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The map label for this gene is menB [C]

Identifier: 89053641

GI number: 89053641

Start: 1111848

End: 1112630

Strand: Direct

Name: menB [C]

Synonym: Jann_1150

Alternate gene names: 89053641

Gene position: 1111848-1112630 (Clockwise)

Preceding gene: 89053640

Following gene: 89053642

Centisome position: 25.75

GC content: 69.99

Gene sequence:

>783_bases
ATGGACACTTTGGATGTAACGGTGGACGCGCGCGGGGTGGCCACTCTGACGCTGAACCGGGTGGACGTGCACAACGCCAT
GAACGCCGCGATGATCGCGGAGCTGGCAAATGCGGCCCAACGTTTGGGTCGTGACCCGGCGGTGCGGGTCGTGGTGCTGA
CCGGGGCGGGCAAAAGCTTCTGCGCGGGCGGTGATCTGGCCTGGATGCGCGCGCAGATGGCGGCGGACGCGGAGACGCGC
GCGAGGGAGGCCCGCGGTCTGGCCGAGATGTTGGGGGCGCTCGACCGTCTGCCCAAGCCGCTGATCGGGCGCATTCAGGG
GCAGGCCTTCGGCGGTGGCGTTGGCCTGATGTCGGTCTGCGATGTGGCGGTGGGCGTCAACGGTGCAACATTCGGCCTGA
CGGAGGTGCGCCTTGGTCTGATCCCGGCCACCATCGGGCCCTATGTCGTGGCGCGGATGGGGGCTGCGCGGGCGCGGCGG
GTGTTCTTCTCGGGCCGCCGCTTCGGCGCGGAGGAGGCGGTGGCGCTTGGCCTGTTGGCCCGCACCGTGCCTGAGGCGGA
TCTGGATGCCGCCGTCGAGGCAGAGGTGACGCCGTATCTTGCCGCGGCCCCCGGAGCCGTGGCCGACAGCAAGGCGCTGG
TAGCGCGGCTTGGCGGCGGCGTCTCGGACGCGGATATCACCGCCACGATTGACGCCCTTGTGGGCCGTTGGGAAAGCGCG
GAAGCCGCCGACGGGATCGCGGCATTCTTCGACAAGCGCAAACCCGGATGGGTCGAGACCTAG

Upstream 100 bases:

>100_bases
TGGACGCGCATCTGCGCCGACGCGGGTTCGAGACGGGTCTGGATGCCGATGTGCTGGCCCGGGCCGGGGCTTTGGCGCGC
GGTTTGCGGAAGGAATGACG

Downstream 100 bases:

>100_bases
GCGGCAACAGGACCCCGCGCCCCGACACTAACGCCCCATCCCTCCGCGCCACGCTCATGGTGCTGCGCTGCGGCGTGGAA
CGCGCTGGACAGACGGCGCG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MDTLDVTVDARGVATLTLNRVDVHNAMNAAMIAELANAAQRLGRDPAVRVVVLTGAGKSFCAGGDLAWMRAQMAADAETR
AREARGLAEMLGALDRLPKPLIGRIQGQAFGGGVGLMSVCDVAVGVNGATFGLTEVRLGLIPATIGPYVVARMGAARARR
VFFSGRRFGAEEAVALGLLARTVPEADLDAAVEAEVTPYLAAAPGAVADSKALVARLGGGVSDADITATIDALVGRWESA
EAADGIAAFFDKRKPGWVET

Sequences:

>Translated_260_residues
MDTLDVTVDARGVATLTLNRVDVHNAMNAAMIAELANAAQRLGRDPAVRVVVLTGAGKSFCAGGDLAWMRAQMAADAETR
AREARGLAEMLGALDRLPKPLIGRIQGQAFGGGVGLMSVCDVAVGVNGATFGLTEVRLGLIPATIGPYVVARMGAARARR
VFFSGRRFGAEEAVALGLLARTVPEADLDAAVEAEVTPYLAAAPGAVADSKALVARLGGGVSDADITATIDALVGRWESA
EAADGIAAFFDKRKPGWVET
>Mature_260_residues
MDTLDVTVDARGVATLTLNRVDVHNAMNAAMIAELANAAQRLGRDPAVRVVVLTGAGKSFCAGGDLAWMRAQMAADAETR
AREARGLAEMLGALDRLPKPLIGRIQGQAFGGGVGLMSVCDVAVGVNGATFGLTEVRLGLIPATIGPYVVARMGAARARR
VFFSGRRFGAEEAVALGLLARTVPEADLDAAVEAEVTPYLAAAPGAVADSKALVARLGGGVSDADITATIDALVGRWESA
EAADGIAAFFDKRKPGWVET

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI45643119, Length=255, Percent_Identity=27.4509803921569, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI260275230, Length=255, Percent_Identity=27.4509803921569, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI260274832, Length=255, Percent_Identity=27.4509803921569, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI70995211, Length=204, Percent_Identity=30.3921568627451, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI194097323, Length=253, Percent_Identity=30.0395256916996, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI31542718, Length=253, Percent_Identity=24.901185770751, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI116235487, Length=232, Percent_Identity=22.4137931034483, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI221136756, Length=236, Percent_Identity=24.1525423728814, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI221136753, Length=236, Percent_Identity=24.1525423728814, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI221307494, Length=236, Percent_Identity=24.1525423728814, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI68989263, Length=169, Percent_Identity=30.1775147928994, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI25453481, Length=232, Percent_Identity=22.4137931034483, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI51538573, Length=232, Percent_Identity=22.4137931034483, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI4757966, Length=192, Percent_Identity=24.4791666666667, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI51538581, Length=192, Percent_Identity=24.4791666666667, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI4757968, Length=232, Percent_Identity=22.4137931034483, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI49355787, Length=232, Percent_Identity=22.4137931034483, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI37594471, Length=154, Percent_Identity=30.5194805194805, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI37594469, Length=154, Percent_Identity=30.5194805194805, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI4502327, Length=268, Percent_Identity=27.6119402985075, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1788597, Length=251, Percent_Identity=29.4820717131474, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1787659, Length=265, Percent_Identity=31.6981132075472, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1787660, Length=260, Percent_Identity=31.5384615384615, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI221142681, Length=262, Percent_Identity=29.7709923664122, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI87082183, Length=260, Percent_Identity=25, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1788682, Length=197, Percent_Identity=28.9340101522843, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17536985, Length=185, Percent_Identity=32.972972972973, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI25145438, Length=270, Percent_Identity=29.6296296296296, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17535521, Length=259, Percent_Identity=27.027027027027, Blast_Score=90, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17534483, Length=185, Percent_Identity=28.6486486486486, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17540714, Length=223, Percent_Identity=30.0448430493274, Blast_Score=87, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17554946, Length=258, Percent_Identity=27.1317829457364, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17549921, Length=213, Percent_Identity=26.7605633802817, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17560910, Length=179, Percent_Identity=31.2849162011173, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17558304, Length=164, Percent_Identity=28.6585365853659, Blast_Score=70, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI25144160, Length=202, Percent_Identity=29.7029702970297, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI25144157, Length=202, Percent_Identity=29.7029702970297, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17508953, Length=122, Percent_Identity=33.6065573770492, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17508951, Length=122, Percent_Identity=33.6065573770492, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI25144276, Length=122, Percent_Identity=33.6065573770492, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17540306, Length=258, Percent_Identity=25.5813953488372, Blast_Score=67, Evalue=7e-12,
Organism=Drosophila melanogaster, GI19922422, Length=251, Percent_Identity=26.6932270916335, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI20129971, Length=185, Percent_Identity=31.8918918918919, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24653477, Length=185, Percent_Identity=31.8918918918919, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24653139, Length=258, Percent_Identity=29.0697674418605, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19920382, Length=203, Percent_Identity=29.064039408867, Blast_Score=84, Evalue=8e-17,
Organism=Drosophila melanogaster, GI21357171, Length=179, Percent_Identity=26.8156424581006, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 26786; Mature: 26786

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTLDVTVDARGVATLTLNRVDVHNAMNAAMIAELANAAQRLGRDPAVRVVVLTGAGKSF
CCCEEEEEECCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCC
CAGGDLAWMRAQMAADAETRAREARGLAEMLGALDRLPKPLIGRIQGQAFGGGVGLMSVC
CCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHH
DVAVGVNGATFGLTEVRLGLIPATIGPYVVARMGAARARRVFFSGRRFGAEEAVALGLLA
HHHHCCCCCEECHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
RTVPEADLDAAVEAEVTPYLAAAPGAVADSKALVARLGGGVSDADITATIDALVGRWESA
HHCCCCCCCHHHHCCCCCCEECCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCC
EAADGIAAFFDKRKPGWVET
HHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MDTLDVTVDARGVATLTLNRVDVHNAMNAAMIAELANAAQRLGRDPAVRVVVLTGAGKSF
CCCEEEEEECCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCC
CAGGDLAWMRAQMAADAETRAREARGLAEMLGALDRLPKPLIGRIQGQAFGGGVGLMSVC
CCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHH
DVAVGVNGATFGLTEVRLGLIPATIGPYVVARMGAARARRVFFSGRRFGAEEAVALGLLA
HHHHCCCCCEECHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
RTVPEADLDAAVEAEVTPYLAAAPGAVADSKALVARLGGGVSDADITATIDALVGRWESA
HHCCCCCCCHHHHCCCCCCEECCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCC
EAADGIAAFFDKRKPGWVET
HHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA