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The map label for this gene is fumB [H]

Identifier: 89255932

GI number: 89255932

Start: 505163

End: 506677

Strand: Direct

Name: fumB [H]

Synonym: FTL_0525

Alternate gene names: 89255932

Gene position: 505163-506677 (Clockwise)

Preceding gene: 89255931

Following gene: 89255933

Centisome position: 26.64

GC content: 38.35

Gene sequence:

>1515_bases
ATGGCTGTTATCAAAGCAGAAGATCTAGTAAATAGTGTTGCAGAAGCATTGCAATATATATCTTATTATCATCCAAAAGA
TTTTATTGATGCAATGTATGAAGCATATCAGCGTGAAGAGTCAAAACCAGCTAAAGATGCAATGGTGCAAATTCTAATAA
ACTCGAGAATGTCTGCTATTGGTAAGCGTCCGATTTGCCAAGATACAGGGATGGTATGCGCTTTTGTCAAAGTTGGTATG
GATGCAAAACTTGACAAGACTGATAGAACAATTACAGAACTTATTAATGAGGGTGTGCGTCGTGGCTATAATGATCCGTA
TAACCCTTTGAGAGCATCTATGGTTTTTCCGCCACATGGATCTAGAAAAAACACTAAAGATAATACTCCAGCAATTGTTC
ATATTGATTTAGTTCATGGTGATAAAATTGAGGTTGATATTGCTGCAAAAGGTGGTGGATCTGAATTTAAATCAAAATTT
AAGGTGTTAAATCCTAGTGATAGCATAATTGATTGGGTTGAAGAGATGTTGCCAACTATGGGGGTTGGCTGGTGTCCACC
TGGAATTATTGGTATAGGTATTGGAGGCACTGCAGAAAAAGCGATGCTTTTGGCTAAGGAATCTCTTAGTGAAGAGATTA
ATATCCAAGATATTATCAAAAATGGCCCACAAAATGAAACAGAGCAACTAAGATTAGATATCTATAATCGTGTCAATGCT
CTGGGTATAGGTGCACAAGGCTTAGGTGGTTTGACAACAGTTTTAGATGTCAAAATAAATGAATATCCAACCCATGCTGC
GTGTAAGCCAGTTGCTTTGATTCCTAACTGTGCAGCTACTCGCCATGTTCATTTTGTTTTAGATGGTTCTGGTGCCGTTG
ATTTACCAGCTCCTAAGATAGAAGACTGGCCAGTAATTGAGCAAGCACAAAATGATGATGTTAAGAGAATAAATCTTGAT
ACAGTTACTAGAGAAGAGATTGAGGCATTAAGGTCGGGAGATAACGTTTTGATTAGCGGTAAAATCTTAACTGGTCGAGA
TGCTGCACATAAGCGTCTACAAGATATGTATAATGCTGGAGAAGATTTTCCTGTTGATCTTAACGGTAGATTTATCTACT
ACGTAGGTCCTGTTGATCCAGTAGGTGATGAGGTGGTTGGTCCAGCTGGTCCAACTACTGCTACGCGTATGGATAAGTTT
ACGCCATTTATGTTAGAGAAAGCTGGTATCATGGGAATGATAGGTAAATCAGAGCGTGGTCAGGCAACGATTGATTCTAT
CAAGAAAAATAAAGCTATTTACTTCATGGGTGTAGGTGGTGCTGCTTATCTCATGTCTAAATCTATCAAAAAAGCAGAAG
TTGTTGCATTTGAAGATCTTGGTATGGAGGCAATTTATGAGTTTGAGGTTGAGGATATGCCAGTAACTGTGGCTGTTGAC
TCTTTAGGTGTTTCTGCTCATCAGCAAGGTCCTAAACTTTGGAAACAAAAAATAGCTGAAATTAATAAGGTCTAA

Upstream 100 bases:

>100_bases
TGATAACTTATACTAAAAGATAAACTAGCTTAAAAATTATACTATTACTGTTATAATAGACAATGAGTATAAGTTTTTTA
AAATAAAGAGGATTAAATAA

Downstream 100 bases:

>100_bases
TCTATGCAAGAGTTAGTATTTTTTATAATTAAGATTTTCTTTGTAATTTTAGCTATTTCTGTAGTTGGCATAGTTAGTGC
TGTAGTTATAAAAAGCTTCA

Product: fumerate hydratase

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 504; Mature: 503

Protein sequence:

>504_residues
MAVIKAEDLVNSVAEALQYISYYHPKDFIDAMYEAYQREESKPAKDAMVQILINSRMSAIGKRPICQDTGMVCAFVKVGM
DAKLDKTDRTITELINEGVRRGYNDPYNPLRASMVFPPHGSRKNTKDNTPAIVHIDLVHGDKIEVDIAAKGGGSEFKSKF
KVLNPSDSIIDWVEEMLPTMGVGWCPPGIIGIGIGGTAEKAMLLAKESLSEEINIQDIIKNGPQNETEQLRLDIYNRVNA
LGIGAQGLGGLTTVLDVKINEYPTHAACKPVALIPNCAATRHVHFVLDGSGAVDLPAPKIEDWPVIEQAQNDDVKRINLD
TVTREEIEALRSGDNVLISGKILTGRDAAHKRLQDMYNAGEDFPVDLNGRFIYYVGPVDPVGDEVVGPAGPTTATRMDKF
TPFMLEKAGIMGMIGKSERGQATIDSIKKNKAIYFMGVGGAAYLMSKSIKKAEVVAFEDLGMEAIYEFEVEDMPVTVAVD
SLGVSAHQQGPKLWKQKIAEINKV

Sequences:

>Translated_504_residues
MAVIKAEDLVNSVAEALQYISYYHPKDFIDAMYEAYQREESKPAKDAMVQILINSRMSAIGKRPICQDTGMVCAFVKVGM
DAKLDKTDRTITELINEGVRRGYNDPYNPLRASMVFPPHGSRKNTKDNTPAIVHIDLVHGDKIEVDIAAKGGGSEFKSKF
KVLNPSDSIIDWVEEMLPTMGVGWCPPGIIGIGIGGTAEKAMLLAKESLSEEINIQDIIKNGPQNETEQLRLDIYNRVNA
LGIGAQGLGGLTTVLDVKINEYPTHAACKPVALIPNCAATRHVHFVLDGSGAVDLPAPKIEDWPVIEQAQNDDVKRINLD
TVTREEIEALRSGDNVLISGKILTGRDAAHKRLQDMYNAGEDFPVDLNGRFIYYVGPVDPVGDEVVGPAGPTTATRMDKF
TPFMLEKAGIMGMIGKSERGQATIDSIKKNKAIYFMGVGGAAYLMSKSIKKAEVVAFEDLGMEAIYEFEVEDMPVTVAVD
SLGVSAHQQGPKLWKQKIAEINKV
>Mature_503_residues
AVIKAEDLVNSVAEALQYISYYHPKDFIDAMYEAYQREESKPAKDAMVQILINSRMSAIGKRPICQDTGMVCAFVKVGMD
AKLDKTDRTITELINEGVRRGYNDPYNPLRASMVFPPHGSRKNTKDNTPAIVHIDLVHGDKIEVDIAAKGGGSEFKSKFK
VLNPSDSIIDWVEEMLPTMGVGWCPPGIIGIGIGGTAEKAMLLAKESLSEEINIQDIIKNGPQNETEQLRLDIYNRVNAL
GIGAQGLGGLTTVLDVKINEYPTHAACKPVALIPNCAATRHVHFVLDGSGAVDLPAPKIEDWPVIEQAQNDDVKRINLDT
VTREEIEALRSGDNVLISGKILTGRDAAHKRLQDMYNAGEDFPVDLNGRFIYYVGPVDPVGDEVVGPAGPTTATRMDKFT
PFMLEKAGIMGMIGKSERGQATIDSIKKNKAIYFMGVGGAAYLMSKSIKKAEVVAFEDLGMEAIYEFEVEDMPVTVAVDS
LGVSAHQQGPKLWKQKIAEINKV

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1790564, Length=472, Percent_Identity=30.9322033898305, Blast_Score=189, Evalue=4e-49,
Organism=Escherichia coli, GI1787897, Length=472, Percent_Identity=30.7203389830508, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI1789442, Length=279, Percent_Identity=28.673835125448, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1789443, Length=189, Percent_Identity=30.6878306878307, Blast_Score=93, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 55065; Mature: 54934

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVIKAEDLVNSVAEALQYISYYHPKDFIDAMYEAYQREESKPAKDAMVQILINSRMSAI
CCEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
GKRPICQDTGMVCAFVKVGMDAKLDKTDRTITELINEGVRRGYNDPYNPLRASMVFPPHG
CCCCCCCCCCCEEEHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCC
SRKNTKDNTPAIVHIDLVHGDKIEVDIAAKGGGSEFKSKFKVLNPSDSIIDWVEEMLPTM
CCCCCCCCCCEEEEEEEECCCEEEEEEEECCCCHHHHHHEEEECCCHHHHHHHHHHHHHC
GVGWCPPGIIGIGIGGTAEKAMLLAKESLSEEINIQDIIKNGPQNETEQLRLDIYNRVNA
CCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHCCCE
LGIGAQGLGGLTTVLDVKINEYPTHAACKPVALIPNCAATRHVHFVLDGSGAVDLPAPKI
EECCCCCCCCEEEEEEEEECCCCCCCCCCCEEECCCCCCCCEEEEEEECCCCCCCCCCCC
EDWPVIEQAQNDDVKRINLDTVTREEIEALRSGDNVLISGKILTGRDAAHKRLQDMYNAG
CCCCCHHCCCCCCCEEEECCHHHHHHHHHHHCCCCEEEEEEEEECCHHHHHHHHHHHHCC
EDFPVDLNGRFIYYVGPVDPVGDEVVGPAGPTTATRMDKFTPFMLEKAGIMGMIGKSERG
CCCEEECCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHCCCEEECCCCCCC
QATIDSIKKNKAIYFMGVGGAAYLMSKSIKKAEVVAFEDLGMEAIYEFEVEDMPVTVAVD
CHHHHHHHCCCEEEEEECCHHHHHHHHCCCHHHEEEEHHCCCEEEEEEEECCCCEEEEEE
SLGVSAHQQGPKLWKQKIAEINKV
CCCCCHHHCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
AVIKAEDLVNSVAEALQYISYYHPKDFIDAMYEAYQREESKPAKDAMVQILINSRMSAI
CEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
GKRPICQDTGMVCAFVKVGMDAKLDKTDRTITELINEGVRRGYNDPYNPLRASMVFPPHG
CCCCCCCCCCCEEEHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCC
SRKNTKDNTPAIVHIDLVHGDKIEVDIAAKGGGSEFKSKFKVLNPSDSIIDWVEEMLPTM
CCCCCCCCCCEEEEEEEECCCEEEEEEEECCCCHHHHHHEEEECCCHHHHHHHHHHHHHC
GVGWCPPGIIGIGIGGTAEKAMLLAKESLSEEINIQDIIKNGPQNETEQLRLDIYNRVNA
CCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHCCCE
LGIGAQGLGGLTTVLDVKINEYPTHAACKPVALIPNCAATRHVHFVLDGSGAVDLPAPKI
EECCCCCCCCEEEEEEEEECCCCCCCCCCCEEECCCCCCCCEEEEEEECCCCCCCCCCCC
EDWPVIEQAQNDDVKRINLDTVTREEIEALRSGDNVLISGKILTGRDAAHKRLQDMYNAG
CCCCCHHCCCCCCCEEEECCHHHHHHHHHHHCCCCEEEEEEEEECCHHHHHHHHHHHHCC
EDFPVDLNGRFIYYVGPVDPVGDEVVGPAGPTTATRMDKFTPFMLEKAGIMGMIGKSERG
CCCEEECCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHCCCEEECCCCCCC
QATIDSIKKNKAIYFMGVGGAAYLMSKSIKKAEVVAFEDLGMEAIYEFEVEDMPVTVAVD
CHHHHHHHCCCEEEEEECCHHHHHHHHCCCHHHEEEEHHCCCEEEEEEEECCCCEEEEEE
SLGVSAHQQGPKLWKQKIAEINKV
CCCCCHHHCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]