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The map label for this gene is mcbR [H]

Identifier: 89053883

GI number: 89053883

Start: 1362186

End: 1362860

Strand: Direct

Name: mcbR [H]

Synonym: Jann_1392

Alternate gene names: 89053883

Gene position: 1362186-1362860 (Clockwise)

Preceding gene: 89053876

Following gene: 89053884

Centisome position: 31.55

GC content: 62.96

Gene sequence:

>675_bases
ATGGGTGCCATGGCGAAAACTTCCGATCAGGCTTTGGGCATGACGCGCGGGTCGACCGTGGGCCGGATTGAGGCCGCGTT
GACGCAAGCGATCTCGGCGGGAGAACTTGCACCCGGCGAGCGCATTGACGAGGTCAAGCTGACCGAGCGGTTCGGCGTGT
CCCGGACCCCGGTGCGCGAGGCGTTGTCGCGTCTGGTGGCGCAGGGGATCCTCATTCCCGGAGAGAAGCGCGGCGTTCGC
GTTGCAGAGTATACGCGAGAGCAATTGGCCCAGATTTTTGAAGCAATGCACGAGATTGAGGCAGCATGCGCCCGTGTCGC
GGCGCAGCGCCTGACCCTTCTGGCGCGGGTCGAGATCGAGGGGGCGCAGGCCGAGTGTATGGCCGCCGCTGAGTCGGGCG
ACCGCAGTGCCTACCTGAAGGCCAATGAGGCGTTTCACCTGACGATCTACCGTGCGACCGGCAACCCCTACATGGCCGAG
ATCGCGTCTGAATTCCGCAATCGAACGGGCGCTGCACGGGCCAAAAAATTTGCCACGCCCGAAGATCTTCTAACTTCCGC
CCATAGTCATGCAGACCTGATCTCGCTGATTTTCTCGGAGGATTCGGATGCCGCATCGGACGGTATGAGGGCCCATATGG
CGCAGAGTTTCCGCGCGACGCTGGCCGCCAACTAG

Upstream 100 bases:

>100_bases
GTCCCGTCACAGCATTCTGTATGCAAACACGTTTGTAAATATCCAAAATTGAAGTTTCCCGATGAATGATGTAGGTGAGG
AGTCGAACAACAGGGAAGGC

Downstream 100 bases:

>100_bases
GGTCGCTGGTATTTTTCGGCTTGCATTCGCACGCAAGGATGTCACTTTGCATACAAACGTGACTCGACAAGCGAATGGAG
GCCCCAATGGGCGTACAAGA

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MGAMAKTSDQALGMTRGSTVGRIEAALTQAISAGELAPGERIDEVKLTERFGVSRTPVREALSRLVAQGILIPGEKRGVR
VAEYTREQLAQIFEAMHEIEAACARVAAQRLTLLARVEIEGAQAECMAAAESGDRSAYLKANEAFHLTIYRATGNPYMAE
IASEFRNRTGAARAKKFATPEDLLTSAHSHADLISLIFSEDSDAASDGMRAHMAQSFRATLAAN

Sequences:

>Translated_224_residues
MGAMAKTSDQALGMTRGSTVGRIEAALTQAISAGELAPGERIDEVKLTERFGVSRTPVREALSRLVAQGILIPGEKRGVR
VAEYTREQLAQIFEAMHEIEAACARVAAQRLTLLARVEIEGAQAECMAAAESGDRSAYLKANEAFHLTIYRATGNPYMAE
IASEFRNRTGAARAKKFATPEDLLTSAHSHADLISLIFSEDSDAASDGMRAHMAQSFRATLAAN
>Mature_223_residues
GAMAKTSDQALGMTRGSTVGRIEAALTQAISAGELAPGERIDEVKLTERFGVSRTPVREALSRLVAQGILIPGEKRGVRV
AEYTREQLAQIFEAMHEIEAACARVAAQRLTLLARVEIEGAQAECMAAAESGDRSAYLKANEAFHLTIYRATGNPYMAEI
ASEFRNRTGAARAKKFATPEDLLTSAHSHADLISLIFSEDSDAASDGMRAHMAQSFRATLAAN

Specific function: Important for biofilm formation. Represses expression of mcbA by binding to its promoter region, which prevents colanic acid overproduction and mucoidy [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081914, Length=206, Percent_Identity=26.6990291262136, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI48994961, Length=205, Percent_Identity=27.3170731707317, Blast_Score=68, Evalue=4e-13,
Organism=Escherichia coli, GI87082149, Length=197, Percent_Identity=25.8883248730964, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 24056; Mature: 23924

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAMAKTSDQALGMTRGSTVGRIEAALTQAISAGELAPGERIDEVKLTERFGVSRTPVRE
CCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCHHHH
ALSRLVAQGILIPGEKRGVRVAEYTREQLAQIFEAMHEIEAACARVAAQRLTLLARVEIE
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEC
GAQAECMAAAESGDRSAYLKANEAFHLTIYRATGNPYMAEIASEFRNRTGAARAKKFATP
CCHHHHHHHHCCCCCCEEEEECCEEEEEEEEECCCCHHHHHHHHHHHCCCHHHHHHCCCH
EDLLTSAHSHADLISLIFSEDSDAASDGMRAHMAQSFRATLAAN
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GAMAKTSDQALGMTRGSTVGRIEAALTQAISAGELAPGERIDEVKLTERFGVSRTPVRE
CCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCHHHH
ALSRLVAQGILIPGEKRGVRVAEYTREQLAQIFEAMHEIEAACARVAAQRLTLLARVEIE
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEC
GAQAECMAAAESGDRSAYLKANEAFHLTIYRATGNPYMAEIASEFRNRTGAARAKKFATP
CCHHHHHHHHCCCCCCEEEEECCEEEEEEEEECCCCHHHHHHHHHHHCCCHHHHHHCCCH
EDLLTSAHSHADLISLIFSEDSDAASDGMRAHMAQSFRATLAAN
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]