Click here to switch to the map view.

The map label for this gene is potD [C]

Identifier: 89053854

GI number: 89053854

Start: 1327386

End: 1328408

Strand: Direct

Name: potD [C]

Synonym: Jann_1363

Alternate gene names: 89053854

Gene position: 1327386-1328408 (Clockwise)

Preceding gene: 89053852

Following gene: 89053855

Centisome position: 30.74

GC content: 64.13

Gene sequence:

>1023_bases
ATGCGGACAACGAACCTAACGGCCATCCTGGTCGCCACGACGAGCTTATACGTGACCGCGCCGACGGCTGCGCAGGCCGA
TGAGCTGGTCCTATATTGCTCGGTGCAAGAGGAATGGTGCCGCGCGATGTCCGAGGCGTTTGAGCGAGAGACCGGGATCG
AGGTCCTGATGACCCGCCGGTCGTCCGGCGAGACCTTCGCGCAACTTGCCGCAGAGGCCGAACAGCCCCGTGGCGACGTG
TGGTGGGGCGGCACAGGTGACCCGCATCTGCAAGCCGCGCAAGAGGGTTTGACCGAGGAATATCAGTCCGAAATGTTGGG
CAACTTGCAGGATTGGGCCGTGCGTCAGGCCGAAACCGCGAACTACCGCACCGTTGGCATCTACGCCGGTGGGTTGGGCT
TCGGCTATAATTCCGATCTTGTAGACGGCGATGCGCCTGCGTGTTGGGCCGATCTGCTGGATGAACGCTTTGCCGATGAC
GTGCAGGTCGCCAACCCCAATTCGTCCGGCACATCCTACACGATGCTCGCCACGATGGTGCAGCTGATGGGCGAGGATGA
GGCCTTCGCCTACCTGGCCGAATTGCACAACAACATCAGCCAGTACACGCAATCGGGCTCCGCTCCGATCCGCGCGGCAG
CCACCGGCGAAACAGCCGTTGGCATCGTCTTCATGCACGATGCGGTGGCGCAGACGGTGCAGGGCGCGCCGATCGTGGCC
GTGGCGCCGTGCGAAGGGACGGGGTATGAAGTCGGCTCCATGTCGATCATCGCGGGCGGTCCGAACCCGGACAGTGCGCG
CACCTTCTACGACTGGGCCCTGACGCCCGAAGCGCAGGAGATCGGGGCGGCAAACAACAGCTTCCAGATCCCGTCGAACC
TCGATTCCGCCACCCCCGAGGCCTCCCCGCGGCTGGAGGACATCACGCTGATCGACTACGACTTCGCGCTCTACGGCTCG
TCCGAGGAACGCACCCGTCTGTTGCAGCGCTGGGACGCTGACATCGGCTCGCTCGCGCAGTAA

Upstream 100 bases:

>100_bases
CGGGGCATCAAACGCAGTCTTCGACAAATCTGTTGAAGATTGCACGACTTGCAGCGCTAATGGTTTTCGGGAGAATCTGC
TTACATCTTGGGAGGATACA

Downstream 100 bases:

>100_bases
AACGTGCGATTTGCCCTGACATCTGACCGAGCTGGCGTGGCTTGGCTCGGTCTGCTCGCCCTCTCGCTGGTGGCGTTTCC
CTGGTACAAGGGCGCGTCTT

Product: extracellular solute-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MRTTNLTAILVATTSLYVTAPTAAQADELVLYCSVQEEWCRAMSEAFERETGIEVLMTRRSSGETFAQLAAEAEQPRGDV
WWGGTGDPHLQAAQEGLTEEYQSEMLGNLQDWAVRQAETANYRTVGIYAGGLGFGYNSDLVDGDAPACWADLLDERFADD
VQVANPNSSGTSYTMLATMVQLMGEDEAFAYLAELHNNISQYTQSGSAPIRAAATGETAVGIVFMHDAVAQTVQGAPIVA
VAPCEGTGYEVGSMSIIAGGPNPDSARTFYDWALTPEAQEIGAANNSFQIPSNLDSATPEASPRLEDITLIDYDFALYGS
SEERTRLLQRWDADIGSLAQ

Sequences:

>Translated_340_residues
MRTTNLTAILVATTSLYVTAPTAAQADELVLYCSVQEEWCRAMSEAFERETGIEVLMTRRSSGETFAQLAAEAEQPRGDV
WWGGTGDPHLQAAQEGLTEEYQSEMLGNLQDWAVRQAETANYRTVGIYAGGLGFGYNSDLVDGDAPACWADLLDERFADD
VQVANPNSSGTSYTMLATMVQLMGEDEAFAYLAELHNNISQYTQSGSAPIRAAATGETAVGIVFMHDAVAQTVQGAPIVA
VAPCEGTGYEVGSMSIIAGGPNPDSARTFYDWALTPEAQEIGAANNSFQIPSNLDSATPEASPRLEDITLIDYDFALYGS
SEERTRLLQRWDADIGSLAQ
>Mature_340_residues
MRTTNLTAILVATTSLYVTAPTAAQADELVLYCSVQEEWCRAMSEAFERETGIEVLMTRRSSGETFAQLAAEAEQPRGDV
WWGGTGDPHLQAAQEGLTEEYQSEMLGNLQDWAVRQAETANYRTVGIYAGGLGFGYNSDLVDGDAPACWADLLDERFADD
VQVANPNSSGTSYTMLATMVQLMGEDEAFAYLAELHNNISQYTQSGSAPIRAAATGETAVGIVFMHDAVAQTVQGAPIVA
VAPCEGTGYEVGSMSIIAGGPNPDSARTFYDWALTPEAQEIGAANNSFQIPSNLDSATPEASPRLEDITLIDYDFALYGS
SEERTRLLQRWDADIGSLAQ

Specific function: Required For The Activity Of The Bacterial Periplasmic Transport System Of Putrescine And Spermidine. Polyamine Binding Protein. [C]

COG id: COG1840

COG function: function code P; ABC-type Fe3+ transport system, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 36576; Mature: 36576

Theoretical pI: Translated: 3.79; Mature: 3.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTTNLTAILVATTSLYVTAPTAAQADELVLYCSVQEEWCRAMSEAFERETGIEVLMTRR
CCCCCEEEEEEEEEEEEEECCCCCCCCCEEEEEECHHHHHHHHHHHHHHHCCCEEEEEEC
SSGETFAQLAAEAEQPRGDVWWGGTGDPHLQAAQEGLTEEYQSEMLGNLQDWAVRQAETA
CCCHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCC
NYRTVGIYAGGLGFGYNSDLVDGDAPACWADLLDERFADDVQVANPNSSGTSYTMLATMV
CEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHH
QLMGEDEAFAYLAELHNNISQYTQSGSAPIRAAATGETAVGIVFMHDAVAQTVQGAPIVA
HHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEEEHHHHHHHHCCCCEEE
VAPCEGTGYEVGSMSIIAGGPNPDSARTFYDWALTPEAQEIGAANNSFQIPSNLDSATPE
EECCCCCCEEECCEEEEECCCCCCCCCEEEEEECCCCHHHHCCCCCCEECCCCCCCCCCC
ASPRLEDITLIDYDFALYGSSEERTRLLQRWDADIGSLAQ
CCCCCCEEEEEEEEEEEECCCHHHHHHHHHHCCCHHHCCC
>Mature Secondary Structure
MRTTNLTAILVATTSLYVTAPTAAQADELVLYCSVQEEWCRAMSEAFERETGIEVLMTRR
CCCCCEEEEEEEEEEEEEECCCCCCCCCEEEEEECHHHHHHHHHHHHHHHCCCEEEEEEC
SSGETFAQLAAEAEQPRGDVWWGGTGDPHLQAAQEGLTEEYQSEMLGNLQDWAVRQAETA
CCCHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCC
NYRTVGIYAGGLGFGYNSDLVDGDAPACWADLLDERFADDVQVANPNSSGTSYTMLATMV
CEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHH
QLMGEDEAFAYLAELHNNISQYTQSGSAPIRAAATGETAVGIVFMHDAVAQTVQGAPIVA
HHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEEEHHHHHHHHCCCCEEE
VAPCEGTGYEVGSMSIIAGGPNPDSARTFYDWALTPEAQEIGAANNSFQIPSNLDSATPE
EECCCCCCEEECCEEEEECCCCCCCCCEEEEEECCCCHHHHCCCCCCEECCCCCCCCCCC
ASPRLEDITLIDYDFALYGSSEERTRLLQRWDADIGSLAQ
CCCCCCEEEEEEEEEEEECCCHHHHHHHHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]