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The map label for this gene is bclA [H]

Identifier: 89053819

GI number: 89053819

Start: 1292670

End: 1294289

Strand: Direct

Name: bclA [H]

Synonym: Jann_1328

Alternate gene names: 89053819

Gene position: 1292670-1294289 (Clockwise)

Preceding gene: 89053818

Following gene: 89053820

Centisome position: 29.94

GC content: 62.22

Gene sequence:

>1620_bases
ATGCTAAGTGCCAGCGCCCATTCTGATACCTTCGCCCGCGATAACCTGCCCGCGCAGGACACGTGGCCAGAGTTTCTGCT
GGACGGCTTCGCCTATCCAGATCGCCTGAATGTGGGTGCCGAGCTGACGGACGCGATGGTCGCGCAGGGCTTCGGCGATC
ATACGGCGCTGATCGGCAATGGCCGCCGCCGCACGTATAAGGAGCTGACGGATTGGACGAACCGCATCGCCCATGTGCTG
GTCGATGATATGGGCGTGCAGCCGGGTAATCGCGTCCTGATCCGGTCGGCCAACAATCCGGCGATGGTCGCCTGCTGGCT
GGCCGCCACGAAGGTGGGCGCGGTTGTCGTGAACTCCATGCCGATGCTGCGCGCCGGAGAGATCAGCAAATATGTCGACA
AGGCGCAGATTGCGTTTGCGCTGTGCGACACCCGCCTGATGGAAGAGATGGAGGCCTGCGCTGCGTCCAATCCGACGCTG
CAACGGATCATGGGCTTCGATGGCACCTCCAACCACGATGCGGAACTGGACCGGTTGGCGTTGGAGAAACCGGTGCAATT
CACCGCTGTGGCCACCGCCGCCGATGATGTGGCGCTGCTTGGCTTTACCTCAGGCTCCACTGGCGATCCCAAGGCCACCA
TGCACTTCCACCGCGATCTTCTGATCATCGCAGACGGTTACGCGAAGGAGGTTCTGGACGTCACACCGCAGGATGTCTTC
ATCGGCTCACCACCCCTGGCCTTCACCTTCGGCCTCGGCGGCCTGGCGATTTTCCCGCTGCGGTTCGGGGCGGCCGCGAC
CCTGCTGGAGGTTGCCACGCCGCCCCTGATGGTCGAGATCATCGAGAAGTATAAAGCCACCGTCTGCTTCACCGCGCCCA
CGGCCTATCGCGCCATGTTGCGCGCGATGGATGAGGGGGCGGACCTGTCTTCGCTGCGCGCGGCTGTCTCTGCGGGAGAG
ACCCTGCCTGCGCCTGTCTATGACGATTGGATCGCCAAGACCGGTAAACCGATGCTTGACGGGATCGGCGCGACGGAGAT
GCTGCACATCTTCATCTCCAACCGGTTCGATGATCACCGACCCGCCTGCACGGGCAAACCTGTCAGCGGTTATCTTGCCA
AGATCATCGGCGAAGATGGGACGGAGGTGCCGCGCGGAACGCCGGGCCGTCTGGCGCTGAAAGGGCCCACCGGATGTCGC
TACCTTGGGGGTCAACGTCAGGAAGATTACGTCCAGGACGGCTGGAACATCTCGGGCGATACGTTTGTGCAGGATGAGGA
CGGCTATTTCCACTTCGCGGCCCGCAACGACGACATGATCATCTCCTCGGGCTACAACATCGCGGGACCTGAAGTTGAAG
CAGCGTTGCTATCGCATCCGGACGTCAACGAATGTGCTGTAATCGGCGCCCCGGATGAGGAGCGCGGATCGGTGGTTGAG
GCGCACATCGTGGTGACCGAGGGCGTGGCCGAGACGGCGACGCAGGCCAAGGTCTTGCAAGATCATGTGAAAGCGACGAT
CGCACCGTTCAAATATCCACGCAGCGTCGTGTTCGTCCGCGACCTTCCGAAAACCGCGACCGGAAAGATCCAACGGTTCC
TGTTGAAGCCCGGTGGGTGA

Upstream 100 bases:

>100_bases
TGAGAAACTCTACCGTGAAATCAGGGCGTTGCGCATCTATGAGGGTGCCTCGGACGTCCAGCGTGTGATCATCGCGCGCC
AGACGATCGGAGCGTAACCC

Downstream 100 bases:

>100_bases
CACAGGCTCGCCACTGAACCCCGGTGCGATCGTGCCCGCATCAAACTTCTGGAGGCGCATCGCTGACGCTCCGCAAGACC
GAACTGAGACATCACGGATG

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Benzoyl-CoA synthetase [H]

Number of amino acids: Translated: 539; Mature: 539

Protein sequence:

>539_residues
MLSASAHSDTFARDNLPAQDTWPEFLLDGFAYPDRLNVGAELTDAMVAQGFGDHTALIGNGRRRTYKELTDWTNRIAHVL
VDDMGVQPGNRVLIRSANNPAMVACWLAATKVGAVVVNSMPMLRAGEISKYVDKAQIAFALCDTRLMEEMEACAASNPTL
QRIMGFDGTSNHDAELDRLALEKPVQFTAVATAADDVALLGFTSGSTGDPKATMHFHRDLLIIADGYAKEVLDVTPQDVF
IGSPPLAFTFGLGGLAIFPLRFGAAATLLEVATPPLMVEIIEKYKATVCFTAPTAYRAMLRAMDEGADLSSLRAAVSAGE
TLPAPVYDDWIAKTGKPMLDGIGATEMLHIFISNRFDDHRPACTGKPVSGYLAKIIGEDGTEVPRGTPGRLALKGPTGCR
YLGGQRQEDYVQDGWNISGDTFVQDEDGYFHFAARNDDMIISSGYNIAGPEVEAALLSHPDVNECAVIGAPDEERGSVVE
AHIVVTEGVAETATQAKVLQDHVKATIAPFKYPRSVVFVRDLPKTATGKIQRFLLKPGG

Sequences:

>Translated_539_residues
MLSASAHSDTFARDNLPAQDTWPEFLLDGFAYPDRLNVGAELTDAMVAQGFGDHTALIGNGRRRTYKELTDWTNRIAHVL
VDDMGVQPGNRVLIRSANNPAMVACWLAATKVGAVVVNSMPMLRAGEISKYVDKAQIAFALCDTRLMEEMEACAASNPTL
QRIMGFDGTSNHDAELDRLALEKPVQFTAVATAADDVALLGFTSGSTGDPKATMHFHRDLLIIADGYAKEVLDVTPQDVF
IGSPPLAFTFGLGGLAIFPLRFGAAATLLEVATPPLMVEIIEKYKATVCFTAPTAYRAMLRAMDEGADLSSLRAAVSAGE
TLPAPVYDDWIAKTGKPMLDGIGATEMLHIFISNRFDDHRPACTGKPVSGYLAKIIGEDGTEVPRGTPGRLALKGPTGCR
YLGGQRQEDYVQDGWNISGDTFVQDEDGYFHFAARNDDMIISSGYNIAGPEVEAALLSHPDVNECAVIGAPDEERGSVVE
AHIVVTEGVAETATQAKVLQDHVKATIAPFKYPRSVVFVRDLPKTATGKIQRFLLKPGG
>Mature_539_residues
MLSASAHSDTFARDNLPAQDTWPEFLLDGFAYPDRLNVGAELTDAMVAQGFGDHTALIGNGRRRTYKELTDWTNRIAHVL
VDDMGVQPGNRVLIRSANNPAMVACWLAATKVGAVVVNSMPMLRAGEISKYVDKAQIAFALCDTRLMEEMEACAASNPTL
QRIMGFDGTSNHDAELDRLALEKPVQFTAVATAADDVALLGFTSGSTGDPKATMHFHRDLLIIADGYAKEVLDVTPQDVF
IGSPPLAFTFGLGGLAIFPLRFGAAATLLEVATPPLMVEIIEKYKATVCFTAPTAYRAMLRAMDEGADLSSLRAAVSAGE
TLPAPVYDDWIAKTGKPMLDGIGATEMLHIFISNRFDDHRPACTGKPVSGYLAKIIGEDGTEVPRGTPGRLALKGPTGCR
YLGGQRQEDYVQDGWNISGDTFVQDEDGYFHFAARNDDMIISSGYNIAGPEVEAALLSHPDVNECAVIGAPDEERGSVVE
AHIVVTEGVAETATQAKVLQDHVKATIAPFKYPRSVVFVRDLPKTATGKIQRFLLKPGG

Specific function: Catalyzes the ligation of benzoate and CoA to form benzoyl-CoA at the expense of ATP. The enzyme also ligates 2- aminobenzoate and CoA. The enzyme shows activity toward a number of benzoate derivates [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. Benzoate-CoA ligase subfamily [H]

Homologues:

Organism=Homo sapiens, GI42544132, Length=490, Percent_Identity=29.5918367346939, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI122937307, Length=500, Percent_Identity=29.6, Blast_Score=198, Evalue=9e-51,
Organism=Homo sapiens, GI38505220, Length=487, Percent_Identity=28.7474332648871, Blast_Score=193, Evalue=4e-49,
Organism=Homo sapiens, GI58082049, Length=543, Percent_Identity=27.8084714548803, Blast_Score=188, Evalue=9e-48,
Organism=Homo sapiens, GI157311624, Length=543, Percent_Identity=27.0718232044199, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI157311622, Length=543, Percent_Identity=27.0718232044199, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI115511026, Length=494, Percent_Identity=27.9352226720648, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI28416953, Length=554, Percent_Identity=28.5198555956679, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI187761345, Length=532, Percent_Identity=25.5639097744361, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI187761343, Length=532, Percent_Identity=25.5639097744361, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI156151445, Length=518, Percent_Identity=27.027027027027, Blast_Score=129, Evalue=8e-30,
Organism=Homo sapiens, GI13375727, Length=562, Percent_Identity=25.2669039145907, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI8923896, Length=374, Percent_Identity=28.8770053475936, Blast_Score=120, Evalue=4e-27,
Organism=Homo sapiens, GI253970489, Length=374, Percent_Identity=28.8770053475936, Blast_Score=120, Evalue=4e-27,
Organism=Homo sapiens, GI195947378, Length=453, Percent_Identity=23.1788079470199, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI42544134, Length=320, Percent_Identity=24.0625, Blast_Score=96, Evalue=7e-20,
Organism=Escherichia coli, GI145693145, Length=508, Percent_Identity=28.740157480315, Blast_Score=175, Evalue=6e-45,
Organism=Escherichia coli, GI1790505, Length=544, Percent_Identity=27.7573529411765, Blast_Score=165, Evalue=6e-42,
Organism=Escherichia coli, GI1788107, Length=542, Percent_Identity=26.0147601476015, Blast_Score=163, Evalue=3e-41,
Organism=Escherichia coli, GI221142682, Length=511, Percent_Identity=25.8317025440313, Blast_Score=140, Evalue=3e-34,
Organism=Escherichia coli, GI1786810, Length=521, Percent_Identity=26.1036468330134, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1786529, Length=525, Percent_Identity=26.2857142857143, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1789201, Length=358, Percent_Identity=26.536312849162, Blast_Score=85, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17538037, Length=505, Percent_Identity=25.7425742574257, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI32563687, Length=485, Percent_Identity=25.1546391752577, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17558820, Length=489, Percent_Identity=24.9488752556237, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17557194, Length=502, Percent_Identity=25.6972111553785, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI71994694, Length=538, Percent_Identity=26.3940520446097, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71994703, Length=538, Percent_Identity=26.3940520446097, Blast_Score=120, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI71994690, Length=538, Percent_Identity=26.3940520446097, Blast_Score=120, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI71996755, Length=492, Percent_Identity=24.7967479674797, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17559526, Length=368, Percent_Identity=28.2608695652174, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17531443, Length=497, Percent_Identity=23.7424547283702, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71982997, Length=574, Percent_Identity=23.5191637630662, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI71983001, Length=574, Percent_Identity=23.5191637630662, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17560308, Length=523, Percent_Identity=24.8565965583174, Blast_Score=96, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17560140, Length=501, Percent_Identity=23.5528942115768, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71985884, Length=382, Percent_Identity=27.2251308900524, Blast_Score=93, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6319264, Length=528, Percent_Identity=28.4090909090909, Blast_Score=155, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6323182, Length=523, Percent_Identity=28.6806883365201, Blast_Score=146, Evalue=8e-36,
Organism=Saccharomyces cerevisiae, GI6319699, Length=359, Percent_Identity=28.9693593314763, Blast_Score=123, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24667955, Length=561, Percent_Identity=27.9857397504456, Blast_Score=139, Evalue=6e-33,
Organism=Drosophila melanogaster, GI62472339, Length=529, Percent_Identity=27.9773156899811, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI21355225, Length=564, Percent_Identity=25.531914893617, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24581924, Length=540, Percent_Identity=26.1111111111111, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI21355181, Length=509, Percent_Identity=28.0943025540275, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24667959, Length=364, Percent_Identity=29.9450549450549, Blast_Score=123, Evalue=4e-28,
Organism=Drosophila melanogaster, GI18859661, Length=358, Percent_Identity=27.6536312849162, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24653035, Length=487, Percent_Identity=23.6139630390144, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI20130357, Length=495, Percent_Identity=25.8585858585859, Blast_Score=86, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24648260, Length=492, Percent_Identity=24.1869918699187, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI21356441, Length=364, Percent_Identity=25.5494505494505, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI161076582, Length=502, Percent_Identity=22.7091633466135, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648253, Length=366, Percent_Identity=25.4098360655738, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24648255, Length=366, Percent_Identity=25.4098360655738, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI19922652, Length=496, Percent_Identity=22.9838709677419, Blast_Score=73, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24648257, Length=364, Percent_Identity=25.8241758241758, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI21356947, Length=496, Percent_Identity=23.5887096774194, Blast_Score=69, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR011957 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.25 [H]

Molecular weight: Translated: 57993; Mature: 57993

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSASAHSDTFARDNLPAQDTWPEFLLDGFAYPDRLNVGAELTDAMVAQGFGDHTALIGN
CCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEC
GRRRTYKELTDWTNRIAHVLVDDMGVQPGNRVLIRSANNPAMVACWLAATKVGAVVVNSM
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEHHHHHHHHHEECCC
PMLRAGEISKYVDKAQIAFALCDTRLMEEMEACAASNPTLQRIMGFDGTSNHDAELDRLA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCHHHHHHH
LEKPVQFTAVATAADDVALLGFTSGSTGDPKATMHFHRDLLIIADGYAKEVLDVTPQDVF
HHCCCEEEEEEECCCCEEEEEECCCCCCCCCEEEEECCEEEEEECCHHHHHHHCCCCCEE
IGSPPLAFTFGLGGLAIFPLRFGAAATLLEVATPPLMVEIIEKYKATVCFTAPTAYRAML
ECCCCCEEEECCCCCEEEHHHHHHHHHHHHHCCCHHHHHHHHHHCCEEEEECCHHHHHHH
RAMDEGADLSSLRAAVSAGETLPAPVYDDWIAKTGKPMLDGIGATEMLHIFISNRFDDHR
HHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHCCCHHHHCCCHHHHHHHHHHCCCCCCC
PACTGKPVSGYLAKIIGEDGTEVPRGTPGRLALKGPTGCRYLGGQRQEDYVQDGWNISGD
CCCCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHCCCCCCCC
TFVQDEDGYFHFAARNDDMIISSGYNIAGPEVEAALLSHPDVNECAVIGAPDEERGSVVE
EEEECCCCEEEEEECCCCEEEECCCCCCCCCHHHHHHCCCCCCCEEEECCCCCCCCCEEE
AHIVVTEGVAETATQAKVLQDHVKATIAPFKYPRSVVFVRDLPKTATGKIQRFLLKPGG
EEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHEECCCC
>Mature Secondary Structure
MLSASAHSDTFARDNLPAQDTWPEFLLDGFAYPDRLNVGAELTDAMVAQGFGDHTALIGN
CCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEC
GRRRTYKELTDWTNRIAHVLVDDMGVQPGNRVLIRSANNPAMVACWLAATKVGAVVVNSM
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEHHHHHHHHHEECCC
PMLRAGEISKYVDKAQIAFALCDTRLMEEMEACAASNPTLQRIMGFDGTSNHDAELDRLA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCHHHHHHH
LEKPVQFTAVATAADDVALLGFTSGSTGDPKATMHFHRDLLIIADGYAKEVLDVTPQDVF
HHCCCEEEEEEECCCCEEEEEECCCCCCCCCEEEEECCEEEEEECCHHHHHHHCCCCCEE
IGSPPLAFTFGLGGLAIFPLRFGAAATLLEVATPPLMVEIIEKYKATVCFTAPTAYRAML
ECCCCCEEEECCCCCEEEHHHHHHHHHHHHHCCCHHHHHHHHHHCCEEEEECCHHHHHHH
RAMDEGADLSSLRAAVSAGETLPAPVYDDWIAKTGKPMLDGIGATEMLHIFISNRFDDHR
HHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHCCCHHHHCCCHHHHHHHHHHCCCCCCC
PACTGKPVSGYLAKIIGEDGTEVPRGTPGRLALKGPTGCRYLGGQRQEDYVQDGWNISGD
CCCCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHCCCCCCCC
TFVQDEDGYFHFAARNDDMIISSGYNIAGPEVEAALLSHPDVNECAVIGAPDEERGSVVE
EEEECCCCEEEEEECCCCEEEECCCCCCCCCHHHHHHCCCCCCCEEEECCCCCCCCCEEE
AHIVVTEGVAETATQAKVLQDHVKATIAPFKYPRSVVFVRDLPKTATGKIQRFLLKPGG
EEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12897012 [H]