

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family [H]

Homologues:

Organism=Escherichia coli, GI1789103, Length=157, Percent_Identity=56.0509554140127, Blast_Score=165, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF02542 YgbB [H]

EC number: =4.6.1.12 [H]

Molecular weight: Translated: 17666; Mature: 17535

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFRIGHGYDVHKFTSAKQNIIIGGVEIAYHLGLEAHSDGDVLIHALCDAILGALGLGDI
CCEECCCCCCHHHHCCCCCCEEEEHHEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCHH
GKHFLDTDNQFKNIDSKFFLAEIKKMLDEKQYSISNIDCTIIAQAPKMLPHIEKMRACLA
HHHHCCCCCHHHCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHH
NILEIQISQINIKATTTERLGFIGREEGIATHVVCLLYR
HHHHHHHEEEEEEEECHHHHCCCCCCCCHHEEHEEEECC
>Mature Secondary Structure 
SFRIGHGYDVHKFTSAKQNIIIGGVEIAYHLGLEAHSDGDVLIHALCDAILGALGLGDI
CEECCCCCCHHHHCCCCCCEEEEHHEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCHH
GKHFLDTDNQFKNIDSKFFLAEIKKMLDEKQYSISNIDCTIIAQAPKMLPHIEKMRACLA
HHHHCCCCCHHHCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHH
NILEIQISQINIKATTTERLGFIGREEGIATHVVCLLYR
HHHHHHHEEEEEEEECHHHHCCCCCCCCHHEEHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA