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The map label for this gene is metG [H]

Identifier: 89255854

GI number: 89255854

Start: 412077

End: 414101

Strand: Reverse

Name: metG [H]

Synonym: FTL_0444

Alternate gene names: 89255854

Gene position: 414101-412077 (Counterclockwise)

Preceding gene: 89255855

Following gene: 89255853

Centisome position: 21.84

GC content: 34.07

Gene sequence:

>2025_bases
ATGCGAAAGATACTCGTTACTAATGCTCTGCCATATGCTAATGGTGACTTACATTTAGGTCACATGCTCGGCTATATACA
ATCCGACATCTGGGTCAGATTCCAAAAACTACAAGGCAATCAATGCATATTTGTCTGTGGTAGTGATACTCATGGTACGC
CAATAATGCTCAAAGCAAAGAGTCTTGGAATTACTCCAGAAGAGTTAGTTACAAAATACTCTAATAGACACTTACAAGAT
TTTACTGATTTTGAAATTAATTTTGACAATTATCACTCTACTCATAATTCTCTAAATAAAGAGATTGTTGAAGATATATA
TAATAAACTTAACAATAAAAATCTTATTTCAAAAAAAGCAATAGCTCAAGCTTATGACCCTGAGGCTAAAATGTTTTTGC
CAGATAGATTTGTCAAGGGAACTTGTCCAAAATGTAAAGCTGAAGATCAGTATGGTGATAGCTGTGAAGTTTGTGGTGCC
ACATATGATCCTACAGAGCTTATCAATCCAAGATCTGTAATATCTGGTCAATCGCCAATACAAAAAAACTCTGAACATTT
CTTTTTTGACTTACCCGCATTAGAAAAAAATATAAAAGATTGGATAGAATCTAATACACTCTTACAACCAGAAGTTGCTA
ACAAACTAGCAGAATGGTTTGAGCAAGGCTTACAAAGTTGGGATATCTCGCGTGATGCGCCATATTTTGGCTTTGCAATC
CCAGGTACAAATGAACAAAAATTCTTCTATGTGTGGCTAGATGCTCCAATGGGCTATATTGCAAGCTTCAAAGATTATTG
TAATAAAAATAATATTAATTTTGGTGATTTCTGGGGAGATAGTTCTAGTGAAAGTGAGCTTTATCATTTTATTGGTAAAG
ATATTATTTATTTTCATGCACTATTTTGGCCAGCAATATTATCATCTACTGGTTATAAGACACCTACTAGTGTATTTGCT
AATGGTTTCTTAACTGTAAATGGTAAGAAGATGTCAAAATCTCGAGGGACGTTCATTCAAGCTAGAACTTACCTAGATAA
TTTAGAGCCTAGTTATTTAAGATACTACTTTGCGTCAAGATTGACATCCCGCATTGATGATATAGATTTGAATCTAGAGG
AATTTGTTACTAAATCAAATTCTGATATAGTAGGTAAGGTAGTCAATATTGCCAGTCGTTGTGCTGGATTTATATACAAA
AAATTTGATGCTACCTTGTCTGGAGAGATTTTTGATCCAGAATTAGAAAGTGAATTTAGTAAAAACCACGATGCTATAAC
ACAAGCTTTTGAGAAAAGAGAGTTTGCCCACGCTGTGAGACTGATAATGGCATTAGCCGATAAAGCTAATCAATTTATAG
ATTACCATAAACCTTGGCAACTAGCTAAAGAAGAAGGACAAGAACAAAAAGTTCATCAAGTCTGTTCACAAGGTATAAAT
ATGTTTAAGGTTTTAATCGCTTATCTTAAGCCTATTATTCCTAGCATTGTTGCTGAGACAGAGAGATTTTTAAATATACA
GTTCATAAGCTGGGCTGATGCTCCAAAGTTTTTAATAAATCATAAAATAGATAAATTCAAACCTCTAGCCACTCGTATCG
AAAAAGAAAAAGTAGACAAAATTTTAGAGGATACAAAGAAAATGTTTGAAAATGAACAAGCTCCGCAATCTAAAAAAGAA
GAGCCTAAACTAGATATCGCAGCTGAATGTACTTTTGATGATTTTATGAAAGTAGATTTGCGTATAGCTAAAATTACTGA
AGCTTCACATGTTGAAGGTGCGGATAAATTACTTAAACTAATATTGGATCTAGGTGGTGTCACCAAACAAGTTTTTGCTG
GTATCAAGTCTGCTTACAAACCTGAAGATTTAATTGGCAAGCATACTATAATGGTAGCAAATCTAGCTCCTAGAAAAATG
AAATTTGGAATGTCTGAGGGCATGGTTTTAGCTGCTGGAGATGGTAAAGGTATATATATACTTGAGCCACATGAGGGTGC
TCAACCAGGTATGCGTGTCAAATAA

Upstream 100 bases:

>100_bases
CAATTTTAGCAGTATTAATGCACAATTTAGCTAAACAAAATAGTTAAAAAACTTTATAATAACAGAATCATTGTTAATAA
ATTGATATTATAATCAAAAA

Downstream 100 bases:

>100_bases
TATAAGCTCTCCCACAATGAAAAAATATTACTACAATACTTTAATAATTCTTTTACTGTTCTTTTTGGCATTACTAAATT
ATGTTGACAGATCAACTCTA

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 674; Mature: 674

Protein sequence:

>674_residues
MRKILVTNALPYANGDLHLGHMLGYIQSDIWVRFQKLQGNQCIFVCGSDTHGTPIMLKAKSLGITPEELVTKYSNRHLQD
FTDFEINFDNYHSTHNSLNKEIVEDIYNKLNNKNLISKKAIAQAYDPEAKMFLPDRFVKGTCPKCKAEDQYGDSCEVCGA
TYDPTELINPRSVISGQSPIQKNSEHFFFDLPALEKNIKDWIESNTLLQPEVANKLAEWFEQGLQSWDISRDAPYFGFAI
PGTNEQKFFYVWLDAPMGYIASFKDYCNKNNINFGDFWGDSSSESELYHFIGKDIIYFHALFWPAILSSTGYKTPTSVFA
NGFLTVNGKKMSKSRGTFIQARTYLDNLEPSYLRYYFASRLTSRIDDIDLNLEEFVTKSNSDIVGKVVNIASRCAGFIYK
KFDATLSGEIFDPELESEFSKNHDAITQAFEKREFAHAVRLIMALADKANQFIDYHKPWQLAKEEGQEQKVHQVCSQGIN
MFKVLIAYLKPIIPSIVAETERFLNIQFISWADAPKFLINHKIDKFKPLATRIEKEKVDKILEDTKKMFENEQAPQSKKE
EPKLDIAAECTFDDFMKVDLRIAKITEASHVEGADKLLKLILDLGGVTKQVFAGIKSAYKPEDLIGKHTIMVANLAPRKM
KFGMSEGMVLAAGDGKGIYILEPHEGAQPGMRVK

Sequences:

>Translated_674_residues
MRKILVTNALPYANGDLHLGHMLGYIQSDIWVRFQKLQGNQCIFVCGSDTHGTPIMLKAKSLGITPEELVTKYSNRHLQD
FTDFEINFDNYHSTHNSLNKEIVEDIYNKLNNKNLISKKAIAQAYDPEAKMFLPDRFVKGTCPKCKAEDQYGDSCEVCGA
TYDPTELINPRSVISGQSPIQKNSEHFFFDLPALEKNIKDWIESNTLLQPEVANKLAEWFEQGLQSWDISRDAPYFGFAI
PGTNEQKFFYVWLDAPMGYIASFKDYCNKNNINFGDFWGDSSSESELYHFIGKDIIYFHALFWPAILSSTGYKTPTSVFA
NGFLTVNGKKMSKSRGTFIQARTYLDNLEPSYLRYYFASRLTSRIDDIDLNLEEFVTKSNSDIVGKVVNIASRCAGFIYK
KFDATLSGEIFDPELESEFSKNHDAITQAFEKREFAHAVRLIMALADKANQFIDYHKPWQLAKEEGQEQKVHQVCSQGIN
MFKVLIAYLKPIIPSIVAETERFLNIQFISWADAPKFLINHKIDKFKPLATRIEKEKVDKILEDTKKMFENEQAPQSKKE
EPKLDIAAECTFDDFMKVDLRIAKITEASHVEGADKLLKLILDLGGVTKQVFAGIKSAYKPEDLIGKHTIMVANLAPRKM
KFGMSEGMVLAAGDGKGIYILEPHEGAQPGMRVK
>Mature_674_residues
MRKILVTNALPYANGDLHLGHMLGYIQSDIWVRFQKLQGNQCIFVCGSDTHGTPIMLKAKSLGITPEELVTKYSNRHLQD
FTDFEINFDNYHSTHNSLNKEIVEDIYNKLNNKNLISKKAIAQAYDPEAKMFLPDRFVKGTCPKCKAEDQYGDSCEVCGA
TYDPTELINPRSVISGQSPIQKNSEHFFFDLPALEKNIKDWIESNTLLQPEVANKLAEWFEQGLQSWDISRDAPYFGFAI
PGTNEQKFFYVWLDAPMGYIASFKDYCNKNNINFGDFWGDSSSESELYHFIGKDIIYFHALFWPAILSSTGYKTPTSVFA
NGFLTVNGKKMSKSRGTFIQARTYLDNLEPSYLRYYFASRLTSRIDDIDLNLEEFVTKSNSDIVGKVVNIASRCAGFIYK
KFDATLSGEIFDPELESEFSKNHDAITQAFEKREFAHAVRLIMALADKANQFIDYHKPWQLAKEEGQEQKVHQVCSQGIN
MFKVLIAYLKPIIPSIVAETERFLNIQFISWADAPKFLINHKIDKFKPLATRIEKEKVDKILEDTKKMFENEQAPQSKKE
EPKLDIAAECTFDDFMKVDLRIAKITEASHVEGADKLLKLILDLGGVTKQVFAGIKSAYKPEDLIGKHTIMVANLAPRKM
KFGMSEGMVLAAGDGKGIYILEPHEGAQPGMRVK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI14043022, Length=575, Percent_Identity=27.8260869565217, Blast_Score=252, Evalue=9e-67,
Organism=Homo sapiens, GI24308436, Length=517, Percent_Identity=24.5647969052224, Blast_Score=156, Evalue=7e-38,
Organism=Escherichia coli, GI1788432, Length=679, Percent_Identity=55.3755522827688, Blast_Score=807, Evalue=0.0,
Organism=Escherichia coli, GI1789455, Length=104, Percent_Identity=34.6153846153846, Blast_Score=63, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17540794, Length=512, Percent_Identity=30.078125, Blast_Score=228, Evalue=6e-60,
Organism=Caenorhabditis elegans, GI212645096, Length=515, Percent_Identity=23.8834951456311, Blast_Score=131, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI71992781, Length=343, Percent_Identity=24.198250728863, Blast_Score=87, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6321704, Length=564, Percent_Identity=30.8510638297872, Blast_Score=259, Evalue=1e-69,
Organism=Saccharomyces cerevisiae, GI6321610, Length=536, Percent_Identity=23.3208955223881, Blast_Score=110, Evalue=8e-25,
Organism=Drosophila melanogaster, GI24655311, Length=559, Percent_Identity=28.4436493738819, Blast_Score=250, Evalue=3e-66,
Organism=Drosophila melanogaster, GI21358483, Length=581, Percent_Identity=23.2358003442341, Blast_Score=131, Evalue=1e-30,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 76591; Mature: 76591

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: PS50886 TRBD ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKILVTNALPYANGDLHLGHMLGYIQSDIWVRFQKLQGNQCIFVCGSDTHGTPIMLKAK
CCEEEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEEEC
SLGITPEELVTKYSNRHLQDFTDFEINFDNYHSTHNSLNKEIVEDIYNKLNNKNLISKKA
CCCCCHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
IAQAYDPEAKMFLPDRFVKGTCPKCKAEDQYGDSCEVCGATYDPTELINPRSVISGQSPI
HHHHCCCCCCEECCCHHHCCCCCCCCCCCCCCCCHHCCCCCCCHHHHCCCHHHHCCCCCC
QKNSEHFFFDLPALEKNIKDWIESNTLLQPEVANKLAEWFEQGLQSWDISRDAPYFGFAI
CCCCCEEEEECHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEC
PGTNEQKFFYVWLDAPMGYIASFKDYCNKNNINFGDFWGDSSSESELYHFIGKDIIYFHA
CCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LFWPAILSSTGYKTPTSVFANGFLTVNGKKMSKSRGTFIQARTYLDNLEPSYLRYYFASR
HHHHHHHHCCCCCCCHHHHHCCEEEECCCCCCCCCCCEEEEHHHHHCCCHHHHHHHHHHH
LTSRIDDIDLNLEEFVTKSNSDIVGKVVNIASRCAGFIYKKFDATLSGEIFDPELESEFS
HHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
KNHDAITQAFEKREFAHAVRLIMALADKANQFIDYHKPWQLAKEEGQEQKVHQVCSQGIN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHH
MFKVLIAYLKPIIPSIVAETERFLNIQFISWADAPKFLINHKIDKFKPLATRIEKEKVDK
HHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHCCHHHHHHHHHHHHHHHHHH
ILEDTKKMFENEQAPQSKKEEPKLDIAAECTFDDFMKVDLRIAKITEASHVEGADKLLKL
HHHHHHHHHCCCCCCCCCCCCCCCCEEEECCHHHHHHHHEEEEEECCCHHCCCHHHHHHH
ILDLGGVTKQVFAGIKSAYKPEDLIGKHTIMVANLAPRKMKFGMSEGMVLAAGDGKGIYI
HHHHCCHHHHHHHHHHHCCCCHHHCCCCEEEEECCCCHHHHCCCCCCEEEEECCCCEEEE
LEPHEGAQPGMRVK
ECCCCCCCCCCCCC
>Mature Secondary Structure
MRKILVTNALPYANGDLHLGHMLGYIQSDIWVRFQKLQGNQCIFVCGSDTHGTPIMLKAK
CCEEEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEEEC
SLGITPEELVTKYSNRHLQDFTDFEINFDNYHSTHNSLNKEIVEDIYNKLNNKNLISKKA
CCCCCHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
IAQAYDPEAKMFLPDRFVKGTCPKCKAEDQYGDSCEVCGATYDPTELINPRSVISGQSPI
HHHHCCCCCCEECCCHHHCCCCCCCCCCCCCCCCHHCCCCCCCHHHHCCCHHHHCCCCCC
QKNSEHFFFDLPALEKNIKDWIESNTLLQPEVANKLAEWFEQGLQSWDISRDAPYFGFAI
CCCCCEEEEECHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEC
PGTNEQKFFYVWLDAPMGYIASFKDYCNKNNINFGDFWGDSSSESELYHFIGKDIIYFHA
CCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LFWPAILSSTGYKTPTSVFANGFLTVNGKKMSKSRGTFIQARTYLDNLEPSYLRYYFASR
HHHHHHHHCCCCCCCHHHHHCCEEEECCCCCCCCCCCEEEEHHHHHCCCHHHHHHHHHHH
LTSRIDDIDLNLEEFVTKSNSDIVGKVVNIASRCAGFIYKKFDATLSGEIFDPELESEFS
HHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
KNHDAITQAFEKREFAHAVRLIMALADKANQFIDYHKPWQLAKEEGQEQKVHQVCSQGIN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHH
MFKVLIAYLKPIIPSIVAETERFLNIQFISWADAPKFLINHKIDKFKPLATRIEKEKVDK
HHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHCCHHHHHHHHHHHHHHHHHH
ILEDTKKMFENEQAPQSKKEEPKLDIAAECTFDDFMKVDLRIAKITEASHVEGADKLLKL
HHHHHHHHHCCCCCCCCCCCCCCCCEEEECCHHHHHHHHEEEEEECCCHHCCCHHHHHHH
ILDLGGVTKQVFAGIKSAYKPEDLIGKHTIMVANLAPRKMKFGMSEGMVLAAGDGKGIYI
HHHHCCHHHHHHHHHHHCCCCHHHCCCCEEEEECCCCHHHHCCCCCCEEEEECCCCEEEE
LEPHEGAQPGMRVK
ECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA