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The map label for this gene is purR [H]

Identifier: 89053598

GI number: 89053598

Start: 1067701

End: 1068738

Strand: Direct

Name: purR [H]

Synonym: Jann_1107

Alternate gene names: 89053598

Gene position: 1067701-1068738 (Clockwise)

Preceding gene: 89053597

Following gene: 89053599

Centisome position: 24.73

GC content: 64.16

Gene sequence:

>1038_bases
ATGATTGATCGCGCGCCCATTACAGGGAAAGTCACCTCCATCGACGTGGCGCAGCGGGCAGGCGTCAGCCAATCGGCGGT
CAGCCGGGTCTTCACGCCGGGGGCCAGCGTGTCCAAGAAGATGGAGGCGCGGGTGCGCGAAGCCGCTGACGATCTGGGCT
ACCGGCCCAATGTGCTGGCGCGGTCGCTGATCACCGGACGCTCGCGGATTATCGGGCTGGTCGTGGCGCATCTGGACAAT
TCCTTCTACCCCGATGCGCTGGAAAAACTCAGCGCCGCGCTTCAGGCCAAGGGCTACCATATCCTTGTGTTCATGGCTGG
AAATTCGGGCGATGACGTCGACAGCGTGATCCACGATCTGATGGATTATCAGGTGGATGGGATCATCACGGCCTCCATCA
ACCTCAGCGGTGAATTGACCGACCGGTGCCGGGCTGCGGGCCTTCCGGTGCTGTTGTTCAACCGGGGGATCGAAGGCTCG
GGCCTCAGTTCGGTCACGTCGGCCAACCGGATCGGCGGGGCTGCGGTGGCCAATACGCTTCTGGACGCGGGCCATACAAA
AATTGCCACGATCTCGGGCTGGATGGGGGCGTCCACCGGGCGGGATCGCCGCGATGGTTTCGTGGCGGCGCTGGAGGCGC
GGGGCGCACAGGTCCATGCCGAGGCCGATGGCTTGTATGACCGCGACACAGCCGCCCGGGTCGCCCGCGACATGATGCAG
GCCGCACATCCCCCCGACGCGATTTTCGTGGGCAACGACCATATGGCCATGGCCGTCATGGACACGCTGCGCTTTGATCT
GGGCCTGAGCGTGCCCGATGACGTGTCCATCATCGGCTATGACGATGTGCCGATGGCGGCCTGGCCGTCCTACAACCTGA
CGACGCTGCGCCAGCCGGTGAACCGGATGGTGGAGGCGACAGTCGACACCATGCTTGATGAAATCGAAACCGGGCGCGCG
CCTGGCACGCGGGTCGAGATTGAGGGGGAGCTGATCCTCCGCACCTCTGCCCGCCTTCCCAAAGGACCAACCATATGA

Upstream 100 bases:

>100_bases
AGGTGGAGGATATCATGGGGGCGGTTCTGTTTCTGGCATCGGGTGCCAGCGCCATGGTGACGGGCACGTCGATCCTTGTC
GACGGGGGATGGACGGCGGG

Downstream 100 bases:

>100_bases
AGGGCTTCGACGCCAAATTCCGCGATTTCCCCGACTACATCGTCGGCATCACCAAGGAGATTTGGGAGGATCGCGGCATC
GCGACCCTGCACGAGTATTA

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MIDRAPITGKVTSIDVAQRAGVSQSAVSRVFTPGASVSKKMEARVREAADDLGYRPNVLARSLITGRSRIIGLVVAHLDN
SFYPDALEKLSAALQAKGYHILVFMAGNSGDDVDSVIHDLMDYQVDGIITASINLSGELTDRCRAAGLPVLLFNRGIEGS
GLSSVTSANRIGGAAVANTLLDAGHTKIATISGWMGASTGRDRRDGFVAALEARGAQVHAEADGLYDRDTAARVARDMMQ
AAHPPDAIFVGNDHMAMAVMDTLRFDLGLSVPDDVSIIGYDDVPMAAWPSYNLTTLRQPVNRMVEATVDTMLDEIETGRA
PGTRVEIEGELILRTSARLPKGPTI

Sequences:

>Translated_345_residues
MIDRAPITGKVTSIDVAQRAGVSQSAVSRVFTPGASVSKKMEARVREAADDLGYRPNVLARSLITGRSRIIGLVVAHLDN
SFYPDALEKLSAALQAKGYHILVFMAGNSGDDVDSVIHDLMDYQVDGIITASINLSGELTDRCRAAGLPVLLFNRGIEGS
GLSSVTSANRIGGAAVANTLLDAGHTKIATISGWMGASTGRDRRDGFVAALEARGAQVHAEADGLYDRDTAARVARDMMQ
AAHPPDAIFVGNDHMAMAVMDTLRFDLGLSVPDDVSIIGYDDVPMAAWPSYNLTTLRQPVNRMVEATVDTMLDEIETGRA
PGTRVEIEGELILRTSARLPKGPTI
>Mature_345_residues
MIDRAPITGKVTSIDVAQRAGVSQSAVSRVFTPGASVSKKMEARVREAADDLGYRPNVLARSLITGRSRIIGLVVAHLDN
SFYPDALEKLSAALQAKGYHILVFMAGNSGDDVDSVIHDLMDYQVDGIITASINLSGELTDRCRAAGLPVLLFNRGIEGS
GLSSVTSANRIGGAAVANTLLDAGHTKIATISGWMGASTGRDRRDGFVAALEARGAQVHAEADGLYDRDTAARVARDMMQ
AAHPPDAIFVGNDHMAMAVMDTLRFDLGLSVPDDVSIIGYDDVPMAAWPSYNLTTLRQPVNRMVEATVDTMLDEIETGRA
PGTRVEIEGELILRTSARLPKGPTI

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=335, Percent_Identity=29.5522388059701, Blast_Score=141, Evalue=5e-35,
Organism=Escherichia coli, GI1789068, Length=336, Percent_Identity=28.8690476190476, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1787948, Length=326, Percent_Identity=27.9141104294479, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1790369, Length=330, Percent_Identity=26.0606060606061, Blast_Score=118, Evalue=6e-28,
Organism=Escherichia coli, GI1788474, Length=306, Percent_Identity=27.4509803921569, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI1786540, Length=340, Percent_Identity=26.7647058823529, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787580, Length=280, Percent_Identity=24.6428571428571, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786268, Length=312, Percent_Identity=25.3205128205128, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1789202, Length=293, Percent_Identity=26.2798634812287, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787906, Length=253, Percent_Identity=28.4584980237154, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI48994940, Length=295, Percent_Identity=23.728813559322, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1790715, Length=273, Percent_Identity=21.978021978022, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1790192, Length=226, Percent_Identity=27.4336283185841, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36730; Mature: 36730

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDRAPITGKVTSIDVAQRAGVSQSAVSRVFTPGASVSKKMEARVREAADDLGYRPNVLA
CCCCCCCCCCEEEEHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHH
RSLITGRSRIIGLVVAHLDNSFYPDALEKLSAALQAKGYHILVFMAGNSGDDVDSVIHDL
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHH
MDYQVDGIITASINLSGELTDRCRAAGLPVLLFNRGIEGSGLSSVTSANRIGGAAVANTL
HHHCCCEEEEEEEECCCCHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHCCHHHHHHHH
LDAGHTKIATISGWMGASTGRDRRDGFVAALEARGAQVHAEADGLYDRDTAARVARDMMQ
HHCCCCEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCCCCHHHHHHHHHHHH
AAHPPDAIFVGNDHMAMAVMDTLRFDLGLSVPDDVSIIGYDDVPMAAWPSYNLTTLRQPV
HCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHH
NRMVEATVDTMLDEIETGRAPGTRVEIEGELILRTSARLPKGPTI
HHHHHHHHHHHHHHHHCCCCCCCEEEECCEEEEEECCCCCCCCCC
>Mature Secondary Structure
MIDRAPITGKVTSIDVAQRAGVSQSAVSRVFTPGASVSKKMEARVREAADDLGYRPNVLA
CCCCCCCCCCEEEEHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHH
RSLITGRSRIIGLVVAHLDNSFYPDALEKLSAALQAKGYHILVFMAGNSGDDVDSVIHDL
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHH
MDYQVDGIITASINLSGELTDRCRAAGLPVLLFNRGIEGSGLSSVTSANRIGGAAVANTL
HHHCCCEEEEEEEECCCCHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHCCHHHHHHHH
LDAGHTKIATISGWMGASTGRDRRDGFVAALEARGAQVHAEADGLYDRDTAARVARDMMQ
HHCCCCEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCCCCHHHHHHHHHHHH
AAHPPDAIFVGNDHMAMAVMDTLRFDLGLSVPDDVSIIGYDDVPMAAWPSYNLTTLRQPV
HCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHH
NRMVEATVDTMLDEIETGRAPGTRVEIEGELILRTSARLPKGPTI
HHHHHHHHHHHHHHHHCCCCCCCEEEECCEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA