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The map label for this gene is speE1 [H]

Identifier: 89053346

GI number: 89053346

Start: 810841

End: 812544

Strand: Direct

Name: speE1 [H]

Synonym: Jann_0855

Alternate gene names: 89053346

Gene position: 810841-812544 (Clockwise)

Preceding gene: 89053345

Following gene: 89053360

Centisome position: 18.78

GC content: 62.97

Gene sequence:

>1704_bases
GTGGATCGCCTACAAGATCATCGACATCGTGACACCCTTCCCGATCATCAAGGAGATCGAGGAGGATCAGAACACGTCCC
TGGCGGTCCTGATCGGGTCGGTCTTCCTGTCGATGTCGATCATCATCGCGGCTGTTATCCTGTCGTGAGCGAGGCCACAG
GGGGTCGTGCCGAGCTGACCCCAAAGGTTGCCTGGCTGCTGGCGGCCACGTTCATCGTGGCTGTCGCGGGCCTGATCTAT
GAGCTGATCGCGGCAACCATTTCCTCCTATCTGCTTGGCGATTCAATTCGTCAGTTTTCGTTCGTTATCGGCGTGTTCCT
TAGCGCGATGGGCGTGGGGGCCTGGGCCTCGCGGTTCGTGGGCGACGCGTTGTCGGGGTTTGTCCGCGCGCAGATCGCCC
TTGGGGCCATTGGCGGCGCGCTGGGGCCGGTGCTGTTCTTCGCCTATGCGATTTACGGCAATGTGGGCGTGCCTTTGTAC
GGTGGGTTGGTCGCCATTGGCATCCTGTCGGGGATGGAGATCCCCCTGATCTCCCGCGTGATGAAAGAGATCGGCGCGGC
GGAATTCCGGTTCGAGAATGTGCTGACCGCCGACTACATCGGCGCTTTGGCCGCCTCGCTGGCGTTTCCGCTGCTGATCG
TGCCGAACTTGCCGCTGATGTCTGCGTCGCTGGCCTTTGGCACGCTCAACCTGCTGGTCGCAGGGTTTTCGCTGTACCTG
TTCCGGGACCGCCTTGGTCGGGGCCTTTGGCTGCCTTGGGGCGCACTCCTCCTCGCTTCGCTCCTCGCATTCGTGATGAG
TGAGCGGATGGTCTCTGCCGTCGATGCGCGGCTGTTTCAGGACGAGGTGATCTTCTCAGAAACGACCGCCTACCAGAGTA
TCGTCATCACCCGGTTCGACGACCGCTACCGGCTGTTTCTGGATCATTCGATCCAGTTCGACAGTCTTGATGAGCACCGC
TACCACGAGGCGCTGGTGCATCCCGCCATGGGTCTCGTGCCCGATGCGCGACGCGTATTGGTGCTGGGGGGCGGTGACGG
CATGGCCGTGCGGGAGGTGTTGCGCCACGAGGGTGTGGAGCATGTGACGCTGGTCGATCTGGACCCCCGCGTGACGGAGT
TATTTGCCACGAACCCCGACCTTGTGGCTTTGAACGGCGGTGCGCTTGCGGATGCGCGGGTGGAGGTGGTGAACACCGAC
GCCTGGGTCTGGGCGGCGGAGGCAGACGGTGTATACGACGTCATCATCGCCGATCTGCCGGACCCGAAAAGCATCGCCCT
GTCCAAGCTCTACTCGGTGGAATTCTATGGCATGCTGGTGGAACGCTTGGCGGGCCAGGGGCTTCTGGTGACACAAGCGG
GCTCCCCCACCTTCGCGACGGAGGCGTTCTGGATCACGGAAGCGACCCTGGCCGCGACACGGAGCCCGGCAGACCCCGGC
GGGACGCTGCAAACCATCGCCTACCAGACCTATTTGCCAAGCTTCGGGAACTGGGGCTTCGTCATGGCCGGGCCGTTGCT
GCCCAATGACGCGCGAACGCCGGAGTTGCCCGAAGGATTGCGGCATATGACACCTGAGCTGTGGACCGCATCGCAAGTCT
TCGGCGTGGACCAGTCGCGCCCGGATGAGGTGCCGGTCAATACGATCCAGGGTCACCCGCTGGCGGCCGCCTATCAACGC
GGGTGGGAGTTCTGGTTCCGCTAA

Upstream 100 bases:

>100_bases
TTTGACCTTGGGAAAAGGAGCCCCTGCCCATGTTTGAAGCTATCGTCCTGGCTGAATTCGTCTCGATGCTGTTCTACACG
TTTGTGGGCATGCTGCTGTT

Downstream 100 bases:

>100_bases
GGCCGGTGCTGTTTGGGGCTCTGCCCCCGCCTGCGCCAGGGCGCAGCCTTCCCCCGGAGTTGTAACGGCCCAGATGAAAC
ATGGAGCGCTACGAATGGCA

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase 1; PAPT 1; SPDSY 1 [H]

Number of amino acids: Translated: 567; Mature: 567

Protein sequence:

>567_residues
MDRLQDHRHRDTLPDHQGDRGGSEHVPGGPDRVGLPVDVDHHRGCYPVVSEATGGRAELTPKVAWLLAATFIVAVAGLIY
ELIAATISSYLLGDSIRQFSFVIGVFLSAMGVGAWASRFVGDALSGFVRAQIALGAIGGALGPVLFFAYAIYGNVGVPLY
GGLVAIGILSGMEIPLISRVMKEIGAAEFRFENVLTADYIGALAASLAFPLLIVPNLPLMSASLAFGTLNLLVAGFSLYL
FRDRLGRGLWLPWGALLLASLLAFVMSERMVSAVDARLFQDEVIFSETTAYQSIVITRFDDRYRLFLDHSIQFDSLDEHR
YHEALVHPAMGLVPDARRVLVLGGGDGMAVREVLRHEGVEHVTLVDLDPRVTELFATNPDLVALNGGALADARVEVVNTD
AWVWAAEADGVYDVIIADLPDPKSIALSKLYSVEFYGMLVERLAGQGLLVTQAGSPTFATEAFWITEATLAATRSPADPG
GTLQTIAYQTYLPSFGNWGFVMAGPLLPNDARTPELPEGLRHMTPELWTASQVFGVDQSRPDEVPVNTIQGHPLAAAYQR
GWEFWFR

Sequences:

>Translated_567_residues
MDRLQDHRHRDTLPDHQGDRGGSEHVPGGPDRVGLPVDVDHHRGCYPVVSEATGGRAELTPKVAWLLAATFIVAVAGLIY
ELIAATISSYLLGDSIRQFSFVIGVFLSAMGVGAWASRFVGDALSGFVRAQIALGAIGGALGPVLFFAYAIYGNVGVPLY
GGLVAIGILSGMEIPLISRVMKEIGAAEFRFENVLTADYIGALAASLAFPLLIVPNLPLMSASLAFGTLNLLVAGFSLYL
FRDRLGRGLWLPWGALLLASLLAFVMSERMVSAVDARLFQDEVIFSETTAYQSIVITRFDDRYRLFLDHSIQFDSLDEHR
YHEALVHPAMGLVPDARRVLVLGGGDGMAVREVLRHEGVEHVTLVDLDPRVTELFATNPDLVALNGGALADARVEVVNTD
AWVWAAEADGVYDVIIADLPDPKSIALSKLYSVEFYGMLVERLAGQGLLVTQAGSPTFATEAFWITEATLAATRSPADPG
GTLQTIAYQTYLPSFGNWGFVMAGPLLPNDARTPELPEGLRHMTPELWTASQVFGVDQSRPDEVPVNTIQGHPLAAAYQR
GWEFWFR
>Mature_567_residues
MDRLQDHRHRDTLPDHQGDRGGSEHVPGGPDRVGLPVDVDHHRGCYPVVSEATGGRAELTPKVAWLLAATFIVAVAGLIY
ELIAATISSYLLGDSIRQFSFVIGVFLSAMGVGAWASRFVGDALSGFVRAQIALGAIGGALGPVLFFAYAIYGNVGVPLY
GGLVAIGILSGMEIPLISRVMKEIGAAEFRFENVLTADYIGALAASLAFPLLIVPNLPLMSASLAFGTLNLLVAGFSLYL
FRDRLGRGLWLPWGALLLASLLAFVMSERMVSAVDARLFQDEVIFSETTAYQSIVITRFDDRYRLFLDHSIQFDSLDEHR
YHEALVHPAMGLVPDARRVLVLGGGDGMAVREVLRHEGVEHVTLVDLDPRVTELFATNPDLVALNGGALADARVEVVNTD
AWVWAAEADGVYDVIIADLPDPKSIALSKLYSVEFYGMLVERLAGQGLLVTQAGSPTFATEAFWITEATLAATRSPADPG
GTLQTIAYQTYLPSFGNWGFVMAGPLLPNDARTPELPEGLRHMTPELWTASQVFGVDQSRPDEVPVNTIQGHPLAAAYQR
GWEFWFR

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG4262

COG function: function code R; Predicted spermidine synthase with an N-terminal membrane domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=242, Percent_Identity=25.6198347107438, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1786312, Length=252, Percent_Identity=30.1587301587302, Blast_Score=87, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI25154106, Length=239, Percent_Identity=30.9623430962343, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI212645616, Length=178, Percent_Identity=33.1460674157303, Blast_Score=76, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6323175, Length=268, Percent_Identity=27.6119402985075, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6325326, Length=186, Percent_Identity=30.6451612903226, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24645443, Length=260, Percent_Identity=31.1538461538462, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21355941, Length=207, Percent_Identity=30.9178743961353, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 61434; Mature: 61434

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1 ; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure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CCCCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12712204 [H]