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The map label for this gene is aglA [H]

Identifier: 89054440

GI number: 89054440

Start: 1941091

End: 1942752

Strand: Direct

Name: aglA [H]

Synonym: Jann_1949

Alternate gene names: 89054440

Gene position: 1941091-1942752 (Clockwise)

Preceding gene: 89054439

Following gene: 89054441

Centisome position: 44.95

GC content: 62.33

Gene sequence:

>1662_bases
ATGACCAAGATGCAGAACATGACACCGGATCAGATCCTGGCCCACGACGCCGATTGGTGGCGCGGCGCGGTGATCTATCA
GATCTATCCGCGCAGCTATCAGGACAGCAACGGCGACGGGATCGGCGACCTGAACGGGATCACCCAGCGCCTGTCGCATA
TCCAGTCGCTTGGCGTGGATGCGATCTGGATCTCACCGTTCTTCACCTCTCCGATGAAGGATTTCGGCTACGACGTCAGT
GATTATTGCGATGTCGACCCGATGTTCGGATCGCTCAGCGATTTCGACGTGCTGATCGAGACGGCCCATGCCCTTGGGCT
GAAGGTGATGATCGATCTGGTGCTGTCCCACACCTCGGACGTGCATCCGTGGTTCATCGAAAGCCGGGCGGACCGCGACA
ATGAGCGGGCCGATTGGTTTGTCTGGGCCGAGCCGAAGGAAGACGGCACGCCGCCCAACAATTGGTTGTCGATTTTCGGC
GGCTCTGCCTGGCAATGGGACGCGCGGCGCGAGCAATATTACCTGCACAATTTCCTGACCTCCCAGCCGGACCTGAACTT
CCATTGTCCGGCGGTGCAGGATGCGCTTCTGGATGTGGTGCGCTTCTGGCTGGATCGCGGGGTGGATGGCTTCCGCCTCG
ATACGATCAACTTCTACTTCCACGATGATGAGATGCGCTCGAACCCGGCGCTGCCCAAGGACAAGCGCGACGACACCATC
GCGCCCTCGGTGAACCCGTATAACCACCAGCTGCACGTCTATTCCAAGAACCAGCCGGAGAACCTGGACTTCCTCAAACG
CTTCCGCGCCGTGCTGGATGAATATCCCGCCGCCACGGCTGTGGGGGAGGTCGGTGACGCCCAGTTCGGGCTGGAGATCA
TGGGCCAGTACACGGCGGGCGATGACATGGTCCACATGTGCTACAGCTTCGAGTTTCTGGCCAAGGATCGCCCCACCGCG
GCGCGGTTTGCCGATGTCATGACCCGGATTGACGAGGCCGCCCCCGATGGATGGGCCTGCCTCGCGTTTTCCAACCACGA
CGTGATGCGCCACGCGACCCGGTGGGAGTTGCCGCCGGCGGCGATGCGGGCGCTGACGACATTGATGATGTGTTTGCGCG
GCTCCCTCTGCCTCTATCAGGGCGAGGAATTGGGCCTGCACGAGGCCGACGTGCCGTTCGAGGCGCTGCAAGATCCCTAT
GGCATCGAGTTCTGGCCTGAATTCAAGGGCCGCGACGGGTGCCGCACGCCGATGGTGTGGGAACCCTCAAACCAGAACGG
TGGCTTTTCCAGCGCGCAGCCCTGGCTACCGGTCAGCCACGAACATCTGACCCAATCGGTGGCCGCGCAGGAAGCGGATC
CCGGATCGCTGCTGCACCATTACCGCCGGGCCATCGGCTTCCGCCATGCGCATAATGCCCTGCTGAAGGGGGCCCATGCG
GATGTGCAGGCCACCGGCGATGTGTTGCACTTCACCCGGACCGACGGCGACGAGACGTTGTTTATCGCAATCAACCTCTC
CGGTGAGCCTGCGGCCATCGACGCGCCAGCAGGCAATTGGGTGCAAATCGGACAAGAACTCGGATCGACCGGCCCTGCGC
CGGACGGAAAATATCACCTCGGCCCATGGCAGCCTGCGCTTGCGCTGCGCGTGGCGGGCTAA

Upstream 100 bases:

>100_bases
GTGGGATTGCGCCGCTTCGCTGTGGCGCGCGACACCTCCTCGCGGCTTCGCCCGCTCCTCGCAGCCGTTATGTGACAGCG
TTCCCGATGAGACGACCTTC

Downstream 100 bases:

>100_bases
CACGAAGGGGACAAACCATGGCGAACCTGAAACTCACCGATGTCGAAAAGACGTATGGCGGAACCGTGCAGGTGCTGCGC
GACATCAACCTGGACATTGA

Product: alpha amylase protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 553; Mature: 552

Protein sequence:

>553_residues
MTKMQNMTPDQILAHDADWWRGAVIYQIYPRSYQDSNGDGIGDLNGITQRLSHIQSLGVDAIWISPFFTSPMKDFGYDVS
DYCDVDPMFGSLSDFDVLIETAHALGLKVMIDLVLSHTSDVHPWFIESRADRDNERADWFVWAEPKEDGTPPNNWLSIFG
GSAWQWDARREQYYLHNFLTSQPDLNFHCPAVQDALLDVVRFWLDRGVDGFRLDTINFYFHDDEMRSNPALPKDKRDDTI
APSVNPYNHQLHVYSKNQPENLDFLKRFRAVLDEYPAATAVGEVGDAQFGLEIMGQYTAGDDMVHMCYSFEFLAKDRPTA
ARFADVMTRIDEAAPDGWACLAFSNHDVMRHATRWELPPAAMRALTTLMMCLRGSLCLYQGEELGLHEADVPFEALQDPY
GIEFWPEFKGRDGCRTPMVWEPSNQNGGFSSAQPWLPVSHEHLTQSVAAQEADPGSLLHHYRRAIGFRHAHNALLKGAHA
DVQATGDVLHFTRTDGDETLFIAINLSGEPAAIDAPAGNWVQIGQELGSTGPAPDGKYHLGPWQPALALRVAG

Sequences:

>Translated_553_residues
MTKMQNMTPDQILAHDADWWRGAVIYQIYPRSYQDSNGDGIGDLNGITQRLSHIQSLGVDAIWISPFFTSPMKDFGYDVS
DYCDVDPMFGSLSDFDVLIETAHALGLKVMIDLVLSHTSDVHPWFIESRADRDNERADWFVWAEPKEDGTPPNNWLSIFG
GSAWQWDARREQYYLHNFLTSQPDLNFHCPAVQDALLDVVRFWLDRGVDGFRLDTINFYFHDDEMRSNPALPKDKRDDTI
APSVNPYNHQLHVYSKNQPENLDFLKRFRAVLDEYPAATAVGEVGDAQFGLEIMGQYTAGDDMVHMCYSFEFLAKDRPTA
ARFADVMTRIDEAAPDGWACLAFSNHDVMRHATRWELPPAAMRALTTLMMCLRGSLCLYQGEELGLHEADVPFEALQDPY
GIEFWPEFKGRDGCRTPMVWEPSNQNGGFSSAQPWLPVSHEHLTQSVAAQEADPGSLLHHYRRAIGFRHAHNALLKGAHA
DVQATGDVLHFTRTDGDETLFIAINLSGEPAAIDAPAGNWVQIGQELGSTGPAPDGKYHLGPWQPALALRVAG
>Mature_552_residues
TKMQNMTPDQILAHDADWWRGAVIYQIYPRSYQDSNGDGIGDLNGITQRLSHIQSLGVDAIWISPFFTSPMKDFGYDVSD
YCDVDPMFGSLSDFDVLIETAHALGLKVMIDLVLSHTSDVHPWFIESRADRDNERADWFVWAEPKEDGTPPNNWLSIFGG
SAWQWDARREQYYLHNFLTSQPDLNFHCPAVQDALLDVVRFWLDRGVDGFRLDTINFYFHDDEMRSNPALPKDKRDDTIA
PSVNPYNHQLHVYSKNQPENLDFLKRFRAVLDEYPAATAVGEVGDAQFGLEIMGQYTAGDDMVHMCYSFEFLAKDRPTAA
RFADVMTRIDEAAPDGWACLAFSNHDVMRHATRWELPPAAMRALTTLMMCLRGSLCLYQGEELGLHEADVPFEALQDPYG
IEFWPEFKGRDGCRTPMVWEPSNQNGGFSSAQPWLPVSHEHLTQSVAAQEADPGSLLHHYRRAIGFRHAHNALLKGAHAD
VQATGDVLHFTRTDGDETLFIAINLSGEPAAIDAPAGNWVQIGQELGSTGPAPDGKYHLGPWQPALALRVAG

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=518, Percent_Identity=33.7837837837838, Blast_Score=277, Evalue=2e-74,
Organism=Escherichia coli, GI1790687, Length=562, Percent_Identity=32.5622775800712, Blast_Score=284, Evalue=9e-78,
Organism=Escherichia coli, GI1786604, Length=509, Percent_Identity=22.3968565815324, Blast_Score=90, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI32565753, Length=545, Percent_Identity=25.3211009174312, Blast_Score=122, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI25147709, Length=489, Percent_Identity=24.7443762781186, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6321731, Length=589, Percent_Identity=33.106960950764, Blast_Score=280, Evalue=6e-76,
Organism=Saccharomyces cerevisiae, GI6319776, Length=589, Percent_Identity=33.106960950764, Blast_Score=279, Evalue=8e-76,
Organism=Saccharomyces cerevisiae, GI6322245, Length=595, Percent_Identity=30.0840336134454, Blast_Score=270, Evalue=4e-73,
Organism=Saccharomyces cerevisiae, GI6321726, Length=555, Percent_Identity=32.4324324324324, Blast_Score=267, Evalue=3e-72,
Organism=Saccharomyces cerevisiae, GI6324416, Length=561, Percent_Identity=31.729055258467, Blast_Score=264, Evalue=3e-71,
Organism=Saccharomyces cerevisiae, GI6322241, Length=561, Percent_Identity=32.4420677361854, Blast_Score=263, Evalue=4e-71,
Organism=Saccharomyces cerevisiae, GI6322021, Length=561, Percent_Identity=32.4420677361854, Blast_Score=263, Evalue=4e-71,
Organism=Drosophila melanogaster, GI24583749, Length=519, Percent_Identity=37.3795761078998, Blast_Score=313, Evalue=2e-85,
Organism=Drosophila melanogaster, GI24583747, Length=519, Percent_Identity=37.3795761078998, Blast_Score=313, Evalue=2e-85,
Organism=Drosophila melanogaster, GI24583745, Length=556, Percent_Identity=36.5107913669065, Blast_Score=313, Evalue=3e-85,
Organism=Drosophila melanogaster, GI24586587, Length=523, Percent_Identity=36.1376673040153, Blast_Score=306, Evalue=2e-83,
Organism=Drosophila melanogaster, GI24586599, Length=526, Percent_Identity=33.6501901140684, Blast_Score=303, Evalue=1e-82,
Organism=Drosophila melanogaster, GI24586597, Length=523, Percent_Identity=34.6080305927342, Blast_Score=299, Evalue=4e-81,
Organism=Drosophila melanogaster, GI24586593, Length=509, Percent_Identity=35.5599214145383, Blast_Score=295, Evalue=4e-80,
Organism=Drosophila melanogaster, GI45549022, Length=517, Percent_Identity=32.495164410058, Blast_Score=295, Evalue=6e-80,
Organism=Drosophila melanogaster, GI24586589, Length=518, Percent_Identity=35.3281853281853, Blast_Score=295, Evalue=8e-80,
Organism=Drosophila melanogaster, GI24586591, Length=505, Percent_Identity=34.6534653465347, Blast_Score=292, Evalue=5e-79,
Organism=Drosophila melanogaster, GI221330053, Length=528, Percent_Identity=34.469696969697, Blast_Score=281, Evalue=1e-75,
Organism=Drosophila melanogaster, GI281360393, Length=453, Percent_Identity=32.4503311258278, Blast_Score=215, Evalue=5e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =3.2.1.20 [H]

Molecular weight: Translated: 62145; Mature: 62014

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKMQNMTPDQILAHDADWWRGAVIYQIYPRSYQDSNGDGIGDLNGITQRLSHIQSLGVD
CCCCCCCCHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
AIWISPFFTSPMKDFGYDVSDYCDVDPMFGSLSDFDVLIETAHALGLKVMIDLVLSHTSD
EEEECCHHCCCHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
VHPWFIESRADRDNERADWFVWAEPKEDGTPPNNWLSIFGGSAWQWDARREQYYLHNFLT
CCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCHHEEECCCCCEECCCHHHHHHHHHHHC
SQPDLNFHCPAVQDALLDVVRFWLDRGVDGFRLDTINFYFHDDEMRSNPALPKDKRDDTI
CCCCCCEECCHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCHHCCCCCCCCCCCCCCC
APSVNPYNHQLHVYSKNQPENLDFLKRFRAVLDEYPAATAVGEVGDAQFGLEIMGQYTAG
CCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHEEECCCCC
DDMVHMCYSFEFLAKDRPTAARFADVMTRIDEAAPDGWACLAFSNHDVMRHATRWELPPA
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHCCCCHH
AMRALTTLMMCLRGSLCLYQGEELGLHEADVPFEALQDPYGIEFWPEFKGRDGCRTPMVW
HHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHCCCCCCEECCCCCCCCCCCCCEEE
EPSNQNGGFSSAQPWLPVSHEHLTQSVAAQEADPGSLLHHYRRAIGFRHAHNALLKGAHA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
DVQATGDVLHFTRTDGDETLFIAINLSGEPAAIDAPAGNWVQIGQELGSTGPAPDGKYHL
CCEECCCEEEEEECCCCCEEEEEEEECCCCCEEECCCCCHHHHHHHHCCCCCCCCCCEEC
GPWQPALALRVAG
CCCCCEEEEEECC
>Mature Secondary Structure 
TKMQNMTPDQILAHDADWWRGAVIYQIYPRSYQDSNGDGIGDLNGITQRLSHIQSLGVD
CCCCCCCHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
AIWISPFFTSPMKDFGYDVSDYCDVDPMFGSLSDFDVLIETAHALGLKVMIDLVLSHTSD
EEEECCHHCCCHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
VHPWFIESRADRDNERADWFVWAEPKEDGTPPNNWLSIFGGSAWQWDARREQYYLHNFLT
CCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCHHEEECCCCCEECCCHHHHHHHHHHHC
SQPDLNFHCPAVQDALLDVVRFWLDRGVDGFRLDTINFYFHDDEMRSNPALPKDKRDDTI
CCCCCCEECCHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCHHCCCCCCCCCCCCCCC
APSVNPYNHQLHVYSKNQPENLDFLKRFRAVLDEYPAATAVGEVGDAQFGLEIMGQYTAG
CCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHEEECCCCC
DDMVHMCYSFEFLAKDRPTAARFADVMTRIDEAAPDGWACLAFSNHDVMRHATRWELPPA
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHCCCCHH
AMRALTTLMMCLRGSLCLYQGEELGLHEADVPFEALQDPYGIEFWPEFKGRDGCRTPMVW
HHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHCCCCCCEECCCCCCCCCCCCCEEE
EPSNQNGGFSSAQPWLPVSHEHLTQSVAAQEADPGSLLHHYRRAIGFRHAHNALLKGAHA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
DVQATGDVLHFTRTDGDETLFIAINLSGEPAAIDAPAGNWVQIGQELGSTGPAPDGKYHL
CCEECCCEEEEEECCCCCEEEEEEEECCCCCEEECCCCCHHHHHHHHCCCCCCCCCCEEC
GPWQPALALRVAG
CCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10400573; 11481430 [H]