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The map label for this gene is pilC [H]

Identifier: 89256187

GI number: 89256187

Start: 807237

End: 808466

Strand: Reverse

Name: pilC [H]

Synonym: FTL_0827

Alternate gene names: 89256187

Gene position: 808466-807237 (Counterclockwise)

Preceding gene: 89256188

Following gene: 89256184

Centisome position: 42.64

GC content: 34.31

Gene sequence:

>1230_bases
ATGCTATTTGGCAAAAAAGATAAAAATAAAAGAACTATTACATCATGGAATTATAAAGCTAAGCTAAAAAGTGGTAAAAA
GACTAAAGGTAGTATTGATGCTGTCACCAAAGAGAAGGCAGAAATTCAATTAAGACAAAAGGGCTACTCTGATATCAAAG
TCAAAAAACAACCTAAAGATCTATTACCTAAGAAGATATCTTATCAAGATATTACCGAGATGACGCGCCAGCTAGCAATA
ATGACCAAAGCTGGTATTCCCATAATCAAATCATTTGAAGTATTTATTATGGGTATTCGTAAACATCCGCGCCTTAAAAT
ATTAGCTATCGAGATAAAGCAAGCTATCGAAGGGGGCGAGTCATTTTCAAAGGCTCTCAGAAACTTTCCTAAGATATTTG
ATAAATTATATGTTGGTCTTATTACTGCTGGTGAAGAATCAGGGAATCTTGATCTAATGCTTAATAAAATTGCTGATCAG
CGTGAAGCACTACAGAGTATCAAAAAGAAAGTAAAAAAAGCACTATCTTACCCAATTATAGTATGTATTATCGCTGCTGG
GGTTACAGGTATCCTATTAACATTTGCTGTGCCAGCTTTCTCAGCAATGTTTATAAATTCAGGAAAACCACTACCAGCAA
TCACACAATTAACTGTTGATGCATCTAACTTTATGCAAAATTCATGGTGGAAAATTATTCTGGCAATATTTATCACAATA
ACAGTTTATAAATCATTGAAATTTAGATTTCCTAAAATCCAAATTGCCCAAGATCATTTTATGTTAAAAGTACCAATAAT
TGGTGAAGTTGTATTTAAATCATCACTAGCAAGATTTGCTAGTACTTTAGAGATAACCGTCCAATCTGGTATGAGTCTGA
CACGTGCTCTAGATATGGTGTCTTTAGCAACGGGAAATGCAAAGTATGATCAAGCTGCTTTAGATATTAAAAAGAGTATT
AATGAAGGTGCCTCATTTAAAGAAGCAATCGTTGAAACTGGAAACTTCCCGTTTTTAGTTGAACAAATGATTGCTGTAGG
TGAGGAATCAGGTGCTCTAGAGACAATGCTAGGCAACCTAAATAGAGTATACCAAGAAGAAGTAGATACATTAGTAGGAA
GCTTAAGCTCAATGCTTGAACCTTTGATTATGATTGTCTTAGGTGGTGTTGTTGGTTTTCTCGTAGTTAGTATGTATATG
CCGATGTTTCAAATGGGTGATGCGATATAA

Upstream 100 bases:

>100_bases
AAGGAATCGCTACTGTCAGACAATCAGCTCTAGTCAGGGTTGCTGAAGATCTAACATCAATGGAAGAAGTATACCGTGTA
AGTTAAAGGGAATAAAAAAT

Downstream 100 bases:

>100_bases
TATACTTTTAAAAATTAGCGTCGAATTTATCCTTTTATATTCAAGATGAATATTTTTTATACTAGAATTTCAATTATTTA
ATATCTGGCCAGTACCAATT

Product: Type IV pili polytopic inner membrane protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MLFGKKDKNKRTITSWNYKAKLKSGKKTKGSIDAVTKEKAEIQLRQKGYSDIKVKKQPKDLLPKKISYQDITEMTRQLAI
MTKAGIPIIKSFEVFIMGIRKHPRLKILAIEIKQAIEGGESFSKALRNFPKIFDKLYVGLITAGEESGNLDLMLNKIADQ
REALQSIKKKVKKALSYPIIVCIIAAGVTGILLTFAVPAFSAMFINSGKPLPAITQLTVDASNFMQNSWWKIILAIFITI
TVYKSLKFRFPKIQIAQDHFMLKVPIIGEVVFKSSLARFASTLEITVQSGMSLTRALDMVSLATGNAKYDQAALDIKKSI
NEGASFKEAIVETGNFPFLVEQMIAVGEESGALETMLGNLNRVYQEEVDTLVGSLSSMLEPLIMIVLGGVVGFLVVSMYM
PMFQMGDAI

Sequences:

>Translated_409_residues
MLFGKKDKNKRTITSWNYKAKLKSGKKTKGSIDAVTKEKAEIQLRQKGYSDIKVKKQPKDLLPKKISYQDITEMTRQLAI
MTKAGIPIIKSFEVFIMGIRKHPRLKILAIEIKQAIEGGESFSKALRNFPKIFDKLYVGLITAGEESGNLDLMLNKIADQ
REALQSIKKKVKKALSYPIIVCIIAAGVTGILLTFAVPAFSAMFINSGKPLPAITQLTVDASNFMQNSWWKIILAIFITI
TVYKSLKFRFPKIQIAQDHFMLKVPIIGEVVFKSSLARFASTLEITVQSGMSLTRALDMVSLATGNAKYDQAALDIKKSI
NEGASFKEAIVETGNFPFLVEQMIAVGEESGALETMLGNLNRVYQEEVDTLVGSLSSMLEPLIMIVLGGVVGFLVVSMYM
PMFQMGDAI
>Mature_409_residues
MLFGKKDKNKRTITSWNYKAKLKSGKKTKGSIDAVTKEKAEIQLRQKGYSDIKVKKQPKDLLPKKISYQDITEMTRQLAI
MTKAGIPIIKSFEVFIMGIRKHPRLKILAIEIKQAIEGGESFSKALRNFPKIFDKLYVGLITAGEESGNLDLMLNKIADQ
REALQSIKKKVKKALSYPIIVCIIAAGVTGILLTFAVPAFSAMFINSGKPLPAITQLTVDASNFMQNSWWKIILAIFITI
TVYKSLKFRFPKIQIAQDHFMLKVPIIGEVVFKSSLARFASTLEITVQSGMSLTRALDMVSLATGNAKYDQAALDIKKSI
NEGASFKEAIVETGNFPFLVEQMIAVGEESGALETMLGNLNRVYQEEVDTLVGSLSSMLEPLIMIVLGGVVGFLVVSMYM
PMFQMGDAI

Specific function: Involved in the translocation of the type IV pilin (pilA) [H]

COG id: COG1459

COG function: function code NU; Type II secretory pathway, component PulF

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP F family [H]

Homologues:

Organism=Escherichia coli, GI1789724, Length=409, Percent_Identity=29.3398533007335, Blast_Score=178, Evalue=6e-46,
Organism=Escherichia coli, GI1786295, Length=396, Percent_Identity=28.5353535353535, Blast_Score=166, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003004
- InterPro:   IPR018076
- InterPro:   IPR001992 [H]

Pfam domain/function: PF00482 GSPII_F [H]

EC number: NA

Molecular weight: Translated: 45325; Mature: 45325

Theoretical pI: Translated: 10.29; Mature: 10.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFGKKDKNKRTITSWNYKAKLKSGKKTKGSIDAVTKEKAEIQLRQKGYSDIKVKKQPKD
CCCCCCCCCCCCEECCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEECCCCHH
LLPKKISYQDITEMTRQLAIMTKAGIPIIKSFEVFIMGIRKHPRLKILAIEIKQAIEGGE
HCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHEECCCCCCEEEEHHHHHHHHCCCH
SFSKALRNFPKIFDKLYVGLITAGEESGNLDLMLNKIADQREALQSIKKKVKKALSYPII
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
VCIIAAGVTGILLTFAVPAFSAMFINSGKPLPAITQLTVDASNFMQNSWWKIILAIFITI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHEEEECHHHHHHHHHHHHHHHHHHHH
TVYKSLKFRFPKIQIAQDHFMLKVPIIGEVVFKSSLARFASTLEITVQSGMSLTRALDMV
HHHHHHHHCCCEEEEECCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
SLATGNAKYDQAALDIKKSINEGASFKEAIVETGNFPFLVEQMIAVGEESGALETMLGNL
HHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHH
NRVYQEEVDTLVGSLSSMLEPLIMIVLGGVVGFLVVSMYMPMFQMGDAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLFGKKDKNKRTITSWNYKAKLKSGKKTKGSIDAVTKEKAEIQLRQKGYSDIKVKKQPKD
CCCCCCCCCCCCEECCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEECCCCHH
LLPKKISYQDITEMTRQLAIMTKAGIPIIKSFEVFIMGIRKHPRLKILAIEIKQAIEGGE
HCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHEECCCCCCEEEEHHHHHHHHCCCH
SFSKALRNFPKIFDKLYVGLITAGEESGNLDLMLNKIADQREALQSIKKKVKKALSYPII
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
VCIIAAGVTGILLTFAVPAFSAMFINSGKPLPAITQLTVDASNFMQNSWWKIILAIFITI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHEEEECHHHHHHHHHHHHHHHHHHHH
TVYKSLKFRFPKIQIAQDHFMLKVPIIGEVVFKSSLARFASTLEITVQSGMSLTRALDMV
HHHHHHHHCCCEEEEECCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
SLATGNAKYDQAALDIKKSINEGASFKEAIVETGNFPFLVEQMIAVGEESGALETMLGNL
HHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHH
NRVYQEEVDTLVGSLSSMLEPLIMIVLGGVVGFLVVSMYMPMFQMGDAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1971619; 10984043 [H]