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The map label for this gene is ykfB [H]

Identifier: 89053899

GI number: 89053899

Start: 1377568

End: 1378683

Strand: Direct

Name: ykfB [H]

Synonym: Jann_1408

Alternate gene names: 89053899

Gene position: 1377568-1378683 (Clockwise)

Preceding gene: 89053897

Following gene: 89053901

Centisome position: 31.9

GC content: 64.43

Gene sequence:

>1116_bases
ATGTCCGATCAGTCCCAAATCATCGCCGGCTTCACGCTCTGGCATCTTTCGTTGCCCGTGACCGCGCGGCGCGACCATGG
CATCGGGTCTGTCGCCGGTGCGGTCGAGGTGGTGGTGTTGCGGTTGCAGGCCGACAGCGGTGCGGTCGGCTATGGAGAGG
CCTCGCCCTGGGTGGTGTTCACCGGCTCTGTGGAGGCGACCTATGCCGCGCTCGACCGATATCTGCGGCCCTTGGTTCTG
GGCCGCGCGGTGGGTGATCACGCGGCGATCATGGAGGATGCCCGCGCCGCCGTTGCCCATTGCACGGAGGCCAAAGCGGC
CCTTGATACGGCGCTATATGACCTGCGCGCACGGATCGCCGGGGTGCCTGTCTGGGCGCTTCTGGGCGGGCGGTGCCGGG
ATCGCATCCCGCTCAGCTGTTCCATCGCTGATCCTGATTTCGATAAGGATCTGGCCCTGATGCAGCGCTTGCAGGATGAC
GATGTGCGCATCATCAAGCTGAAGACAGGCTTCAAGGACCACGCCTTCGACATGATGCGGCTGGAGCGATTGCGCGCCGA
CTTTCCCGCGTTCGACATTCGTGTGGACTACAATCAGGGACTGCATCACGACGTCGCCTTGGCCCGTGTGCGCGACGTTG
CCACCTTCAAGCCCACGTTCATCGAGCAACCCGTGAAGGCCCATCTGCGGGGCCTCATGGCGCGGATCCGCGATGCGGTG
GACGTGCCGCTACTGGCCGATGAGAGCATTTTTGGCCCCGAGGATATGGCGGAGCATCCAGAGATTGCCGACGGCGTCTC
GATCAAGATCATGAAGTCCGGTGGTCTGACCCGGGCCCAAACCGTGGCGCGGATGGCGGCGGCGCGGGGGCTGTCAGCCT
ATGGTGGCGACATGTTCGAGGCGGGTCTGGCCCATCTGGCAGGCGCGCATATGATCGCGGCGACGCCGGAAATCACCCTG
GGGTGTGAGTTCTATCAGGCCACGTATTTCCTATGCGACGATATCCTCGCGGCCCCGTTTCCAGTGGCCGATGGGCATGT
CTTGGTGCCCGATACTCCGGGTCTCGGGGTGGATGTGGACGAGGACGCGCTTGCGCGCTTCGCGGTGGCGCGATGA

Upstream 100 bases:

>100_bases
ATGGCATCAAGTATATCACTTTTTCTGATGTCTTGCGAAGAATTGCATATTTGAAATGATGAGTTTTCCCGTCCAACGTG
CATGGGCTTGGGGGAGCAAT

Downstream 100 bases:

>100_bases
CCTGGTCAGTACGTCGCCGCGGCATACCATTAATAGGTCGCTGCCGCATACCATTAATAGGTCGCTGCCGCTCTGGCCGC
CTGATCATACTGCCCGGCCA

Product: muconate cycloisomerase

Products: Glyceraldehyde 3-phosphate; Pyruvate [C]

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MSDQSQIIAGFTLWHLSLPVTARRDHGIGSVAGAVEVVVLRLQADSGAVGYGEASPWVVFTGSVEATYAALDRYLRPLVL
GRAVGDHAAIMEDARAAVAHCTEAKAALDTALYDLRARIAGVPVWALLGGRCRDRIPLSCSIADPDFDKDLALMQRLQDD
DVRIIKLKTGFKDHAFDMMRLERLRADFPAFDIRVDYNQGLHHDVALARVRDVATFKPTFIEQPVKAHLRGLMARIRDAV
DVPLLADESIFGPEDMAEHPEIADGVSIKIMKSGGLTRAQTVARMAAARGLSAYGGDMFEAGLAHLAGAHMIAATPEITL
GCEFYQATYFLCDDILAAPFPVADGHVLVPDTPGLGVDVDEDALARFAVAR

Sequences:

>Translated_371_residues
MSDQSQIIAGFTLWHLSLPVTARRDHGIGSVAGAVEVVVLRLQADSGAVGYGEASPWVVFTGSVEATYAALDRYLRPLVL
GRAVGDHAAIMEDARAAVAHCTEAKAALDTALYDLRARIAGVPVWALLGGRCRDRIPLSCSIADPDFDKDLALMQRLQDD
DVRIIKLKTGFKDHAFDMMRLERLRADFPAFDIRVDYNQGLHHDVALARVRDVATFKPTFIEQPVKAHLRGLMARIRDAV
DVPLLADESIFGPEDMAEHPEIADGVSIKIMKSGGLTRAQTVARMAAARGLSAYGGDMFEAGLAHLAGAHMIAATPEITL
GCEFYQATYFLCDDILAAPFPVADGHVLVPDTPGLGVDVDEDALARFAVAR
>Mature_370_residues
SDQSQIIAGFTLWHLSLPVTARRDHGIGSVAGAVEVVVLRLQADSGAVGYGEASPWVVFTGSVEATYAALDRYLRPLVLG
RAVGDHAAIMEDARAAVAHCTEAKAALDTALYDLRARIAGVPVWALLGGRCRDRIPLSCSIADPDFDKDLALMQRLQDDD
VRIIKLKTGFKDHAFDMMRLERLRADFPAFDIRVDYNQGLHHDVALARVRDVATFKPTFIEQPVKAHLRGLMARIRDAVD
VPLLADESIFGPEDMAEHPEIADGVSIKIMKSGGLTRAQTVARMAAARGLSAYGGDMFEAGLAHLAGAHMIAATPEITLG
CEFYQATYFLCDDILAAPFPVADGHVLVPDTPGLGVDVDEDALARFAVAR

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has probably a role in the metabolism of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of the other Ala-X dipepti

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: 4.1.2.21; 4.2.1.6 [C]

Molecular weight: Translated: 40055; Mature: 39924

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00908 MR_MLE_1 ; PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDQSQIIAGFTLWHLSLPVTARRDHGIGSVAGAVEVVVLRLQADSGAVGYGEASPWVVF
CCCCHHEEEEEEEEEEECCEEECCCCCCHHHHHHEEEEEEEEECCCCCCCCCCCCCEEEE
TGSVEATYAALDRYLRPLVLGRAVGDHAAIMEDARAAVAHCTEAKAALDTALYDLRARIA
ECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GVPVWALLGGRCRDRIPLSCSIADPDFDKDLALMQRLQDDDVRIIKLKTGFKDHAFDMMR
CCCHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHH
LERLRADFPAFDIRVDYNQGLHHDVALARVRDVATFKPTFIEQPVKAHLRGLMARIRDAV
HHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHC
DVPLLADESIFGPEDMAEHPEIADGVSIKIMKSGGLTRAQTVARMAAARGLSAYGGDMFE
CCCEECCCCCCCCHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCHHHH
AGLAHLAGAHMIAATPEITLGCEFYQATYFLCDDILAAPFPVADGHVLVPDTPGLGVDVD
HHHHHHCCCEEEEECCCEEECHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCC
EDALARFAVAR
HHHHHHHHHCC
>Mature Secondary Structure 
SDQSQIIAGFTLWHLSLPVTARRDHGIGSVAGAVEVVVLRLQADSGAVGYGEASPWVVF
CCCHHEEEEEEEEEEECCEEECCCCCCHHHHHHEEEEEEEEECCCCCCCCCCCCCEEEE
TGSVEATYAALDRYLRPLVLGRAVGDHAAIMEDARAAVAHCTEAKAALDTALYDLRARIA
ECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GVPVWALLGGRCRDRIPLSCSIADPDFDKDLALMQRLQDDDVRIIKLKTGFKDHAFDMMR
CCCHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHH
LERLRADFPAFDIRVDYNQGLHHDVALARVRDVATFKPTFIEQPVKAHLRGLMARIRDAV
HHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHC
DVPLLADESIFGPEDMAEHPEIADGVSIKIMKSGGLTRAQTVARMAAARGLSAYGGDMFE
CCCEECCCCCCCCHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCHHHH
AGLAHLAGAHMIAATPEITLGCEFYQATYFLCDDILAAPFPVADGHVLVPDTPGLGVDVD
HHHHHHCCCEEEEECCCEEECHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCC
EDALARFAVAR
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-Dehydro-3-deoxy-D-galactonate 6-phosphate [C]

Specific reaction: 2-Dehydro-3-deoxy-D-galactonate 6-phosphate <==> Glyceraldehyde 3-phosphate + Pyruvate [C]

General reaction: elimination of H2O C-O bond; Cleavage [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]