Click here to switch to the map view.

The map label for this gene is mmsB [H]

Identifier: 89054398

GI number: 89054398

Start: 1895890

End: 1896762

Strand: Reverse

Name: mmsB [H]

Synonym: Jann_1907

Alternate gene names: 89054398

Gene position: 1896762-1895890 (Counterclockwise)

Preceding gene: 89054399

Following gene: 89054396

Centisome position: 43.93

GC content: 66.9

Gene sequence:

>873_bases
ATGAAAATCGGGTTTATCGGCCTGGGGAACATGGGCGCACCGATGGCGGCCAATCTGGCCGCTGCGGGCCATGAGGTAGT
GGGGTTCGATACGGTGGCGGACACGCCCGAGGGTGTGAAGGCTGTGGGATCGGGCGCTGAGGCCGCGTCAGATGCGGACG
TCGTGATCACCATGCTGCCCAACGGTGCGATCCTGCGCGCGGTGGCGGCTGAGATCATCCCGGCGATGGCCCCGGATGCT
GTTTTCGTCGATTGTTCGACGGTGGAGGTGGACGCGGCGCGGGAGGTGGCAGAGCAGGCTGCGGATGCAGGGCTTGGGTG
GCTCGACGCGCCCGTCTCGGGCGGGGTTGGAGGGGCGGCGGGCGGCACGCTGACGTTCATGGTTGGCGGGTCCGAGGACG
CCTTCGCGCGGGTCCAGCCGCTGTTTGACATCATGGGCCAGAAAGCCGTCCTGTGCGGGCCTGCGGGCAATGGGCAGGCG
GCGAAGATCTGCAACAACATGATCCTGGGCGTGACGATGATTGCAACTTGCGAGGCCTTCGCCATGGCCGACAAACTGGG
CCTCGCGCGGCAATCCATGTTTGATGTGGTCTCCACCTCGTCGGGCTACAGCTGGACGATGAACGCCTATTGCCCGGCCC
CCGGCGTGGGGCCCCAGAGCCCGTCGGACAATGATTACCAACCGGGCTTTGCGGCGGAACTGATGCTCAAGGACCTGGGG
CTGGCGCAGGCGGCGGCAGAGGCGGCGGACGCCGACACGCCCATGGGGGCGGCTGCCCTGGCGCTCTACCGGCGATTTGT
GGAGGAGGAAGACGGTAAGGGCCGGGACTTTTCGGCCATGCTACCCCGGTTTGAGGGACGCAGCCGGAGCTAG

Upstream 100 bases:

>100_bases
GTAAGCCGGTGTGGAAGCACAAGGCGCCCGGCGATGTGACATTGGCTGAGGTGGCGGCGATGTTGCGCCCGCTGGGGCCG
GATGAATGGAGGATGAGCAA

Downstream 100 bases:

>100_bases
CCCGCCGGGTCAGGCGGCCACCCGCAGCAGCAGCCGTTCGAGGCGTTGGTAGGACAGTTCCATCCCCTCACCCAAGCCTT
CGCTCACGACCATGTCGCGG

Product: 3-hydroxyisobutyrate dehydrogenase

Products: NA

Alternate protein names: HIBADH [H]

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MKIGFIGLGNMGAPMAANLAAAGHEVVGFDTVADTPEGVKAVGSGAEAASDADVVITMLPNGAILRAVAAEIIPAMAPDA
VFVDCSTVEVDAAREVAEQAADAGLGWLDAPVSGGVGGAAGGTLTFMVGGSEDAFARVQPLFDIMGQKAVLCGPAGNGQA
AKICNNMILGVTMIATCEAFAMADKLGLARQSMFDVVSTSSGYSWTMNAYCPAPGVGPQSPSDNDYQPGFAAELMLKDLG
LAQAAAEAADADTPMGAAALALYRRFVEEEDGKGRDFSAMLPRFEGRSRS

Sequences:

>Translated_290_residues
MKIGFIGLGNMGAPMAANLAAAGHEVVGFDTVADTPEGVKAVGSGAEAASDADVVITMLPNGAILRAVAAEIIPAMAPDA
VFVDCSTVEVDAAREVAEQAADAGLGWLDAPVSGGVGGAAGGTLTFMVGGSEDAFARVQPLFDIMGQKAVLCGPAGNGQA
AKICNNMILGVTMIATCEAFAMADKLGLARQSMFDVVSTSSGYSWTMNAYCPAPGVGPQSPSDNDYQPGFAAELMLKDLG
LAQAAAEAADADTPMGAAALALYRRFVEEEDGKGRDFSAMLPRFEGRSRS
>Mature_290_residues
MKIGFIGLGNMGAPMAANLAAAGHEVVGFDTVADTPEGVKAVGSGAEAASDADVVITMLPNGAILRAVAAEIIPAMAPDA
VFVDCSTVEVDAAREVAEQAADAGLGWLDAPVSGGVGGAAGGTLTFMVGGSEDAFARVQPLFDIMGQKAVLCGPAGNGQA
AKICNNMILGVTMIATCEAFAMADKLGLARQSMFDVVSTSSGYSWTMNAYCPAPGVGPQSPSDNDYQPGFAAELMLKDLG
LAQAAAEAADADTPMGAAALALYRRFVEEEDGKGRDFSAMLPRFEGRSRS

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=289, Percent_Identity=41.8685121107266, Blast_Score=215, Evalue=4e-56,
Organism=Homo sapiens, GI40556376, Length=272, Percent_Identity=27.5735294117647, Blast_Score=111, Evalue=1e-24,
Organism=Escherichia coli, GI145693186, Length=302, Percent_Identity=34.4370860927152, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1786719, Length=296, Percent_Identity=33.1081081081081, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1790315, Length=294, Percent_Identity=31.6326530612245, Blast_Score=117, Evalue=9e-28,
Organism=Escherichia coli, GI1789092, Length=296, Percent_Identity=30.0675675675676, Blast_Score=98, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17557316, Length=283, Percent_Identity=40.2826855123675, Blast_Score=191, Evalue=4e-49,
Organism=Drosophila melanogaster, GI24655230, Length=285, Percent_Identity=41.0526315789474, Blast_Score=219, Evalue=2e-57,
Organism=Drosophila melanogaster, GI19922568, Length=285, Percent_Identity=41.0526315789474, Blast_Score=219, Evalue=2e-57,
Organism=Drosophila melanogaster, GI28574115, Length=272, Percent_Identity=23.1617647058824, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR011548
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: =1.1.1.31 [H]

Molecular weight: Translated: 29409; Mature: 29409

Theoretical pI: Translated: 4.02; Mature: 4.02

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGFIGLGNMGAPMAANLAAAGHEVVGFDTVADTPEGVKAVGSGAEAASDADVVITMLP
CEEEEEECCCCCCCHHHHHHHCCCCEEECCCCCCCCHHHHHHCCCCCCCCCCCEEEEECC
NGAILRAVAAEIIPAMAPDAVFVDCSTVEVDAAREVAEQAADAGLGWLDAPVSGGVGGAA
CCHHHHHHHHHHHHCCCCCEEEEECCEEEHHHHHHHHHHHHHCCCCEECCCCCCCCCCCC
GGTLTFMVGGSEDAFARVQPLFDIMGQKAVLCGPAGNGQAAKICNNMILGVTMIATCEAF
CCEEEEEEECCCCHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
AMADKLGLARQSMFDVVSTSSGYSWTMNAYCPAPGVGPQSPSDNDYQPGFAAELMLKDLG
HHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
LAQAAAEAADADTPMGAAALALYRRFVEEEDGKGRDFSAMLPRFEGRSRS
HHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCC
>Mature Secondary Structure
MKIGFIGLGNMGAPMAANLAAAGHEVVGFDTVADTPEGVKAVGSGAEAASDADVVITMLP
CEEEEEECCCCCCCHHHHHHHCCCCEEECCCCCCCCHHHHHHCCCCCCCCCCCEEEEECC
NGAILRAVAAEIIPAMAPDAVFVDCSTVEVDAAREVAEQAADAGLGWLDAPVSGGVGGAA
CCHHHHHHHHHHHHCCCCCEEEEECCEEEHHHHHHHHHHHHHCCCCEECCCCCCCCCCCC
GGTLTFMVGGSEDAFARVQPLFDIMGQKAVLCGPAGNGQAAKICNNMILGVTMIATCEAF
CCEEEEEEECCCCHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
AMADKLGLARQSMFDVVSTSSGYSWTMNAYCPAPGVGPQSPSDNDYQPGFAAELMLKDLG
HHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
LAQAAAEAADADTPMGAAALALYRRFVEEEDGKGRDFSAMLPRFEGRSRS
HHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]