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The map label for this gene is metK

Identifier: 89053197

GI number: 89053197

Start: 678196

End: 679377

Strand: Direct

Name: metK

Synonym: Jann_0706

Alternate gene names: 89053197

Gene position: 678196-679377 (Clockwise)

Preceding gene: 89053196

Following gene: 89053198

Centisome position: 15.71

GC content: 63.2

Gene sequence:

>1182_bases
ATGGCGCGTAACAATTATCTTTTCACCTCGGAATCCGTGTCCGAAGGGCATCCCGACAAAGTCTGTGACCGGATCTCGGA
CGCGATCTTGGACGCCTTCCTGTCGGAAGAGCCTGAGGCGCGCGTGGCCTGCGAGACCTTCGCCACCACCAACCGCGTTG
TGATCGGCGGTGAAGTGGGTCTGAGCGATCAGGACAAGCTGGCCGAATACATGGGCCAAGTGGACCCAATCGTGCGCGCC
TGCGTCAAGGACATCGGCTATGAGCAGGACAAGTTCCACCACGAGACCGTGGAAATCACCAACCTGTTGCACGAGCAATC
GGCCCATATCGCACAAGGTGTCGATGCGGCGGAGAACAAGGACGAAGGGGCTGGCGATCAAGGGATCATGTTCGGTTTTG
CCGTGGCAGAGACGCCTGAGTTGATGCCAGCGCCGATCCACTATGCCCACGCGATCCTCAAGAAACTGGCCGAGGTCCGC
AAATCCGGTGAGCAGGCGGTGCTTGGCCCCGACGCCAAGTCGCAACTCTCGGTGCGGTACGAGGATGGCACGCCCGTCGG
CGTCTCTTCCATCGTGTTGTCGACCCAGCATCTCGACGAGAGCCTGACGTCTGATGACGTGCGCGCCATCGTGGAGCCCT
ACATTCGCGGTGAGCTGCCCGATGGCTGGATCAGTGCCGAGACGGAATGGCACGTGAACCCCACAGGCAAGTTCGTGATC
GGCGGACCCGATGGCGATGCGGGCCTGACGGGCCGCAAGATCATCGTGGACACCTATGGCGGCGCGGCGCCCCATGGGGG
CGGTGCGTTTTCCGGCAAAGATCCGACCAAGGTGGACCGCTCGGCGGCCTACGCGTCGCGCTATCTGGCGAAGAATGTGG
TGGCGGCAGGCCTCGCGCATCGGTGCACGATCCAGCTCAGCTACGCGATCGGCGTGGCCAAGCCCCTGTCGATCTATTGC
GACACGCACCAGACCGGGCAGGTCCACGAGGCTGAGATTGAGCGCGCTTTGGGCCAGGTCATGGACCTGACACCGCGCGG
CATCCGCACGCATCTGGGCATGAACCGCCCCATCTATGAACGCACGGCGGCCTATGGCCACTTCGGCCGCGCGGCAGAGG
CCGATGGCGGGTTCTCGTGGGAAAAGACGGATCTGGCGGACGCGATCAAGGCGGCGCTCTAG

Upstream 100 bases:

>100_bases
TCCACACCCCATTGAATGCGGTGCAAAACGCGCGTATGCGACACCCGACTCCTCCACAACGGCTTCCTGACGTGGGGGAT
TATTTGAATGGAGCATTCCC

Downstream 100 bases:

>100_bases
GGCCGACTATCGCCACTTTGGCCTACAGTGATAAACTCCGGCTCTGACCCGTTTTGATCCGCCATGTGCCGGGCTTTTCC
GTTGGCCGATCTCCAGCTCG

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MARNNYLFTSESVSEGHPDKVCDRISDAILDAFLSEEPEARVACETFATTNRVVIGGEVGLSDQDKLAEYMGQVDPIVRA
CVKDIGYEQDKFHHETVEITNLLHEQSAHIAQGVDAAENKDEGAGDQGIMFGFAVAETPELMPAPIHYAHAILKKLAEVR
KSGEQAVLGPDAKSQLSVRYEDGTPVGVSSIVLSTQHLDESLTSDDVRAIVEPYIRGELPDGWISAETEWHVNPTGKFVI
GGPDGDAGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYASRYLAKNVVAAGLAHRCTIQLSYAIGVAKPLSIYC
DTHQTGQVHEAEIERALGQVMDLTPRGIRTHLGMNRPIYERTAAYGHFGRAAEADGGFSWEKTDLADAIKAAL

Sequences:

>Translated_393_residues
MARNNYLFTSESVSEGHPDKVCDRISDAILDAFLSEEPEARVACETFATTNRVVIGGEVGLSDQDKLAEYMGQVDPIVRA
CVKDIGYEQDKFHHETVEITNLLHEQSAHIAQGVDAAENKDEGAGDQGIMFGFAVAETPELMPAPIHYAHAILKKLAEVR
KSGEQAVLGPDAKSQLSVRYEDGTPVGVSSIVLSTQHLDESLTSDDVRAIVEPYIRGELPDGWISAETEWHVNPTGKFVI
GGPDGDAGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYASRYLAKNVVAAGLAHRCTIQLSYAIGVAKPLSIYC
DTHQTGQVHEAEIERALGQVMDLTPRGIRTHLGMNRPIYERTAAYGHFGRAAEADGGFSWEKTDLADAIKAAL
>Mature_392_residues
ARNNYLFTSESVSEGHPDKVCDRISDAILDAFLSEEPEARVACETFATTNRVVIGGEVGLSDQDKLAEYMGQVDPIVRAC
VKDIGYEQDKFHHETVEITNLLHEQSAHIAQGVDAAENKDEGAGDQGIMFGFAVAETPELMPAPIHYAHAILKKLAEVRK
SGEQAVLGPDAKSQLSVRYEDGTPVGVSSIVLSTQHLDESLTSDDVRAIVEPYIRGELPDGWISAETEWHVNPTGKFVIG
GPDGDAGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYASRYLAKNVVAAGLAHRCTIQLSYAIGVAKPLSIYCD
THQTGQVHEAEIERALGQVMDLTPRGIRTHLGMNRPIYERTAAYGHFGRAAEADGGFSWEKTDLADAIKAAL

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI5174529, Length=385, Percent_Identity=49.0909090909091, Blast_Score=329, Evalue=2e-90,
Organism=Homo sapiens, GI4557737, Length=385, Percent_Identity=48.3116883116883, Blast_Score=324, Evalue=7e-89,
Organism=Escherichia coli, GI1789311, Length=390, Percent_Identity=52.8205128205128, Blast_Score=398, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI25145633, Length=389, Percent_Identity=48.0719794344473, Blast_Score=334, Evalue=5e-92,
Organism=Caenorhabditis elegans, GI17538498, Length=390, Percent_Identity=48.4615384615385, Blast_Score=333, Evalue=1e-91,
Organism=Caenorhabditis elegans, GI17538494, Length=390, Percent_Identity=48.2051282051282, Blast_Score=333, Evalue=1e-91,
Organism=Caenorhabditis elegans, GI17551082, Length=387, Percent_Identity=46.5116279069767, Blast_Score=314, Evalue=4e-86,
Organism=Caenorhabditis elegans, GI32565905, Length=388, Percent_Identity=44.5876288659794, Blast_Score=295, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI32565911, Length=314, Percent_Identity=48.0891719745223, Blast_Score=263, Evalue=1e-70,
Organism=Caenorhabditis elegans, GI25145635, Length=313, Percent_Identity=47.2843450479233, Blast_Score=260, Evalue=1e-69,
Organism=Caenorhabditis elegans, GI32565903, Length=300, Percent_Identity=47.6666666666667, Blast_Score=250, Evalue=7e-67,
Organism=Caenorhabditis elegans, GI32565909, Length=88, Percent_Identity=50, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI32565907, Length=44, Percent_Identity=68.1818181818182, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6323209, Length=386, Percent_Identity=47.1502590673575, Blast_Score=318, Evalue=1e-87,
Organism=Saccharomyces cerevisiae, GI6320710, Length=386, Percent_Identity=45.5958549222798, Blast_Score=313, Evalue=2e-86,
Organism=Drosophila melanogaster, GI24580527, Length=385, Percent_Identity=48.0519480519481, Blast_Score=311, Evalue=3e-85,
Organism=Drosophila melanogaster, GI24580525, Length=385, Percent_Identity=48.0519480519481, Blast_Score=311, Evalue=3e-85,
Organism=Drosophila melanogaster, GI45552159, Length=385, Percent_Identity=48.0519480519481, Blast_Score=310, Evalue=9e-85,
Organism=Drosophila melanogaster, GI24580529, Length=385, Percent_Identity=48.0519480519481, Blast_Score=310, Evalue=9e-85,
Organism=Drosophila melanogaster, GI24580537, Length=385, Percent_Identity=48.0519480519481, Blast_Score=310, Evalue=9e-85,
Organism=Drosophila melanogaster, GI24580533, Length=385, Percent_Identity=48.0519480519481, Blast_Score=310, Evalue=9e-85,
Organism=Drosophila melanogaster, GI24580535, Length=385, Percent_Identity=48.0519480519481, Blast_Score=310, Evalue=9e-85,
Organism=Drosophila melanogaster, GI24580531, Length=385, Percent_Identity=48.0519480519481, Blast_Score=310, Evalue=9e-85,
Organism=Drosophila melanogaster, GI24580541, Length=386, Percent_Identity=45.8549222797927, Blast_Score=288, Evalue=3e-78,
Organism=Drosophila melanogaster, GI24580539, Length=125, Percent_Identity=45.6, Blast_Score=100, Evalue=3e-21,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_JANSC (Q28UI9)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_508648.1
- ProteinModelPortal:   Q28UI9
- SMR:   Q28UI9
- STRING:   Q28UI9
- GeneID:   3933149
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_0706
- eggNOG:   COG0192
- HOGENOM:   HBG443662
- OMA:   QDGFHWK
- ProtClustDB:   PRK05250
- BioCyc:   JSP290400:JANN_0706-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 42241; Mature: 42109

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARNNYLFTSESVSEGHPDKVCDRISDAILDAFLSEEPEARVACETFATTNRVVIGGEVG
CCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHEEHHHHCCCCEEEEECCCC
LSDQDKLAEYMGQVDPIVRACVKDIGYEQDKFHHETVEITNLLHEQSAHIAQGVDAAENK
CCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
DEGAGDQGIMFGFAVAETPELMPAPIHYAHAILKKLAEVRKSGEQAVLGPDAKSQLSVRY
CCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCEEEEE
EDGTPVGVSSIVLSTQHLDESLTSDDVRAIVEPYIRGELPDGWISAETEWHVNPTGKFVI
CCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCEEE
GGPDGDAGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYASRYLAKNVVAAGLAH
ECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RCTIQLSYAIGVAKPLSIYCDTHQTGQVHEAEIERALGQVMDLTPRGIRTHLGMNRPIYE
EEEEEEEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCHHH
RTAAYGHFGRAAEADGGFSWEKTDLADAIKAAL
HHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
ARNNYLFTSESVSEGHPDKVCDRISDAILDAFLSEEPEARVACETFATTNRVVIGGEVG
CCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHEEHHHHCCCCEEEEECCCC
LSDQDKLAEYMGQVDPIVRACVKDIGYEQDKFHHETVEITNLLHEQSAHIAQGVDAAENK
CCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
DEGAGDQGIMFGFAVAETPELMPAPIHYAHAILKKLAEVRKSGEQAVLGPDAKSQLSVRY
CCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCEEEEE
EDGTPVGVSSIVLSTQHLDESLTSDDVRAIVEPYIRGELPDGWISAETEWHVNPTGKFVI
CCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCEEE
GGPDGDAGLTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSAAYASRYLAKNVVAAGLAH
ECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RCTIQLSYAIGVAKPLSIYCDTHQTGQVHEAEIERALGQVMDLTPRGIRTHLGMNRPIYE
EEEEEEEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCHHH
RTAAYGHFGRAAEADGGFSWEKTDLADAIKAAL
HHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA