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The map label for this gene is xylX [H]

Identifier: 89053834

GI number: 89053834

Start: 1308767

End: 1310077

Strand: Direct

Name: xylX [H]

Synonym: Jann_1343

Alternate gene names: 89053834

Gene position: 1308767-1310077 (Clockwise)

Preceding gene: 89053833

Following gene: 89053835

Centisome position: 30.31

GC content: 58.05

Gene sequence:

>1311_bases
ATGACACCCGATCAAATCCGGGGCCTCGTGCAAGGCCACCAGGTCCACCGCGATGTCTACACCAGCCAGGACGTCTACCG
CGCAGAGATGCGGCACCTGTTCGCCAACACCTGGGTCTTTGTCGGCCACGACAGTCAGACGCCGGGCAAGGGCGACTATA
TCACCACGCAGATCGGCGATCAGCCGGTTATTCAAGTGCGCCATTCCGATGGAGAGATCAAAGTCCTCCACAATCGCTGC
CCCCACAAAGGCACAAAGATTGCGATTGATCGACAGGGCAACACCGGTAAGTTCTTTCGATGCCCCTACCATGCCTGGAG
CTTCAAGACAGATGGCTGCCCTTTGGCGATCCCGCTGAAGAAGGGGTATGAAGGCACCGGCCTGCAACAGACGGAAAATG
CGGGCGGCATCAAGCCTGTGGGTGCGGTGCACGTGTATCGCGGCTTCGTCTTCGCGCGTCTGGCGTCTGAGGGGATCGGG
TTCGAGGCGTTTTTCGGCGATAGTCTCAGTTCTATCGACAACATGGTTGACCGCGCGCCAGAGGGGCGGCTGGAAGTCTC
AGGACCGCCGCTGCGCTACATGCATAAGTGCAACTGGAAAATGCTGGTCGAAAACCAGACCGATACCTGTCACCCGATGG
TGGCGCATGAAAGTTCGGCCGGGACTGCGATTGATATCTTCAAGTCCATGGGCGAGCCCGAGCCGCGCCCCGCTGCGATG
CAGATCATAGCCCCCTTCATGTCGGCCTATGAGTTCTTTGAGGAGATGGGCATTCGCACCTGGCCCAACGGCCATGGGCA
CACCGGTGTCAACATGTCGATCCACTCCAACTATGATGCCTTTCCCGGATATTTTGACGCGATGACAAAGGCCTACGGGG
AGGATCGCGCCAAGGCTATTCTGGATGAGAACCGCCATAACACCGTTTATTTCCCGAATGTGATGGTCAAAGCGCCATTG
GCACAATTGCGCAACTTCATCCCCATGGGCCCGAACAAGACGCTGGTGGAAAGCTATGTCTATCGCCCCGTGGGTGCGCC
CGATGAGATGACCGCCATGTCGGCAATGTATAACCGGATGATCAATGCGCCGACCTCCATCGTCGGGCACGATGACCTGG
AGATGTACGAGCGTGCGCAGGAGGGTCTGATGTCCAACGGGCTGGAGTGGGTGAACCTGCAACGCCTATACGAGGAAGAT
GAAGATTTCAGCGAAGAGGCGGTTGAGAACGGCACGACCGAGCGGCAAATGCGCAATCAGTTCAACGCCTGGGTCAAGTT
CATGACCGTGGACGCGGAGGCTGCGCCATGA

Upstream 100 bases:

>100_bases
GCGATGCGGAAAAGCAAAGCGGCAAGGTCATGCAAATCTGTGTCAGCCGGGCGCTGTCCAAGCGCCTTGTTCTGGATATC
TGAGGGGGGAGAGAAGAGTG

Downstream 100 bases:

>100_bases
CCATCACCCGGGATGACGTTGTCGACTTCATCTACGCCGAGGCGCGGATGCTGGACGAGGGGCGCTTTACCGAATGGTTG
GGGCTTTGGCTGCCCGATGC

Product: Rieske (2Fe-2S) protein

Products: NAD; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol [C]

Alternate protein names: NA

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MTPDQIRGLVQGHQVHRDVYTSQDVYRAEMRHLFANTWVFVGHDSQTPGKGDYITTQIGDQPVIQVRHSDGEIKVLHNRC
PHKGTKIAIDRQGNTGKFFRCPYHAWSFKTDGCPLAIPLKKGYEGTGLQQTENAGGIKPVGAVHVYRGFVFARLASEGIG
FEAFFGDSLSSIDNMVDRAPEGRLEVSGPPLRYMHKCNWKMLVENQTDTCHPMVAHESSAGTAIDIFKSMGEPEPRPAAM
QIIAPFMSAYEFFEEMGIRTWPNGHGHTGVNMSIHSNYDAFPGYFDAMTKAYGEDRAKAILDENRHNTVYFPNVMVKAPL
AQLRNFIPMGPNKTLVESYVYRPVGAPDEMTAMSAMYNRMINAPTSIVGHDDLEMYERAQEGLMSNGLEWVNLQRLYEED
EDFSEEAVENGTTERQMRNQFNAWVKFMTVDAEAAP

Sequences:

>Translated_436_residues
MTPDQIRGLVQGHQVHRDVYTSQDVYRAEMRHLFANTWVFVGHDSQTPGKGDYITTQIGDQPVIQVRHSDGEIKVLHNRC
PHKGTKIAIDRQGNTGKFFRCPYHAWSFKTDGCPLAIPLKKGYEGTGLQQTENAGGIKPVGAVHVYRGFVFARLASEGIG
FEAFFGDSLSSIDNMVDRAPEGRLEVSGPPLRYMHKCNWKMLVENQTDTCHPMVAHESSAGTAIDIFKSMGEPEPRPAAM
QIIAPFMSAYEFFEEMGIRTWPNGHGHTGVNMSIHSNYDAFPGYFDAMTKAYGEDRAKAILDENRHNTVYFPNVMVKAPL
AQLRNFIPMGPNKTLVESYVYRPVGAPDEMTAMSAMYNRMINAPTSIVGHDDLEMYERAQEGLMSNGLEWVNLQRLYEED
EDFSEEAVENGTTERQMRNQFNAWVKFMTVDAEAAP
>Mature_435_residues
TPDQIRGLVQGHQVHRDVYTSQDVYRAEMRHLFANTWVFVGHDSQTPGKGDYITTQIGDQPVIQVRHSDGEIKVLHNRCP
HKGTKIAIDRQGNTGKFFRCPYHAWSFKTDGCPLAIPLKKGYEGTGLQQTENAGGIKPVGAVHVYRGFVFARLASEGIGF
EAFFGDSLSSIDNMVDRAPEGRLEVSGPPLRYMHKCNWKMLVENQTDTCHPMVAHESSAGTAIDIFKSMGEPEPRPAAMQ
IIAPFMSAYEFFEEMGIRTWPNGHGHTGVNMSIHSNYDAFPGYFDAMTKAYGEDRAKAILDENRHNTVYFPNVMVKAPLA
QLRNFIPMGPNKTLVESYVYRPVGAPDEMTAMSAMYNRMINAPTSIVGHDDLEMYERAQEGLMSNGLEWVNLQRLYEEDE
DFSEEAVENGTTERQMRNQFNAWVKFMTVDAEAAP

Specific function: Part Of The Multicomponent 3-Phenylpropionate Dioxygenase. Converts 3-Phenylpropionic Acid (Pp) Into Cis-3-(3- Carboxyethyl)-3,5-Cyclohexadiene-1,2-Diol (Pp-Dihydrodiol). [C]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Escherichia coli, GI1788888, Length=384, Percent_Identity=28.125, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI1788103, Length=198, Percent_Identity=27.7777777777778, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017639
- InterPro:   IPR017941
- InterPro:   IPR015881
- InterPro:   IPR015879
- InterPro:   IPR001663 [H]

Pfam domain/function: PF00355 Rieske; PF00848 Ring_hydroxyl_A [H]

EC number: 1.14.1.- [C]

Molecular weight: Translated: 49049; Mature: 48918

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPDQIRGLVQGHQVHRDVYTSQDVYRAEMRHLFANTWVFVGHDSQTPGKGDYITTQIGD
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEECCCCCCCCCCEEEEECCC
QPVIQVRHSDGEIKVLHNRCPHKGTKIAIDRQGNTGKFFRCPYHAWSFKTDGCPLAIPLK
CCEEEEEECCCCEEEEECCCCCCCCEEEEECCCCCCCEEECCCCCCCCCCCCCCEEEECC
KGYEGTGLQQTENAGGIKPVGAVHVYRGFVFARLASEGIGFEAFFGDSLSSIDNMVDRAP
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHCCC
EGRLEVSGPPLRYMHKCNWKMLVENQTDTCHPMVAHESSAGTAIDIFKSMGEPEPRPAAM
CCCEEECCCCHHHHHCCCCEEEEECCCCCCCCEEEECCCCCHHHHHHHHCCCCCCCHHHH
QIIAPFMSAYEFFEEMGIRTWPNGHGHTGVNMSIHSNYDAFPGYFDAMTKAYGEDRAKAI
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCHHHHHHH
LDENRHNTVYFPNVMVKAPLAQLRNFIPMGPNKTLVESYVYRPVGAPDEMTAMSAMYNRM
HCCCCCCEEEECCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHH
INAPTSIVGHDDLEMYERAQEGLMSNGLEWVNLQRLYEEDEDFSEEAVENGTTERQMRNQ
HCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHH
FNAWVKFMTVDAEAAP
HHHEEEEEEECCCCCC
>Mature Secondary Structure 
TPDQIRGLVQGHQVHRDVYTSQDVYRAEMRHLFANTWVFVGHDSQTPGKGDYITTQIGD
CHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEECCCCCCCCCCEEEEECCC
QPVIQVRHSDGEIKVLHNRCPHKGTKIAIDRQGNTGKFFRCPYHAWSFKTDGCPLAIPLK
CCEEEEEECCCCEEEEECCCCCCCCEEEEECCCCCCCEEECCCCCCCCCCCCCCEEEECC
KGYEGTGLQQTENAGGIKPVGAVHVYRGFVFARLASEGIGFEAFFGDSLSSIDNMVDRAP
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHCCC
EGRLEVSGPPLRYMHKCNWKMLVENQTDTCHPMVAHESSAGTAIDIFKSMGEPEPRPAAM
CCCEEECCCCHHHHHCCCCEEEEECCCCCCCCEEEECCCCCHHHHHHHHCCCCCCCHHHH
QIIAPFMSAYEFFEEMGIRTWPNGHGHTGVNMSIHSNYDAFPGYFDAMTKAYGEDRAKAI
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCHHHHHHH
LDENRHNTVYFPNVMVKAPLAQLRNFIPMGPNKTLVESYVYRPVGAPDEMTAMSAMYNRM
HCCCCCCEEEECCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHH
INAPTSIVGHDDLEMYERAQEGLMSNGLEWVNLQRLYEEDEDFSEEAVENGTTERQMRNQ
HCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHH
FNAWVKFMTVDAEAAP
HHHEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 2Fe-2S. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; Proton; O2; 3-phenylpropionate [C]

Specific reaction: 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD 3-phenylpropionate + NADH + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1740120; 1938949 [H]