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The map label for this gene is trxB [C]

Identifier: 89054549

GI number: 89054549

Start: 2066146

End: 2067756

Strand: Direct

Name: trxB [C]

Synonym: Jann_2058

Alternate gene names: 89054549

Gene position: 2066146-2067756 (Clockwise)

Preceding gene: 89054548

Following gene: 89054552

Centisome position: 47.85

GC content: 61.14

Gene sequence:

>1611_bases
ATGCGTGTGGAAAGTCTTGCCGGAAATCTGAGAGAGATGTCGCGCACGCCCCTGCATGACAGCCACGTCGAGGAGTTGCG
CCAGCAAGGCGACGTCGTGCGCTTTTCCAAGGGCCATATCATCGCCGAGGTGGGCGACCCGATGGACGCGTTCATCTACA
TCCTTGAAGGTGGCGTTGAGGTGATTGACCCCTACACCCGCGAAGCCTACATGCCGGCCTCGCTGGGGCCGACCCAGTTC
ATGGGCGAAATCGCATTCCTGAACGGCGGTGCCTATTCGCTGCCGATGCGCGCGAAGGAGGATACGACCGCCATCGTTGT
CCCGCGCCAGAAGATGCTCGATGCGATGGCGCGGATCCCTGAGATGTCGGACATTGTCATCACCGTATTTGCAGCCCGCA
GGCGGCGGCAGATTGAAGACGGGGACAGTTCCCTGAAACTTGTGGGGATGGACATCAGCCGCGATATCCGGGCCGTCGCT
GATTTCGCGTCCCGCAACAAGATCCCGTTCCAAATTCTGGAACCCGGATCGGTTGATGTGCAGGAGACGTCTGAGACCTG
TGGCATTTCCCTCACTGAGCCTGCGGTGATCTTCGGGCATAACCGGGTGGTCGAAGATCCGACCCCGGCCAAGGTGGCGC
AACTTCTCGGGCTTGATCTGGAAATCGCGGAAGATGAGGTTGTGGATGTCCTGATCGTGGGCGGTGGTCCGGCCGGTGTG
GCCGCTGCGGTTTATGCGGGGGCGGAGGGGCTGTCGGCGATTGTGGTGGAGGATGTTGCCGTTGGCGGGCAGGCGGGCAC
TTCGAGCCGGATCGAGAACTACCTGGGGTTTCCCACCGGCATTTCCGGGGCAGATCTTGTGTGGCGTGGTGAGATTCAGG
CGATGAAGTTCGGCACGCGGTTTGCGGTGCCGCGCCGCGTGCACTCCCTGGACATTAGGGAGGACGGCGTTTTTTGTGCG
ACCCTCGATAATGACCAGATCCTTTGCGCCCGCGCCATCATCGTTGCGACGGGCGTCCAGTATCGCAGGTTGCCGCTCGA
TGGCCTGGAGGAGCTGGAAGGCAGCGGCATCTTTTATGCCGCCACCGAGATGGAGGCGCGCTACTGCAAGGGGGCAGAGG
TCGTCGTCGTGGGCGGCGGGAACTCTGCGGGACAGGCGGCGATGTATCTGTCGCGGTCGGCAAAGCACGTGCACGTTTTG
GTGCGCGGCGACAGTCTTGCCTCATCCATGTCCGACTATTTGTCATCAAGGCTTGAAAATAATCCAACTATTACAATTCA
CTACAAGTCGCAGCTCAAGTCGTTGCATGGCGACGAGGTGATGGAGCGTGTCGTCATTGACCGTGATGGCACGGATTGGG
ACCTGGTCAGTCCCGCCGCTTTTATCATGGTGGGTGCGGCCCCCAACACGAAGTGGCTCGCCGATCATTGTGAGCTGAGT
GAACGGGGCTTTGTGACCACCGGTGCGGATGCCGCCTCGCCCTATGCGACATCCTGCCCGGGCGTGTTTGCCGTGGGCGA
TGTTAGGGCCAACTCCGTGAAACGGGTCGCGTCGGCGGTTGGGGAGGGCTCCGTCGTCGTGTCGCAGGTATGGCAGCACG
TCAACAGCTGA

Upstream 100 bases:

>100_bases
CGCTCCAAATATCTCATCGGTGGGCGTTCTTCGCGCTGAGTGGCCTTTTGCACGGTCTCACCCTACATGTCGTGGCGAAG
GCGTCGTCGATTTAAGGAGA

Downstream 100 bases:

>100_bases
ACGCGACGGGCGACGCGGCGCGCCGATGTGAAGTCTGCTCGGTTCAGGTCCGGGTCACGCGCGTCTCTGATCCGTAGTGG
ATCGCAGCCATCGAGCCCGT

Product: cyclic nucleotide-regulated FAD-dependent pyridine nucleotide-disulfide oxidoreductase

Products: thioredoxin disulfide; NADPH; H+

Alternate protein names: ORF A [H]

Number of amino acids: Translated: 536; Mature: 536

Protein sequence:

>536_residues
MRVESLAGNLREMSRTPLHDSHVEELRQQGDVVRFSKGHIIAEVGDPMDAFIYILEGGVEVIDPYTREAYMPASLGPTQF
MGEIAFLNGGAYSLPMRAKEDTTAIVVPRQKMLDAMARIPEMSDIVITVFAARRRRQIEDGDSSLKLVGMDISRDIRAVA
DFASRNKIPFQILEPGSVDVQETSETCGISLTEPAVIFGHNRVVEDPTPAKVAQLLGLDLEIAEDEVVDVLIVGGGPAGV
AAAVYAGAEGLSAIVVEDVAVGGQAGTSSRIENYLGFPTGISGADLVWRGEIQAMKFGTRFAVPRRVHSLDIREDGVFCA
TLDNDQILCARAIIVATGVQYRRLPLDGLEELEGSGIFYAATEMEARYCKGAEVVVVGGGNSAGQAAMYLSRSAKHVHVL
VRGDSLASSMSDYLSSRLENNPTITIHYKSQLKSLHGDEVMERVVIDRDGTDWDLVSPAAFIMVGAAPNTKWLADHCELS
ERGFVTTGADAASPYATSCPGVFAVGDVRANSVKRVASAVGEGSVVVSQVWQHVNS

Sequences:

>Translated_536_residues
MRVESLAGNLREMSRTPLHDSHVEELRQQGDVVRFSKGHIIAEVGDPMDAFIYILEGGVEVIDPYTREAYMPASLGPTQF
MGEIAFLNGGAYSLPMRAKEDTTAIVVPRQKMLDAMARIPEMSDIVITVFAARRRRQIEDGDSSLKLVGMDISRDIRAVA
DFASRNKIPFQILEPGSVDVQETSETCGISLTEPAVIFGHNRVVEDPTPAKVAQLLGLDLEIAEDEVVDVLIVGGGPAGV
AAAVYAGAEGLSAIVVEDVAVGGQAGTSSRIENYLGFPTGISGADLVWRGEIQAMKFGTRFAVPRRVHSLDIREDGVFCA
TLDNDQILCARAIIVATGVQYRRLPLDGLEELEGSGIFYAATEMEARYCKGAEVVVVGGGNSAGQAAMYLSRSAKHVHVL
VRGDSLASSMSDYLSSRLENNPTITIHYKSQLKSLHGDEVMERVVIDRDGTDWDLVSPAAFIMVGAAPNTKWLADHCELS
ERGFVTTGADAASPYATSCPGVFAVGDVRANSVKRVASAVGEGSVVVSQVWQHVNS
>Mature_536_residues
MRVESLAGNLREMSRTPLHDSHVEELRQQGDVVRFSKGHIIAEVGDPMDAFIYILEGGVEVIDPYTREAYMPASLGPTQF
MGEIAFLNGGAYSLPMRAKEDTTAIVVPRQKMLDAMARIPEMSDIVITVFAARRRRQIEDGDSSLKLVGMDISRDIRAVA
DFASRNKIPFQILEPGSVDVQETSETCGISLTEPAVIFGHNRVVEDPTPAKVAQLLGLDLEIAEDEVVDVLIVGGGPAGV
AAAVYAGAEGLSAIVVEDVAVGGQAGTSSRIENYLGFPTGISGADLVWRGEIQAMKFGTRFAVPRRVHSLDIREDGVFCA
TLDNDQILCARAIIVATGVQYRRLPLDGLEELEGSGIFYAATEMEARYCKGAEVVVVGGGNSAGQAAMYLSRSAKHVHVL
VRGDSLASSMSDYLSSRLENNPTITIHYKSQLKSLHGDEVMERVVIDRDGTDWDLVSPAAFIMVGAAPNTKWLADHCELS
ERGFVTTGADAASPYATSCPGVFAVGDVRANSVKRVASAVGEGSVVVSQVWQHVNS

Specific function: Unknown

COG id: COG0492

COG function: function code O; Thioredoxin reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787114, Length=303, Percent_Identity=31.3531353135314, Blast_Score=128, Evalue=8e-31,
Organism=Escherichia coli, GI87081763, Length=314, Percent_Identity=29.9363057324841, Blast_Score=85, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6320560, Length=324, Percent_Identity=30.2469135802469, Blast_Score=127, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6321898, Length=313, Percent_Identity=30.0319488817891, Blast_Score=125, Evalue=2e-29,

Paralogues:

