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The map label for this gene is smpB [H]

Identifier: 89256144

GI number: 89256144

Start: 751615

End: 752088

Strand: Reverse

Name: smpB [H]

Synonym: FTL_0761

Alternate gene names: 89256144

Gene position: 752088-751615 (Counterclockwise)

Preceding gene: 89256150

Following gene: 89256143

Centisome position: 39.67

GC content: 36.08

Gene sequence:

>474_bases
ATGAGTAAACATAAGGTTTCTCCAGCGACTATTGCTAAAAATAAAAAAGCCTTGCATGATTATACTATTTTAGAGAAATT
TGAAGCCGGAATAGTTCTACAAGGTTGGGAAGTAAAAAGTATTCGCGCTGGTAAAGTACAAATGGTTGATAGTCATGTGC
ATATTAAACATGGTGAAGCGTGGCTTTTTAATTGTCTAATCACACCTTTGCTATCTGCCTCAACTCATGTGGTTGCAGAT
GCTGCAGCAACACGCAAGCTACTTCTCAATCGTCGTGAGATTAATAAGATTATGGGTAGAATTGAGCAAAAAGGTTTTAC
ATGTATACCTCTTTCTATGTATTGGAAAGGTCCACGAGTAAAAGTAGAAATAGCATTAGCTCAAGGTAAAAAAGTTCATG
ATAAGCGCCAAGCACAAAAAGACAAAGACTGGGCACGCGAAAAAGATAGATTATTTAAAAAGGCTTATAAATAA

Upstream 100 bases:

>100_bases
ACTGCAGATTTATTAACCTTATTCATTTATTTAATCTTTTGCGTTTTGCTGATAATATATAGCCTTACATTGTATATCTT
TTACAAAAAATAATAAAGTT

Downstream 100 bases:

>100_bases
ATGACAAAGACCGAGAAAAAACTACGCCACTATATCACCAAGGCTATTGCAGACTATAAGCTTCTTGATAAAGGTGATAA
AGCTATGCTATGCCTATCTG

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 157; Mature: 156

Protein sequence:

>157_residues
MSKHKVSPATIAKNKKALHDYTILEKFEAGIVLQGWEVKSIRAGKVQMVDSHVHIKHGEAWLFNCLITPLLSASTHVVAD
AAATRKLLLNRREINKIMGRIEQKGFTCIPLSMYWKGPRVKVEIALAQGKKVHDKRQAQKDKDWAREKDRLFKKAYK

Sequences:

>Translated_157_residues
MSKHKVSPATIAKNKKALHDYTILEKFEAGIVLQGWEVKSIRAGKVQMVDSHVHIKHGEAWLFNCLITPLLSASTHVVAD
AAATRKLLLNRREINKIMGRIEQKGFTCIPLSMYWKGPRVKVEIALAQGKKVHDKRQAQKDKDWAREKDRLFKKAYK
>Mature_156_residues
SKHKVSPATIAKNKKALHDYTILEKFEAGIVLQGWEVKSIRAGKVQMVDSHVHIKHGEAWLFNCLITPLLSASTHVVADA
AATRKLLLNRREINKIMGRIEQKGFTCIPLSMYWKGPRVKVEIALAQGKKVHDKRQAQKDKDWAREKDRLFKKAYK

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes [H]

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family [H]

Homologues:

Organism=Escherichia coli, GI1788973, Length=154, Percent_Identity=53.2467532467532, Blast_Score=172, Evalue=8e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000037
- InterPro:   IPR020081
- ProDom:   PD004488 [H]

Pfam domain/function: PF01668 SmpB [H]

EC number: NA

Molecular weight: Translated: 17954; Mature: 17823

Theoretical pI: Translated: 10.79; Mature: 10.79

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKHKVSPATIAKNKKALHDYTILEKFEAGIVLQGWEVKSIRAGKVQMVDSHVHIKHGEA
CCCCCCCCHHHHCCCHHHHHHHHHHHHCCCEEEECCEECCCCCCEEEEEECEEEEECCCH
WLFNCLITPLLSASTHVVADAAATRKLLLNRREINKIMGRIEQKGFTCIPLSMYWKGPRV
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHEECCCCE
KVEIALAQGKKVHDKRQAQKDKDWAREKDRLFKKAYK
EEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKHKVSPATIAKNKKALHDYTILEKFEAGIVLQGWEVKSIRAGKVQMVDSHVHIKHGEA
CCCCCCCHHHHCCCHHHHHHHHHHHHCCCEEEECCEECCCCCCEEEEEECEEEEECCCH
WLFNCLITPLLSASTHVVADAAATRKLLLNRREINKIMGRIEQKGFTCIPLSMYWKGPRV
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHEECCCCE
KVEIALAQGKKVHDKRQAQKDKDWAREKDRLFKKAYK
EEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA