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The map label for this gene is yeaX [H]

Identifier: 89053833

GI number: 89053833

Start: 1307781

End: 1308749

Strand: Direct

Name: yeaX [H]

Synonym: Jann_1342

Alternate gene names: 89053833

Gene position: 1307781-1308749 (Clockwise)

Preceding gene: 89053828

Following gene: 89053834

Centisome position: 30.29

GC content: 58.62

Gene sequence:

>969_bases
ATGAAAATTCATATTAATTTGATGGACAAGTCAATGATACCCTTCACAGTCACCGAGAAGACCGCTCTGACAAAGCGCAT
TTCCGCGTTCACATTGCAACCCGCAGATGGGGCGCGACCGTTGGGCTGGGCCGCGGGGGCCCATGTTGACGTGGATCTGG
GGGAGGTGGGGGCGCGGTCTTATTCCCTTATTTCTTGGCCCGGAGTGGATACGAATTGCTACCGGATCGCCGTGCAACGT
GAAGAAAACGGCACCGGGGGCAGCGCTGCGATGCATGCGCTTTCGGTTGGTGCGACGGTTCCGATCTCTGCCCCCAAAAA
CAGTTTCGCACTTGAGGATACAGACGATCCGGTCGCGCTCATTGCGGGCGGCATCGGCATCACGCCGTTGATCTCCATGG
CGACAGAATTGCTGCAAGCCGGACGCCTGTTTGTGCTGCATTATGCGGGCCGGTCCGCTGAGGTTGTAGCTTATGAGCCT
GAACTGGCGCAGATGTTGGGTGATAGGCTGATCAGCCATTTCGACGATACCACGCCGCTGGATTTGCCCGCGACACTGGC
CATCCTGACCGACCATCGCCTCTATGTTTGCGGCCCCCGTGGTATGATTGATGCGACGCGCATTGCCGCCGAGGCCGCCG
ATATCCCTGGGTCCCGTGTCCATTTGGAGCTGTTCGCCAGCCCGGACGCGGATGGTGACGACGCCGCGTTCGAGGTCGAG
ATATCATCTACCGGGAATGTCTACACCGTGCCCCCCAACGTCTCCATTATAGAGGCGCTGGAGGCGGAGGGCGTCGATCT
TATGTATGATTGCCAGCGTGGCGATTGCGGCATTTGCCAGGTGGATGTGCTGGATGGCACGCCCGATCACCGTGACGTGG
TGTTGAGCGATGCGGAAAAGCAAAGCGGCAAGGTCATGCAAATCTGTGTCAGCCGGGCGCTGTCCAAGCGCCTTGTTCTG
GATATCTGA

Upstream 100 bases:

>100_bases
AAGTACAACAGGTAGTCGTCTACGAAGCCTGCATCTGCGCTATCTTGGGGACCATCGGCCATGTCTGACGCCCTCACAGC
AAAGTCTTGACAGAATATAT

Downstream 100 bases:

>100_bases
GGGGGGAGAGAAGAGTGATGACACCCGATCAAATCCGGGGCCTCGTGCAAGGCCACCAGGTCCACCGCGATGTCTACACC
AGCCAGGACGTCTACCGCGC

Product: ferredoxin

Products: 3,4-dihydroxybenzoate; NAD+; H2O; formaldehyde

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MKIHINLMDKSMIPFTVTEKTALTKRISAFTLQPADGARPLGWAAGAHVDVDLGEVGARSYSLISWPGVDTNCYRIAVQR
EENGTGGSAAMHALSVGATVPISAPKNSFALEDTDDPVALIAGGIGITPLISMATELLQAGRLFVLHYAGRSAEVVAYEP
ELAQMLGDRLISHFDDTTPLDLPATLAILTDHRLYVCGPRGMIDATRIAAEAADIPGSRVHLELFASPDADGDDAAFEVE
ISSTGNVYTVPPNVSIIEALEAEGVDLMYDCQRGDCGICQVDVLDGTPDHRDVVLSDAEKQSGKVMQICVSRALSKRLVL
DI

Sequences:

>Translated_322_residues
MKIHINLMDKSMIPFTVTEKTALTKRISAFTLQPADGARPLGWAAGAHVDVDLGEVGARSYSLISWPGVDTNCYRIAVQR
EENGTGGSAAMHALSVGATVPISAPKNSFALEDTDDPVALIAGGIGITPLISMATELLQAGRLFVLHYAGRSAEVVAYEP
ELAQMLGDRLISHFDDTTPLDLPATLAILTDHRLYVCGPRGMIDATRIAAEAADIPGSRVHLELFASPDADGDDAAFEVE
ISSTGNVYTVPPNVSIIEALEAEGVDLMYDCQRGDCGICQVDVLDGTPDHRDVVLSDAEKQSGKVMQICVSRALSKRLVL
DI
>Mature_322_residues
MKIHINLMDKSMIPFTVTEKTALTKRISAFTLQPADGARPLGWAAGAHVDVDLGEVGARSYSLISWPGVDTNCYRIAVQR
EENGTGGSAAMHALSVGATVPISAPKNSFALEDTDDPVALIAGGIGITPLISMATELLQAGRLFVLHYAGRSAEVVAYEP
ELAQMLGDRLISHFDDTTPLDLPATLAILTDHRLYVCGPRGMIDATRIAAEAADIPGSRVHLELFASPDADGDDAAFEVE
ISSTGNVYTVPPNVSIIEALEAEGVDLMYDCQRGDCGICQVDVLDGTPDHRDVVLSDAEKQSGKVMQICVSRALSKRLVL
DI

Specific function: Unknown

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788104, Length=327, Percent_Identity=38.5321100917431, Blast_Score=194, Evalue=6e-51,
Organism=Escherichia coli, GI1788903, Length=238, Percent_Identity=29.8319327731092, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1787658, Length=298, Percent_Identity=27.5167785234899, Blast_Score=75, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR000951
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.14.13.82

Molecular weight: Translated: 34271; Mature: 34271

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIHINLMDKSMIPFTVTEKTALTKRISAFTLQPADGARPLGWAAGAHVDVDLGEVGARS
CEEEEEEECCCCCCEEEECHHHHHHHHHEEEECCCCCCCCCCCCCCCEEEEEHHHCCCCC
YSLISWPGVDTNCYRIAVQREENGTGGSAAMHALSVGATVPISAPKNSFALEDTDDPVAL
EEEEECCCCCCCEEEEEEEECCCCCCHHHHHHHHHCCCEEECCCCCCCEECCCCCCCEEE
IAGGIGITPLISMATELLQAGRLFVLHYAGRSAEVVAYEPELAQMLGDRLISHFDDTTPL
EECCCCHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEECHHHHHHHHHHHHHHCCCCCCC
DLPATLAILTDHRLYVCGPRGMIDATRIAAEAADIPGSRVHLELFASPDADGDDAAFEVE
CCCEEEEEEECCEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCEEEEE
ISSTGNVYTVPPNVSIIEALEAEGVDLMYDCQRGDCGICQVDVLDGTPDHRDVVLSDAEK
ECCCCCEEECCCCCCEEEHHHCCCCEEEEECCCCCCCEEEEEEECCCCCCCEEEEECCCC
QSGKVMQICVSRALSKRLVLDI
CCCHHHHHHHHHHHHHHEEECC
>Mature Secondary Structure
MKIHINLMDKSMIPFTVTEKTALTKRISAFTLQPADGARPLGWAAGAHVDVDLGEVGARS
CEEEEEEECCCCCCEEEECHHHHHHHHHEEEECCCCCCCCCCCCCCCEEEEEHHHCCCCC
YSLISWPGVDTNCYRIAVQREENGTGGSAAMHALSVGATVPISAPKNSFALEDTDDPVAL
EEEEECCCCCCCEEEEEEEECCCCCCHHHHHHHHHCCCEEECCCCCCCEECCCCCCCEEE
IAGGIGITPLISMATELLQAGRLFVLHYAGRSAEVVAYEPELAQMLGDRLISHFDDTTPL
EECCCCHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEECHHHHHHHHHHHHHHCCCCCCC
DLPATLAILTDHRLYVCGPRGMIDATRIAAEAADIPGSRVHLELFASPDADGDDAAFEVE
CCCEEEEEEECCEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCEEEEE
ISSTGNVYTVPPNVSIIEALEAEGVDLMYDCQRGDCGICQVDVLDGTPDHRDVVLSDAEK
ECCCCCEEECCCCCCEEEHHHCCCCEEEEECCCCCCCEEEEEEECCCCCCCEEEEECCCC
QSGKVMQICVSRALSKRLVLDI
CCCHHHHHHHHHHHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: vanillate; O2; NADH; H+

Specific reaction: vanillate + O2 + NADH + H+ = 3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]