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The map label for this gene is gutB [H]

Identifier: 89053891

GI number: 89053891

Start: 1370215

End: 1371201

Strand: Direct

Name: gutB [H]

Synonym: Jann_1400

Alternate gene names: 89053891

Gene position: 1370215-1371201 (Clockwise)

Preceding gene: 89053890

Following gene: 89053892

Centisome position: 31.73

GC content: 65.86

Gene sequence:

>987_bases
ATGAAAGCGCTTGTGTACGACGGTCCTGAACAATTGGGTTTCCGCGATGTCGCCGATGCGGTCGCAGGCCCCGGCCAACA
CTTGATCAAGGTCCTGTCCGTGGGCGTTTGCGGGTCAGACATGCACGCCTACCTTGGCCATGATGAGCGTCGGCCCGCGC
CGCTCATCCTGGGCCATGAAGCTGCCGGAGAGATCATCAGCGGCCCCCGCGCCGGAGAGCGTGTGACGGTGAACCCGCTG
GTGTCCTGCGGGTCTTGTGCTGCGTGTTCCAGCGGCCACGACAACCTGTGCGCTGAGCGGATGATCATCTCCATGCCGCC
GCGCGAAGGCGCATTCGCCCAGATGATCGCGATGTGCGATGAGAACCTGGTGACGGTGCCCGATCATGTCTCTTTGGATG
CGGCCTGTCTGGCGGAGCCCATTGCCTGCGGCTGGCACGCGGTGCGGCTGGCGAAGCGCGCTCTGGCCGATGCCCGCACG
GCCCTGGTCATTGGGGGCGGAGCCATCGGTCTTGGCGCGGCCCTGTCATTGGCGGCGCAGGGTGTCACCGAGGTGACGAT
CCTTGAGCCCAACGCGATGCGGCGTGAGGTGCTGAACGGCGCGTGCCAGCAGACGTCCATCGCCCCCGATGACCTGCCCG
CAGACGCCATGTTCGACGTCGTGATTGACGGCGTGGGCTATGCCGCGACCCGGGCCACCGCATCCGCCCATGCGCGGCCC
GGCGGCGTGATTGCGCATATCGGGCTGGGGGAGGCGGCAGGTGGCCTCGATATCCGTCGGATGACCTTGCAAGAGATCAC
CTTTTTCGGCACCTATACCTACACCGCACAGGACTTCCGTGACACGGCAGAGGCCATCTTTGACGGCCGTCTGGGTCCGC
TCGACTGGACCGAGGCGCGCCCGCTCAGCGACGGGGCCAGTGCTTTCACCGACATCCGTGCGGGCAAATCAGCCGCGCCC
AAGATCGTGCTGCGTCCCCATGATTAA

Upstream 100 bases:

>100_bases
CGATGGAAGATATCGACGGGCCGCTTCTGTTCCTGTGCTCAGACGCCTCCTCATTTGTCACGGGCCAAGTTCTGATGGTC
GACGGGGGGTATACGGCGAA

Downstream 100 bases:

>100_bases
CCCAGTCCCCATCTACAGGAGCTTGTGACGCCCGTGAGTATTTCCAAGAAGATCGTTGACGATTTGGTCGCCAACGACGT
GTCCTTCATCACCACCGTGC

Product: alcohol dehydrogenase GroES-like protein

Products: NA

Alternate protein names: Glucitol dehydrogenase; L-iditol 2-dehydrogenase [H]

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MKALVYDGPEQLGFRDVADAVAGPGQHLIKVLSVGVCGSDMHAYLGHDERRPAPLILGHEAAGEIISGPRAGERVTVNPL
VSCGSCAACSSGHDNLCAERMIISMPPREGAFAQMIAMCDENLVTVPDHVSLDAACLAEPIACGWHAVRLAKRALADART
ALVIGGGAIGLGAALSLAAQGVTEVTILEPNAMRREVLNGACQQTSIAPDDLPADAMFDVVIDGVGYAATRATASAHARP
GGVIAHIGLGEAAGGLDIRRMTLQEITFFGTYTYTAQDFRDTAEAIFDGRLGPLDWTEARPLSDGASAFTDIRAGKSAAP
KIVLRPHD

Sequences:

>Translated_328_residues
MKALVYDGPEQLGFRDVADAVAGPGQHLIKVLSVGVCGSDMHAYLGHDERRPAPLILGHEAAGEIISGPRAGERVTVNPL
VSCGSCAACSSGHDNLCAERMIISMPPREGAFAQMIAMCDENLVTVPDHVSLDAACLAEPIACGWHAVRLAKRALADART
ALVIGGGAIGLGAALSLAAQGVTEVTILEPNAMRREVLNGACQQTSIAPDDLPADAMFDVVIDGVGYAATRATASAHARP
GGVIAHIGLGEAAGGLDIRRMTLQEITFFGTYTYTAQDFRDTAEAIFDGRLGPLDWTEARPLSDGASAFTDIRAGKSAAP
KIVLRPHD
>Mature_328_residues
MKALVYDGPEQLGFRDVADAVAGPGQHLIKVLSVGVCGSDMHAYLGHDERRPAPLILGHEAAGEIISGPRAGERVTVNPL
VSCGSCAACSSGHDNLCAERMIISMPPREGAFAQMIAMCDENLVTVPDHVSLDAACLAEPIACGWHAVRLAKRALADART
ALVIGGGAIGLGAALSLAAQGVTEVTILEPNAMRREVLNGACQQTSIAPDDLPADAMFDVVIDGVGYAATRATASAHARP
GGVIAHIGLGEAAGGLDIRRMTLQEITFFGTYTYTAQDFRDTAEAIFDGRLGPLDWTEARPLSDGASAFTDIRAGKSAAP
KIVLRPHD

Specific function: Reduces glucitol to fructose [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=293, Percent_Identity=25.2559726962457, Blast_Score=89, Evalue=5e-18,
Organism=Escherichia coli, GI226510992, Length=306, Percent_Identity=30.0653594771242, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1787863, Length=307, Percent_Identity=26.0586319218241, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1788073, Length=290, Percent_Identity=28.2758620689655, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1790045, Length=347, Percent_Identity=27.3775216138329, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1790718, Length=321, Percent_Identity=24.6105919003115, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1788407, Length=200, Percent_Identity=31, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788075, Length=341, Percent_Identity=24.0469208211144, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87082125, Length=353, Percent_Identity=25.4957507082153, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17562876, Length=348, Percent_Identity=27.2988505747126, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17562878, Length=204, Percent_Identity=29.4117647058824, Blast_Score=95, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6319257, Length=383, Percent_Identity=28.4595300261097, Blast_Score=129, Evalue=6e-31,
Organism=Saccharomyces cerevisiae, GI6319258, Length=351, Percent_Identity=26.2108262108262, Blast_Score=103, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6322619, Length=331, Percent_Identity=25.0755287009063, Blast_Score=99, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6323099, Length=335, Percent_Identity=24.7761194029851, Blast_Score=99, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6319955, Length=331, Percent_Identity=25.0755287009063, Blast_Score=99, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6323980, Length=130, Percent_Identity=30.7692307692308, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI17737897, Length=326, Percent_Identity=29.4478527607362, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI17137530, Length=180, Percent_Identity=32.7777777777778, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.14 [H]

Molecular weight: Translated: 34142; Mature: 34142

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKALVYDGPEQLGFRDVADAVAGPGQHLIKVLSVGVCGSDMHAYLGHDERRPAPLILGHE
CCCEEECCHHHCCHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCEEECCH
AAGEIISGPRAGERVTVNPLVSCGSCAACSSGHDNLCAERMIISMPPREGAFAQMIAMCD
HCCHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHEEECCCCCCHHHHHHHHHC
ENLVTVPDHVSLDAACLAEPIACGWHAVRLAKRALADARTALVIGGGAIGLGAALSLAAQ
CCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHC
GVTEVTILEPNAMRREVLNGACQQTSIAPDDLPADAMFDVVIDGVGYAATRATASAHARP
CCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCC
GGVIAHIGLGEAAGGLDIRRMTLQEITFFGTYTYTAQDFRDTAEAIFDGRLGPLDWTEAR
CCEEEEECCCCCCCCCHHHHHHHHHHHEEEEEEEEHHHHHHHHHHHHCCCCCCCCCCCCC
PLSDGASAFTDIRAGKSAAPKIVLRPHD
CCCCCHHHHHHHHCCCCCCCEEEEECCC
>Mature Secondary Structure
MKALVYDGPEQLGFRDVADAVAGPGQHLIKVLSVGVCGSDMHAYLGHDERRPAPLILGHE
CCCEEECCHHHCCHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCEEECCH
AAGEIISGPRAGERVTVNPLVSCGSCAACSSGHDNLCAERMIISMPPREGAFAQMIAMCD
HCCHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHEEECCCCCCHHHHHHHHHC
ENLVTVPDHVSLDAACLAEPIACGWHAVRLAKRALADARTALVIGGGAIGLGAALSLAAQ
CCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHC
GVTEVTILEPNAMRREVLNGACQQTSIAPDDLPADAMFDVVIDGVGYAATRATASAHARP
CCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCC
GGVIAHIGLGEAAGGLDIRRMTLQEITFFGTYTYTAQDFRDTAEAIFDGRLGPLDWTEAR
CCEEEEECCCCCCCCCHHHHHHHHHHHEEEEEEEEHHHHHHHHHHHHCCCCCCCCCCCCC
PLSDGASAFTDIRAGKSAAPKIVLRPHD
CCCCCHHHHHHHHCCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1460002; 9384377; 8195086 [H]