None

Copy number: 808 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.8.1.9

Molecular weight: Translated: 57514; Mature: 57514

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS50042 CNMP_BINDING_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVESLAGNLREMSRTPLHDSHVEELRQQGDVVRFSKGHIIAEVGDPMDAFIYILEGGVE
CCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEECCCEEEECCCCHHHEEEEEECCCE
VIDPYTREAYMPASLGPTQFMGEIAFLNGGAYSLPMRAKEDTTAIVVPRQKMLDAMARIP
EECCCCCCCCCCCCCCHHHHHCEEEEECCCEEECCCCCCCCCEEEEECHHHHHHHHHHCC
EMSDIVITVFAARRRRQIEDGDSSLKLVGMDISRDIRAVADFASRNKIPFQILEPGSVDV
CCHHHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHHHHHHHCCCCCEEEECCCCCCH
QETSETCGISLTEPAVIFGHNRVVEDPTPAKVAQLLGLDLEIAEDEVVDVLIVGGGPAGV
HCCCCCCCEEECCCEEEEECCEEECCCCHHHHHHHHCCCEEECCCCEEEEEEECCCCHHH
AAAVYAGAEGLSAIVVEDVAVGGQAGTSSRIENYLGFPTGISGADLVWRGEIQAMKFGTR
HHHHHHCCCCCEEEEEEHHHCCCCCCCHHHHHHHCCCCCCCCCCCEEEECCEEEEECCCE
FAVPRRVHSLDIREDGVFCATLDNDQILCARAIIVATGVQYRRLPLDGLEELEGSGIFYA
ECCCCHHEEECCCCCCEEEEEECCCCEEHHHHHHHHCCCCEEECCCCCHHHHCCCCEEEE
ATEMEARYCKGAEVVVVGGGNSAGQAAMYLSRSAKHVHVLVRGDSLASSMSDYLSSRLEN
EECCHHHHCCCCEEEEEECCCCCCHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHCC
NPTITIHYKSQLKSLHGDEVMERVVIDRDGTDWDLVSPAAFIMVGAAPNTKWLADHCELS
CCEEEEEEHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHCCC
ERGFVTTGADAASPYATSCPGVFAVGDVRANSVKRVASAVGEGSVVVSQVWQHVNS
CCCCEEECCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MRVESLAGNLREMSRTPLHDSHVEELRQQGDVVRFSKGHIIAEVGDPMDAFIYILEGGVE
CCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEECCCEEEECCCCHHHEEEEEECCCE
VIDPYTREAYMPASLGPTQFMGEIAFLNGGAYSLPMRAKEDTTAIVVPRQKMLDAMARIP
EECCCCCCCCCCCCCCHHHHHCEEEEECCCEEECCCCCCCCCEEEEECHHHHHHHHHHCC
EMSDIVITVFAARRRRQIEDGDSSLKLVGMDISRDIRAVADFASRNKIPFQILEPGSVDV
CCHHHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHHHHHHHCCCCCEEEECCCCCCH
QETSETCGISLTEPAVIFGHNRVVEDPTPAKVAQLLGLDLEIAEDEVVDVLIVGGGPAGV
HCCCCCCCEEECCCEEEEECCEEECCCCHHHHHHHHCCCEEECCCCEEEEEEECCCCHHH
AAAVYAGAEGLSAIVVEDVAVGGQAGTSSRIENYLGFPTGISGADLVWRGEIQAMKFGTR
HHHHHHCCCCCEEEEEEHHHCCCCCCCHHHHHHHCCCCCCCCCCCEEEECCEEEEECCCE
FAVPRRVHSLDIREDGVFCATLDNDQILCARAIIVATGVQYRRLPLDGLEELEGSGIFYA
ECCCCHHEEECCCCCCEEEEEECCCCEEHHHHHHHHCCCCEEECCCCCHHHHCCCCEEEE
ATEMEARYCKGAEVVVVGGGNSAGQAAMYLSRSAKHVHVLVRGDSLASSMSDYLSSRLEN
EECCHHHHCCCCEEEEEECCCCCCHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHCC
NPTITIHYKSQLKSLHGDEVMERVVIDRDGTDWDLVSPAAFIMVGAAPNTKWLADHCELS
CCEEEEEEHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHCCC
ERGFVTTGADAASPYATSCPGVFAVGDVRANSVKRVASAVGEGSVVVSQVWQHVNS
CCCCEEECCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: NA

Kcat value (1/min): 1365 [C]

Specific activity: NA

Km value (mM): 0.008 {NADPH}} 0.125 {thioredoxin} 0.002 {thioredoxin}} 0.0017 {thioredoxin}} [C]

Substrates: thioredoxin; NADP+

Specific reaction: thioredoxin + NADP+ = thioredoxin disulfide + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: Ca2+; N-Ethyl maleimide; NADP+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1637309 [H]