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The map label for this gene is rfbB [H]

Identifier: 89256004

GI number: 89256004

Start: 594587

End: 595603

Strand: Direct

Name: rfbB [H]

Synonym: FTL_0606

Alternate gene names: 89256004

Gene position: 594587-595603 (Clockwise)

Preceding gene: 89256003

Following gene: 89256005

Centisome position: 31.36

GC content: 33.43

Gene sequence:

>1017_bases
ATGAGGAATGAAAAAATGAACTACAAACCAAAAAATATCCTAGTAACAGGTGCGGCGGGATTTATTGGTAGTAACTATGT
GCGTATTATGTTATCACGCTATAGTGATATCAAAATAATCTCGTATGATAAGCTTACTTATGCGGGTAGTTTAGATAATC
TAAAAGACTTGAATAATGAACATAACCATATTTTTATAAAAGGTGATATTTGTGATGAAGTTTTAGTATATCAAACACTG
AAAGAATATAAAATTGATACGATAGTACATTTTGCTGCAGAATCGCATGTTGATAATTCAATTGCTAATCCAAAGGTGTT
TTTAGAAACGAATGTGATAGGTACATTTACACTTTTAGATTGTGCTAAAAGGTATTGGTTAGATGAGCTAGGTTTAGAAG
AAACTAGTTGTAGGTTTCATCATATATCTACTGATGAGGTATATGGTACCTTGGCAAAAGATGAACCAGCCTTTACTGAG
ATTAAGGCTTATGAGCCAAATTCACCGTATTCGGCATCTAAGGCGGGATCTGATCATATTGCTAGAGCATATCATCATAC
CTATAAACTTCCGGTAACAATTTCAAATTGTTCAAACAACTATGGACCATACCAACATCGAGAGAAATTAATCCCTGTAG
TGATAAATAGTTGTATAAACTACAAGCCTATTCCTATTTACGGAGATGGTTCGAATATTCGAGATTGGCTATATGTAGAA
GATCACTGCGATGCTATCCAGACAATTGTTGAGAAAGGAGTGGTTGGAGAGGTTTATAATATTGGTGGTATTAATGAAGT
TGATAATCTAACCTTGGTAAAAACTATCTGTAAACTAATGGATGAATATAAACCAGAAAATGCTCCACATTCTAACTTAA
TCACATTTGTGGAAGATAGAAAAGGACATGATTGGCGTTATGCTATTGATAACAGCAAGATTCAGAATGAGTTAGGATGG
AAGCCATCACAAGATTTTGATAAGATGTTTAGACAAACTATTGAGTTTTATCTATAG

Upstream 100 bases:

>100_bases
ATGTGATTGGGAGATTACCGCCTACGCGGTAATGACAAGTTTATGCGGTAATGATAGTTTAGTGAGAGAATGACTAGTCA
CATATAGGAATGATGATGTA

Downstream 100 bases:

>100_bases
CTTAAATATTTATCTTATGAGTATCTCTAAAAAATCAATTTAATTTATTTTTGTGTTAAAAAGTAGTGTTTGCAAGAATA
TAGTTAATCCGAAAGATATT

Product: dTDP-D-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MRNEKMNYKPKNILVTGAAGFIGSNYVRIMLSRYSDIKIISYDKLTYAGSLDNLKDLNNEHNHIFIKGDICDEVLVYQTL
KEYKIDTIVHFAAESHVDNSIANPKVFLETNVIGTFTLLDCAKRYWLDELGLEETSCRFHHISTDEVYGTLAKDEPAFTE
IKAYEPNSPYSASKAGSDHIARAYHHTYKLPVTISNCSNNYGPYQHREKLIPVVINSCINYKPIPIYGDGSNIRDWLYVE
DHCDAIQTIVEKGVVGEVYNIGGINEVDNLTLVKTICKLMDEYKPENAPHSNLITFVEDRKGHDWRYAIDNSKIQNELGW
KPSQDFDKMFRQTIEFYL

Sequences:

>Translated_338_residues
MRNEKMNYKPKNILVTGAAGFIGSNYVRIMLSRYSDIKIISYDKLTYAGSLDNLKDLNNEHNHIFIKGDICDEVLVYQTL
KEYKIDTIVHFAAESHVDNSIANPKVFLETNVIGTFTLLDCAKRYWLDELGLEETSCRFHHISTDEVYGTLAKDEPAFTE
IKAYEPNSPYSASKAGSDHIARAYHHTYKLPVTISNCSNNYGPYQHREKLIPVVINSCINYKPIPIYGDGSNIRDWLYVE
DHCDAIQTIVEKGVVGEVYNIGGINEVDNLTLVKTICKLMDEYKPENAPHSNLITFVEDRKGHDWRYAIDNSKIQNELGW
KPSQDFDKMFRQTIEFYL
>Mature_338_residues
MRNEKMNYKPKNILVTGAAGFIGSNYVRIMLSRYSDIKIISYDKLTYAGSLDNLKDLNNEHNHIFIKGDICDEVLVYQTL
KEYKIDTIVHFAAESHVDNSIANPKVFLETNVIGTFTLLDCAKRYWLDELGLEETSCRFHHISTDEVYGTLAKDEPAFTE
IKAYEPNSPYSASKAGSDHIARAYHHTYKLPVTISNCSNNYGPYQHREKLIPVVINSCINYKPIPIYGDGSNIRDWLYVE
DHCDAIQTIVEKGVVGEVYNIGGINEVDNLTLVKTICKLMDEYKPENAPHSNLITFVEDRKGHDWRYAIDNSKIQNELGW
KPSQDFDKMFRQTIEFYL

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=327, Percent_Identity=37.9204892966361, Blast_Score=216, Evalue=2e-56,
Organism=Homo sapiens, GI42516563, Length=332, Percent_Identity=23.1927710843373, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI4504031, Length=354, Percent_Identity=24.5762711864407, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI48994969, Length=333, Percent_Identity=51.6516516516517, Blast_Score=348, Evalue=2e-97,
Organism=Escherichia coli, GI1788353, Length=334, Percent_Identity=50.5988023952096, Blast_Score=348, Evalue=4e-97,
Organism=Escherichia coli, GI1786974, Length=347, Percent_Identity=26.2247838616715, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1788366, Length=240, Percent_Identity=25.4166666666667, Blast_Score=62, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17568069, Length=336, Percent_Identity=36.0119047619048, Blast_Score=215, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI115532424, Length=332, Percent_Identity=31.3253012048193, Blast_Score=171, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI17539532, Length=251, Percent_Identity=27.0916334661355, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17507723, Length=251, Percent_Identity=25.4980079681275, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319493, Length=262, Percent_Identity=26.3358778625954, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI21356223, Length=338, Percent_Identity=24.2603550295858, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI19923002, Length=362, Percent_Identity=26.2430939226519, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 38787; Mature: 38787

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNEKMNYKPKNILVTGAAGFIGSNYVRIMLSRYSDIKIISYDKLTYAGSLDNLKDLNNE
CCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCEEEEEECCEEECCCCHHHHHCCCC
HNHIFIKGDICDEVLVYQTLKEYKIDTIVHFAAESHVDNSIANPKVFLETNVIGTFTLLD
CCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHH
CAKRYWLDELGLEETSCRFHHISTDEVYGTLAKDEPAFTEIKAYEPNSPYSASKAGSDHI
HHHHHHHHHCCCCCCCCEEEECCHHHHHEECCCCCCCCEEEEEECCCCCCCCCCCCCHHH
ARAYHHTYKLPVTISNCSNNYGPYQHREKLIPVVINSCINYKPIPIYGDGSNIRDWLYVE
HHHHHHEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEC
DHCDAIQTIVEKGVVGEVYNIGGINEVDNLTLVKTICKLMDEYKPENAPHSNLITFVEDR
HHHHHHHHHHHCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECC
KGHDWRYAIDNSKIQNELGWKPSQDFDKMFRQTIEFYL
CCCCEEEEECCCHHHHHCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRNEKMNYKPKNILVTGAAGFIGSNYVRIMLSRYSDIKIISYDKLTYAGSLDNLKDLNNE
CCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCEEEEEECCEEECCCCHHHHHCCCC
HNHIFIKGDICDEVLVYQTLKEYKIDTIVHFAAESHVDNSIANPKVFLETNVIGTFTLLD
CCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHH
CAKRYWLDELGLEETSCRFHHISTDEVYGTLAKDEPAFTEIKAYEPNSPYSASKAGSDHI
HHHHHHHHHCCCCCCCCEEEECCHHHHHEECCCCCCCCEEEEEECCCCCCCCCCCCCHHH
ARAYHHTYKLPVTISNCSNNYGPYQHREKLIPVVINSCINYKPIPIYGDGSNIRDWLYVE
HHHHHHEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEC
DHCDAIQTIVEKGVVGEVYNIGGINEVDNLTLVKTICKLMDEYKPENAPHSNLITFVEDR
HHHHHHHHHHHCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECC
KGHDWRYAIDNSKIQNELGWKPSQDFDKMFRQTIEFYL
CCCCEEEEECCCHHHHHCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253667 [H]