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The map label for this gene is nuoB [H]

Identifier: 89053661

GI number: 89053661

Start: 1134973

End: 1135506

Strand: Direct

Name: nuoB [H]

Synonym: Jann_1170

Alternate gene names: 89053661

Gene position: 1134973-1135506 (Clockwise)

Preceding gene: 89053660

Following gene: 89053662

Centisome position: 26.28

GC content: 62.17

Gene sequence:

>534_bases
ATGTCCGTCGCAACCAATCCCAACCACGCGGGCGCAGACCGGGAGGTGGGGACGCAAGCCCTCAACCGGGAGCTTCAGGA
CAAGGGGTTTCTTGTCACTTCGTCCGAGGACATCATCAATTGGGCGCGCACCGGATCGCTGCACTGGATGACGTTTGGTC
TGGCGTGTTGCGCGGTGGAGATGATGCACACGTCGATGCCGCGCTATGATGCGGAGCGGTTCGGGATCGCGCCGCGCGCT
TCCCCCCGCCAGTCGGATGTGATGATTGTGGCGGGCACGCTGACCAACAAGATGGCACCCGCGCTGCGCAAGGTCTACGA
CCAGATGCCAGAGCCGCGCTACGTGATCTCCATGGGGTCGTGTGCCAATGGCGGCGGGTATTATCACTACAGCTATAGCG
TTGTGCGCGGCTGTGATCGGATCGTGCCCGTTGACATTTATGTGCCCGGCTGCCCCCCCACGGCGGAGGCGCTTTTGTAT
GGTATCCTCCAGTTGCAGCGGAAGATCCGCCGCACCGGCACGATTGCAAGGTAA

Upstream 100 bases:

>100_bases
TCTGGGTGATCCCGGTGAGGACACGCGTTCGCTTTGTCGCGTTGACCGCCGTCGTCTGCGCCCGTATTTGATGCGCAAGC
TAGCAGTGAGGTGAGCCATC

Downstream 100 bases:

>100_bases
GGCGAGATCATGAGTGATGCCCTGAAAGAACTGGCCGAATATCTGTCCGAGAAGCGCTCGGACGATGTGGTGTCCACCGA
TGTTAACGCGATGGGAGAGT

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B [H]

Number of amino acids: Translated: 177; Mature: 176

Protein sequence:

>177_residues
MSVATNPNHAGADREVGTQALNRELQDKGFLVTSSEDIINWARTGSLHWMTFGLACCAVEMMHTSMPRYDAERFGIAPRA
SPRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLY
GILQLQRKIRRTGTIAR

Sequences:

>Translated_177_residues
MSVATNPNHAGADREVGTQALNRELQDKGFLVTSSEDIINWARTGSLHWMTFGLACCAVEMMHTSMPRYDAERFGIAPRA
SPRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLY
GILQLQRKIRRTGTIAR
>Mature_176_residues
SVATNPNHAGADREVGTQALNRELQDKGFLVTSSEDIINWARTGSLHWMTFGLACCAVEMMHTSMPRYDAERFGIAPRAS
PRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYG
ILQLQRKIRRTGTIAR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=142, Percent_Identity=83.8028169014084, Blast_Score=258, Evalue=2e-69,
Organism=Escherichia coli, GI1788624, Length=165, Percent_Identity=46.0606060606061, Blast_Score=156, Evalue=6e-40,
Organism=Escherichia coli, GI1789074, Length=126, Percent_Identity=45.2380952380952, Blast_Score=111, Evalue=3e-26,
Organism=Escherichia coli, GI1788834, Length=122, Percent_Identity=42.6229508196721, Blast_Score=102, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17509685, Length=170, Percent_Identity=62.3529411764706, Blast_Score=233, Evalue=3e-62,
Organism=Drosophila melanogaster, GI18859983, Length=140, Percent_Identity=78.5714285714286, Blast_Score=251, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24642371, Length=140, Percent_Identity=78.5714285714286, Blast_Score=251, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24651058, Length=162, Percent_Identity=70.9876543209877, Blast_Score=247, Evalue=2e-66,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 19547; Mature: 19416

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
7.9 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
7.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVATNPNHAGADREVGTQALNRELQDKGFLVTSSEDIINWARTGSLHWMTFGLACCAVE
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEECCHHHHHHHHCCCCHHHHHHHHHHHHH
MMHTSMPRYDAERFGIAPRASPRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGS
HHHHCCCCCCHHHHCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHCCCCEEEEEECC
CANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGILQLQRKIRRTGTIAR
CCCCCCEEEEEHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SVATNPNHAGADREVGTQALNRELQDKGFLVTSSEDIINWARTGSLHWMTFGLACCAVE
CCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEECCHHHHHHHHCCCCHHHHHHHHHHHHH
MMHTSMPRYDAERFGIAPRASPRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGS
HHHHCCCCCCHHHHCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHCCCCEEEEEECC
CANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGILQLQRKIRRTGTIAR
CCCCCCEEEEEHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA